Restriction map for
pFN21AE3808
(Circular) (Six-base) MAPSORT of: pFN21AE3808.seq Check: 34 from: 1 to: 5782
pFN21AE3808 5782 bp
With 182 enzymes: SgfI *
March 28, 2012 12:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 3845 5229 5497 1632
Size: 605 1608 1384 268 1917
Fragments arranged by size:
1917 1608 1384 605 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2314 3525 5097 2314
Size: 1211 1572 2999
Fragments arranged by size:
2999 1572 1211
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2876 3107 1870
Size: 73 933 231 4545
Fragments arranged by size:
4545 933 231 73
AvrII C'CTAG_G
Cuts at: 2545 2545
Size: 5782
BamHI G'GATC_C
Cuts at: 2872 3112 3479 2872
Size: 240 367 5175
Fragments arranged by size:
5175 367 240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2614 3103 4558 618
Size: 325 217 627 827 489 1455 1842
Fragments arranged by size:
1842 1455 827 627 489 325 217
BanII G_rGCy'C
Cuts at: 1391 2156 2455 2628 2891 3079 3101 1391
Size: 765 299 173 263 188 22 4072
Fragments arranged by size:
4072 765 299 263 188 173 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 928
Size: 971 610 4201
Fragments arranged by size:
4201 971 610
BbvCI CC'TCA_GC
Cuts at: 2381 2793 2802 2381
Size: 412 9 5361
Fragments arranged by size:
5361 412 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2444 2662 4393
Size: 107 122 71 631 605 772 218 1731
Cuts at: 4393 136
Size: 1525
Fragments arranged by size:
1731 1525 772 631 605 218 122 107
71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 3843 4999 1790
Size: 2053 1156 2573
Fragments arranged by size:
2573 2053 1156
BmgBI CAC'GTC
Cuts at: 1318 2734 1318
Size: 1416 4366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2434 2519 2935
Size: 416 53 83 399 24 1017 85 416
Cuts at: 2935 3513 4471 442
Size: 578 958 1753
Fragments arranged by size:
1753 1017 958 578 416 416 399 85
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3019 3019
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2837 3032 4442 1239
Size: 1598 195 1410 2579
Fragments arranged by size:
2579 1598 1410 195
Bpu10I CC'TnA_GC
Cuts at: 2381 2622 2793 2802 2381
Size: 241 171 9 5361
Fragments arranged by size:
5361 241 171 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2989 3907 4772 5070 2989
Size: 918 865 298 3701
Fragments arranged by size:
3701 918 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 2544 4445 882
Size: 617 519 526 1901 2219
Fragments arranged by size:
2219 1901 617 526 519
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2142 4215 4613 4887 5034 5445 2142
Size: 2073 398 274 147 411 2479
Fragments arranged by size:
2479 2073 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3066 3096 1178
Size: 30 165 30 1663 30 3864
Fragments arranged by size:
3864 1663 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2128 2168 3008 3029 1186
Size: 877 65 40 840 21 3939
Fragments arranged by size:
3939 877 840 65 40 21
BseYI C'CCAG_C
Cuts at: 2149 2184 2775 2836 4985 5481 2149
Size: 35 591 61 2149 496 2450
Fragments arranged by size:
2450 2149 591 496 61 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2274 1163
Size: 573 538 4671
Fragments arranged by size:
4671 573 538
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2170 3232 3993 5021 1540
Size: 275 186 169 1062 761 1028 2301
Fragments arranged by size:
2301 1062 1028 761 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 2178 2455 2628 3079 3843 3928 4999 2178
Size: 277 173 451 764 85 1071 2961
Fragments arranged by size:
2961 1071 764 451 277 173 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
BsmBI CGTCTCn'nnnn_
Cuts at: 2290 2290
Size: 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2178 2455 2628 2891 3079 3101
Size: 399 366 22 277 173 263 188 22
Cuts at: 3101 3843 3928 4999 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 277 263 188
173 85 22 22
BspEI T'CCGG_A
Cuts at: 2142 5445 2142
Size: 3303 2479
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3137 844
Size: 2293 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 2101 2779 1184
Size: 917 678 4187
Fragments arranged by size:
4187 917 678
BstXI CCAn_nnnn'nTGG
Cuts at: 2817 2817
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2872 3112 3479 3864 3881 5333 5419 5431
Size: 900 240 367 385 17 1452 86 12
Cuts at: 5431 5777 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 900 385 367 346 240 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2031 2258 2031
Size: 227 5555
BtgI C'CryG_G
Cuts at: 513 1065 2004 2610 2670 513
Size: 552 939 606 60 3625
Fragments arranged by size:
3625 939 606 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 3298 4092 4112 2712
Size: 586 794 20 4382
Fragments arranged by size:
4382 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3031 3066 529
Size: 35 2467 35 3245
Fragments arranged by size:
3245 2467 35 35
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 2567 2663
Size: 54 1425 50 275 18 337 400 96
Cuts at: 2663 3229 4118 5747 8
Size: 566 889 1629 43
Fragments arranged by size:
1629 1425 889 566 400 337 275 96
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2167 3229 1537
Size: 275 355 1062 4090
Fragments arranged by size:
4090 1062 355 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2705 3712 1353
Size: 1352 1007 3423
Fragments arranged by size:
3423 1352 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2453 2626 3077 2453
Size: 173 451 5158
Fragments arranged by size:
5158 451 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2837 3032 3845 4442 5229 5497
Size: 393 605 600 195 813 597 787 268
Cuts at: 5497 1239
Size: 1524
Fragments arranged by size:
1524 813 787 605 600 597 393 268
195
EcoNI CCTnn'n_nnAGG
Cuts at: 2257 2257
Size: 5782
EcoO109I rG'GnC_Cy
Cuts at: 2016 2041 2321 2377 2404 2683 2951 2016
Size: 25 280 56 27 279 268 4847
Fragments arranged by size:
4847 280 279 268 56 27 25
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2973 4929 1678
Size: 1295 1956 2531
Fragments arranged by size:
2531 1956 1295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373
Size: 32 135 32 2851 32 42 32 2626
Fragments arranged by size:
2851 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2095 4896 5080 1305
Size: 790 2801 184 2007
Fragments arranged by size:
2801 2007 790 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2490 3740 4099 4258 518
Size: 648 1324 1250 359 159 2042
Fragments arranged by size:
2042 1324 1250 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2207 2280 2385 3198 3875 5023 5268 1606
Size: 601 73 105 813 677 1148 245 2120
Fragments arranged by size:
2120 1148 813 677 601 245 105 73
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4291
Fragments arranged by size:
4291 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2817 1348
Size: 1469 4313
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2077 1108
Size: 969 4813
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2321 2377 2951 2041
Size: 280 56 574 4872
Fragments arranged by size:
4872 574 280 56
PshAI GACnn'nnGTC
Cuts at: 1613 2556 1613
Size: 943 4839
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2310 2397 2534 3134 830
Size: 1480 87 137 600 3478
Fragments arranged by size:
3478 1480 600 137 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2207 2207
Size: 5782
SacI G_AGCT'C
Cuts at: 2455 2628 3079 2455
Size: 173 451 5158
Fragments arranged by size:
5158 451 173
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SanDI GG'GwC_CC
Cuts at: 2041 2041
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2306 2327 2393 2530 3130 4265 4946
Size: 219 1261 21 66 137 600 1135 681
Cuts at: 4946 5137 826
Size: 191 1471
Fragments arranged by size:
1471 1261 1135 681 600 219 191 137
66 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3004 3886 4787 5049 820
Size: 197 926 1061 882 901 262 1553
Fragments arranged by size:
1553 1061 926 901 882 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 2301 2765 1517
Size: 784 464 4534
Fragments arranged by size:
4534 784 464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2545 2726 3221 513
Size: 552 939 541 181 495 3074
Fragments arranged by size:
3074 939 552 541 495 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2310 2767 3809 3826 3979 4164 4609
Size: 1255 730 457 1042 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1498 1255 1042 730 457 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4028 5740 96
Size: 274 80 33 51 1443 2051 1712 138
Fragments arranged by size:
2051 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2886 2918 5742 5774 2886
Size: 32 2824 32 2894
Fragments arranged by size:
2894 2824 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2525 1316
Size: 1209 4573
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2814 2814
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI