Restriction map for
pFN21AE3805
(Circular) (Six-base) MAPSORT of: pFN21AE3805.seq Check: 5125 from: 1 to: 5395
pFN21AE3805 5395 bp
With 182 enzymes: SgfI *
March 28, 2012 12:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3205 278
Size: 53 83 186 936 1669 2468
Fragments arranged by size:
2468 1669 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2738 1937
Size: 801 4594
Acc65I G'GTAC_C
Cuts at: 2716 2716
Size: 5395
AclI AA'CG_TT
Cuts at: 1563 3522 3895 1563
Size: 1959 373 3063
Fragments arranged by size:
3063 1959 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2459 2700 3458 4842 5110 1632
Size: 461 366 241 758 1384 268 1917
Fragments arranged by size:
1917 1384 758 461 366 268 241
AfeI AGC'GCT
Cuts at: 2343 2397 2343
Size: 54 5341
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5198
AflIII A'CryG_T
Cuts at: 1203 4294 5271 1203
Size: 3091 977 1327
Fragments arranged by size:
3091 1327 977
AgeI A'CCGG_T
Cuts at: 4226 4226
Size: 5395
AhdI GACnn_n'nnGTC
Cuts at: 4124 4124
Size: 5395
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5395
AlwNI CAG_nnn'CTG
Cuts at: 3138 4710 3138
Size: 1572 3823
ApaI G_GGCC'C
Cuts at: 2484 2484
Size: 5395
ApaLI G'TGCA_C
Cuts at: 3452 4608 3452
Size: 1156 4239
ApoI r'AATT_y
Cuts at: 1493 2333 2704 2930 3082 5191 1493
Size: 840 371 226 152 2109 1697
Fragments arranged by size:
2109 1697 840 371 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3949 160
Size: 872 2917 1606
Fragments arranged by size:
2917 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5395
AvaI C'yCGr_G
Cuts at: 1870 1943 2720 1870
Size: 73 777 4545
Fragments arranged by size:
4545 777 73
BamHI G'GATC_C
Cuts at: 2356 2725 3092 2356
Size: 369 367 4659
Fragments arranged by size:
4659 369 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2716 4171 618
Size: 325 217 627 929 1455 1842
Fragments arranged by size:
1842 1455 929 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2484 2714 1391
Size: 1093 230 4072
Fragments arranged by size:
4072 1093 230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3586 3620 1903
Size: 34 50 34 1565 34 3678
Fragments arranged by size:
3678 1565 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3287 4497 3287
Size: 1210 4185
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4006 136
Size: 107 122 71 631 605 2334 1525
Fragments arranged by size:
2334 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2290 5390 2290
Size: 3100 2295
BlpI GC'TnA_GC
Cuts at: 2034 2812 2034
Size: 778 4617
Bme1580I G_kGCm'C
Cuts at: 1790 2484 3456 4612 1790
Size: 694 972 1156 2573
Fragments arranged by size:
2573 1156 972 694
BmgBI CAC'GTC
Cuts at: 1318 2116 1318
Size: 798 4597
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3126 4084 442
Size: 416 53 83 399 24 1709 958 1753
Fragments arranged by size:
1753 1709 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4055 1239
Size: 2816 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2214 2315 3520 4385 4683 2214
Size: 101 1205 865 298 2926
Fragments arranged by size:
2926 1205 865 298 101
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4058 882
Size: 617 2559 2219
Fragments arranged by size:
2559 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3186 493
Size: 711 1982 2702
Fragments arranged by size:
2702 1982 711
BsaBI GATnn'nnATC
Cuts at: 5389 5389
Size: 5395
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2601 3202 3584 4219
Size: 53 83 186 936 1068 601 382 635
Cuts at: 4219 275
Size: 1451
Fragments arranged by size:
1451 1068 936 635 601 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3828 4226 4500 4647 5058 3828
Size: 398 274 147 411 4165
Fragments arranged by size:
4165 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5170
Fragments arranged by size:
5170 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2172 1186
Size: 986 4409
BseYI C'CCAG_C
Cuts at: 2103 4598 5094 2103
Size: 2495 496 2404
Fragments arranged by size:
2495 2404 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4822
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 2470 2845 3606 4634 1540
Size: 275 186 111 358 375 761 1028 2301
Fragments arranged by size:
2301 1028 761 375 358 275 186 111
BsiHKAI G_wGCw'C
Cuts at: 3456 3541 4612 3456
Size: 85 1071 4239
Fragments arranged by size:
4239 1071 85
BsmI GAATG_Cn'
Cuts at: 2906 2999 2906
Size: 93 5302
BsmBI CGTCTCn'nnnn_
Cuts at: 2555 2555
Size: 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2484 2714 3456 3541 4612 1391
Size: 399 694 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 694 399 230 85
BspEI T'CCGG_A
Cuts at: 5058 5058
Size: 5395
BspHI T'CATG_A
Cuts at: 3284 5014 3284
Size: 1730 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2750 844
Size: 1906 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2486 3282 1394
Size: 429 663 796 3507
Fragments arranged by size:
3507 796 663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3890 4064 65
Size: 1183 2642 174 1396
Fragments arranged by size:
2642 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 4039 4226 1700
Size: 110 297 1932 187 2869
Fragments arranged by size:
2869 1932 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5395
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5395
BssSI C'ACGA_G
Cuts at: 3455 4467 3455
Size: 1012 4383
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5395
BstBI TT'CG_AA
Cuts at: 4199 4199
Size: 5395
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5395
BstYI r'GATC_y
Cuts at: 1972 2040 2161 2290 2356 2454 2725 3092 3477
Size: 68 121 129 66 98 271 367 385
Cuts at: 3477 3494 4946 5032 5044 5390 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 271 129 121
98 86 68 66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2130 2196 513
Size: 552 1065 66 3712
Fragments arranged by size:
3712 1065 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4656
Fragments arranged by size:
4656 683 56
BtsI GCAGTG_nn'
Cuts at: 2264 2430 2911 3705 3725 2264
Size: 166 481 794 20 3934
Fragments arranged by size:
3934 794 481 166 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5395
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5360
DraI TTT'AAA
Cuts at: 2699 2699
Size: 5395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4402 809
Size: 507 3086 1802
Fragments arranged by size:
3086 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2529 2842
Size: 54 1425 50 275 18 279 420 313
Cuts at: 2842 3731 5360 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 420 313 279 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 2842 1537
Size: 275 297 733 4090
Fragments arranged by size:
4090 733 297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3325 1353
Size: 1972 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3982 4356 4502 387
Size: 1064 138 2393 374 146 1280
Fragments arranged by size:
2393 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2459 2700 3458 4055 4842 5110
Size: 393 461 366 241 758 597 787 268
Cuts at: 5110 1239
Size: 1524
Fragments arranged by size:
1524 787 758 597 461 393 366 268
241
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 5395
EcoO109I rG'GnC_Cy
Cuts at: 2421 2421
Size: 5395
EcoRI G'AATT_C
Cuts at: 2704 3082 2704
Size: 378 5017
FspI TGC'GCA
Cuts at: 3901 3901
Size: 5395
HaeII r_GCGC'y
Cuts at: 1678 2345 2399 4542 1678
Size: 667 54 2143 2531
Fragments arranged by size:
2531 2143 667 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 2552 2584 4036
Size: 32 135 32 574 32 374 32 1452
Cuts at: 4036 4068 4110 4142 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1452 574 374 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2739 2985 669
Size: 988 281 801 246 3079
Fragments arranged by size:
3079 988 801 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5395
HpaI GTT'AAC
Cuts at: 2985 2985
Size: 5395
KpnI G_GTAC'C
Cuts at: 2720 2720
Size: 5395
MfeI C'AATT_G
Cuts at: 2994 2994
Size: 5395
MluI A'CGCG_T
Cuts at: 5271 5271
Size: 5395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4509 4693 1305
Size: 3204 184 2007
Fragments arranged by size:
3204 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 10
Size: 54 1425 343 699 2874
Fragments arranged by size:
2874 1425 699 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3353 3712 3871 518
Size: 648 2187 359 159 2042
Fragments arranged by size:
2187 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2132 2811 3488 4636 4881 1606
Size: 526 679 677 1148 245 2120
Fragments arranged by size:
2120 1148 679 677 526 245
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 5395
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4843
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5395
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 5395
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5395
NotI GC'GGCC_GC
Cuts at: 2842 2842
Size: 5395
NspI r_CATG'y
Cuts at: 1132 1229 2413 2753 4298 1132
Size: 97 1184 340 1545 2229
Fragments arranged by size:
2229 1545 1184 340 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5395
PciI A'CATG_T
Cuts at: 4294 4294
Size: 5395
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5395
PmeI GTTT'AAAC
Cuts at: 2699 2699
Size: 5395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 4977 5009 3464
Size: 32 1481 32 3850
Fragments arranged by size:
3850 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2421 2421
Size: 5395
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5395
PsiI TTA'TAA
Cuts at: 2965 2965
Size: 5395
PspOMI G'GGCC_C
Cuts at: 2480 2480
Size: 5395
PstI C_TGCA'G
Cuts at: 830 2747 830
Size: 1917 3478
PvuI CG_AT'CG
Cuts at: 2001 2470 2001
Size: 469 4926
SacII CC_GC'GG
Cuts at: 2133 2133
Size: 5395
SalI G'TCGA_C
Cuts at: 1936 2737 1936
Size: 801 4594
SbfI CC_TGCA'GG
Cuts at: 2747 2747
Size: 5395
ScaI AGT'ACT
Cuts at: 3643 5355 3643
Size: 1712 3683
SfcI C'TryA_G
Cuts at: 826 1045 2743 3878 4559 4750 826
Size: 219 1698 1135 681 191 1471
Fragments arranged by size:
1698 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5395
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5395
SmaI CCC'GGG
Cuts at: 1872 2722 1872
Size: 850 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2229 2294 3499 4400 4662 820
Size: 197 926 286 65 1205 901 262 1553
Fragments arranged by size:
1553 1205 926 901 286 262 197 65
SnaBI TAC'GTA
Cuts at: 493 3186 493
Size: 2693 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5395
SphI G_CATG'C
Cuts at: 1132 2413 2753 1132
Size: 1281 340 3774
Fragments arranged by size:
3774 1281 340
SspI AAT'ATT
Cuts at: 5 52 3319 5
Size: 47 3267 2081
Fragments arranged by size:
3267 2081 47
StuI AGG'CCT
Cuts at: 1517 2223 1517
Size: 706 4689
StyI C'CwwG_G
Cuts at: 513 1065 2085 2556 2834 513
Size: 552 1020 471 278 3074
Fragments arranged by size:
3074 1020 552 471 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 3422 3439 3592 3777 4222 325
Size: 1255 537 1305 17 153 185 445 1498
Fragments arranged by size:
1498 1305 1255 537 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3641 5353 96
Size: 274 80 33 51 1443 1664 1712 138
Fragments arranged by size:
1712 1664 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5355 5387 5355
Size: 32 5363
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5395
XbaI T'CTAG_A
Cuts at: 2731 2731
Size: 5395
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5395
XmaI C'CCGG_G
Cuts at: 1870 2720 1870
Size: 850 4545
XmnI GAAnn'nnTTC
Cuts at: 2078 3524 2078
Size: 1446 3949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3203 276
Size: 53 83 186 936 1669 2468
Fragments arranged by size:
2468 1669 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI