Restriction map for
pFN21AE3719
(Circular) (Six-base) MAPSORT of: pFN21AE3719.seq Check: 763 from: 1 to: 10999
pFN21AE3719 10999 bp
With 182 enzymes: SgfI *
February 2, 2012 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2027 8809 278
Size: 53 83 186 936 491 6782 2468
Fragments arranged by size:
6782 2468 936 491 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6053 8342 1937
Size: 4116 2289 4594
Fragments arranged by size:
4594 4116 2289
Acc65I G'GTAC_C
Cuts at: 2965 8263 8320 2965
Size: 5298 57 5644
Fragments arranged by size:
5644 5298 57
AclI AA'CG_TT
Cuts at: 1563 9126 9499 1563
Size: 7563 373 3063
Fragments arranged by size:
7563 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 3468 4175 5127 5327 5653 5912 6218
Size: 669 1167 707 952 200 326 259 306
Cuts at: 6218 6263 6552 6644 6771 7104 7228 9062 10446
Size: 45 289 92 127 333 124 1834 1384
Cuts at: 10446 10714 1632
Size: 268 1917
Fragments arranged by size:
1917 1834 1384 1167 952 707 669 333
326 306 289 268 259 200 127 124
92 45
AflII C'TTAA_G
Cuts at: 820 1017 2656 820
Size: 197 1639 9163
Fragments arranged by size:
9163 1639 197
AflIII A'CryG_T
Cuts at: 1203 3610 4290 9898 10875 1203
Size: 2407 680 5608 977 1327
Fragments arranged by size:
5608 2407 1327 977 680
AgeI A'CCGG_T
Cuts at: 9830 9830
Size: 10999
AhdI GACnn_n'nnGTC
Cuts at: 3889 3956 9728 3889
Size: 67 5772 5160
Fragments arranged by size:
5772 5160 67
AleI CACnn'nnGTG
Cuts at: 1166 3102 1166
Size: 1936 9063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2904 4869 1184
Size: 1720 1965 7314
Fragments arranged by size:
7314 1965 1720
AlwNI CAG_nnn'CTG
Cuts at: 2720 5915 6241 6451 7595 8742 10314 2720
Size: 3195 326 210 1144 1147 1572 3405
Fragments arranged by size:
3405 3195 1572 1147 1144 326 210
ApaI G_GGCC'C
Cuts at: 6783 6783
Size: 10999
ApaLI G'TGCA_C
Cuts at: 9056 10212 9056
Size: 1156 9843
ApoI r'AATT_y
Cuts at: 1493 2513 2600 3045 3332 4113 4202 4430 4631
Size: 1020 87 445 287 781 89 228 201
Cuts at: 4631 5285 5487 5500 5738 6892 7740 8169 8308
Size: 654 202 13 238 1154 848 429 139
Cuts at: 8308 8534 8686 10795 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 1020 848 781 654 445
429 287 238 228 226 202 201 152
139 89 87 13
AseI AT'TA_AT
Cuts at: 160 1032 4323 4652 5643 9553 160
Size: 872 3291 329 991 3910 1606
Fragments arranged by size:
3910 3291 1606 991 872 329
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10999
AvaI C'yCGr_G
Cuts at: 1870 1943 4554 5235 7575 8324 1870
Size: 73 2611 681 2340 749 4545
Fragments arranged by size:
4545 2611 2340 749 681 73
AvrII C'CTAG_G
Cuts at: 4507 4507
Size: 10999
BamHI G'GATC_C
Cuts at: 4744 5023 6839 8329 8696 4744
Size: 279 1816 1490 367 7047
Fragments arranged by size:
7047 1816 1490 367 279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 6045 8263 8320 9775
Size: 325 217 627 1178 3080 2218 57 1455
Cuts at: 9775 618
Size: 1842
Fragments arranged by size:
3080 2218 1842 1455 1178 627 325 217
57
BanII G_rGCy'C
Cuts at: 1391 2124 3786 6078 6713 6783 7868 8318 1391
Size: 733 1662 2292 635 70 1085 450 4072
Fragments arranged by size:
4072 2292 1662 1085 733 635 450 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2703 6417 6595 928
Size: 971 804 3714 178 5332
Fragments arranged by size:
5332 3714 971 804 178
BbvCI CC'TCA_GC
Cuts at: 2235 2396 6444 2235
Size: 161 4048 6790
Fragments arranged by size:
6790 4048 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7625 7659 9190 9224 1903
Size: 34 5688 34 1531 34 3678
Fragments arranged by size:
5688 3678 1531 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8891 10101 8891
Size: 1210 9789
BclI T'GATC_A
Cuts at: 1548 2110 1548
Size: 562 10437
BfrBI ATG'CAT
Cuts at: 5531 6632 5531
Size: 1101 9898
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3212 9610 136
Size: 107 122 71 631 605 1540 6398 1525
Fragments arranged by size:
6398 1540 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2948 7801 10994 2948
Size: 4853 3193 2953
Fragments arranged by size:
4853 3193 2953
BlpI GC'TnA_GC
Cuts at: 5421 6072 8416 5421
Size: 651 2344 8004
Fragments arranged by size:
8004 2344 651
Bme1580I G_kGCm'C
Cuts at: 1790 3217 3618 4421 6048 6783 6981 9060 10216
Size: 1427 401 803 1627 735 198 2079 1156
Cuts at: 10216 1790
Size: 2573
Fragments arranged by size:
2573 2079 1627 1427 1156 803 735 401
198
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10999
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5171 5680 8730
Size: 416 53 83 399 24 3754 509 3050
Cuts at: 8730 9688 442
Size: 958 1753
Fragments arranged by size:
3754 3050 1753 958 509 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 3681 3800 3848 5892 6636 9659 1239
Size: 1049 1393 119 48 2044 744 3023 2579
Fragments arranged by size:
3023 2579 2044 1393 1049 744 119 48
Bpu10I CC'TnA_GC
Cuts at: 2235 2396 5324 6444 6786 2235
Size: 161 2928 1120 342 6448
Fragments arranged by size:
6448 2928 1120 342 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2402 7359 8165 9124 9989 10287 2402
Size: 4957 806 959 865 298 3114
Fragments arranged by size:
4957 3114 959 865 806 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2613 3017 4907 5046 5271 6556 7286
Size: 617 1114 404 1890 139 225 1285 730
Cuts at: 7286 9662 882
Size: 2376 2219
Fragments arranged by size:
2376 2219 1890 1285 1114 730 617 404
225 139
BsaAI yAC'GTr
Cuts at: 493 1204 7301 8790 493
Size: 711 6097 1489 2702
Fragments arranged by size:
6097 2702 1489 711
BsaBI GATnn'nnATC
Cuts at: 7935 10993 7935
Size: 3058 7941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 3344 6137 8806
Size: 53 83 186 936 491 1320 2793 2669
Cuts at: 8806 9188 9823 275
Size: 382 635 1451
Fragments arranged by size:
2793 2669 1451 1320 936 635 491 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4930 8209 9432 9830 10104 10251 10662 4930
Size: 3279 1223 398 274 147 411 5267
Fragments arranged by size:
5267 3279 1223 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3334 3364 1178
Size: 30 165 30 1931 30 8813
Fragments arranged by size:
8813 1931 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2844 3809 4545 5092 5259 5264 5493 7407
Size: 1658 965 736 547 167 5 229 1914
Cuts at: 7407 1186
Size: 4778
Fragments arranged by size:
4778 1914 1658 965 736 547 229 167
5
BseYI C'CCAG_C
Cuts at: 2891 6835 7004 10202 10698 2891
Size: 3944 169 3198 496 3192
Fragments arranged by size:
3944 3198 3192 496 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3083 5900 1163
Size: 573 1347 2817 6262
Fragments arranged by size:
6262 2817 1347 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8449 9210 10238 1540
Size: 275 186 6448 761 1028 2301
Fragments arranged by size:
6448 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3786 5411 5667 6713 9060 9145 10216 3786
Size: 1625 256 1046 2347 85 1071 4569
Fragments arranged by size:
4569 2347 1625 1071 1046 256 85
BsmI GAATG_Cn'
Cuts at: 2710 5460 8510 8603 2710
Size: 2750 3050 93 5106
Fragments arranged by size:
5106 3050 2750 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3335 3335
Size: 10999
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 3217 3618 3786 4421 5411 5667
Size: 399 334 1093 401 168 635 990 256
Cuts at: 5667 6048 6078 6713 6783 6981 7868 8318 9060
Size: 381 30 635 70 198 887 450 742
Cuts at: 9060 9145 10216 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1093 1071 990 887 742 635 635
450 401 399 381 334 256 198 168
85 70 30
BspEI T'CCGG_A
Cuts at: 8209 10662 8209
Size: 2453 8546
BspHI T'CATG_A
Cuts at: 2362 6097 8888 10618 2362
Size: 3735 2791 1730 2743
Fragments arranged by size:
3735 2791 2743 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5245 8354 844
Size: 4401 3109 3489
Fragments arranged by size:
4401 3489 3109
BsrBI CCG'CTC
Cuts at: 1394 1823 8886 1394
Size: 429 7063 3507
Fragments arranged by size:
7063 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2539 3246 4071 5701 6474 6981 7014
Size: 1183 1291 707 825 1630 773 507 33
Cuts at: 7014 8092 8247 9494 9668 65
Size: 1078 155 1247 174 1396
Fragments arranged by size:
1630 1396 1291 1247 1183 1078 825 773
707 507 174 155 33
BsrFI r'CCGG_y
Cuts at: 1700 1810 9643 9830 1700
Size: 110 7833 187 2869
Fragments arranged by size:
7833 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4514 8217 96
Size: 4418 3703 2878
Fragments arranged by size:
4418 3703 2878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10999
BssSI C'ACGA_G
Cuts at: 9059 10071 9059
Size: 1012 9987
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2574 7313 794
Size: 1780 4739 4480
Fragments arranged by size:
4739 4480 1780
BstBI TT'CG_AA
Cuts at: 2389 8119 9803 2389
Size: 5730 1684 3585
Fragments arranged by size:
5730 3585 1684
BstEII G'GTnAC_C
Cuts at: 1184 2265 1184
Size: 1081 9918
BstXI CCAn_nnnn'nTGG
Cuts at: 7732 8274 7732
Size: 542 10457
BstYI r'GATC_y
Cuts at: 1972 2948 4566 4744 5023 5329 6238 6839 7801
Size: 976 1618 178 279 306 909 601 962
Cuts at: 7801 8329 8696 9081 9098 10550 10636 10648 10994
Size: 528 367 385 17 1452 86 12 346
Cuts at: 10994 1972
Size: 1977
Fragments arranged by size:
1977 1618 1452 976 962 909 601 528
385 367 346 306 279 178 86 17
12
BstZ17I GTA'TAC
Cuts at: 6054 6054
Size: 10999
Bsu36I CC'TnA_GG
Cuts at: 3788 3788
Size: 10999
BtgI C'CryG_G
Cuts at: 513 1065 2004 3375 3816 5835 6638 7725 513
Size: 552 939 1371 441 2019 803 1087 3787
Fragments arranged by size:
3787 2019 1371 1087 939 803 552 441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6638 487
Size: 683 56 5412 4848
Fragments arranged by size:
5412 4848 683 56
BtsI GCAGTG_nn'
Cuts at: 3109 3317 7531 8515 9309 9329 3109
Size: 208 4214 984 794 20 4779
Fragments arranged by size:
4779 4214 984 794 208 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10999
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5604 5639 529
Size: 35 5040 35 5889
Fragments arranged by size:
5889 5040 35 35
DraI TTT'AAA
Cuts at: 2095 2131 3406 8303 2095
Size: 36 1275 4897 4791
Fragments arranged by size:
4897 4791 1275 36
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10006 809
Size: 507 8690 1802
Fragments arranged by size:
8690 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 5460 5548
Size: 54 1425 50 275 18 177 3453 88
Cuts at: 5548 5866 7734 8446 9335 10964 8
Size: 318 1868 712 889 1629 43
Fragments arranged by size:
3453 1868 1629 1425 889 712 318 275
177 88 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8446 1537
Size: 275 6634 4090
Fragments arranged by size:
6634 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2320 5574 5672 5683 7280 7282 8929 1353
Size: 967 3254 98 11 1597 2 1647 3423
Fragments arranged by size:
3423 3254 1647 1597 967 98 11 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9586 9960 10106 387
Size: 1064 138 7997 374 146 1280
Fragments arranged by size:
7997 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3784 6711 3784
Size: 2927 8072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2301 3468 3681 3800 3848 4175
Size: 393 656 13 1167 213 119 48 327
Cuts at: 4175 5127 5327 5653 5892 5912 6218 6263 6552
Size: 952 200 326 239 20 306 45 289
Cuts at: 6552 6636 6644 6771 7104 7228 9062 9659 10446
Size: 84 8 127 333 124 1834 597 787
Cuts at: 10446 10714 1239
Size: 268 1524
Fragments arranged by size:
1834 1524 1167 952 787 656 597 393
333 327 326 306 289 268 239 213
200 127 124 119 84 48 45 20
13 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3791 4682 6233 3791
Size: 891 1551 8557
Fragments arranged by size:
8557 1551 891
EcoO109I rG'GnC_Cy
Cuts at: 6211 6532 6779 6780 6211
Size: 321 247 1 10430
Fragments arranged by size:
10430 321 247 1
EcoRI G'AATT_C
Cuts at: 4202 5285 5487 8308 8686 4202
Size: 1083 202 2821 378 6515
Fragments arranged by size:
6515 2821 1083 378 202
EcoRV GAT'ATC
Cuts at: 3985 5921 3985
Size: 1936 9063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3705 6245 6268 7118 3705
Size: 2540 23 850 7586
Fragments arranged by size:
7586 2540 850 23
FspI TGC'GCA
Cuts at: 9505 9505
Size: 10999
HaeII r_GCGC'y
Cuts at: 1678 2314 5857 10146 1678
Size: 636 3543 4289 2531
Fragments arranged by size:
4289 3543 2531 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2095 2127 2218
Size: 32 135 32 437 32 54 32 91
Cuts at: 2218 2250 3130 3162 3334 3347 3366 3379 3518
Size: 32 880 32 172 13 19 13 139
Cuts at: 3518 3550 3657 3689 3716 3748 3875 3907 3942
Size: 32 107 32 27 32 127 32 35
Cuts at: 3942 3974 4719 4751 5987 6019 7049 7081 7342
Size: 32 745 32 1236 32 1030 32 261
Cuts at: 7342 7374 9640 9672 9714 9746 1373
Size: 32 2266 32 42 32 2626
Fragments arranged by size:
2626 2266 1236 1030 880 745 437 261
172 139 135 127 107 91 54 42
35 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 27 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 5616 6870 8343 8589 669
Size: 988 281 1240 2438 1254 1473 246 3079
Fragments arranged by size:
3079 2438 1473 1254 1240 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6510 8141 748
Size: 5762 1631 3606
Fragments arranged by size:
5762 3606 1631
HpaI GTT'AAC
Cuts at: 8589 8589
Size: 10999
KpnI G_GTAC'C
Cuts at: 2969 8267 8324 2969
Size: 5298 57 5644
Fragments arranged by size:
5644 5298 57
MfeI C'AATT_G
Cuts at: 3228 6734 8598 3228
Size: 3506 1864 5629
Fragments arranged by size:
5629 3506 1864
MluI A'CGCG_T
Cuts at: 10875 10875
Size: 10999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2204 3973 5880 6565 7130 8080 10113 10297
Size: 899 1769 1907 685 565 950 2033 184
Cuts at: 10297 1305
Size: 2007
Fragments arranged by size:
2033 2007 1907 1769 950 899 685 565
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 5550 5868 7736 10
Size: 54 1425 343 177 3541 318 1868 3273
Fragments arranged by size:
3541 3273 1868 1425 343 318 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3102 3914 4652 8128 8957 9316 9475
Size: 648 1936 812 738 3476 829 359 159
Cuts at: 9475 518
Size: 2042
Fragments arranged by size:
3476 2042 1936 829 812 738 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2308 2378 4396 5320 8415 9092 10240 10485
Size: 702 70 2018 924 3095 677 1148 245
Cuts at: 10485 1606
Size: 2120
Fragments arranged by size:
3095 2120 2018 1148 924 702 677 245
70
NcoI C'CATG_G
Cuts at: 513 1065 2004 3816 5835 513
Size: 552 939 1812 2019 5677
Fragments arranged by size:
5677 2019 1812 939 552
NdeI CA'TA_TG
Cuts at: 387 2974 3010 387
Size: 2587 36 8376
Fragments arranged by size:
8376 2587 36
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10999
NotI GC'GGCC_GC
Cuts at: 8446 8446
Size: 10999
NsiI A_TGCA'T
Cuts at: 5533 6634 5533
Size: 1101 9898
NspI r_CATG'y
Cuts at: 1132 1229 3614 4294 5821 8357 9902 1132
Size: 97 2385 680 1527 2536 1545 2229
Fragments arranged by size:
2536 2385 2229 1545 1527 680 97
PasI CC'CwG_GG
Cuts at: 1348 4740 1348
Size: 3392 7607
PciI A'CATG_T
Cuts at: 3610 4290 9898 3610
Size: 680 5608 4711
Fragments arranged by size:
5608 4711 680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3853 4740 5446 5915 6133 6584 1108
Size: 2745 887 706 469 218 451 5523
Fragments arranged by size:
5523 2745 887 706 469 451 218
PfoI T'CCnGG_A
Cuts at: 6583 6652 6901 6583
Size: 69 249 10681
Fragments arranged by size:
10681 249 69
PmeI GTTT'AAAC
Cuts at: 8303 8303
Size: 10999
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9068 9100 10581 10613 9068
Size: 32 1481 32 9454
Fragments arranged by size:
9454 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 6532 6532
Size: 10999
PshAI GACnn'nnGTC
Cuts at: 1613 2984 1613
Size: 1371 9628
PsiI TTA'TAA
Cuts at: 2280 7767 8569 2280
Size: 5487 802 4710
Fragments arranged by size:
5487 4710 802
PspOMI G'GGCC_C
Cuts at: 6779 6779
Size: 10999
PspXI vC'TCGA_Gb
Cuts at: 4554 4554
Size: 10999
PstI C_TGCA'G
Cuts at: 830 2570 3324 5022 6456 8351 830
Size: 1740 754 1698 1434 1895 3478
Fragments arranged by size:
3478 1895 1740 1698 1434 754
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10999
PvuII CAG'CTG
Cuts at: 2378 4396 5320 2378
Size: 2018 924 8057
Fragments arranged by size:
8057 2018 924
SacI G_AGCT'C
Cuts at: 3786 6713 3786
Size: 2927 8072
SalI G'TCGA_C
Cuts at: 1936 8341 1936
Size: 6405 4594
SapI GCTCTTCn'nnn_
Cuts at: 2320 5672 5683 2320
Size: 3352 11 7636
Fragments arranged by size:
7636 3352 11
SbfI CC_TGCA'GG
Cuts at: 8351 8351
Size: 10999
ScaI AGT'ACT
Cuts at: 5539 7117 9247 10959 5539
Size: 1578 2130 1712 5579
Fragments arranged by size:
5579 2130 1712 1578
SexAI A'CCwGG_T
Cuts at: 7323 7323
Size: 10999
SfcI C'TryA_G
Cuts at: 826 1045 2503 2566 3222 3320 4147 5018 5060
Size: 219 1458 63 656 98 827 871 42
Cuts at: 5060 6432 6452 8347 9482 10163 10354 826
Size: 1372 20 1895 1135 681 191 1471
Fragments arranged by size:
1895 1471 1458 1372 1135 871 827 681
656 219 191 98 63 42 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10999
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10999
SmaI CCC'GGG
Cuts at: 1872 7577 8326 1872
Size: 5705 749 4545
Fragments arranged by size:
5705 4545 749
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2417 2656 4554 7374 8144 9103
Size: 197 926 474 239 1898 2820 770 959
Cuts at: 9103 10004 10266 820
Size: 901 262 1553
Fragments arranged by size:
2820 1898 1553 959 926 901 770 474
262 239 197
SnaBI TAC'GTA
Cuts at: 493 8790 493
Size: 8297 2702
SpeI A'CTAG_T
Cuts at: 152 2069 152
Size: 1917 9082
SphI G_CATG'C
Cuts at: 1132 8357 1132
Size: 7225 3774
SspI AAT'ATT
Cuts at: 5 52 4575 5772 6195 6646 8923 5
Size: 47 4523 1197 423 451 2277 2081
Fragments arranged by size:
4523 2277 2081 1197 451 423 47
StuI AGG'CCT
Cuts at: 1517 5797 1517
Size: 4280 6719
StyI C'CwwG_G
Cuts at: 513 1065 2004 3816 4248 4366 4507 5720 5835
Size: 552 939 1812 432 118 141 1213 115
Cuts at: 5835 5869 8438 513
Size: 34 2569 3074
Fragments arranged by size:
3074 2569 1812 1213 939 552 432 141
118 115 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2284 6448 7367 9026 9043 9196 9381
Size: 1255 704 4164 919 1659 17 153 185
Cuts at: 9381 9826 325
Size: 445 1498
Fragments arranged by size:
4164 1659 1498 1255 919 704 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3326 4514 5537
Size: 274 80 33 51 1443 1349 1188 1023
Cuts at: 5537 7115 8217 9245 10957 96
Size: 1578 1102 1028 1712 138
Fragments arranged by size:
1712 1578 1443 1349 1188 1102 1028 1023
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10959 10991 10959
Size: 32 10967
Tth111I GACn'n_nGTC
Cuts at: 1316 2254 1316
Size: 938 10061
XbaI T'CTAG_A
Cuts at: 8335 8335
Size: 10999
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3101 3101
Size: 10999
XhoI C'TCGA_G
Cuts at: 1943 4554 1943
Size: 2611 8388
XmaI C'CCGG_G
Cuts at: 1870 7575 8324 1870
Size: 5705 749 4545
Fragments arranged by size:
5705 4545 749
XmnI GAAnn'nnTTC
Cuts at: 5199 6248 7101 7512 9128 5199
Size: 1049 853 411 1616 7070
Fragments arranged by size:
7070 1616 1049 853 411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2025 8807 276
Size: 53 83 186 936 491 6782 2468
Fragments arranged by size:
6782 2468 936 491 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BplI BsiWI
DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI