Restriction map for
pFN21AE3763
(Circular) (Six-base) MAPSORT of: pFN21AE3763.seq Check: 8576 from: 1 to: 6382
pFN21AE3763 6382 bp
With 182 enzymes: SgfI *
March 28, 2012 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2776 2776
Size: 6382
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3250 3382 3725 1937
Size: 1313 132 343 4594
Fragments arranged by size:
4594 1313 343 132
Acc65I G'GTAC_C
Cuts at: 3703 3703
Size: 6382
AclI AA'CG_TT
Cuts at: 1563 4509 4882 1563
Size: 2946 373 3063
Fragments arranged by size:
3063 2946 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4445 5829 6097 1632
Size: 2813 1384 268 1917
Fragments arranged by size:
2813 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6185
AflIII A'CryG_T
Cuts at: 1203 5281 6258 1203
Size: 4078 977 1327
Fragments arranged by size:
4078 1327 977
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 5111 5111
Size: 6382
AleI CACnn'nnGTG
Cuts at: 1166 3204 1166
Size: 2038 4344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6382
AlwNI CAG_nnn'CTG
Cuts at: 2022 3467 3533 4125 5697 2022
Size: 1445 66 592 1572 2707
Fragments arranged by size:
2707 1572 1445 592 66
ApaLI G'TGCA_C
Cuts at: 4439 5595 4439
Size: 1156 5226
ApoI r'AATT_y
Cuts at: 1493 2229 2540 3638 3669 3691 3917 4069 6178
Size: 736 311 1098 31 22 226 152 2109
Cuts at: 6178 1493
Size: 1697
Fragments arranged by size:
2109 1697 1098 736 311 226 152 31
22
AseI AT'TA_AT
Cuts at: 160 1032 4936 160
Size: 872 3904 1606
Fragments arranged by size:
3904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 3707 1870
Size: 73 1764 4545
Fragments arranged by size:
4545 1764 73
BamHI G'GATC_C
Cuts at: 2791 2875 2989 3712 4079 2791
Size: 84 114 723 367 5094
Fragments arranged by size:
5094 723 367 114 84
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 3703 5158 618
Size: 325 217 627 324 1592 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 324 217
BanII G_rGCy'C
Cuts at: 1391 3701 1391
Size: 2310 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5411
BbvCI CC'TCA_GC
Cuts at: 3534 3534
Size: 6382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4573 4607 1903
Size: 34 50 34 2552 34 3678
Fragments arranged by size:
3678 2552 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4274 5484 4274
Size: 1210 5172
BclI T'GATC_A
Cuts at: 1548 3094 1548
Size: 1546 4836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4993 136
Size: 107 122 71 631 605 3321 1525
Fragments arranged by size:
3321 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6377 6377
Size: 6382
BlpI GC'TnA_GC
Cuts at: 3799 3799
Size: 6382
Bme1580I G_kGCm'C
Cuts at: 1790 3160 4443 5599 1790
Size: 1370 1283 1156 2573
Fragments arranged by size:
2573 1370 1283 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6382
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2160 4113 5071
Size: 416 53 83 399 24 743 1953 958
Cuts at: 5071 442
Size: 1753
Fragments arranged by size:
1953 1753 958 743 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 5042 1239
Size: 805 2998 2579
Fragments arranged by size:
2998 2579 805
Bpu10I CC'TnA_GC
Cuts at: 3534 3534
Size: 6382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 3525 4507 5372 5670 2225
Size: 1300 982 865 298 2937
Fragments arranged by size:
2937 1300 982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5045 882
Size: 617 3546 2219
Fragments arranged by size:
3546 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4173 493
Size: 711 2969 2702
Fragments arranged by size:
2969 2702 711
BsaBI GATnn'nnATC
Cuts at: 3096 3487 6376 3096
Size: 391 2889 3102
Fragments arranged by size:
3102 2889 391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2389 4189 4571 5206
Size: 53 83 186 936 856 1800 382 635
Cuts at: 5206 275
Size: 1451
Fragments arranged by size:
1800 1451 936 856 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4815 5213 5487 5634 6045 4815
Size: 398 274 147 411 5152
Fragments arranged by size:
5152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3457 3487 1178
Size: 30 165 30 2054 30 4073
Fragments arranged by size:
4073 2054 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2346 1186
Size: 1160 5222
BseYI C'CCAG_C
Cuts at: 2101 5585 6081 2101
Size: 3484 496 2402
Fragments arranged by size:
3484 2402 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2562 1163
Size: 573 826 4983
Fragments arranged by size:
4983 826 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3832 4593 5621 1540
Size: 275 186 1831 761 1028 2301
Fragments arranged by size:
2301 1831 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2945 4443 4528 5599 2945
Size: 1498 85 1071 3728
Fragments arranged by size:
3728 1498 1071 85
BsmI GAATG_Cn'
Cuts at: 2497 3893 3986 2497
Size: 1396 93 4893
Fragments arranged by size:
4893 1396 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2945 3160 3701 4443 4528 5599 1391
Size: 399 1155 215 541 742 85 1071 2174
Fragments arranged by size:
2174 1155 1071 742 541 399 215 85
BspEI T'CCGG_A
Cuts at: 6045 6045
Size: 6382
BspHI T'CATG_A
Cuts at: 4271 6001 4271
Size: 1730 4652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2776 3737 844
Size: 1932 961 3489
Fragments arranged by size:
3489 1932 961
BsrBI CCG'CTC
Cuts at: 1394 1823 4269 1394
Size: 429 2446 3507
Fragments arranged by size:
3507 2446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4877 5051 65
Size: 1183 3629 174 1396
Fragments arranged by size:
3629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5026 5213 1700
Size: 110 3216 187 2869
Fragments arranged by size:
3216 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6382
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 4442 5454 4442
Size: 1012 5370
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6382
BstBI TT'CG_AA
Cuts at: 5186 5186
Size: 6382
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6382
BstYI r'GATC_y
Cuts at: 1972 2403 2724 2791 2875 2989 3712 4079 4464
Size: 431 321 67 84 114 723 367 385
Cuts at: 4464 4481 5933 6019 6031 6377 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 723 431 385 367 346 321
114 86 84 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2871 2871
Size: 6382
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5830
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5643
Fragments arranged by size:
5643 683 56
BtsI GCAGTG_nn'
Cuts at: 2641 3898 4692 4712 2641
Size: 1257 794 20 4311
Fragments arranged by size:
4311 1257 794 20
ClaI AT'CG_AT
Cuts at: 1554 3505 1554
Size: 1951 4431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6347
DraI TTT'AAA
Cuts at: 2748 3686 2748
Size: 938 5444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3624 5389 809
Size: 507 2308 1765 1802
Fragments arranged by size:
2308 1802 1765 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 3829 4718
Size: 54 1425 50 275 18 247 1752 889
Cuts at: 4718 6347 8
Size: 1629 43
Fragments arranged by size:
1752 1629 1425 889 275 247 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3829 1537
Size: 275 2017 4090
Fragments arranged by size:
4090 2017 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2795 2943 3598 4312 1353
Size: 1442 148 655 714 3423
Fragments arranged by size:
3423 1442 714 655 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4969 5343 5489 387
Size: 1064 138 3380 374 146 1280
Fragments arranged by size:
3380 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 4445 5042 5829 6097 1239
Size: 393 412 2401 597 787 268 1524
Fragments arranged by size:
2401 1524 787 597 412 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2848 2848
Size: 6382
EcoRI G'AATT_C
Cuts at: 3691 4069 3691
Size: 378 6004
EcoRV GAT'ATC
Cuts at: 2821 2821
Size: 6382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3581 3581
Size: 6382
FspI TGC'GCA
Cuts at: 2009 4888 2009
Size: 2879 3503
HaeII r_GCGC'y
Cuts at: 1678 5529 1678
Size: 3851 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 2864 2896 3457
Size: 32 135 32 592 32 668 32 561
Cuts at: 3457 3489 5023 5055 5097 5129 1373
Size: 32 1534 32 42 32 2626
Fragments arranged by size:
2626 1534 668 592 561 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3251 3604 3726 3972 669
Size: 988 281 1313 353 122 246 3079
Fragments arranged by size:
3079 1313 988 353 281 246 122
HindIII A'AGCT_T
Cuts at: 748 2243 748
Size: 1495 4887
HpaI GTT'AAC
Cuts at: 3972 3972
Size: 6382
KpnI G_GTAC'C
Cuts at: 3707 3707
Size: 6382
MfeI C'AATT_G
Cuts at: 3293 3981 3293
Size: 688 5694
MluI A'CGCG_T
Cuts at: 6258 6258
Size: 6382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2422 5496 5680 1305
Size: 1117 3074 184 2007
Fragments arranged by size:
3074 2007 1117 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4560
Fragments arranged by size:
4560 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 2490 3204 4340 4699 4858 518
Size: 648 1146 178 714 1136 359 159 2042
Fragments arranged by size:
2042 1146 1136 714 648 359 178 159
MspA1I CmG'CkG
Cuts at: 1606 2964 3798 4475 5623 5868 1606
Size: 1358 834 677 1148 245 2120
Fragments arranged by size:
2120 1358 1148 834 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5830
NdeI CA'TA_TG
Cuts at: 387 2436 2778 387
Size: 2049 342 3991
Fragments arranged by size:
3991 2049 342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NspI r_CATG'y
Cuts at: 1132 1229 3740 5285 1132
Size: 97 2511 1545 2229
Fragments arranged by size:
2511 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6382
PciI A'CATG_T
Cuts at: 5281 5281
Size: 6382
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3620 1108
Size: 2512 3870
PfoI T'CCnGG_A
Cuts at: 2021 2021
Size: 6382
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4451 4483 5964 5996 4451
Size: 32 1481 32 4837
Fragments arranged by size:
4837 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2848 2848
Size: 6382
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6382
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PstI C_TGCA'G
Cuts at: 830 2787 3734 830
Size: 1957 947 3478
Fragments arranged by size:
3478 1957 947
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
PvuII CAG'CTG
Cuts at: 2964 2964
Size: 6382
SalI G'TCGA_C
Cuts at: 1936 3249 3724 1936
Size: 1313 475 4594
Fragments arranged by size:
4594 1313 475
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 4630 6342 4630
Size: 1712 4670
SexAI A'CCwGG_T
Cuts at: 2277 2277
Size: 6382
SfcI C'TryA_G
Cuts at: 826 1045 2783 3383 3730 4865 5546 5737 826
Size: 219 1738 600 347 1135 681 191 1471
Fragments arranged by size:
1738 1471 1135 681 600 347 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 3709 1872
Size: 1837 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 3540 4486 5387 5649 820
Size: 197 926 297 1300 946 901 262 1553
Fragments arranged by size:
1553 1300 946 926 901 297 262 197
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6382
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SspI AAT'ATT
Cuts at: 5 52 2744 4306 5
Size: 47 2692 1562 2081
Fragments arranged by size:
2692 2081 1562 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6382
StyI C'CwwG_G
Cuts at: 513 1065 3152 3202 3821 513
Size: 552 2087 50 619 3074
Fragments arranged by size:
3074 2087 619 552 50
SwaI ATTT'AAAT
Cuts at: 2748 2748
Size: 6382
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 2581 4409 4426 4579 4764 5209
Size: 1255 501 500 1828 17 153 185 445
Cuts at: 5209 325
Size: 1498
Fragments arranged by size:
1828 1498 1255 501 500 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2288 4628 6340
Size: 274 80 33 51 1443 311 2340 1712
Cuts at: 6340 96
Size: 138
Fragments arranged by size:
2340 1712 1443 311 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6342 6374 6342
Size: 32 6350
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6382
XbaI T'CTAG_A
Cuts at: 3718 3718
Size: 6382
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6382
XmaI C'CCGG_G
Cuts at: 1870 3707 1870
Size: 1837 4545
XmnI GAAnn'nnTTC
Cuts at: 4511 4511
Size: 6382
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI XcmI