Restriction map for
pFN21AE3711
(Circular) (Six-base) MAPSORT of: pFN21AE3711.seq Check: 8277 from: 1 to: 5782
pFN21AE3711 5782 bp
With 182 enzymes: SgfI *
December 8, 2014 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 2963 3103 2963
Size: 140 5642
AclI AA'CG_TT
Cuts at: 1563 3081 3909 4282 1563
Size: 1518 828 373 3063
Fragments arranged by size:
3063 1518 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2483 2639 3845 5229 5497 1632
Size: 851 156 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 851 268 156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2592 4681 5658 1203
Size: 1389 2089 977 1327
Fragments arranged by size:
2089 1389 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 5750
AlwNI CAG_nnn'CTG
Cuts at: 2168 3525 5097 2168
Size: 1357 1572 2853
Fragments arranged by size:
2853 1572 1357
ApaI G_GGCC'C
Cuts at: 2955 2955
Size: 5782
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2743 3091 3317 3469 5578 1493
Size: 1250 348 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 348 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2441 2807 3107 1870
Size: 73 498 366 300 4545
Fragments arranged by size:
4545 498 366 300 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 2844 2963 3103 4558
Size: 325 217 627 576 481 119 140 1455
Cuts at: 4558 618
Size: 1842
Fragments arranged by size:
1842 1455 627 576 481 325 217 140
119
BanII G_rGCy'C
Cuts at: 1391 2511 2808 2955 2984 3065 3101 1391
Size: 1120 297 147 29 81 36 4072
Fragments arranged by size:
4072 1120 297 147 81 36 29
BbeI G_GCGC'C
Cuts at: 2848 2848
Size: 5782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2432 2466 3973 4007 1903
Size: 34 495 34 1507 34 3678
Fragments arranged by size:
3678 1507 495 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BfrBI ATG'CAT
Cuts at: 3073 3073
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2853 4393 136
Size: 107 122 71 631 605 1181 1540 1525
Fragments arranged by size:
1540 1525 1181 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 2111 2579 3199 2111
Size: 468 620 4694
Fragments arranged by size:
4694 620 468
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2762 2955 3843 4999 1790
Size: 472 500 193 888 1156 2573
Fragments arranged by size:
2573 1156 888 500 472 193
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3035 3513 4471
Size: 416 53 83 399 24 1618 478 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1618 958 478 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 2151 2396 2759 4442 1239
Size: 792 120 245 363 1683 2579
Fragments arranged by size:
2579 1683 792 363 245 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 2913 4445 882
Size: 617 771 643 1532 2219
Fragments arranged by size:
2219 1532 771 643 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 2649 5776 2649
Size: 3127 2655
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2845 3589 3971 4606
Size: 53 83 186 936 1312 744 382 635
Cuts at: 4606 275
Size: 1451
Fragments arranged by size:
1451 1312 936 744 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2295 2325 2732 2762 1178
Size: 30 165 30 892 30 407 30 4198
Fragments arranged by size:
4198 892 407 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2995 1186
Size: 1809 3973
BseYI C'CCAG_C
Cuts at: 2088 2793 2903 4985 5481 2088
Size: 705 110 2082 496 2389
Fragments arranged by size:
2389 2082 705 496 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2332 2979 1163
Size: 573 596 647 3966
Fragments arranged by size:
3966 647 596 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3843 3928 4999 3843
Size: 85 1071 4626
Fragments arranged by size:
4626 1071 85
BsmI GAATG_Cn'
Cuts at: 3073 3293 3386 3073
Size: 220 93 5469
Fragments arranged by size:
5469 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2511 2762 2808 2955 2984 3065
Size: 399 472 249 251 46 147 29 81
Cuts at: 3065 3101 3843 3928 4999 1391
Size: 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 251 249 147
85 81 46 36 29
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3137 844
Size: 2293 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2841 4426 4613 1700
Size: 110 1031 1585 187 2869
Fragments arranged by size:
2869 1585 1031 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 2801
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2238 3021 2238
Size: 783 4999
BtgI C'CryG_G
Cuts at: 513 1065 2004 2069 513
Size: 552 939 65 4226
Fragments arranged by size:
4226 939 552 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2773 487
Size: 683 56 1547 3496
Fragments arranged by size:
3496 1547 683 56
BtsI GCAGTG_nn'
Cuts at: 2762 2771 2991 3298 4092 4112 2762
Size: 9 220 307 794 20 4432
Fragments arranged by size:
4432 794 307 220 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747
Size: 54 1425 50 275 18 1399 889 1629
Cuts at: 5747 8
Size: 43
Fragments arranged by size:
1629 1425 1399 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2465 3712 1353
Size: 1112 1247 3423
Fragments arranged by size:
3423 1247 1112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 4369 4743 4889 387
Size: 1064 138 504 2276 374 146 1280
Fragments arranged by size:
2276 1280 1064 504 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2151 2396 2483 2639 2759 3845
Size: 393 399 120 245 87 156 120 1086
Cuts at: 3845 4442 5229 5497 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1086 787 597 399 393 268 245
156 120 120 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2130 2684 2916 2130
Size: 554 232 4996
Fragments arranged by size:
4996 554 232
EcoO109I rG'GnC_Cy
Cuts at: 2123 2331 2951 2952 2123
Size: 208 620 1 4953
Fragments arranged by size:
4953 620 208 1
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2848 4929 1678
Size: 1170 2081 2531
Fragments arranged by size:
2531 2081 1170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373
Size: 32 135 32 2851 32 42 32 2626
Fragments arranged by size:
2851 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2113 748
Size: 1365 4417
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KasI G'GCGC_C
Cuts at: 2844 2844
Size: 5782
KpnI G_GTAC'C
Cuts at: 2967 3107 2967
Size: 140 5642
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2224 4896 5080 1305
Size: 919 2672 184 2007
Fragments arranged by size:
2672 2007 919 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3740 4099 4258 518
Size: 648 2574 359 159 2042
Fragments arranged by size:
2574 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3198 3875 5023 5268 1606
Size: 1592 677 1148 245 2120
Fragments arranged by size:
2120 1592 1148 677 245
NarI GG'CG_CC
Cuts at: 2845 2845
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 2004 2069 513
Size: 552 939 65 4226
Fragments arranged by size:
4226 939 552 65
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NsiI A_TGCA'T
Cuts at: 3075 3075
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2596 3140 4685 1132
Size: 97 1367 544 1545 2229
Fragments arranged by size:
2229 1545 1367 544 97
PasI CC'CwG_GG
Cuts at: 1348 2482 2948 1348
Size: 1134 466 4182
Fragments arranged by size:
4182 1134 466
PciI A'CATG_T
Cuts at: 2592 4681 2592
Size: 2089 3693
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2054 2975 1108
Size: 946 921 3915
Fragments arranged by size:
3915 946 921
PfoI T'CCnGG_A
Cuts at: 2128 2128
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 3851 3883 5364 5396 2732
Size: 32 1087 32 1481 32 3118
Fragments arranged by size:
3118 1481 1087 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2929 1613
Size: 1316 4466
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PspOMI G'GGCC_C
Cuts at: 2951 2951
Size: 5782
PspXI vC'TCGA_Gb
Cuts at: 2441 2441
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2433 2577 2619 2757 2778 3134 830
Size: 1603 144 42 138 21 356 3478
Fragments arranged by size:
3478 1603 356 144 138 42 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2429 2573 2615 2753 2774 3130 4265
Size: 219 1384 144 42 138 21 356 1135
Cuts at: 4265 4946 5137 826
Size: 681 191 1471
Fragments arranged by size:
1471 1384 1135 681 356 219 191 144
138 42 21
SfoI GGC'GCC
Cuts at: 2846 2846
Size: 5782
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2441 2807 3886 4787 5049 820
Size: 197 926 498 366 1079 901 262 1553
Fragments arranged by size:
1553 1079 926 901 498 366 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2004 2069 2119 2263 3221 513
Size: 552 939 65 50 144 958 3074
Fragments arranged by size:
3074 958 939 552 144 65 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2614 3809 3826 3979 4164 4609 325
Size: 1255 1034 1195 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1195 1034 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3000 4028 5740
Size: 274 80 33 51 1443 1023 1028 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1712 1443 1028 1023 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2295 2327 5742 5774 2295
Size: 32 3415 32 2303
Fragments arranged by size:
3415 2303 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2126 2126
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 2441 2807 1943
Size: 498 366 4918
Fragments arranged by size:
4918 498 366
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 2537 2652 2747 3033 3911 2537
Size: 115 95 286 878 4408
Fragments arranged by size:
4408 878 286 115 95
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PpuMI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI