Restriction map for
pFN21AE3709
(Circular) (Six-base) MAPSORT of: pFN21AE3709.seq Check: 9214 from: 1 to: 5341
pFN21AE3709 5341 bp
With 182 enzymes: SgfI *
March 28, 2012 12:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 1937
Size: 747 4594
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2384 3404 4788 5056 1632
Size: 446 306 1020 1384 268 1917
Fragments arranged by size:
1917 1384 1020 446 306 268
AfeI AGC'GCT
Cuts at: 2389 2389
Size: 5341
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 2499 2539 4240 5217 1203
Size: 1296 40 1701 977 1327
Fragments arranged by size:
1701 1327 1296 977 40
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 2454 1166
Size: 1288 4053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 3084 4656 3084
Size: 1572 3769
ApaLI G'TGCA_C
Cuts at: 3398 4554 3398
Size: 1156 4185
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2666 1870
Size: 73 723 4545
Fragments arranged by size:
4545 723 73
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2662 4117 618
Size: 325 217 627 875 1455 1842
Fragments arranged by size:
1842 1455 875 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2660 1391
Size: 1269 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 2253 1548
Size: 705 4636
BfrBI ATG'CAT
Cuts at: 2031 2031
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 3952 136
Size: 107 122 71 631 605 412 1868 1525
Fragments arranged by size:
1868 1525 631 605 412 122 107 71
BglII A'GATC_T
Cuts at: 2308 2526 5336 2308
Size: 218 2810 2313
Fragments arranged by size:
2810 2313 218
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 3402 4558 1790
Size: 1612 1156 2573
Fragments arranged by size:
2573 1612 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2203 3072 4030
Size: 416 53 83 399 24 786 869 958
Cuts at: 4030 442
Size: 1753
Fragments arranged by size:
1753 958 869 786 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2657 4001 1239
Size: 1418 1344 2579
Fragments arranged by size:
2579 1418 1344
Bpu10I CC'TnA_GC
Cuts at: 2211 2211
Size: 5341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2402 3466 4331 4629 2402
Size: 1064 865 298 3114
Fragments arranged by size:
3114 1064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4004 882
Size: 617 2505 2219
Fragments arranged by size:
2505 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2502 3132 493
Size: 711 1298 630 2702
Fragments arranged by size:
2702 1298 711 630
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2150 3148 3530 4165
Size: 53 83 186 936 617 998 382 635
Cuts at: 4165 275
Size: 1451
Fragments arranged by size:
1451 998 936 635 617 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2155 3774 4172 4446 4593 5004 2155
Size: 1619 398 274 147 411 2492
Fragments arranged by size:
2492 1619 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 1178
Size: 30 165 30 939 30 4147
Fragments arranged by size:
4147 939 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2363 1186
Size: 1096 81 4164
Fragments arranged by size:
4164 1096 81
BseYI C'CCAG_C
Cuts at: 4544 5040 4544
Size: 496 4845
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2283 1163
Size: 573 547 4221
Fragments arranged by size:
4221 573 547
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2791 3552 4580 1540
Size: 275 186 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3402 3487 4558 3402
Size: 85 1071 4185
Fragments arranged by size:
4185 1071 85
BsmI GAATG_Cn'
Cuts at: 2230 2852 2945 2230
Size: 622 93 4626
Fragments arranged by size:
4626 622 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2141 2141
Size: 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2660 3402 3487 4558 1391
Size: 399 870 742 85 1071 2174
Fragments arranged by size:
2174 1071 870 742 399 85
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2696 844
Size: 1852 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 3228 1394
Size: 429 344 1061 3507
Fragments arranged by size:
3507 1061 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3985 4172 1700
Size: 110 2175 187 2869
Fragments arranged by size:
2869 2175 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5341
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstXI CCAn_nnnn'nTGG
Cuts at: 2456 2456
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2223 2308 2526 2671 3038 3423 3440 4892
Size: 251 85 218 145 367 385 17 1452
Cuts at: 4892 4978 4990 5336 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 251 218 145
86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2140 513
Size: 552 939 136 3714
Fragments arranged by size:
3714 939 552 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2857 3651 3671 2857
Size: 794 20 4527
Fragments arranged by size:
4527 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2788 3677 5306
Size: 54 1425 50 275 18 958 889 1629
Cuts at: 5306 8
Size: 43
Fragments arranged by size:
1629 1425 958 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 1537
Size: 275 976 4090
Fragments arranged by size:
4090 976 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3271 1353
Size: 1918 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2384 2657 3404 4001 4788 5056
Size: 393 446 306 273 747 597 787 268
Cuts at: 5056 1239
Size: 1524
Fragments arranged by size:
1524 787 747 597 446 393 306 273
268
EcoO109I rG'GnC_Cy
Cuts at: 2098 2131 2098
Size: 33 5308
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 2391 4488 1678
Size: 713 2097 2531
Fragments arranged by size:
2531 2097 713
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2241 2273 2423
Size: 32 135 32 548 32 89 32 150
Cuts at: 2423 2455 3982 4014 4056 4088 1373
Size: 32 1527 32 42 32 2626
Fragments arranged by size:
2626 1527 548 150 135 89 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2685 2931 669
Size: 988 281 747 246 3079
Fragments arranged by size:
3079 988 747 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3519
Fragments arranged by size:
3519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2454 3299 3658 3817 518
Size: 648 1288 845 359 159 2042
Fragments arranged by size:
2042 1288 845 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2757 3434 4582 4827 1606
Size: 1151 677 1148 245 2120
Fragments arranged by size:
2120 1151 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3850
Fragments arranged by size:
3850 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NsiI A_TGCA'T
Cuts at: 2033 2033
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2031 2543 2699 4244 1132
Size: 97 802 512 156 1545 2229
Fragments arranged by size:
2229 1545 802 512 156 97
PasI CC'CwG_GG
Cuts at: 1348 2625 1348
Size: 1277 4064
PciI A'CATG_T
Cuts at: 2539 4240 2539
Size: 1701 3640
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2631 1108
Size: 1523 3818
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2098
Size: 5341
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2693 830
Size: 1863 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SfcI C'TryA_G
Cuts at: 826 1045 2689 3824 4505 4696 826
Size: 219 1644 1135 681 191 1471
Fragments arranged by size:
1644 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2668 1872
Size: 796 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2417 3445 4346 4608 820
Size: 197 926 474 1028 901 262 1553
Fragments arranged by size:
1553 1028 926 901 474 262 197
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2004 2780 513
Size: 552 939 776 3074
Fragments arranged by size:
3074 939 776 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 3385 3538 3723 4168 325
Size: 1255 1788 17 153 185 445 1498
Fragments arranged by size:
1788 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3587 5299 96
Size: 274 80 33 51 1443 1610 1712 138
Fragments arranged by size:
1712 1610 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 5301 5333 2342
Size: 32 2927 32 2350
Fragments arranged by size:
2927 2350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2632 2632
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2666 1870
Size: 796 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI