Restriction map for
pFN21AE3770
(Circular) (Six-base) MAPSORT of: pFN21AE3770.seq Check: 9471 from: 1 to: 7687
pFN21AE3770 7687 bp
With 182 enzymes: SgfI *
March 28, 2012 12:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3344 5497 278
Size: 53 83 186 936 1808 2153 2468
Fragments arranged by size:
2468 2153 1808 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2692 5030 1937
Size: 755 2338 4594
Fragments arranged by size:
4594 2338 755
Acc65I G'GTAC_C
Cuts at: 5008 5008
Size: 7687
AclI AA'CG_TT
Cuts at: 1563 5814 6187 1563
Size: 4251 373 3063
Fragments arranged by size:
4251 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2335 2393 3834 4039 5750 7134 7402 1632
Size: 703 58 1441 205 1711 1384 268 1917
Fragments arranged by size:
1917 1711 1441 1384 703 268 205 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7490
AflIII A'CryG_T
Cuts at: 1203 6586 7563 1203
Size: 5383 977 1327
Fragments arranged by size:
5383 1327 977
AgeI A'CCGG_T
Cuts at: 6518 6518
Size: 7687
AhdI GACnn_n'nnGTC
Cuts at: 6416 6416
Size: 7687
AleI CACnn'nnGTG
Cuts at: 1166 2666 1166
Size: 1500 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2309 1184
Size: 1125 6562
AlwNI CAG_nnn'CTG
Cuts at: 4036 5430 7002 4036
Size: 1394 1572 4721
Fragments arranged by size:
4721 1572 1394
ApaI G_GGCC'C
Cuts at: 2053 2853 4840 2053
Size: 800 1987 4900
Fragments arranged by size:
4900 1987 800
ApaLI G'TGCA_C
Cuts at: 3163 4853 5744 6900 3163
Size: 1690 891 1156 3950
Fragments arranged by size:
3950 1690 1156 891
ApoI r'AATT_y
Cuts at: 1493 4212 4996 5222 5374 7483 1493
Size: 2719 784 226 152 2109 1697
Fragments arranged by size:
2719 2109 1697 784 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6241 160
Size: 872 5209 1606
Fragments arranged by size:
5209 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7687
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 2052 2147 4588 5012 1870
Size: 73 103 6 95 2441 424 4545
Fragments arranged by size:
4545 2441 424 103 95 73 6
BamHI G'GATC_C
Cuts at: 2220 5017 5384 2220
Size: 2797 367 4523
Fragments arranged by size:
4523 2797 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 2076 3113 3937 4562
Size: 325 217 627 226 63 1037 824 625
Cuts at: 4562 5008 6463 618
Size: 446 1455 1842
Fragments arranged by size:
1842 1455 1037 824 627 625 446 325
226 217 63
BanII G_rGCy'C
Cuts at: 1391 2053 2853 4840 5006 1391
Size: 662 800 1987 166 4072
Fragments arranged by size:
4072 1987 800 662 166
BbeI G_GCGC'C
Cuts at: 2017 2017
Size: 7687
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2955 3101 3705 4190 928
Size: 971 1056 146 604 485 4425
Fragments arranged by size:
4425 1056 971 604 485 146
BbvCI CC'TCA_GC
Cuts at: 3686 3686
Size: 7687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2498 2532 5878 5912 1903
Size: 34 50 34 477 34 3346 34 3678
Fragments arranged by size:
3678 3346 477 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2244 2420 3516 5579 6789 2244
Size: 176 1096 2063 1210 3142
Fragments arranged by size:
3142 2063 1210 1096 176
BclI T'GATC_A
Cuts at: 1548 3930 1548
Size: 2382 5305
BfrBI ATG'CAT
Cuts at: 2444 4133 4231 2444
Size: 1689 98 5900
Fragments arranged by size:
5900 1689 98
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6298 136
Size: 107 122 71 631 605 4626 1525
Fragments arranged by size:
4626 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7682 7682
Size: 7687
BlpI GC'TnA_GC
Cuts at: 5104 5104
Size: 7687
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2853 3167 3326 4565 4840 4857 5748
Size: 263 800 314 159 1239 275 17 891
Cuts at: 5748 6904 1790
Size: 1156 2573
Fragments arranged by size:
2573 1239 1156 891 800 314 275 263
159 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7687
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5418 6376 442
Size: 416 53 83 399 24 4001 958 1753
Fragments arranged by size:
4001 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4767 1056
Size: 3711 3976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2488 2956 4133 4550 4967 6347 1239
Size: 1124 125 468 1177 417 417 1380 2579
Fragments arranged by size:
2579 1380 1177 1124 468 417 417 125
Bpu10I CC'TnA_GC
Cuts at: 2216 3686 3796 3879 4407 2216
Size: 1470 110 83 528 5496
Fragments arranged by size:
5496 1470 528 110 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4573 5812 6677 6975 4573
Size: 1239 865 298 5285
Fragments arranged by size:
5285 1239 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 6350 882
Size: 617 1023 3828 2219
Fragments arranged by size:
3828 2219 1023 617
BsaAI yAC'GTr
Cuts at: 493 1204 2431 4120 5478 493
Size: 711 1227 1689 1358 2702
Fragments arranged by size:
2702 1689 1358 1227 711
BsaBI GATnn'nnATC
Cuts at: 7681 7681
Size: 7687
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2014 3341 5494 5876
Size: 53 83 186 936 481 1327 2153 382
Cuts at: 5876 6511 275
Size: 635 1451
Fragments arranged by size:
2153 1451 1327 936 635 481 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2926 6120 6518 6792 6939 7350 2926
Size: 3194 398 274 147 411 3263
Fragments arranged by size:
3263 3194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7462
Fragments arranged by size:
7462 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3351 3785 1186
Size: 2165 434 5088
Fragments arranged by size:
5088 2165 434
BseYI C'CCAG_C
Cuts at: 2684 3365 4043 6890 7386 2684
Size: 681 678 2847 496 2985
Fragments arranged by size:
2985 2847 681 678 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2751 3064 3227 4401 4934 1163
Size: 573 1015 313 163 1174 533 3916
Fragments arranged by size:
3916 1174 1015 573 533 313 163
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2089 5137 5898 6926 1540
Size: 275 186 88 3048 761 1028 2301
Fragments arranged by size:
3048 2301 1028 761 275 186 88
BsiHKAI G_wGCw'C
Cuts at: 3167 4119 4583 4857 5748 5833 6904 3167
Size: 952 464 274 891 85 1071 3950
Fragments arranged by size:
3950 1071 952 891 464 274 85
BsmI GAATG_Cn'
Cuts at: 5198 5291 5198
Size: 93 7594
BsmBI CGTCTCn'nnnn_
Cuts at: 2051 2051
Size: 7687
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2853 3167 3326 4119 4565 4583
Size: 399 263 800 314 159 793 446 18
Cuts at: 4583 4840 4857 5006 5748 5833 6904 1391
Size: 257 17 149 742 85 1071 2174
Fragments arranged by size:
2174 1071 800 793 742 446 399 314
263 257 159 149 85 18 17
BspEI T'CCGG_A
Cuts at: 2926 7350 2926
Size: 4424 3263
BspHI T'CATG_A
Cuts at: 2475 2812 5576 7306 2475
Size: 337 2764 1730 2856
Fragments arranged by size:
2856 2764 1730 337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4956 5042 844
Size: 4112 86 3489
Fragments arranged by size:
4112 3489 86
BsrBI CCG'CTC
Cuts at: 1394 1823 5574 1394
Size: 429 3751 3507
Fragments arranged by size:
3751 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2780 6182 6356 65
Size: 1183 1532 3402 174 1396
Fragments arranged by size:
3402 1532 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 2343 6331 6518 1700
Size: 110 254 279 3988 187 2869
Fragments arranged by size:
3988 2869 279 254 187 110
BsrGI T'GTAC_A
Cuts at: 96 3781 96
Size: 3685 4002
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7687
BssSI C'ACGA_G
Cuts at: 5747 6759 5747
Size: 1012 6675
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4036 794
Size: 3242 4445
BstBI TT'CG_AA
Cuts at: 6491 6491
Size: 7687
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7687
BstXI CCAn_nnnn'nTGG
Cuts at: 2668 2668
Size: 7687
BstYI r'GATC_y
Cuts at: 1972 2220 2382 5017 5384 5769 5786 7238 7324
Size: 248 162 2635 367 385 17 1452 86
Cuts at: 7324 7336 7682 1972
Size: 12 346 1977
Fragments arranged by size:
2635 1977 1452 385 367 346 248 162
86 17 12
BstZ17I GTA'TAC
Cuts at: 2693 2693
Size: 7687
Bsu36I CC'TnA_GG
Cuts at: 3021 3200 3281 3021
Size: 179 81 7427
Fragments arranged by size:
7427 179 81
BtgI C'CryG_G
Cuts at: 513 1065 2422 2845 513
Size: 552 1357 423 5355
Fragments arranged by size:
5355 1357 552 423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6948
Fragments arranged by size:
6948 683 56
BtsI GCAGTG_nn'
Cuts at: 3081 5203 5997 6017 3081
Size: 2122 794 20 4751
Fragments arranged by size:
4751 2122 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2229 2264 529
Size: 35 1665 35 5952
Fragments arranged by size:
5952 1665 35 35
DraI TTT'AAA
Cuts at: 4192 4991 4192
Size: 799 6888
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6694 809
Size: 507 5378 1802
Fragments arranged by size:
5378 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2496 3300
Size: 54 1425 50 275 18 267 399 804
Cuts at: 3300 3961 5134 6023 7652 8
Size: 661 1173 889 1629 43
Fragments arranged by size:
1629 1425 1173 889 804 661 399 275
267 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5134 1537
Size: 275 3322 4090
Fragments arranged by size:
4090 3322 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2354 2565 3556 4058 5617 1353
Size: 1001 211 991 502 1559 3423
Fragments arranged by size:
3423 1559 1001 991 502 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2046 6274 6648 6794 387
Size: 1064 138 457 4228 374 146 1280
Fragments arranged by size:
4228 1280 1064 457 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2335 2363 2393 2488 2956 3834 4039
Size: 393 703 28 30 95 468 878 205
Cuts at: 4039 4133 4550 4967 5750 6347 7134 7402 1239
Size: 94 417 417 783 597 787 268 1524
Fragments arranged by size:
1524 878 787 783 703 597 468 417
417 393 268 205 95 94 30 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3378 4288 3378
Size: 910 6777
EcoO109I rG'GnC_Cy
Cuts at: 2304 2827 2850 3825 4827 4836 2304
Size: 523 23 975 1002 9 5155
Fragments arranged by size:
5155 1002 975 523 23 9
EcoRI G'AATT_C
Cuts at: 4996 5374 4996
Size: 378 7309
EcoRV GAT'ATC
Cuts at: 2614 2614
Size: 7687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4204 4204
Size: 7687
FspI TGC'GCA
Cuts at: 6193 6193
Size: 7687
HaeII r_GCGC'y
Cuts at: 1678 2017 3225 4036 6834 1678
Size: 339 1208 811 2798 2531
Fragments arranged by size:
2798 2531 1208 811 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2519 2551 2623 2655 2960
Size: 32 135 32 947 32 72 32 305
Cuts at: 2960 2992 3196 3228 3669 3701 6328 6360 6402
Size: 32 204 32 441 32 2627 32 42
Cuts at: 6402 6434 1373
Size: 32 2626
Fragments arranged by size:
2627 2626 947 441 305 204 135 72
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5031 5277 669
Size: 988 281 3093 246 3079
Fragments arranged by size:
3093 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7687
HpaI GTT'AAC
Cuts at: 5277 5277
Size: 7687
KasI G'GCGC_C
Cuts at: 2013 2013
Size: 7687
KpnI G_GTAC'C
Cuts at: 5012 5012
Size: 7687
MfeI C'AATT_G
Cuts at: 5286 5286
Size: 7687
MluI A'CGCG_T
Cuts at: 7563 7563
Size: 7687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2261 2439 2781 4092 6801 6985 1305
Size: 956 178 342 1311 2709 184 2007
Fragments arranged by size:
2709 2007 1311 956 342 184 178
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 3302 3963 10
Size: 54 1425 343 666 804 661 3734
Fragments arranged by size:
3734 1425 804 666 661 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2666 2964 3132 3251 4377 5645 6004
Size: 648 1500 298 168 119 1126 1268 359
Cuts at: 6004 6163 518
Size: 159 2042
Fragments arranged by size:
2042 1500 1268 1126 648 359 298 168
159 119
MspA1I CmG'CkG
Cuts at: 1606 2085 2112 3541 5103 5780 6928 7173 1606
Size: 479 27 1429 1562 677 1148 245 2120
Fragments arranged by size:
2120 1562 1429 1148 677 479 245 27
NaeI GCC'GGC
Cuts at: 2066 2066
Size: 7687
NarI GG'CG_CC
Cuts at: 2014 2014
Size: 7687
NcoI C'CATG_G
Cuts at: 513 1065 2845 513
Size: 552 1780 5355
Fragments arranged by size:
5355 1780 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7687
NgoMIV G'CCGG_C
Cuts at: 2064 2064
Size: 7687
NheI G'CTAG_C
Cuts at: 1052 4763 1052
Size: 3711 3976
NotI GC'GGCC_GC
Cuts at: 5134 5134
Size: 7687
NsiI A_TGCA'T
Cuts at: 2446 4135 4233 2446
Size: 1689 98 5900
Fragments arranged by size:
5900 1689 98
NspI r_CATG'y
Cuts at: 1132 1229 3090 3250 3706 3782 4133 4235 4254
Size: 97 1861 160 456 76 351 102 19
Cuts at: 4254 5045 6590 1132
Size: 791 1545 2229
Fragments arranged by size:
2229 1861 1545 791 456 351 160 102
97 76 19
PasI CC'CwG_GG
Cuts at: 1348 4706 1348
Size: 3358 4329
PciI A'CATG_T
Cuts at: 6586 6586
Size: 7687
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 3419 1108
Size: 1287 1024 5376
Fragments arranged by size:
5376 1287 1024
PfoI T'CCnGG_A
Cuts at: 3614 4569 3614
Size: 955 6732
PmeI GTTT'AAAC
Cuts at: 4991 4991
Size: 7687
PmlI CAC'GTG
Cuts at: 4120 4120
Size: 7687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5756 5788 7269 7301 5756
Size: 32 1481 32 6142
Fragments arranged by size:
6142 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2304 2827 4827 2304
Size: 523 2000 5164
Fragments arranged by size:
5164 2000 523
PshAI GACnn'nnGTC
Cuts at: 1613 2820 4457 1613
Size: 1207 1637 4843
Fragments arranged by size:
4843 1637 1207
PsiI TTA'TAA
Cuts at: 5257 5257
Size: 7687
PspOMI G'GGCC_C
Cuts at: 2049 2849 4836 2049
Size: 800 1987 4900
Fragments arranged by size:
4900 1987 800
PspXI vC'TCGA_Gb
Cuts at: 4588 4588
Size: 7687
PstI C_TGCA'G
Cuts at: 830 2439 2639 3746 4029 5039 830
Size: 1609 200 1107 283 1010 3478
Fragments arranged by size:
3478 1609 1107 1010 283 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7687
PvuII CAG'CTG
Cuts at: 2112 2112
Size: 7687
SalI G'TCGA_C
Cuts at: 1936 5029 1936
Size: 3093 4594
SanDI GG'GwC_CC
Cuts at: 2304 2304
Size: 7687
SapI GCTCTTCn'nnn_
Cuts at: 2354 2565 2354
Size: 211 7476
SbfI CC_TGCA'GG
Cuts at: 5039 5039
Size: 7687
ScaI AGT'ACT
Cuts at: 5935 7647 5935
Size: 1712 5975
SfcI C'TryA_G
Cuts at: 826 1045 2125 2435 2538 2635 3352 3710 3742
Size: 219 1080 310 103 97 717 358 32
Cuts at: 3742 4025 5035 6170 6851 7042 826
Size: 283 1010 1135 681 191 1471
Fragments arranged by size:
1471 1135 1080 1010 717 681 358 310
283 219 191 103 97 32
SfoI GGC'GCC
Cuts at: 2015 2015
Size: 7687
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7687
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7687
SmaI CCC'GGG
Cuts at: 1872 2048 2054 2149 5014 1872
Size: 176 6 95 2865 4545
Fragments arranged by size:
4545 2865 176 95 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4552 4588 5791 6692 6954 820
Size: 197 926 2609 36 1203 901 262 1553
Fragments arranged by size:
2609 1553 1203 926 901 262 197 36
SnaBI TAC'GTA
Cuts at: 493 2431 5478 493
Size: 1938 3047 2702
Fragments arranged by size:
3047 2702 1938
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7687
SphI G_CATG'C
Cuts at: 1132 4133 5045 1132
Size: 3001 912 3774
Fragments arranged by size:
3774 3001 912
SrfI GCCC'GGGC
Cuts at: 2048 2048
Size: 7687
SspI AAT'ATT
Cuts at: 5 52 5611 5
Size: 47 5559 2081
Fragments arranged by size:
5559 2081 47
StuI AGG'CCT
Cuts at: 1517 3358 3740 1517
Size: 1841 382 5464
Fragments arranged by size:
5464 1841 382
StyI C'CwwG_G
Cuts at: 513 1065 2394 2845 3052 3941 5126 513
Size: 552 1329 451 207 889 1185 3074
Fragments arranged by size:
3074 1329 1185 889 552 451 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2182 2972 5714 5731 5884 6069 6514
Size: 1255 602 790 2742 17 153 185 445
Cuts at: 6514 325
Size: 1498
Fragments arranged by size:
2742 1498 1255 790 602 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2114 3781 5933
Size: 274 80 33 51 1443 137 1667 2152
Cuts at: 5933 7645 96
Size: 1712 138
Fragments arranged by size:
2152 1712 1667 1443 274 138 137 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7647 7679 7647
Size: 32 7655
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7687
XbaI T'CTAG_A
Cuts at: 5023 5023
Size: 7687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 4075 2183
Size: 1892 5795
XhoI C'TCGA_G
Cuts at: 1943 4588 1943
Size: 2645 5042
XmaI C'CCGG_G
Cuts at: 1870 2046 2052 2147 5012 1870
Size: 176 6 95 2865 4545
Fragments arranged by size:
4545 2865 176 95 6
XmnI GAAnn'nnTTC
Cuts at: 3098 3806 5816 3098
Size: 708 2010 4969
Fragments arranged by size:
4969 2010 708
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3342 5495 276
Size: 53 83 186 936 1808 2153 2468
Fragments arranged by size:
2468 2153 1808 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsiWI
DraIII EcoICRI FseI FspAI NruI PacI PsrI RsrII
SacI SacII SexAI SfiI SwaI