Restriction map for
pFN21AE3764
(Circular) (Six-base) MAPSORT of: pFN21AE3764.seq Check: 573 from: 1 to: 6595
pFN21AE3764 6595 bp
With 182 enzymes: SgfI *
March 28, 2012 12:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2211 2211
Size: 6595
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2994 4405 278
Size: 53 83 186 936 1458 1411 2468
Fragments arranged by size:
2468 1458 1411 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3938 1937
Size: 2001 4594
Acc65I G'GTAC_C
Cuts at: 2333 3916 2333
Size: 1583 5012
AclI AA'CG_TT
Cuts at: 1563 4722 5095 1563
Size: 3159 373 3063
Fragments arranged by size:
3159 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2375 2753 3753 4658 6042 6310 1632
Size: 600 143 378 1000 905 1384 268 1917
Fragments arranged by size:
1917 1384 1000 905 600 378 268 143
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6398
AflIII A'CryG_T
Cuts at: 1203 3363 5494 6471 1203
Size: 2160 2131 977 1327
Fragments arranged by size:
2160 2131 1327 977
AgeI A'CCGG_T
Cuts at: 5426 5426
Size: 6595
AhdI GACnn_n'nnGTC
Cuts at: 5324 5324
Size: 6595
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6595
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6595
AlwNI CAG_nnn'CTG
Cuts at: 2452 2557 3500 3565 4338 5910 2452
Size: 105 943 65 773 1572 3137
Fragments arranged by size:
3137 1572 943 773 105 65
ApaLI G'TGCA_C
Cuts at: 2254 4652 5808 2254
Size: 2398 1156 3041
Fragments arranged by size:
3041 2398 1156
ApoI r'AATT_y
Cuts at: 1493 3135 3904 4130 4282 6391 1493
Size: 1642 769 226 152 2109 1697
Fragments arranged by size:
2109 1697 1642 769 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3402 5149 160
Size: 872 2370 1747 1606
Fragments arranged by size:
2370 1747 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2693 3920 1870
Size: 73 126 624 1227 4545
Fragments arranged by size:
4545 1227 624 126 73
BamHI G'GATC_C
Cuts at: 2893 3727 3925 4292 2893
Size: 834 198 367 5196
Fragments arranged by size:
5196 834 367 198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 2194 2333 2344 3490
Size: 325 217 627 277 130 139 11 1146
Cuts at: 3490 3512 3916 5371 618
Size: 22 404 1455 1842
Fragments arranged by size:
1842 1455 1146 627 404 325 277 217
139 130 22 11
BanII G_rGCy'C
Cuts at: 1391 2168 2243 3473 3914 1391
Size: 777 75 1230 441 4072
Fragments arranged by size:
4072 1230 777 441 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4786 4820 1903
Size: 34 50 34 2765 34 3678
Fragments arranged by size:
3678 2765 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4487 5697 4487
Size: 1210 5385
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6595
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2297 5206 136
Size: 107 122 71 631 605 625 2909 1525
Fragments arranged by size:
2909 1525 631 625 605 122 107 71
BglII A'GATC_T
Cuts at: 6590 6590
Size: 6595
BlpI GC'TnA_GC
Cuts at: 3596 4012 3596
Size: 416 6179
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2258 2495 4656 5812 1790
Size: 277 191 237 2161 1156 2573
Fragments arranged by size:
2573 2161 1156 277 237 191
BmgBI CAC'GTC
Cuts at: 1318 2121 1318
Size: 803 5792
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3473 4326 5284
Size: 416 53 83 399 24 2056 853 958
Cuts at: 5284 442
Size: 1753
Fragments arranged by size:
2056 1753 958 853 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6595
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3395
Size: 6595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2712 2907 5255 1239
Size: 1106 367 195 2348 2579
Fragments arranged by size:
2579 2348 1106 367 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4720 5585 5883 4720
Size: 865 298 5432
Fragments arranged by size:
5432 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5258 882
Size: 617 3759 2219
Fragments arranged by size:
3759 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2923 3366 4386 493
Size: 711 1719 443 1020 2702
Fragments arranged by size:
2702 1719 1020 711 443
BsaBI GATnn'nnATC
Cuts at: 3867 6589 3867
Size: 2722 3873
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2991 4402 4784 5419
Size: 53 83 186 936 1458 1411 382 635
Cuts at: 5419 275
Size: 1451
Fragments arranged by size:
1458 1451 1411 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2141 3024 5028 5426 5700 5847 6258 2141
Size: 883 2004 398 274 147 411 2478
Fragments arranged by size:
2478 2004 883 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2136 2166 2419 2449 3702
Size: 30 165 30 733 30 253 30 1253
Cuts at: 3702 3732 1178
Size: 30 4041
Fragments arranged by size:
4041 1253 733 253 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3092 3119 3811 1186
Size: 1906 27 692 3970
Fragments arranged by size:
3970 1906 692 27
BseYI C'CCAG_C
Cuts at: 3336 3522 5798 6294 3336
Size: 186 2276 496 3637
Fragments arranged by size:
3637 2276 496 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 2415 3283 3350 1163
Size: 573 327 352 868 67 4408
Fragments arranged by size:
4408 868 573 352 327 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2154 4045 4806 5834 1540
Size: 275 186 153 1891 761 1028 2301
Fragments arranged by size:
2301 1891 1028 761 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 2258 4656 4741 5812 2258
Size: 2398 85 1071 3041
Fragments arranged by size:
3041 2398 1071 85
BsmI GAATG_Cn'
Cuts at: 2972 3584 3883 4106 4199 2972
Size: 612 299 223 93 5368
Fragments arranged by size:
5368 612 299 223 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2168 2243 2258 2495 3473 3914
Size: 399 277 101 75 15 237 978 441
Cuts at: 3914 4656 4741 5812 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 978 742 441 399 277 237
101 85 75 15
BspEI T'CCGG_A
Cuts at: 2141 6258 2141
Size: 4117 2478
BspHI T'CATG_A
Cuts at: 3118 4484 6214 3118
Size: 1366 1730 3499
Fragments arranged by size:
3499 1730 1366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2211 3950 844
Size: 1367 1739 3489
Fragments arranged by size:
3489 1739 1367
BsrBI CCG'CTC
Cuts at: 1394 1823 4482 1394
Size: 429 2659 3507
Fragments arranged by size:
3507 2659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5090 5264 65
Size: 1183 3842 174 1396
Fragments arranged by size:
3842 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5239 5426 1700
Size: 110 3429 187 2869
Fragments arranged by size:
3429 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6595
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6595
BssSI C'ACGA_G
Cuts at: 2039 4655 5667 2039
Size: 2616 1012 2967
Fragments arranged by size:
2967 2616 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6595
BstBI TT'CG_AA
Cuts at: 5399 5399
Size: 6595
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6595
BstYI r'GATC_y
Cuts at: 1972 2893 2932 3376 3727 3925 4292 4677 4694
Size: 921 39 444 351 198 367 385 17
Cuts at: 4694 6146 6232 6244 6590 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 921 444 385 367 351 346
198 86 39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3195 3473 513
Size: 552 2130 278 3635
Fragments arranged by size:
3635 2130 552 278
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5856
Fragments arranged by size:
5856 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 3478 4111 4905 4925 3274
Size: 204 633 794 20 4944
Fragments arranged by size:
4944 794 633 204 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6560
DraI TTT'AAA
Cuts at: 3899 3899
Size: 6595
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5602 809
Size: 507 4286 1802
Fragments arranged by size:
4286 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2289 2298
Size: 54 1425 50 275 18 187 272 9
Cuts at: 2298 3670 3775 4042 4931 6560 8
Size: 1372 105 267 889 1629 43
Fragments arranged by size:
1629 1425 1372 889 275 272 267 187
105 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4042 1537
Size: 275 2230 4090
Fragments arranged by size:
4090 2230 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2401 3420 4525 1353
Size: 710 338 1019 1105 3423
Fragments arranged by size:
3423 1105 1019 710 338
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5182 5556 5702 387
Size: 1064 138 3593 374 146 1280
Fragments arranged by size:
3593 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2345 2375 2712 2753 2907 3753
Size: 393 600 113 30 337 41 154 846
Cuts at: 3753 4658 5255 6042 6310 1239
Size: 905 597 787 268 1524
Fragments arranged by size:
1524 905 846 787 600 597 393 337
268 154 113 41 30
EcoO109I rG'GnC_Cy
Cuts at: 2374 2449 2374
Size: 75 6520
EcoRI G'AATT_C
Cuts at: 3904 4282 3904
Size: 378 6217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3857 3857
Size: 6595
FspI TGC'GCA
Cuts at: 5101 5101
Size: 6595
HaeII r_GCGC'y
Cuts at: 1678 5742 1678
Size: 4064 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2136 2168 2419 2451 3100
Size: 32 135 32 564 32 251 32 649
Cuts at: 3100 3132 3739 3771 5236 5268 5310 5342 1373
Size: 32 607 32 1465 32 42 32 2626
Fragments arranged by size:
2626 1465 649 607 564 251 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3939 4185 669
Size: 988 281 2001 246 3079
Fragments arranged by size:
3079 2001 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6595
HpaI GTT'AAC
Cuts at: 4185 4185
Size: 6595
KpnI G_GTAC'C
Cuts at: 2337 3920 2337
Size: 1583 5012
MfeI C'AATT_G
Cuts at: 4194 4194
Size: 6595
MluI A'CGCG_T
Cuts at: 6471 6471
Size: 6595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3912 5709 5893 1305
Size: 2607 1797 184 2007
Fragments arranged by size:
2607 2007 1797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 3672 10
Size: 54 1425 343 459 1381 2933
Fragments arranged by size:
2933 1425 1381 459 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 4553 4912 5071 518
Size: 648 1656 1731 359 159 2042
Fragments arranged by size:
2042 1731 1656 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2388 3565 4011 4688 5836 6081 1606
Size: 782 1177 446 677 1148 245 2120
Fragments arranged by size:
2120 1177 1148 782 677 446 245
NcoI C'CATG_G
Cuts at: 513 1065 3473 513
Size: 552 2408 3635
Fragments arranged by size:
3635 2408 552
NdeI CA'TA_TG
Cuts at: 387 2862 3795 387
Size: 2475 933 3187
Fragments arranged by size:
3187 2475 933
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6595
NotI GC'GGCC_GC
Cuts at: 4042 4042
Size: 6595
NspI r_CATG'y
Cuts at: 1132 1229 2811 3953 5498 1132
Size: 97 1582 1142 1545 2229
Fragments arranged by size:
2229 1582 1545 1142 97
PasI CC'CwG_GG
Cuts at: 1348 2507 1348
Size: 1159 5436
PciI A'CATG_T
Cuts at: 5494 5494
Size: 6595
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3161 3463 1108
Size: 2053 302 4240
Fragments arranged by size:
4240 2053 302
PfoI T'CCnGG_A
Cuts at: 2361 2423 2361
Size: 62 6533
PmeI GTTT'AAAC
Cuts at: 3899 3899
Size: 6595
PmlI CAC'GTG
Cuts at: 3366 3366
Size: 6595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2150 2182 4664 4696 6177 6209 2150
Size: 32 2482 32 1481 32 2536
Fragments arranged by size:
2536 2482 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 2449 2374
Size: 75 6520
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6595
PsiI TTA'TAA
Cuts at: 4165 4165
Size: 6595
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2638 2670 2638
Size: 32 6563
PstI C_TGCA'G
Cuts at: 830 3947 830
Size: 3117 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6595
PvuII CAG'CTG
Cuts at: 3565 3565
Size: 6595
SalI G'TCGA_C
Cuts at: 1936 3937 1936
Size: 2001 4594
SanDI GG'GwC_CC
Cuts at: 2374 2374
Size: 6595
SapI GCTCTTCn'nnn_
Cuts at: 3420 3420
Size: 6595
SbfI CC_TGCA'GG
Cuts at: 3947 3947
Size: 6595
ScaI AGT'ACT
Cuts at: 3038 4843 6555 3038
Size: 1805 1712 3078
Fragments arranged by size:
3078 1805 1712
SexAI A'CCwGG_T
Cuts at: 2451 2451
Size: 6595
SfcI C'TryA_G
Cuts at: 826 1045 2549 2904 3692 3943 5078 5759 5950
Size: 219 1504 355 788 251 1135 681 191
Cuts at: 5950 826
Size: 1471
Fragments arranged by size:
1504 1471 1135 788 681 355 251 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2297 2297
Size: 6595
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6595
SmaI CCC'GGG
Cuts at: 1872 2071 2695 3922 1872
Size: 199 624 1227 4545
Fragments arranged by size:
4545 1227 624 199
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4699 5600 5862 820
Size: 197 926 2756 901 262 1553
Fragments arranged by size:
2756 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2923 4386 493
Size: 2430 1463 2702
Fragments arranged by size:
2702 2430 1463
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6595
SphI G_CATG'C
Cuts at: 1132 3953 1132
Size: 2821 3774
SspI AAT'ATT
Cuts at: 5 52 3125 4519 5
Size: 47 3073 1394 2081
Fragments arranged by size:
3073 2081 1394 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6595
StyI C'CwwG_G
Cuts at: 513 1065 2124 2752 3473 3750 4034 513
Size: 552 1059 628 721 277 284 3074
Fragments arranged by size:
3074 1059 721 628 552 284 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 4622 4639 4792 4977 5422 325
Size: 1255 560 2482 17 153 185 445 1498
Fragments arranged by size:
2482 1498 1255 560 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 3036 3422
Size: 274 80 33 51 1443 114 945 386
Cuts at: 3422 4841 6553 96
Size: 1419 1712 138
Fragments arranged by size:
1712 1443 1419 945 386 274 138 114
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6555 6587 6555
Size: 32 6563
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6595
XbaI T'CTAG_A
Cuts at: 3931 3931
Size: 6595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3202 3202
Size: 6595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6595
XmaI C'CCGG_G
Cuts at: 1870 2069 2693 3920 1870
Size: 199 624 1227 4545
Fragments arranged by size:
4545 1227 624 199
XmnI GAAnn'nnTTC
Cuts at: 3501 4724 3501
Size: 1223 5372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2992 4403 276
Size: 53 83 186 936 1458 1411 2468
Fragments arranged by size:
2468 1458 1411 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI
SacII SfoI SrfI SwaI