Restriction map for
pFN21AE3815
(Circular) (Six-base) MAPSORT of: pFN21AE3815.seq Check: 7989 from: 1 to: 6160
pFN21AE3815 6160 bp
With 182 enzymes: SgfI *
July 24, 2018 19:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2101 2101
Size: 6160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3503 1937
Size: 1566 4594
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4223 5607 5875 1632
Size: 2591 1384 268 1917
Fragments arranged by size:
2591 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3310 1184
Size: 2126 4034
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2740 2772 2740
Size: 32 6128
AlwNI CAG_nnn'CTG
Cuts at: 3903 5475 3903
Size: 1572 4588
ApaI G_GGCC'C
Cuts at: 3026 3051 3026
Size: 25 6135
ApaLI G'TGCA_C
Cuts at: 2102 2120 4217 5373 2102
Size: 18 2097 1156 2889
Fragments arranged by size:
2889 2097 1156 18
ApoI r'AATT_y
Cuts at: 1493 2165 3469 3695 3847 5956 1493
Size: 672 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 672 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 2269 2317 2444 2556 2635 2974
Size: 73 289 37 48 127 112 79 339
Cuts at: 2974 3373 3485 1870
Size: 399 112 4545
Fragments arranged by size:
4545 399 339 289 127 112 112 79
73 48 37
BamHI G'GATC_C
Cuts at: 2495 3490 3857 2495
Size: 995 367 4798
Fragments arranged by size:
4798 995 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2631 2653 3247 3274 3481
Size: 325 217 627 844 22 594 27 207
Cuts at: 3481 4936 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 844 627 594 325 217 207
27 22
BanII G_rGCy'C
Cuts at: 1391 2132 2181 2370 2791 3026 3051 3318 3329
Size: 741 49 189 421 235 25 267 11
Cuts at: 3329 3445 3479 1391
Size: 116 34 4072
Fragments arranged by size:
4072 741 421 267 235 189 116 49
34 25 11
BbeI G_GCGC'C
Cuts at: 2657 3251 3278 2657
Size: 594 27 5539
Fragments arranged by size:
5539 594 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5189
BbvCI CC'TCA_GC
Cuts at: 2115 2411 3422 2115
Size: 296 1011 4853
Fragments arranged by size:
4853 1011 296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4351 4385 1903
Size: 34 2414 34 3678
Fragments arranged by size:
3678 2414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2861 2918 3021
Size: 107 122 71 631 605 1189 57 103
Cuts at: 3021 3149 4771 136
Size: 128 1622 1525
Fragments arranged by size:
1622 1525 1189 631 605 128 122 107
103 71 57
BglII A'GATC_T
Cuts at: 2604 6155 2604
Size: 3551 2609
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2124 2775 3026 3051 4221 5377 1790
Size: 316 18 651 251 25 1170 1156 2573
Fragments arranged by size:
2573 1170 1156 651 316 251 25 18
BmgBI CAC'GTC
Cuts at: 1318 3096 1318
Size: 1778 4382
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3891 4849 442
Size: 416 53 83 399 24 2474 958 1753
Fragments arranged by size:
2474 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2890 3421 2890
Size: 531 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3248 3404 3470 4820 1239
Size: 2009 156 66 1350 2579
Fragments arranged by size:
2579 2009 1350 156 66
Bpu10I CC'TnA_GC
Cuts at: 2115 2411 3422 2115
Size: 296 1011 4853
Fragments arranged by size:
4853 1011 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3039 4285 5150 5448 3039
Size: 1246 865 298 3751
Fragments arranged by size:
3751 1246 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4823 882
Size: 617 3324 2219
Fragments arranged by size:
3324 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2770 3951 493
Size: 711 1566 1181 2702
Fragments arranged by size:
2702 1566 1181 711
BsaBI GATnn'nnATC
Cuts at: 2603 6154 2603
Size: 3551 2609
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2654 3204 3248 3275
Size: 53 83 186 936 1121 550 44 27
Cuts at: 3275 3967 4349 4984 275
Size: 692 382 635 1451
Fragments arranged by size:
1451 1121 936 692 635 550 382 186
83 53 44 27
BsaWI w'CCGG_w
Cuts at: 2362 2492 2926 4593 4991 5265 5412 5823 2362
Size: 130 434 1667 398 274 147 411 2699
Fragments arranged by size:
2699 1667 434 411 398 274 147 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5935
Fragments arranged by size:
5935 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 2576 2913 2946 3467 1186
Size: 1318 72 337 33 521 3879
Fragments arranged by size:
3879 1318 521 337 72 33
BseYI C'CCAG_C
Cuts at: 2846 3120 3186 5363 5859 2846
Size: 274 66 2177 496 3147
Fragments arranged by size:
3147 2177 496 274 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2073 2698 3270 1163
Size: 573 337 625 572 4053
Fragments arranged by size:
4053 625 573 572 337
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2174 3610 4371 5399 1540
Size: 275 186 173 1436 761 1028 2301
Fragments arranged by size:
2301 1436 1028 761 275 186 173
BsiHKAI G_wGCw'C
Cuts at: 2106 2124 3318 4221 4306 5377 2106
Size: 18 1194 903 85 1071 2889
Fragments arranged by size:
2889 1194 1071 903 85 18
BsmI GAATG_Cn'
Cuts at: 2358 3671 3764 2358
Size: 1313 93 4754
Fragments arranged by size:
4754 1313 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2443 2570 2443
Size: 127 6033
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2124 2132 2181 2370 2775 2791
Size: 399 316 18 8 49 189 405 16
Cuts at: 2791 3026 3051 3318 3329 3445 3479 4221 4306
Size: 235 25 267 11 116 34 742 85
Cuts at: 4306 5377 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 316 267 235
189 116 85 49 34 25 18 16
11 8
BspEI T'CCGG_A
Cuts at: 2362 2926 5823 2362
Size: 564 2897 2699
Fragments arranged by size:
2897 2699 564
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2101 2890 3515 844
Size: 1257 789 625 3489
Fragments arranged by size:
3489 1257 789 625
BsrBI CCG'CTC
Cuts at: 1394 1823 2305 3053 4047 1394
Size: 429 482 748 994 3507
Fragments arranged by size:
3507 994 748 482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4655 4829 65
Size: 1183 3407 174 1396
Fragments arranged by size:
3407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2134 2421 2469 2735 2860 3271 4804
Size: 110 324 287 48 266 125 411 1533
Cuts at: 4804 4991 1700
Size: 187 2869
Fragments arranged by size:
2869 1533 411 324 287 266 187 125
110 48
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6160
BssHII G'CGCG_C
Cuts at: 1925 2778 2891 1925
Size: 853 113 5194
Fragments arranged by size:
5194 853 113
BssSI C'ACGA_G
Cuts at: 2713 4220 5232 2713
Size: 1507 1012 3641
Fragments arranged by size:
3641 1507 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6160
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 2699 1184
Size: 1515 4645
BstYI r'GATC_y
Cuts at: 1972 2495 2604 2871 3490 3857 4242 4259 5711
Size: 523 109 267 619 367 385 17 1452
Cuts at: 5711 5797 5809 6155 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 619 523 385 367 346 267
109 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2949 2949
Size: 6160
BtgI C'CryG_G
Cuts at: 513 1065 2223 3110 513
Size: 552 1158 887 3563
Fragments arranged by size:
3563 1158 887 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2201 2308 2533 487
Size: 683 56 975 107 225 4114
Fragments arranged by size:
4114 975 683 225 107 56
BtsI GCAGTG_nn'
Cuts at: 2106 3676 4470 4490 2106
Size: 1570 794 20 3776
Fragments arranged by size:
3776 1570 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2171 2568 3607
Size: 54 1425 50 275 18 341 397 1039
Cuts at: 3607 4496 6125 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1039 889 397 341 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2171 3607 1537
Size: 275 359 1436 4090
Fragments arranged by size:
4090 1436 359 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3390 4090 1353
Size: 2037 700 3423
Fragments arranged by size:
3423 2037 700
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3199 4747 5121 5267 387
Size: 1064 138 1610 1548 374 146 1280
Fragments arranged by size:
1610 1548 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3316 3316
Size: 6160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3248 3404 3470 4223 4820 5607 5875
Size: 393 1616 156 66 753 597 787 268
Cuts at: 5875 1239
Size: 1524
Fragments arranged by size:
1616 1524 787 753 597 393 268 156
66
EcoNI CCTnn'n_nnAGG
Cuts at: 2040 3425 2040
Size: 1385 4775
EcoO109I rG'GnC_Cy
Cuts at: 2284 2998 3023 3131 3370 2284
Size: 714 25 108 239 5074
Fragments arranged by size:
5074 714 239 108 25
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2554 3098 2554
Size: 544 5616
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2657 2939 3251 3263 3278 5307 1678
Size: 979 282 312 12 15 2029 2531
Fragments arranged by size:
2531 2029 979 312 282 15 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 4801 4833 4875
Size: 32 135 32 669 32 2528 32 42
Cuts at: 4875 4907 1373
Size: 32 2626
Fragments arranged by size:
2626 2528 669 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3504 3750 669
Size: 988 281 1566 246 3079
Fragments arranged by size:
3079 1566 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6160
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KasI G'GCGC_C
Cuts at: 2653 3247 3274 2653
Size: 594 27 5539
Fragments arranged by size:
5539 594 27
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5274 5458 1305
Size: 3969 184 2007
Fragments arranged by size:
3969 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 10
Size: 54 1425 343 738 3600
Fragments arranged by size:
3600 1425 738 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 4118 4477 4636 518
Size: 648 1020 1932 359 159 2042
Fragments arranged by size:
2042 1932 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2225 2410 3576 4253 5401 5646 1606
Size: 619 185 1166 677 1148 245 2120
Fragments arranged by size:
2120 1166 1148 677 619 245 185
NaeI GCC'GGC
Cuts at: 2136 2471 2862 3273 2136
Size: 335 391 411 5023
Fragments arranged by size:
5023 411 391 335
NarI GG'CG_CC
Cuts at: 2654 3248 3275 2654
Size: 594 27 5539
Fragments arranged by size:
5539 594 27
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5608
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NgoMIV G'CCGG_C
Cuts at: 2134 2469 2860 3271 2134
Size: 335 391 411 5023
Fragments arranged by size:
5023 411 391 335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 3518 5063 1132
Size: 97 2289 1545 2229
Fragments arranged by size:
2289 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2370 3100 1348
Size: 1022 730 4408
Fragments arranged by size:
4408 1022 730
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PfoI T'CCnGG_A
Cuts at: 2586 2594 3264 2586
Size: 8 670 5482
Fragments arranged by size:
5482 670 8
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PmlI CAC'GTG
Cuts at: 2770 2770
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2998 3131 2998
Size: 133 6027
PshAI GACnn'nnGTC
Cuts at: 1613 2486 1613
Size: 873 5287
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PspOMI G'GGCC_C
Cuts at: 3022 3047 3022
Size: 25 6135
PstI C_TGCA'G
Cuts at: 830 2844 3342 3512 830
Size: 2014 498 170 3478
Fragments arranged by size:
3478 2014 498 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 2410 2410
Size: 6160
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 6160
SacI G_AGCT'C
Cuts at: 3318 3318
Size: 6160
SacII CC_GC'GG
Cuts at: 2226 2226
Size: 6160
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SanDI GG'GwC_CC
Cuts at: 2998 3131 2998
Size: 133 6027
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2840 3338 3508 4643 5324 5515 826
Size: 219 1795 498 170 1135 681 191 1471
Fragments arranged by size:
1795 1471 1135 681 498 219 191 170
SfiI GGCCn_nnn'nGGCC
Cuts at: 3021 3021
Size: 6160
SfoI GGC'GCC
Cuts at: 2655 3249 3276 2655
Size: 594 27 5539
Fragments arranged by size:
5539 594 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 2421 2735 1700
Size: 721 314 5125
Fragments arranged by size:
5125 721 314
SmaI CCC'GGG
Cuts at: 1872 2234 2319 2446 2558 2637 2976 3375 3487
Size: 362 85 127 112 79 339 399 112
Cuts at: 3487 1872
Size: 4545
Fragments arranged by size:
4545 399 362 339 127 112 112 85
79
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3054 4264 5165 5427 820
Size: 197 926 1111 1210 901 262 1553
Fragments arranged by size:
1553 1210 1111 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 2079 3389 1517
Size: 562 1310 4288
Fragments arranged by size:
4288 1310 562
StyI C'CwwG_G
Cuts at: 513 1065 3073 3599 513
Size: 552 2008 526 3074
Fragments arranged by size:
3074 2008 552 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3106 4187 4204 4357 4542 4987 325
Size: 1255 1526 1081 17 153 185 445 1498
Fragments arranged by size:
1526 1498 1255 1081 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4406 6118 96
Size: 274 80 33 51 1443 2429 1712 138
Fragments arranged by size:
2429 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6120 6152 6120
Size: 32 6128
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2338
Size: 6160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 2232 2317 2444 2556 2635 2974 3373 3485
Size: 362 85 127 112 79 339 399 112
Cuts at: 3485 1870
Size: 4545
Fragments arranged by size:
4545 399 362 339 127 112 112 85
79
XmnI GAAnn'nnTTC
Cuts at: 4289 4289
Size: 6160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI
PsrI SapI SexAI SrfI SwaI