Restriction map for
pFN21AE3811
(Circular) (Six-base) MAPSORT of: pFN21AE3811.seq Check: 247 from: 1 to: 5962
pFN21AE3811 5962 bp
With 182 enzymes: SgfI *
March 28, 2012 12:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2557 2616 4025 5409 5677 1632
Size: 925 59 1409 1384 268 1917
Fragments arranged by size:
1917 1409 1384 925 268 59
AfeI AGC'GCT
Cuts at: 2115 2115
Size: 5962
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 2227 2390 2450 3705 5277 2227
Size: 163 60 1255 1572 2912
Fragments arranged by size:
2912 1572 1255 163 60
ApaI G_GGCC'C
Cuts at: 2030 2030
Size: 5962
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2838 2953 3271 3497 3649 5758 1493
Size: 1345 115 318 226 152 2109 1697
Fragments arranged by size:
2109 1697 1345 318 226 152 115
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 3287 1870
Size: 73 1344 4545
Fragments arranged by size:
4545 1344 73
BamHI G'GATC_C
Cuts at: 2878 3292 3659 2878
Size: 414 367 5181
Fragments arranged by size:
5181 414 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2230 3283 4738 618
Size: 325 217 627 363 80 1053 1455 1842
Fragments arranged by size:
1842 1455 1053 627 363 325 217 80
BanII G_rGCy'C
Cuts at: 1391 2030 2142 3117 3281 1391
Size: 639 112 975 164 4072
Fragments arranged by size:
4072 975 639 164 112
BbeI G_GCGC'C
Cuts at: 2154 2234 2154
Size: 80 5882
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2584 928
Size: 971 685 4306
Fragments arranged by size:
4306 971 685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 2257 2788 3213 1548
Size: 709 531 425 4297
Fragments arranged by size:
4297 709 531 425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2030 3196 4023 5179 1790
Size: 240 1166 827 1156 2573
Fragments arranged by size:
2573 1166 1156 827 240
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2051 3234 1056
Size: 995 1183 3784
Fragments arranged by size:
3784 1183 995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2472 2531 4622 1239
Size: 1233 59 2091 2579
Fragments arranged by size:
2579 2091 1233 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3086 4087 4952 5250 3086
Size: 1001 865 298 3798
Fragments arranged by size:
3798 1001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 2231 3769 4151
Size: 53 83 186 936 618 80 1538 382
Cuts at: 4151 4786 275
Size: 635 1451
Fragments arranged by size:
1538 1451 936 635 618 382 186 83
80 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5737
Fragments arranged by size:
5737 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 3176 1186
Size: 1249 741 3972
Fragments arranged by size:
3972 1249 741
BseYI C'CCAG_C
Cuts at: 5165 5661 5165
Size: 496 5466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2326 2817 1163
Size: 573 417 173 491 4308
Fragments arranged by size:
4308 573 491 417 173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 2176 3412 4173 5201 1540
Size: 275 186 145 30 1236 761 1028 2301
Fragments arranged by size:
2301 1236 1028 761 275 186 145 30
BsiHKAI G_wGCw'C
Cuts at: 3117 4023 4108 5179 3117
Size: 906 85 1071 3900
Fragments arranged by size:
3900 1071 906 85
BsmI GAATG_Cn'
Cuts at: 2562 2978 3244 3473 3566 2562
Size: 416 266 229 93 4958
Fragments arranged by size:
4958 416 266 229 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2708 2708
Size: 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2142 3117 3196 3281 4023 4108
Size: 399 240 112 975 79 85 742 85
Cuts at: 4108 5179 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 975 742 399 240 112 85
85 79
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2469 3851 5581 2469
Size: 1382 1730 2850
Fragments arranged by size:
2850 1730 1382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2069 3317 844
Size: 1225 1248 3489
Fragments arranged by size:
3489 1248 1225
BsrBI CCG'CTC
Cuts at: 1394 1823 2158 3849 1394
Size: 429 335 1691 3507
Fragments arranged by size:
3507 1691 429 335
BsrDI GCAATG_nn'
Cuts at: 65 1248 3141 4457 4631 65
Size: 1183 1893 1316 174 1396
Fragments arranged by size:
1893 1396 1316 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 3044 4606 4793 1700
Size: 110 264 970 1562 187 2869
Fragments arranged by size:
2869 1562 970 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 2527 96
Size: 2431 3531
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 3076 4766 3076
Size: 1690 4272
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2878 3087 3292 3659 4044 4061 5513 5599
Size: 906 209 205 367 385 17 1452 86
Cuts at: 5599 5611 5957 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 906 385 367 346 209 205
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3102 3102
Size: 5962
BtgI C'CryG_G
Cuts at: 513 1065 2146 2176 2215 2245 2578 513
Size: 552 1081 30 39 30 333 3897
Fragments arranged by size:
3897 1081 552 333 39 30 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 3478 4272 4292 3478
Size: 794 20 5148
Fragments arranged by size:
5148 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 2350 3266 2350
Size: 916 5046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 2143 2218
Size: 54 1425 50 275 18 242 71 75
Cuts at: 2218 3409 4298 5927 8
Size: 1191 889 1629 43
Fragments arranged by size:
1629 1425 1191 889 275 242 75 71
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2143 3409 1537
Size: 275 331 1266 4090
Fragments arranged by size:
4090 1266 331 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3102 3892 1353
Size: 1749 790 3423
Fragments arranged by size:
3423 1749 790
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3115 3115
Size: 5962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2472 2531 2557 2616 4025 4622 5409
Size: 393 840 59 26 59 1409 597 787
Cuts at: 5409 5677 1239
Size: 268 1524
Fragments arranged by size:
1524 1409 840 787 597 393 268 59
59 26
EcoO109I rG'GnC_Cy
Cuts at: 2027 2935 2027
Size: 908 5054
EcoRI G'AATT_C
Cuts at: 2838 3271 3649 2838
Size: 433 378 5151
Fragments arranged by size:
5151 433 378
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 2117 2154 2185 2234 5109 1678
Size: 439 37 31 49 2875 2531
Fragments arranged by size:
2875 2531 439 49 37 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 3015 3047 3079
Size: 32 135 32 773 32 638 32 32
Cuts at: 3079 3111 4603 4635 4677 4709 1373
Size: 32 1492 32 42 32 2626
Fragments arranged by size:
2626 1492 773 638 135 42 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3016 3306 3552 669
Size: 988 281 1078 290 246 3079
Fragments arranged by size:
3079 1078 988 290 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3016 3552 3016
Size: 536 5426
KasI G'GCGC_C
Cuts at: 2150 2230 2150
Size: 80 5882
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2527 5076 5260 1305
Size: 1222 2549 184 2007
Fragments arranged by size:
2549 2007 1222 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 3920 4279 4438 518
Size: 648 957 1797 359 159 2042
Fragments arranged by size:
2042 1797 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2148 2178 2217 2236 3378 4055 5203 5448
Size: 542 30 39 19 1142 677 1148 245
Cuts at: 5448 1606
Size: 2120
Fragments arranged by size:
2120 1148 1142 677 542 245 39 30
19
NaeI GCC'GGC
Cuts at: 2076 3046 2076
Size: 970 4992
NarI GG'CG_CC
Cuts at: 2151 2231 2151
Size: 80 5882
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NgoMIV G'CCGG_C
Cuts at: 2074 3044 2074
Size: 970 4992
NheI G'CTAG_C
Cuts at: 1052 2047 3230 1052
Size: 995 1183 3784
Fragments arranged by size:
3784 1183 995
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2858 3320 4865 1132
Size: 97 1629 462 1545 2229
Fragments arranged by size:
2229 1629 1545 462 97
PasI CC'CwG_GG
Cuts at: 1348 2938 1348
Size: 1590 4372
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2227 2450 1108
Size: 1119 223 4620
Fragments arranged by size:
4620 1119 223
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PspOMI G'GGCC_C
Cuts at: 2026 2026
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2066 2331 3314 830
Size: 1236 265 983 3478
Fragments arranged by size:
3478 1236 983 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SacI G_AGCT'C
Cuts at: 3117 3117
Size: 5962
SacII CC_GC'GG
Cuts at: 2149 2179 2218 2149
Size: 30 39 5893
Fragments arranged by size:
5893 39 30
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 2105 4210 5922 2105
Size: 2105 1712 2145
Fragments arranged by size:
2145 2105 1712
SfcI C'TryA_G
Cuts at: 826 1045 2062 2327 2391 3310 4445 5126 5317
Size: 219 1017 265 64 919 1135 681 191
Cuts at: 5317 826
Size: 1471
Fragments arranged by size:
1471 1135 1017 919 681 265 219 191
64
SfoI GGC'GCC
Cuts at: 2152 2232 2152
Size: 80 5882
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 3044 1700
Size: 1344 4618
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3065 4066 4967 5229 820
Size: 197 926 1122 1001 901 262 1553
Fragments arranged by size:
1553 1122 1001 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2202 3058 3401 513
Size: 552 1137 856 343 3074
Fragments arranged by size:
3074 1137 856 552 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 2527 2963
Size: 274 80 33 51 1443 126 424 436
Cuts at: 2963 4208 5920 96
Size: 1245 1712 138
Fragments arranged by size:
1712 1443 1245 436 424 274 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 2570 4091 2570
Size: 1521 4441
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI
SrfI SwaI XcmI