(Circular) (Six-base) MAPSORT of: pFN21AE3710.seq Check: 819 from: 1 to: 5569 pFN21AE3710 5569 bp With 182 enzymes: SgfI * March 28, 2012 12:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3379 278 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2061 2912 1937 Size: 124 851 4594 Fragments arranged by size: 4594 851 124 Acc65I G'GTAC_C Cuts at: 2890 2890 Size: 5569 AclI AA'CG_TT Cuts at: 1563 2313 3696 4069 1563 Size: 750 1383 373 3063 Fragments arranged by size: 3063 1383 750 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2133 3632 5016 5284 1632 Size: 501 1499 1384 268 1917 Fragments arranged by size: 1917 1499 1384 501 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 4468 5445 1203 Size: 3265 977 1327 Fragments arranged by size: 3265 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 4298 4298 Size: 5569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5569 AlwNI CAG_nnn'CTG Cuts at: 2449 3312 4884 2449 Size: 863 1572 3134 Fragments arranged by size: 3134 1572 863 ApaLI G'TGCA_C Cuts at: 3626 4782 3626 Size: 1156 4413 ApoI r'AATT_y Cuts at: 1493 2816 2878 3104 3256 5365 1493 Size: 1323 62 226 152 2109 1697 Fragments arranged by size: 2109 1697 1323 226 152 62 AseI AT'TA_AT Cuts at: 160 1032 2591 4123 160 Size: 872 1559 1532 1606 Fragments arranged by size: 1606 1559 1532 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2285 2894 1870 Size: 73 342 609 4545 Fragments arranged by size: 4545 609 342 73 BamHI G'GATC_C Cuts at: 2899 3266 2899 Size: 367 5202 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2291 2372 2665 2890 Size: 325 217 627 462 42 81 293 225 Cuts at: 2890 4345 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 462 325 293 225 217 81 42 BanII G_rGCy'C Cuts at: 1391 2200 2888 1391 Size: 809 688 4072 Fragments arranged by size: 4072 809 688 BbeI G_GCGC'C Cuts at: 2253 2376 2253 Size: 123 5446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4598 BbvCI CC'TCA_GC Cuts at: 2652 2652 Size: 5569 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3760 3794 1903 Size: 34 1823 34 3678 Fragments arranged by size: 3678 1823 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3461 4671 3461 Size: 1210 4359 BclI T'GATC_A Cuts at: 1548 1548 Size: 5569 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2370 2663 4180 Size: 107 122 71 631 605 698 293 1517 Cuts at: 4180 136 Size: 1525 Fragments arranged by size: 1525 1517 698 631 605 293 122 107 71 BglII A'GATC_T Cuts at: 5564 5564 Size: 5569 BlpI GC'TnA_GC Cuts at: 2048 2986 2048 Size: 938 4631 Bme1580I G_kGCm'C Cuts at: 1790 2294 3630 4786 1790 Size: 504 1336 1156 2573 Fragments arranged by size: 2573 1336 1156 504 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2475 3300 4258 Size: 416 53 83 399 24 1058 825 958 Cuts at: 4258 442 Size: 1753 Fragments arranged by size: 1753 1058 958 825 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2471 4229 1239 Size: 1169 63 1758 2579 Fragments arranged by size: 2579 1758 1169 63 Bpu10I CC'TnA_GC Cuts at: 2148 2154 2652 2148 Size: 6 498 5065 Fragments arranged by size: 5065 498 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3694 4559 4857 3694 Size: 865 298 4406 Fragments arranged by size: 4406 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4232 882 Size: 617 2733 2219 Fragments arranged by size: 2733 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3360 493 Size: 711 2156 2702 Fragments arranged by size: 2702 2156 711 BsaBI GATnn'nnATC Cuts at: 5563 5563 Size: 5569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 2373 3376 3758 Size: 53 83 186 936 717 123 1003 382 Cuts at: 3758 4393 275 Size: 635 1451 Fragments arranged by size: 1451 1003 936 717 635 382 186 123 83 53 BsaWI w'CCGG_w Cuts at: 2014 4002 4400 4674 4821 5232 2014 Size: 1988 398 274 147 411 2351 Fragments arranged by size: 2351 1988 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 1178 Size: 30 165 30 1035 30 4279 Fragments arranged by size: 4279 1035 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2587 2745 1186 Size: 955 446 158 4010 Fragments arranged by size: 4010 955 446 158 BseYI C'CCAG_C Cuts at: 4772 5268 4772 Size: 496 5073 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2558 1163 Size: 573 822 4174 Fragments arranged by size: 4174 822 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3780 4808 1540 Size: 275 186 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3630 3715 4786 3630 Size: 85 1071 4413 Fragments arranged by size: 4413 1071 85 BsmI GAATG_Cn' Cuts at: 2095 3080 3173 2095 Size: 985 93 4491 Fragments arranged by size: 4491 985 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2200 2294 2888 3630 3715 4786 1391 Size: 399 410 94 594 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 594 410 399 94 85 BspEI T'CCGG_A Cuts at: 2014 5232 2014 Size: 3218 2351 BspHI T'CATG_A Cuts at: 3458 5188 3458 Size: 1730 3839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2924 844 Size: 2080 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3456 1394 Size: 429 1633 3507 Fragments arranged by size: 3507 1633 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2432 4064 4238 65 Size: 1183 1184 1632 174 1396 Fragments arranged by size: 1632 1396 1184 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4213 4400 1700 Size: 110 2403 187 2869 Fragments arranged by size: 2869 2403 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5569 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5569 BssSI C'ACGA_G Cuts at: 3629 4641 3629 Size: 1012 4557 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5569 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 2641 1184 Size: 1457 4112 BstYI r'GATC_y Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564 Size: 927 367 385 17 1452 86 12 346 Cuts at: 5564 1972 Size: 1977 Fragments arranged by size: 1977 1452 927 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4078 Fragments arranged by size: 4078 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2319 487 Size: 683 56 1093 3737 Fragments arranged by size: 3737 1093 683 56 BtsI GCAGTG_nn' Cuts at: 2541 2628 3085 3879 3899 2541 Size: 87 457 794 20 4211 Fragments arranged by size: 4211 794 457 87 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2263 2806 3016 Size: 54 1425 50 275 18 433 543 210 Cuts at: 3016 3905 5534 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 543 433 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3016 1537 Size: 275 1204 4090 Fragments arranged by size: 4090 1204 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2127 2554 3499 1353 Size: 756 18 427 945 3423 Fragments arranged by size: 3423 945 756 427 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2220 2241 4156 4530 4676 387 Size: 1064 138 631 21 1915 374 146 1280 Fragments arranged by size: 1915 1280 1064 631 374 146 138 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2133 2408 2471 3632 4229 5016 5284 Size: 393 501 275 63 1161 597 787 268 Cuts at: 5284 1239 Size: 1524 Fragments arranged by size: 1524 1161 787 597 501 393 275 268 63 EcoNI CCTnn'n_nnAGG Cuts at: 2157 2157 Size: 5569 EcoRI G'AATT_C Cuts at: 2878 3256 2878 Size: 378 5191 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 2253 2376 4716 1678 Size: 575 123 2340 2531 Fragments arranged by size: 2531 2340 575 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2563 2595 4210 4242 4284 Size: 32 135 32 991 32 1615 32 42 Cuts at: 4284 4316 1373 Size: 32 2626 Fragments arranged by size: 2626 1615 991 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2062 2913 3159 669 Size: 988 281 124 851 246 3079 Fragments arranged by size: 3079 988 851 281 246 124 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KasI G'GCGC_C Cuts at: 2249 2372 2249 Size: 123 5446 KpnI G_GTAC'C Cuts at: 2894 2894 Size: 5569 MfeI C'AATT_G Cuts at: 3168 3168 Size: 5569 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2488 4683 4867 1305 Size: 1183 2195 184 2007 Fragments arranged by size: 2195 2007 1183 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2265 2808 10 Size: 54 1425 343 433 543 2771 Fragments arranged by size: 2771 1425 543 433 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2519 2621 3527 3886 4045 518 Size: 648 1353 102 906 359 159 2042 Fragments arranged by size: 2042 1353 906 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2298 2440 2651 2682 2804 2985 3662 4810 Size: 692 142 211 31 122 181 677 1148 Cuts at: 4810 5055 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 692 677 245 211 181 142 122 31 NarI GG'CG_CC Cuts at: 2250 2373 2250 Size: 123 5446 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4078 Fragments arranged by size: 4078 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2608 2927 4472 1132 Size: 97 1379 319 1545 2229 Fragments arranged by size: 2229 1545 1379 319 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5569 PciI A'CATG_T Cuts at: 4468 4468 Size: 5569 PflMI CCAn_nnn'nTGG Cuts at: 1108 2804 1108 Size: 1696 3873 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 5151 5183 3638 Size: 32 1481 32 4024 Fragments arranged by size: 4024 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PspXI vC'TCGA_Gb Cuts at: 2285 2285 Size: 5569 PstI C_TGCA'G Cuts at: 830 2059 2445 2921 830 Size: 1229 386 476 3478 Fragments arranged by size: 3478 1229 476 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 PvuII CAG'CTG Cuts at: 2440 2651 2440 Size: 211 5358 SalI G'TCGA_C Cuts at: 1936 2060 2911 1936 Size: 124 851 4594 Fragments arranged by size: 4594 851 124 SapI GCTCTTCn'nnn_ Cuts at: 2554 2554 Size: 5569 SbfI CC_TGCA'GG Cuts at: 2921 2921 Size: 5569 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SfcI C'TryA_G Cuts at: 826 1045 2055 2078 2441 2917 4052 4733 4924 Size: 219 1010 23 363 476 1135 681 191 Cuts at: 4924 826 Size: 1471 Fragments arranged by size: 1471 1135 1010 681 476 363 219 191 23 SfoI GGC'GCC Cuts at: 2251 2374 2251 Size: 123 5446 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5569 SmaI CCC'GGG Cuts at: 1872 2896 1872 Size: 1024 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2285 3673 4574 4836 820 Size: 197 926 342 1388 901 262 1553 Fragments arranged by size: 1553 1388 926 901 342 262 197 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2927 1132 Size: 1795 3774 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5569 StyI C'CwwG_G Cuts at: 513 1065 2004 2200 3008 513 Size: 552 939 196 808 3074 Fragments arranged by size: 3074 939 808 552 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3596 3613 3766 3951 4396 325 Size: 1255 2016 17 153 185 445 1498 Fragments arranged by size: 2016 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3815 5527 96 Size: 274 80 33 51 1443 1838 1712 138 Fragments arranged by size: 1838 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 2702 2734 5529 5561 2438 Size: 32 232 32 2795 32 2446 Fragments arranged by size: 2795 2446 232 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5569 XbaI T'CTAG_A Cuts at: 2905 2905 Size: 5569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2816 2816 Size: 5569 XhoI C'TCGA_G Cuts at: 1943 2285 1943 Size: 342 5227 XmaI C'CCGG_G Cuts at: 1870 2894 1870 Size: 1024 4545 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3377 276 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI