Restriction map for
pFN21AE3810
(Circular) (Six-base) MAPSORT of: pFN21AE3810.seq Check: 9357 from: 1 to: 5926
pFN21AE3810 5926 bp
With 182 enzymes: SgfI *
March 28, 2012 12:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2721 3123 3269 1937
Size: 784 402 146 4594
Fragments arranged by size:
4594 784 402 146
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2056 2899 3989 5373 5641 1632
Size: 416 8 843 1090 1384 268 1917
Fragments arranged by size:
1917 1384 1090 843 416 268 8
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 2922 3204 4825 5802 1203
Size: 1719 282 1621 977 1327
Fragments arranged by size:
1719 1621 1327 977 282
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 2113 2173 3669 5241 2113
Size: 60 1496 1572 2798
Fragments arranged by size:
2798 1572 1496 60
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2312 3251 1870
Size: 73 369 939 4545
Fragments arranged by size:
4545 939 369 73
BamHI G'GATC_C
Cuts at: 2123 2308 3256 3623 2123
Size: 185 948 367 4426
Fragments arranged by size:
4426 948 367 185
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2383 2954 3141 3247
Size: 325 217 627 263 333 571 187 106
Cuts at: 3247 4702 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 571 333 325 263 217
187 106
BanII G_rGCy'C
Cuts at: 1391 2760 2994 3198 3245 1391
Size: 1369 234 204 47 4072
Fragments arranged by size:
4072 1369 234 204 47
BbeI G_GCGC'C
Cuts at: 2054 3145 2054
Size: 1091 4835
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4955
BbvCI CC'TCA_GC
Cuts at: 2483 2483
Size: 5926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4117 4151 1903
Size: 34 50 34 2096 34 3678
Fragments arranged by size:
3678 2096 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2777 4537 136
Size: 107 122 71 631 605 1105 1760 1525
Fragments arranged by size:
1760 1525 1105 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2851 5921 2851
Size: 3070 2856
BlpI GC'TnA_GC
Cuts at: 2231 2628 3343 2231
Size: 397 715 4814
Fragments arranged by size:
4814 715 397
Bme1580I G_kGCm'C
Cuts at: 1790 2388 3186 3987 5143 1790
Size: 598 798 801 1156 2573
Fragments arranged by size:
2573 1156 801 798 598
BmgBI CAC'GTC
Cuts at: 1318 2380 3207 1318
Size: 1062 827 4037
Fragments arranged by size:
4037 1062 827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3657 4615 442
Size: 416 53 83 399 24 2240 958 1753
Fragments arranged by size:
2240 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2690 4586 1239
Size: 1451 1896 2579
Fragments arranged by size:
2579 1896 1451
Bpu10I CC'TnA_GC
Cuts at: 2330 2483 2330
Size: 153 5773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2914 3068 4051 4916 5214 2429
Size: 485 154 983 865 298 3141
Fragments arranged by size:
3141 983 865 485 298 154
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3024 4589 882
Size: 617 1525 1565 2219
Fragments arranged by size:
2219 1565 1525 617
BsaAI yAC'GTr
Cuts at: 493 1204 3213 3717 493
Size: 711 2009 504 2702
Fragments arranged by size:
2702 2009 711 504
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 2346 3142 3733
Size: 53 83 186 936 518 295 796 591
Cuts at: 3733 4115 4750 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 796 635 591 518 382 295
186 83 53
BsaWI w'CCGG_w
Cuts at: 2361 4359 4757 5031 5178 5589 2361
Size: 1998 398 274 147 411 2698
Fragments arranged by size:
2698 1998 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2426 2456 2714 2744 2912
Size: 30 165 30 1023 30 258 30 168
Cuts at: 2912 2942 1178
Size: 30 4162
Fragments arranged by size:
4162 1023 258 168 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5926
BseYI C'CCAG_C
Cuts at: 2010 2548 2946 3185 5129 5625 2010
Size: 538 398 239 1944 496 2311
Fragments arranged by size:
2311 1944 538 496 398 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 2276 1163
Size: 573 531 9 4813
Fragments arranged by size:
4813 573 531 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2224 3376 4137 5165 1540
Size: 275 186 223 1152 761 1028 2301
Fragments arranged by size:
2301 1152 1028 761 275 223 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2237 2760 3987 4072 5143 2070
Size: 167 523 1227 85 1071 2853
Fragments arranged by size:
2853 1227 1071 523 167 85
BsmI GAATG_Cn'
Cuts at: 2038 2773 3437 3530 2038
Size: 735 664 93 4434
Fragments arranged by size:
4434 735 664 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2446 2599 2446
Size: 153 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2237 2388 2760 2994 3186 3198
Size: 399 280 167 151 372 234 192 12
Cuts at: 3198 3245 3987 4072 5143 1391
Size: 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 280 234 192
167 151 85 47 12
BspEI T'CCGG_A
Cuts at: 2361 5589 2361
Size: 3228 2698
BspHI T'CATG_A
Cuts at: 2196 3815 5545 2196
Size: 1619 1730 2577
Fragments arranged by size:
2577 1730 1619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 3042 3281 844
Size: 1376 822 239 3489
Fragments arranged by size:
3489 1376 822 239
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 4421 4595 65
Size: 1183 889 2284 174 1396
Fragments arranged by size:
2284 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2367 3167 4570 4757 1700
Size: 110 557 800 1403 187 2869
Fragments arranged by size:
2869 1403 800 557 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2173 2254 794
Size: 1379 81 4466
Fragments arranged by size:
4466 1379 81
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 2353 1184
Size: 1169 4757
BstYI r'GATC_y
Cuts at: 1972 2123 2308 2673 2851 3256 3623 4008 4025
Size: 151 185 365 178 405 367 385 17
Cuts at: 4025 5477 5563 5575 5921 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 405 385 367 365 346 185
178 151 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2162 2162
Size: 5926
BtgI C'CryG_G
Cuts at: 513 1065 2391 3048 3096 513
Size: 552 1326 657 48 3343
Fragments arranged by size:
3343 1326 657 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2306 2445 487
Size: 683 56 1080 139 3968
Fragments arranged by size:
3968 1080 683 139 56
BtsI GCAGTG_nn'
Cuts at: 3442 4236 4256 3442
Size: 794 20 5112
Fragments arranged by size:
5112 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2351 4933 809
Size: 507 1035 2582 1802
Fragments arranged by size:
2582 1802 1035 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 3373 4262
Size: 54 1425 50 275 18 357 1186 889
Cuts at: 4262 5891 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1186 889 357 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2765 2918 3047 3856 1353
Size: 1412 153 129 809 3423
Fragments arranged by size:
3423 1412 809 153 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2758 2758
Size: 5926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2056 2690 2899 3989 4586 5373
Size: 393 416 8 634 209 1090 597 787
Cuts at: 5373 5641 1239
Size: 268 1524
Fragments arranged by size:
1524 1090 787 634 597 416 393 268
209 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2161 2165 2303 2161
Size: 4 138 5784
Fragments arranged by size:
5784 138 4
EcoO109I rG'GnC_Cy
Cuts at: 3088 3088
Size: 5926
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2423 2423
Size: 5926
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2024 2054 3115 3145 5073 1678
Size: 346 30 1061 30 1928 2531
Fragments arranged by size:
2531 1928 1061 346 30 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2426 2458 4567
Size: 32 135 32 536 32 286 32 2109
Cuts at: 4567 4599 4641 4673 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2109 536 286 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2050 3141 2050
Size: 1091 4835
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2469 5040 5224 1305
Size: 1164 2571 184 2007
Fragments arranged by size:
2571 2007 1164 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 10
Size: 54 1425 343 357 3747
Fragments arranged by size:
3747 1425 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 2864 3884 4243 4402 518
Size: 648 1335 363 1020 359 159 2042
Fragments arranged by size:
2042 1335 1020 648 363 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2251 2971 3342 4019 5167 5412 1606
Size: 489 156 720 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 720 677 489 371 245 156
NaeI GCC'GGC
Cuts at: 3169 3169
Size: 5926
NarI GG'CG_CC
Cuts at: 2051 3142 2051
Size: 1091 4835
NcoI C'CATG_G
Cuts at: 513 1065 2391 3048 513
Size: 552 1326 657 3391
Fragments arranged by size:
3391 1326 657 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 3167 3167
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2506 2749 2926 3284 4829 1132
Size: 97 1277 243 177 358 1545 2229
Fragments arranged by size:
2229 1545 1277 358 243 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 2922 4825 2922
Size: 1903 4023
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5024
PfoI T'CCnGG_A
Cuts at: 2280 2298 2466 2676 3159 2280
Size: 18 168 210 483 5047
Fragments arranged by size:
5047 483 210 168 18
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2444 2476 2444
Size: 32 5894
PstI C_TGCA'G
Cuts at: 830 3039 3278 830
Size: 2209 239 3478
Fragments arranged by size:
3478 2209 239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2251 2251
Size: 5926
SacI G_AGCT'C
Cuts at: 2760 2760
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SapI GCTCTTCn'nnn_
Cuts at: 2765 3047 2765
Size: 282 5644
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 2462 3020 4174 5886 2462
Size: 558 1154 1712 2502
Fragments arranged by size:
2502 1712 1154 558
SfcI C'TryA_G
Cuts at: 826 1045 3035 3274 4409 5090 5281 826
Size: 219 1990 239 1135 681 191 1471
Fragments arranged by size:
1990 1471 1135 681 239 219 191
SfoI GGC'GCC
Cuts at: 2052 3143 2052
Size: 1091 4835
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2893 3083 4030 4931 5193
Size: 197 926 465 485 190 947 901 262
Cuts at: 5193 820
Size: 1553
Fragments arranged by size:
1553 947 926 901 485 465 262 197
190
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 2506 3284 1132
Size: 1374 778 3774
Fragments arranged by size:
3774 1374 778
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 2391 2838 3048 3365 513
Size: 552 1326 447 210 317 3074
Fragments arranged by size:
3074 1326 552 447 317 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2002 2238 2831 2833 2889 2953 3080
Size: 1255 422 236 593 2 56 64 127
Cuts at: 3080 3953 3970 4123 4308 4753 325
Size: 873 17 153 185 445 1498
Fragments arranged by size:
1498 1255 873 593 445 422 236 185
153 127 64 56 17 2
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 2412 2460
Size: 274 80 33 51 1443 81 354 48
Cuts at: 2460 2925 3018 4172 5884 96
Size: 465 93 1154 1712 138
Fragments arranged by size:
1712 1443 1154 465 354 274 138 93
81 80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 2438 1316
Size: 1122 4804
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2827 3103 2827
Size: 276 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII
SanDI SexAI SfiI SrfI SwaI