Restriction map for
pFN21AE3754
(Circular) (Six-base) MAPSORT of: pFN21AE3754.seq Check: 2664 from: 1 to: 5452
pFN21AE3754 5452 bp
With 182 enzymes: SgfI *
March 28, 2012 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2366 2366
Size: 5452
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3262 278
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2529 2795 1937
Size: 592 266 4594
Fragments arranged by size:
4594 592 266
Acc65I G'GTAC_C
Cuts at: 2773 2773
Size: 5452
AclI AA'CG_TT
Cuts at: 1563 3579 3952 1563
Size: 2016 373 3063
Fragments arranged by size:
3063 2016 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2555 3515 4899 5167 1632
Size: 923 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 923 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5255
AflIII A'CryG_T
Cuts at: 1203 4351 5328 1203
Size: 3148 977 1327
Fragments arranged by size:
3148 1327 977
AgeI A'CCGG_T
Cuts at: 4283 4283
Size: 5452
AhdI GACnn_n'nnGTC
Cuts at: 4181 4181
Size: 5452
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2078 1184
Size: 894 4558
AlwNI CAG_nnn'CTG
Cuts at: 2597 3195 4767 2597
Size: 598 1572 3282
Fragments arranged by size:
3282 1572 598
ApaI G_GGCC'C
Cuts at: 2619 2701 2619
Size: 82 5370
ApaLI G'TGCA_C
Cuts at: 2286 2504 3509 4665 2286
Size: 218 1005 1156 3073
Fragments arranged by size:
3073 1156 1005 218
ApoI r'AATT_y
Cuts at: 1493 2585 2761 2987 3139 5248 1493
Size: 1092 176 226 152 2109 1697
Fragments arranged by size:
2109 1697 1092 226 176 152
AseI AT'TA_AT
Cuts at: 160 1032 4006 160
Size: 872 2974 1606
Fragments arranged by size:
2974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
AvaI C'yCGr_G
Cuts at: 1870 1943 2472 2612 2777 1870
Size: 73 529 140 165 4545
Fragments arranged by size:
4545 529 165 140 73
BamHI G'GATC_C
Cuts at: 2242 2679 2782 3149 2242
Size: 437 103 367 4545
Fragments arranged by size:
4545 437 367 103
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2054 2773 4228 618
Size: 325 217 627 250 17 719 1455 1842
Fragments arranged by size:
1842 1455 719 627 325 250 217 17
BanII G_rGCy'C
Cuts at: 1391 2314 2484 2619 2701 2771 1391
Size: 923 170 135 82 70 4072
Fragments arranged by size:
4072 923 170 135 82 70
BbeI G_GCGC'C
Cuts at: 2041 2041
Size: 5452
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3643 3677 1903
Size: 34 1706 34 3678
Fragments arranged by size:
3678 1706 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4554 3344
Size: 1210 4242
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2600 4063 136
Size: 107 122 71 631 605 928 1463 1525
Fragments arranged by size:
1525 1463 928 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5447 5447
Size: 5452
BlpI GC'TnA_GC
Cuts at: 2869 2869
Size: 5452
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2290 2508 2619 2701 3513 4669 1790
Size: 267 233 218 111 82 812 1156 2573
Fragments arranged by size:
2573 1156 812 267 233 218 111 82
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3183 4141 442
Size: 416 53 83 399 24 1766 958 1753
Fragments arranged by size:
1766 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5452
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2410 2600 4112 1239
Size: 1171 190 1512 2579
Fragments arranged by size:
2579 1512 1171 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 3577 4442 4740 2690
Size: 887 865 298 3402
Fragments arranged by size:
3402 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4115 882
Size: 617 2616 2219
Fragments arranged by size:
2616 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3243 493
Size: 711 2039 2702
Fragments arranged by size:
2702 2039 711
BsaBI GATnn'nnATC
Cuts at: 5446 5446
Size: 5452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2038 3259 3641 4276
Size: 53 83 186 936 505 1221 382 635
Cuts at: 4276 275
Size: 1451
Fragments arranged by size:
1451 1221 936 635 505 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3885 4283 4557 4704 5115 3885
Size: 398 274 147 411 4222
Fragments arranged by size:
4222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5227
Fragments arranged by size:
5227 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2207 1186
Size: 1006 15 4431
Fragments arranged by size:
4431 1006 15
BseYI C'CCAG_C
Cuts at: 2080 2400 2477 2619 4655 5151 2080
Size: 320 77 142 2036 496 2381
Fragments arranged by size:
2381 2036 496 320 142 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4879
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2902 3663 4691 1540
Size: 275 186 901 761 1028 2301
Fragments arranged by size:
2301 1028 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2290 2508 3513 3598 4669 2290
Size: 218 1005 85 1071 3073
Fragments arranged by size:
3073 1071 1005 218 85
BsmI GAATG_Cn'
Cuts at: 2963 3056 2963
Size: 93 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2290 2314 2484 2508 2619 2701
Size: 399 267 233 24 170 24 111 82
Cuts at: 2701 2771 3513 3598 4669 1391
Size: 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 267 233 170 111
85 82 70 24 24
BspEI T'CCGG_A
Cuts at: 5115 5115
Size: 5452
BspHI T'CATG_A
Cuts at: 3341 5071 3341
Size: 1730 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2366 2807 844
Size: 1522 441 3489
Fragments arranged by size:
3489 1522 441
BsrBI CCG'CTC
Cuts at: 1394 1823 2074 3339 1394
Size: 429 251 1265 3507
Fragments arranged by size:
3507 1265 429 251
BsrDI GCAATG_nn'
Cuts at: 65 1248 3947 4121 65
Size: 1183 2699 174 1396
Fragments arranged by size:
2699 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4096 4283 1700
Size: 110 2286 187 2869
Fragments arranged by size:
2869 2286 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5452
BssSI C'ACGA_G
Cuts at: 3512 4524 3512
Size: 1012 4440
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5452
BstBI TT'CG_AA
Cuts at: 4256 4256
Size: 5452
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5452
BstXI CCAn_nnnn'nTGG
Cuts at: 2642 2642
Size: 5452
BstYI r'GATC_y
Cuts at: 1972 2242 2679 2782 3149 3534 3551 5003 5089
Size: 270 437 103 367 385 17 1452 86
Cuts at: 5089 5101 5447 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 437 385 367 346 270 103
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 3841
Fragments arranged by size:
3841 939 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2133 487
Size: 683 56 907 3806
Fragments arranged by size:
3806 907 683 56
BtsI GCAGTG_nn'
Cuts at: 2595 2968 3762 3782 2595
Size: 373 794 20 4265
Fragments arranged by size:
4265 794 373 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5417
DraI TTT'AAA
Cuts at: 2756 2756
Size: 5452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4459 809
Size: 507 3143 1802
Fragments arranged by size:
3143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2121 2660
Size: 54 1425 50 275 18 177 114 539
Cuts at: 2660 2899 3788 5417 8
Size: 239 889 1629 43
Fragments arranged by size:
1629 1425 889 539 275 239 177 114
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2899 1537
Size: 275 1087 4090
Fragments arranged by size:
4090 1087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2311 2744 3382 1353
Size: 958 433 638 3423
Fragments arranged by size:
3423 958 638 433
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 2146 2615 4039 4413 4559
Size: 1064 138 455 102 469 1424 374 146
Cuts at: 4559 387
Size: 1280
Fragments arranged by size:
1424 1280 1064 469 455 374 146 138
102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 2555 2600 3515 4112 4899 5167
Size: 393 778 145 45 915 597 787 268
Cuts at: 5167 1239
Size: 1524
Fragments arranged by size:
1524 915 787 778 597 393 268 145
45
EcoNI CCTnn'n_nnAGG
Cuts at: 2633 2633
Size: 5452
EcoO109I rG'GnC_Cy
Cuts at: 2203 2616 2667 2203
Size: 413 51 4988
Fragments arranged by size:
4988 413 51
EcoRI G'AATT_C
Cuts at: 2585 2761 3139 2585
Size: 176 378 4898
Fragments arranged by size:
4898 378 176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 5452
FspI TGC'GCA
Cuts at: 3958 3958
Size: 5452
HaeII r_GCGC'y
Cuts at: 1678 2041 2099 4599 1678
Size: 363 58 2500 2531
Fragments arranged by size:
2531 2500 363 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4093 4125 4167 4199 1373
Size: 32 135 32 2521 32 42 32 2626
Fragments arranged by size:
2626 2521 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 3042 669
Size: 988 281 858 246 3079
Fragments arranged by size:
3079 988 858 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5452
HpaI GTT'AAC
Cuts at: 3042 3042
Size: 5452
KasI G'GCGC_C
Cuts at: 2037 2037
Size: 5452
KpnI G_GTAC'C
Cuts at: 2777 2777
Size: 5452
MfeI C'AATT_G
Cuts at: 3051 3051
Size: 5452
MluI A'CGCG_T
Cuts at: 5328 5328
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1997 2190 4566 4750 1305
Size: 692 193 2376 184 2007
Fragments arranged by size:
2376 2007 692 193 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3630
Fragments arranged by size:
3630 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2511 3410 3769 3928 518
Size: 648 1345 899 359 159 2042
Fragments arranged by size:
2042 1345 899 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2868 3545 4693 4938 1606
Size: 1262 677 1148 245 2120
Fragments arranged by size:
2120 1262 1148 677 245
NarI GG'CG_CC
Cuts at: 2038 2038
Size: 5452
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3961
Fragments arranged by size:
3961 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5452
NotI GC'GGCC_GC
Cuts at: 2899 2899
Size: 5452
NspI r_CATG'y
Cuts at: 1132 1229 2810 4355 1132
Size: 97 1581 1545 2229
Fragments arranged by size:
2229 1581 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2694 1348
Size: 1346 4106
PciI A'CATG_T
Cuts at: 4351 4351
Size: 5452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2354 1108
Size: 1246 4206
PmeI GTTT'AAAC
Cuts at: 2756 2756
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 5034 5066 3521
Size: 32 1481 32 3907
Fragments arranged by size:
3907 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 2667 2203
Size: 464 4988
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5452
PsiI TTA'TAA
Cuts at: 3022 3022
Size: 5452
PspOMI G'GGCC_C
Cuts at: 2615 2697 2615
Size: 82 5370
PstI C_TGCA'G
Cuts at: 830 2804 830
Size: 1974 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5452
SalI G'TCGA_C
Cuts at: 1936 2794 1936
Size: 858 4594
SapI GCTCTTCn'nnn_
Cuts at: 2311 2311
Size: 5452
SbfI CC_TGCA'GG
Cuts at: 2804 2804
Size: 5452
ScaI AGT'ACT
Cuts at: 3700 5412 3700
Size: 1712 3740
SfcI C'TryA_G
Cuts at: 826 1045 2800 3935 4616 4807 826
Size: 219 1755 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2039 2039
Size: 5452
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5452
SmaI CCC'GGG
Cuts at: 1872 2474 2779 1872
Size: 602 305 4545
Fragments arranged by size:
4545 602 305
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2705 3556 4457 4719 820
Size: 197 926 762 851 901 262 1553
Fragments arranged by size:
1553 926 901 851 762 262 197
SnaBI TAC'GTA
Cuts at: 493 3243 493
Size: 2750 2702
SpeI A'CTAG_T
Cuts at: 152 2266 152
Size: 2114 3338
SphI G_CATG'C
Cuts at: 1132 2810 1132
Size: 1678 3774
SspI AAT'ATT
Cuts at: 5 52 3376 5
Size: 47 3324 2081
Fragments arranged by size:
3324 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5452
StyI C'CwwG_G
Cuts at: 513 1065 2004 2891 513
Size: 552 939 887 3074
Fragments arranged by size:
3074 939 887 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3479 3496 3649 3834 4279 325
Size: 1255 1899 17 153 185 445 1498
Fragments arranged by size:
1899 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2493 3698 5410
Size: 274 80 33 51 1443 516 1205 1712
Cuts at: 5410 96
Size: 138
Fragments arranged by size:
1712 1443 1205 516 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 5412 5444 2380
Size: 32 3000 32 2388
Fragments arranged by size:
3000 2388 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5452
XbaI T'CTAG_A
Cuts at: 2788 2788
Size: 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5452
XmaI C'CCGG_G
Cuts at: 1870 2472 2777 1870
Size: 602 305 4545
Fragments arranged by size:
4545 602 305
XmnI GAAnn'nnTTC
Cuts at: 3581 3581
Size: 5452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3260 276
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI