Restriction map for
pFN21AE3713
(Circular) (Six-base) MAPSORT of: pFN21AE3713.seq Check: 4564 from: 1 to: 10780
pFN21AE3713 10780 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8590 278
Size: 53 83 186 936 7054 2468
Fragments arranged by size:
7054 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4425 6764 8123 1937
Size: 2488 2339 1359 4594
Fragments arranged by size:
4594 2488 2339 1359
Acc65I G'GTAC_C
Cuts at: 8101 8101
Size: 10780
AclI AA'CG_TT
Cuts at: 1563 8907 9280 1563
Size: 7344 373 3063
Fragments arranged by size:
7344 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2906 3693 4171 5112 5537 5670 6743 7025
Size: 1274 787 478 941 425 133 1073 282
Cuts at: 7025 7233 7422 8843 10227 10495 1632
Size: 208 189 1421 1384 268 1917
Fragments arranged by size:
1917 1421 1384 1274 1073 941 787 478
425 282 268 208 189 133
AfeI AGC'GCT
Cuts at: 3529 7978 3529
Size: 4449 6331
AflII C'TTAA_G
Cuts at: 820 1017 4378 6557 820
Size: 197 3361 2179 5043
Fragments arranged by size:
5043 3361 2179 197
AflIII A'CryG_T
Cuts at: 1203 6015 6382 9679 10656 1203
Size: 4812 367 3297 977 1327
Fragments arranged by size:
4812 3297 1327 977 367
AgeI A'CCGG_T
Cuts at: 5896 9611 5896
Size: 3715 7065
AhdI GACnn_n'nnGTC
Cuts at: 7054 9509 7054
Size: 2455 8325
AleI CACnn'nnGTG
Cuts at: 1166 7760 1166
Size: 6594 4186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6411 1184
Size: 5227 5553
AlwNI CAG_nnn'CTG
Cuts at: 5004 5192 5213 5852 8059 8523 10095 5004
Size: 188 21 639 2207 464 1572 5689
Fragments arranged by size:
5689 2207 1572 639 464 188 21
ApaI G_GGCC'C
Cuts at: 2959 2959
Size: 10780
ApaLI G'TGCA_C
Cuts at: 8837 9993 8837
Size: 1156 9624
ApoI r'AATT_y
Cuts at: 1493 2096 2185 2192 2365 3284 3911 4061 4214
Size: 603 89 7 173 919 627 150 153
Cuts at: 4214 4461 4594 4979 5182 5240 5583 5642 5731
Size: 247 133 385 203 58 343 59 89
Cuts at: 5731 6322 6477 8089 8315 8467 10576 1493
Size: 591 155 1612 226 152 2109 1697
Fragments arranged by size:
2109 1697 1612 919 627 603 591 385
343 247 226 203 173 155 153 152
150 133 89 89 59 58 7
AseI AT'TA_AT
Cuts at: 160 1032 3198 9334 160
Size: 872 2166 6136 1606
Fragments arranged by size:
6136 2166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10780
AvaI C'yCGr_G
Cuts at: 1870 1943 7229 8105 1870
Size: 73 5286 876 4545
Fragments arranged by size:
5286 4545 876 73
AvrII C'CTAG_G
Cuts at: 3125 3125
Size: 10780
BamHI G'GATC_C
Cuts at: 3085 4722 7321 8110 8477 3085
Size: 1637 2599 789 367 5388
Fragments arranged by size:
5388 2599 1637 789 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6106 8101 9556 618
Size: 325 217 627 4319 1995 1455 1842
Fragments arranged by size:
4319 1995 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2821 2959 4707 5932 6233 7746 7784 8099
Size: 1430 138 1748 1225 301 1513 38 315
Cuts at: 8099 1391
Size: 4072
Fragments arranged by size:
4072 1748 1513 1430 1225 315 301 138
38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2643 3063 3821 4311 4572 5172 7239
Size: 971 744 420 758 490 261 600 2067
Cuts at: 7239 928
Size: 4469
Fragments arranged by size:
4469 2067 971 758 744 600 490 420
261
BbvCI CC'TCA_GC
Cuts at: 2019 2019
Size: 10780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8971 9005 1903
Size: 34 7034 34 3678
Fragments arranged by size:
7034 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5785 5945 6940 8672 9882 5785
Size: 160 995 1732 1210 6683
Fragments arranged by size:
6683 1732 1210 995 160
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10780
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9391 136
Size: 107 122 71 631 605 7719 1525
Fragments arranged by size:
7719 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6621 10775 6621
Size: 4154 6626
BlpI GC'TnA_GC
Cuts at: 8197 8197
Size: 10780
Bme1580I G_kGCm'C
Cuts at: 1790 2959 7932 8841 9997 1790
Size: 1169 4973 909 1156 2573
Fragments arranged by size:
4973 2573 1169 1156 909
BmgBI CAC'GTC
Cuts at: 1318 7291 7499 1318
Size: 5973 208 4599
Fragments arranged by size:
5973 4599 208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2962 3708 6028
Size: 416 53 83 399 24 1545 746 2320
Cuts at: 6028 7868 8037 8511 9469 442
Size: 1840 169 474 958 1753
Fragments arranged by size:
2320 1840 1753 1545 958 746 474 416
399 169 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10780
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7765 7765
Size: 10780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3855 3921 7476 9440 1239
Size: 2616 66 3555 1964 2579
Fragments arranged by size:
3555 2616 2579 1964 66
Bpu10I CC'TnA_GC
Cuts at: 2019 2019
Size: 10780
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2408 5522 5924 6245 8905 9770 10068 2408
Size: 3114 402 321 2660 865 298 3120
Fragments arranged by size:
3120 3114 2660 865 402 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5830 6566 9443 882
Size: 617 4331 736 2877 2219
Fragments arranged by size:
4331 2877 2219 736 617
BsaAI yAC'GTr
Cuts at: 493 1204 2071 2351 5961 6385 8571 493
Size: 711 867 280 3610 424 2186 2702
Fragments arranged by size:
3610 2702 2186 867 711 424 280
BsaBI GATnn'nnATC
Cuts at: 4181 10774 4181
Size: 6593 4187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2337 8587 8969 9604
Size: 53 83 186 936 804 6250 382 635
Cuts at: 9604 275
Size: 1451
Fragments arranged by size:
6250 1451 936 804 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5896 9213 9611 9885 10032 10443 5896
Size: 3317 398 274 147 411 6233
Fragments arranged by size:
6233 3317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3401 3431 1178
Size: 30 165 30 1998 30 8527
Fragments arranged by size:
8527 1998 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4385 1186
Size: 3199 7581
BseYI C'CCAG_C
Cuts at: 2938 4954 7012 7745 9983 10479 2938
Size: 2016 2058 733 2238 496 3239
Fragments arranged by size:
3239 2238 2058 2016 733 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3423 3516 6384 6407 7205 1163
Size: 573 1687 93 2868 23 798 4738
Fragments arranged by size:
4738 2868 1687 798 573 93 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8230 8991 10019 1540
Size: 275 186 6229 761 1028 2301
Fragments arranged by size:
6229 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2821 4562 6315 6501 7554 7920 8841 8926 9997
Size: 1741 1753 186 1053 366 921 85 1071
Cuts at: 9997 2821
Size: 3604
Fragments arranged by size:
3604 1753 1741 1071 1053 921 366 186
85
BsmI GAATG_Cn'
Cuts at: 3929 6181 8291 8384 3929
Size: 2252 2110 93 6325
Fragments arranged by size:
6325 2252 2110 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2821 2959 4562 4707 5932 6233 6315
Size: 399 1031 138 1603 145 1225 301 82
Cuts at: 6315 6501 7554 7746 7784 7920 7932 8099 8841
Size: 186 1053 192 38 136 12 167 742
Cuts at: 8841 8926 9997 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1603 1225 1071 1053 1031 742 399
301 192 186 167 145 138 136 85
82 38 12
BspEI T'CCGG_A
Cuts at: 10443 10443
Size: 10780
BspHI T'CATG_A
Cuts at: 4812 8669 10399 4812
Size: 3857 1730 5193
Fragments arranged by size:
5193 3857 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 8135 844
Size: 7291 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 8667 1394
Size: 429 6844 3507
Fragments arranged by size:
6844 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2113 2502 4958 9275 9449 65
Size: 1183 865 389 2456 4317 174 1396
Fragments arranged by size:
4317 2456 1396 1183 865 389 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5896 9424 9611 1700
Size: 110 4086 3528 187 2869
Fragments arranged by size:
4086 3528 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10780
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10780
BssSI C'ACGA_G
Cuts at: 3853 7886 8840 9852 3853
Size: 4033 954 1012 4781
Fragments arranged by size:
4781 4033 1012 954
BstAPI GCAn_nnn'nTGC
Cuts at: 794 7862 794
Size: 7068 3712
BstBI TT'CG_AA
Cuts at: 5244 9584 5244
Size: 4340 6440
BstEII G'GTnAC_C
Cuts at: 1184 2810 6194 1184
Size: 1626 3384 5770
Fragments arranged by size:
5770 3384 1626
BstXI CCAn_nnnn'nTGG
Cuts at: 5925 6583 5925
Size: 658 10122
BstYI r'GATC_y
Cuts at: 1972 3016 3085 3565 3987 4326 4722 6219 6621
Size: 1044 69 480 422 339 396 1497 402
Cuts at: 6621 7321 8110 8477 8862 8879 10331 10417 10429
Size: 700 789 367 385 17 1452 86 12
Cuts at: 10429 10775 1972
Size: 346 1977
Fragments arranged by size:
1977 1497 1452 1044 789 700 480 422
402 396 385 367 346 339 86 69
17 12
BstZ17I GTA'TAC
Cuts at: 6765 6765
Size: 10780
Bsu36I CC'TnA_GG
Cuts at: 3003 3660 3003
Size: 657 10123
BtgI C'CryG_G
Cuts at: 513 1065 2004 3973 513
Size: 552 939 1969 7320
Fragments arranged by size:
7320 1969 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2265 2547 487
Size: 683 56 1039 282 8720
Fragments arranged by size:
8720 1039 683 282 56
BtsI GCAGTG_nn'
Cuts at: 4198 4617 5214 6256 7964 8296 9090 9110 4198
Size: 419 597 1042 1708 332 794 20 5868
Fragments arranged by size:
5868 1708 1042 794 597 419 332 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10780
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10745
DraI TTT'AAA
Cuts at: 5460 5647 8084 5460
Size: 187 2437 8156
Fragments arranged by size:
8156 2437 187
DraIII CAC_nnn'GTG
Cuts at: 2861 7862 7891 2861
Size: 5001 29 5750
Fragments arranged by size:
5750 5001 29
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8017 9787 809
Size: 507 6701 1770 1802
Fragments arranged by size:
6701 1802 1770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2276 2897 3210
Size: 54 1425 50 275 18 446 621 313
Cuts at: 3210 3594 6060 7261 7435 8227 9116 10745 8
Size: 384 2466 1201 174 792 889 1629 43
Fragments arranged by size:
2466 1629 1425 1201 889 792 621 446
384 313 275 174 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8227 1537
Size: 275 6415 4090
Fragments arranged by size:
6415 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2718 3867 4313 4401 5833 6564 6582 6858
Size: 1365 1149 446 88 1432 731 18 276
Cuts at: 6858 6891 8010 8026 8077 8710 1353
Size: 33 1119 16 51 633 3423
Fragments arranged by size:
3423 1432 1365 1149 1119 731 633 446
276 88 51 33 18 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 3257 9367 9741 9887 387
Size: 1064 138 465 1203 6110 374 146 1280
Fragments arranged by size:
6110 1280 1203 1064 465 374 146 138
EcoICRI GAG'CTC
Cuts at: 2819 2819
Size: 10780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2906 3693 3855 3921 4171 5112 5537
Size: 393 1274 787 162 66 250 941 425
Cuts at: 5537 5670 6743 7025 7233 7422 7476 8843 9440
Size: 133 1073 282 208 189 54 1367 597
Cuts at: 9440 10227 10495 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1367 1274 1073 941 787 787 597
425 393 282 268 250 208 189 162
133 66 54
EcoNI CCTnn'n_nnAGG
Cuts at: 5844 5844
Size: 10780
EcoO109I rG'GnC_Cy
Cuts at: 2956 3114 3721 6580 7770 2956
Size: 158 607 2859 1190 5966
Fragments arranged by size:
5966 2859 1190 607 158
EcoRI G'AATT_C
Cuts at: 4461 4979 5240 8089 8467 4461
Size: 518 261 2849 378 6774
Fragments arranged by size:
6774 2849 518 378 261
EcoRV GAT'ATC
Cuts at: 5986 5986
Size: 10780
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3421 4556 6536 3421
Size: 1135 1980 7665
Fragments arranged by size:
7665 1980 1135
FspI TGC'GCA
Cuts at: 9286 9286
Size: 10780
HaeII r_GCGC'y
Cuts at: 1678 3531 7980 9927 1678
Size: 1853 4449 1947 2531
Fragments arranged by size:
4449 2531 1947 1853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3653 3685 3811 3843 4868
Size: 32 135 32 2081 32 126 32 1025
Cuts at: 4868 4880 4900 4912 5330 5362 5645 5677 5970
Size: 12 20 12 418 32 283 32 293
Cuts at: 5970 6002 7040 7072 7541 7573 9421 9453 9495
Size: 32 1038 32 469 32 1848 32 42
Cuts at: 9495 9527 1373
Size: 32 2626
Fragments arranged by size:
2626 2081 1848 1038 1025 469 418 293
283 135 126 42 32 32 32 32
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3843 8124 8370 669
Size: 988 281 1905 4281 246 3079
Fragments arranged by size:
4281 3079 1905 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3478 748
Size: 2730 8050
HpaI GTT'AAC
Cuts at: 3843 8370 3843
Size: 4527 6253
KpnI G_GTAC'C
Cuts at: 8105 8105
Size: 10780
MfeI C'AATT_G
Cuts at: 2562 2569 3584 8379 2562
Size: 7 1015 4795 4963
Fragments arranged by size:
4963 4795 1015 7
MluI A'CGCG_T
Cuts at: 10656 10656
Size: 10780
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5802 9894 10078 1305
Size: 4497 4092 184 2007
Fragments arranged by size:
4497 4092 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2278 2899 3212 3596 6062
Size: 54 1425 343 446 621 313 384 2466
Cuts at: 6062 7263 7437 10
Size: 1201 174 3353
Fragments arranged by size:
3353 2466 1425 1201 621 446 384 343
313 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2328 6014 6174 7760 8738 9097
Size: 648 907 255 3686 160 1586 978 359
Cuts at: 9097 9256 518
Size: 159 2042
Fragments arranged by size:
3686 2042 1586 978 907 648 359 255
160 159
MspA1I CmG'CkG
Cuts at: 1606 4038 5004 5633 7414 8196 8873 10021 10266
Size: 2432 966 629 1781 782 677 1148 245
Cuts at: 10266 1606
Size: 2120
Fragments arranged by size:
2432 2120 1781 1148 966 782 677 629
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3973 513
Size: 552 939 1969 7320
Fragments arranged by size:
7320 1969 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10780
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10780
NotI GC'GGCC_GC
Cuts at: 8227 8227
Size: 10780
NspI r_CATG'y
Cuts at: 1132 1229 3035 4679 8138 9683 1132
Size: 97 1806 1644 3459 1545 2229
Fragments arranged by size:
3459 2229 1806 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 7352 7777 1348
Size: 6004 425 4351
Fragments arranged by size:
6004 4351 425
PciI A'CATG_T
Cuts at: 9679 9679
Size: 10780
PflMI CCAn_nnn'nTGG
Cuts at: 1108 7391 1108
Size: 6283 4497
PfoI T'CCnGG_A
Cuts at: 3832 7492 3832
Size: 3660 7120
PmeI GTTT'AAAC
Cuts at: 8084 8084
Size: 10780
PmlI CAC'GTG
Cuts at: 2071 2351 6385 2071
Size: 280 4034 6466
Fragments arranged by size:
6466 4034 280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7622 7654 8849 8881 10362 10394 7622
Size: 32 1195 32 1481 32 8008
Fragments arranged by size:
8008 1481 1195 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6580 7770 6580
Size: 1190 9590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10780
PsiI TTA'TAA
Cuts at: 8350 8350
Size: 10780
PspOMI G'GGCC_C
Cuts at: 2955 2955
Size: 10780
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4416 4448 6786 6818 4416
Size: 32 2338 32 8378
Fragments arranged by size:
8378 2338 32 32
PstI C_TGCA'G
Cuts at: 830 2207 5197 5404 6263 6925 7039 8132 830
Size: 1377 2990 207 859 662 114 1093 3478
Fragments arranged by size:
3478 2990 1377 1093 859 662 207 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10780
PvuII CAG'CTG
Cuts at: 4038 5633 7414 4038
Size: 1595 1781 7404
Fragments arranged by size:
7404 1781 1595
SacI G_AGCT'C
Cuts at: 2821 2821
Size: 10780
SalI G'TCGA_C
Cuts at: 1936 8122 1936
Size: 6186 4594
SapI GCTCTTCn'nnn_
Cuts at: 4401 8077 4401
Size: 3676 7104
SbfI CC_TGCA'GG
Cuts at: 8132 8132
Size: 10780
ScaI AGT'ACT
Cuts at: 4049 9028 10740 4049
Size: 4979 1712 4089
Fragments arranged by size:
4979 4089 1712
SexAI A'CCwGG_T
Cuts at: 6022 7766 6022
Size: 1744 9036
SfcI C'TryA_G
Cuts at: 826 1045 2203 4422 4466 4857 5193 5400 6259
Size: 219 1158 2219 44 391 336 207 859
Cuts at: 6259 6921 6971 7035 8128 9263 9944 10135 826
Size: 662 50 64 1093 1135 681 191 1471
Fragments arranged by size:
2219 1471 1158 1135 1093 859 681 662
391 336 219 207 191 64 50 44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10780
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10780
SmaI CCC'GGG
Cuts at: 1872 8107 1872
Size: 6235 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 4378 5537 5903 6224 6557
Size: 197 926 444 1991 1159 366 321 333
Cuts at: 6557 8884 9785 10047 820
Size: 2327 901 262 1553
Fragments arranged by size:
2327 1991 1553 1159 926 901 444 366
333 321 262 197
SnaBI TAC'GTA
Cuts at: 493 5961 8571 493
Size: 5468 2610 2702
Fragments arranged by size:
5468 2702 2610
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10780
SphI G_CATG'C
Cuts at: 1132 8138 1132
Size: 7006 3774
SspI AAT'ATT
Cuts at: 5 52 4668 8704 5
Size: 47 4616 4036 2081
Fragments arranged by size:
4616 4036 2081 47
StuI AGG'CCT
Cuts at: 1517 3539 1517
Size: 2022 8758
StyI C'CwwG_G
Cuts at: 513 1065 2004 3125 3643 3973 5055 5373 6057
Size: 552 939 1121 518 330 1082 318 684
Cuts at: 6057 7264 7818 8219 513
Size: 1207 554 401 3074
Fragments arranged by size:
3074 1207 1121 1082 939 684 554 552
518 401 330 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 4411 6005 7825 7850 8807 8824
Size: 1255 558 2273 1594 1820 25 957 17
Cuts at: 8824 8977 9162 9607 325
Size: 153 185 445 1498
Fragments arranged by size:
2273 1820 1594 1498 1255 957 558 445
185 153 25 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4047 5006 6036
Size: 274 80 33 51 1443 2070 959 1030
Cuts at: 6036 9026 10738 96
Size: 2990 1712 138
Fragments arranged by size:
2990 2070 1712 1443 1030 959 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6940 6972 7210 7242 10740 10772 6940
Size: 32 238 32 3498 32 6948
Fragments arranged by size:
6948 3498 238 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7768 1316
Size: 6452 4328
XbaI T'CTAG_A
Cuts at: 3781 5789 7687 8116 3781
Size: 2008 1898 429 6445
Fragments arranged by size:
6445 2008 1898 429
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6776 6776
Size: 10780
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10780
XmaI C'CCGG_G
Cuts at: 1870 8105 1870
Size: 6235 4545
XmnI GAAnn'nnTTC
Cuts at: 4242 5125 6345 6999 7080 7683 8909 4242
Size: 883 1220 654 81 603 1226 6113
Fragments arranged by size:
6113 1226 1220 883 654 603 81
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8588 276
Size: 53 83 186 936 7054 2468
Fragments arranged by size:
7054 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI RsrII SacII SanDI SfiI SfoI SrfI
SwaI