Restriction map for
pFN21AE3689
(Circular) (Six-base) MAPSORT of: pFN21AE3689.seq Check: 1651 from: 1 to: 6760
pFN21AE3689 6760 bp
With 182 enzymes: SgfI *
March 28, 2012 14:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4570 278
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4103 1937
Size: 2166 4594
Acc65I G'GTAC_C
Cuts at: 4081 4081
Size: 6760
AclI AA'CG_TT
Cuts at: 1563 4887 5260 1563
Size: 3324 373 3063
Fragments arranged by size:
3324 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2168 2904 2915 3597 4823 6207 6475
Size: 401 135 736 11 682 1226 1384 268
Cuts at: 6475 1632
Size: 1917
Fragments arranged by size:
1917 1384 1226 736 682 401 268 135
11
AfeI AGC'GCT
Cuts at: 3479 3479
Size: 6760
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6563
AflIII A'CryG_T
Cuts at: 1203 5659 6636 1203
Size: 4456 977 1327
Fragments arranged by size:
4456 1327 977
AgeI A'CCGG_T
Cuts at: 5591 5591
Size: 6760
AhdI GACnn_n'nnGTC
Cuts at: 5489 5489
Size: 6760
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4038 1184
Size: 2854 3906
AlwNI CAG_nnn'CTG
Cuts at: 4503 6075 4503
Size: 1572 5188
ApaLI G'TGCA_C
Cuts at: 4817 5973 4817
Size: 1156 5604
ApoI r'AATT_y
Cuts at: 1493 2406 2889 2918 3883 4069 4295 4447 6556
Size: 913 483 29 965 186 226 152 2109
Cuts at: 6556 1493
Size: 1697
Fragments arranged by size:
2109 1697 965 913 483 226 186 152
29
AseI AT'TA_AT
Cuts at: 160 1032 3104 5314 160
Size: 872 2072 2210 1606
Fragments arranged by size:
2210 2072 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 2966 4085 1870
Size: 73 249 774 1119 4545
Fragments arranged by size:
4545 1119 774 249 73
BamHI G'GATC_C
Cuts at: 2119 4090 4457 2119
Size: 1971 367 4422
Fragments arranged by size:
4422 1971 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4081 5536 618
Size: 325 217 627 2294 1455 1842
Fragments arranged by size:
2294 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2253 4079 1391
Size: 862 1826 4072
Fragments arranged by size:
4072 1826 862
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 2346 3462 928
Size: 971 257 190 1116 4226
Fragments arranged by size:
4226 1116 971 257 190
BbvCI CC'TCA_GC
Cuts at: 3723 3723
Size: 6760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2268 2302 3960 3994 4951 4985 1903
Size: 34 331 34 1658 34 957 34 3678
Fragments arranged by size:
3678 1658 957 331 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4652 5862 4652
Size: 1210 5550
BclI T'GATC_A
Cuts at: 1548 2695 1548
Size: 1147 5613
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5371 136
Size: 107 122 71 631 605 3699 1525
Fragments arranged by size:
3699 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6755 6755
Size: 6760
BlpI GC'TnA_GC
Cuts at: 2132 3481 4177 2132
Size: 1349 696 4715
Fragments arranged by size:
4715 1349 696
Bme1580I G_kGCm'C
Cuts at: 1790 4821 5977 1790
Size: 3031 1156 2573
Fragments arranged by size:
3031 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6760
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2422 4491 5449
Size: 416 53 83 399 24 1005 2069 958
Cuts at: 5449 442
Size: 1753
Fragments arranged by size:
2069 1753 1005 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3681 5420 1239
Size: 2442 1739 2579
Fragments arranged by size:
2579 2442 1739
Bpu10I CC'TnA_GC
Cuts at: 3723 3723
Size: 6760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 3585 4885 5750 6048 2249
Size: 1336 1300 865 298 2961
Fragments arranged by size:
2961 1336 1300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3603 5423 882
Size: 617 2104 1820 2219
Fragments arranged by size:
2219 2104 1820 617
BsaAI yAC'GTr
Cuts at: 493 1204 2453 4551 493
Size: 711 1249 2098 2702
Fragments arranged by size:
2702 2098 1249 711
BsaBI GATnn'nnATC
Cuts at: 6754 6754
Size: 6760
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4567 4949 5584 275
Size: 53 83 186 936 3034 382 635 1451
Fragments arranged by size:
3034 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2122 5193 5591 5865 6012 6423 2122
Size: 3071 398 274 147 411 2459
Fragments arranged by size:
3071 2459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6535
Fragments arranged by size:
6535 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 1186
Size: 835 5925
BseYI C'CCAG_C
Cuts at: 2109 2505 2743 3253 3962 5963 6459 2109
Size: 396 238 510 709 2001 496 2410
Fragments arranged by size:
2410 2001 709 510 496 396 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2742 3836 1163
Size: 573 1006 1094 4087
Fragments arranged by size:
4087 1094 1006 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4210 4971 5999 1540
Size: 275 186 2209 761 1028 2301
Fragments arranged by size:
2301 2209 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 2607 4821 4906 5977 2253
Size: 354 2214 85 1071 3036
Fragments arranged by size:
3036 2214 1071 354 85
BsmI GAATG_Cn'
Cuts at: 4271 4364 4271
Size: 93 6667
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2607 4079 4821 4906 5977 1391
Size: 399 463 354 1472 742 85 1071 2174
Fragments arranged by size:
2174 1472 1071 742 463 399 354 85
BspEI T'CCGG_A
Cuts at: 6423 6423
Size: 6760
BspHI T'CATG_A
Cuts at: 4649 6379 4649
Size: 1730 5030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4115 844
Size: 3271 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4647 1394
Size: 429 2824 3507
Fragments arranged by size:
3507 2824 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5255 5429 65
Size: 1183 4007 174 1396
Fragments arranged by size:
4007 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3990 5404 5591 1700
Size: 110 2180 1414 187 2869
Fragments arranged by size:
2869 2180 1414 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6760
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6760
BssSI C'ACGA_G
Cuts at: 4820 5832 4820
Size: 1012 5748
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6760
BstBI TT'CG_AA
Cuts at: 5564 5564
Size: 6760
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6760
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2113
Size: 6760
BstYI r'GATC_y
Cuts at: 1972 2119 4090 4457 4842 4859 6311 6397 6409
Size: 147 1971 367 385 17 1452 86 12
Cuts at: 6409 6755 1972
Size: 346 1977
Fragments arranged by size:
1977 1971 1452 385 367 346 147 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2937 513
Size: 552 1872 4336
Fragments arranged by size:
4336 1872 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6021
Fragments arranged by size:
6021 683 56
BtsI GCAGTG_nn'
Cuts at: 4276 5070 5090 4276
Size: 794 20 5946
Fragments arranged by size:
5946 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2290 2325 529
Size: 35 1726 35 4964
Fragments arranged by size:
4964 1726 35 35
DraI TTT'AAA
Cuts at: 2614 3556 4064 2614
Size: 942 508 5310
Fragments arranged by size:
5310 942 508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5767 809
Size: 507 4451 1802
Fragments arranged by size:
4451 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 4207 5096
Size: 54 1425 50 275 18 228 2149 889
Cuts at: 5096 6725 8
Size: 1629 43
Fragments arranged by size:
2149 1629 1425 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4207 1537
Size: 275 2395 4090
Fragments arranged by size:
4090 2395 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2494 2776 3250 3308 3407 3573 4690 1353
Size: 1141 282 474 58 99 166 1117 3423
Fragments arranged by size:
3423 1141 1117 474 282 166 99 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5347 5721 5867 387
Size: 1064 138 3758 374 146 1280
Fragments arranged by size:
3758 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 2251
Size: 6760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2168 2904 2915 3597 3681 4823
Size: 393 401 135 736 11 682 84 1142
Cuts at: 4823 5420 6207 6475 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1142 787 736 682 597 401 393
268 135 84 11
EcoRI G'AATT_C
Cuts at: 4069 4447 4069
Size: 378 6382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2585 2585
Size: 6760
FspI TGC'GCA
Cuts at: 5266 5266
Size: 6760
HaeII r_GCGC'y
Cuts at: 1678 2132 3481 5907 1678
Size: 454 1349 2426 2531
Fragments arranged by size:
2531 2426 1349 454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2493 2505 2525 2537 2611
Size: 32 135 32 921 12 20 12 74
Cuts at: 2611 2643 3572 3604 4040 4072 5401 5433 5475
Size: 32 929 32 436 32 1329 32 42
Cuts at: 5475 5507 1373
Size: 32 2626
Fragments arranged by size:
2626 1329 929 921 436 135 74 42
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3831 4104 4350 669
Size: 988 281 1893 273 246 3079
Fragments arranged by size:
3079 1893 988 281 273 246
HindIII A'AGCT_T
Cuts at: 748 2015 748
Size: 1267 5493
HpaI GTT'AAC
Cuts at: 3831 4350 3831
Size: 519 6241
KpnI G_GTAC'C
Cuts at: 4085 4085
Size: 6760
MfeI C'AATT_G
Cuts at: 4359 4359
Size: 6760
MluI A'CGCG_T
Cuts at: 6636 6636
Size: 6760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3570 5874 6058 1305
Size: 2265 2304 184 2007
Fragments arranged by size:
2304 2265 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 10
Size: 54 1425 343 228 4710
Fragments arranged by size:
4710 1425 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2325 3717 3950 4718 5077 5236 518
Size: 648 1159 1392 233 768 359 159 2042
Fragments arranged by size:
2042 1392 1159 768 648 359 233 159
MspA1I CmG'CkG
Cuts at: 1606 2113 4176 4853 6001 6246 1606
Size: 507 2063 677 1148 245 2120
Fragments arranged by size:
2120 2063 1148 677 507 245
NcoI C'CATG_G
Cuts at: 513 1065 2937 513
Size: 552 1872 4336
Fragments arranged by size:
4336 1872 552
NdeI CA'TA_TG
Cuts at: 387 3787 4021 387
Size: 3400 234 3126
Fragments arranged by size:
3400 3126 234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6760
NotI GC'GGCC_GC
Cuts at: 4207 4207
Size: 6760
NspI r_CATG'y
Cuts at: 1132 1229 4118 5663 1132
Size: 97 2889 1545 2229
Fragments arranged by size:
2889 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6760
PciI A'CATG_T
Cuts at: 5659 5659
Size: 6760
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2082 2116 1108
Size: 974 34 5752
Fragments arranged by size:
5752 974 34
PfoI T'CCnGG_A
Cuts at: 2208 3697 2208
Size: 1489 5271
PmeI GTTT'AAAC
Cuts at: 4064 4064
Size: 6760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4829 4861 6342 6374 4829
Size: 32 1481 32 5215
Fragments arranged by size:
5215 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2388 1613
Size: 775 5985
PsiI TTA'TAA
Cuts at: 2658 4005 4330 2658
Size: 1347 325 5088
Fragments arranged by size:
5088 1347 325
PstI C_TGCA'G
Cuts at: 830 4112 830
Size: 3282 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6760
PvuII CAG'CTG
Cuts at: 2113 2113
Size: 6760
SacI G_AGCT'C
Cuts at: 2253 2253
Size: 6760
SalI G'TCGA_C
Cuts at: 1936 4102 1936
Size: 2166 4594
SbfI CC_TGCA'GG
Cuts at: 4112 4112
Size: 6760
ScaI AGT'ACT
Cuts at: 2548 5008 6720 2548
Size: 2460 1712 2588
Fragments arranged by size:
2588 2460 1712
SfcI C'TryA_G
Cuts at: 826 1045 3435 4108 5243 5924 6115 826
Size: 219 2390 673 1135 681 191 1471
Fragments arranged by size:
2390 1471 1135 681 673 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6760
SmaI CCC'GGG
Cuts at: 1872 4087 1872
Size: 2215 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 3600 4864 5765 6027 820
Size: 197 926 321 1336 1264 901 262 1553
Fragments arranged by size:
1553 1336 1264 926 901 321 262 197
SnaBI TAC'GTA
Cuts at: 493 2453 4551 493
Size: 1960 2098 2702
Fragments arranged by size:
2702 2098 1960
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6760
SphI G_CATG'C
Cuts at: 1132 4118 1132
Size: 2986 3774
SspI AAT'ATT
Cuts at: 5 52 3749 4684 5
Size: 47 3697 935 2081
Fragments arranged by size:
3697 2081 935 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6760
StyI C'CwwG_G
Cuts at: 513 1065 2394 2937 3277 4199 513
Size: 552 1329 543 340 922 3074
Fragments arranged by size:
3074 1329 922 552 543 340
SwaI ATTT'AAAT
Cuts at: 3556 3556
Size: 6760
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 3267 4787 4804 4957 5142 5587
Size: 1255 543 1144 1520 17 153 185 445
Cuts at: 5587 325
Size: 1498
Fragments arranged by size:
1520 1498 1255 1144 543 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2546 3269 3857
Size: 274 80 33 51 1443 569 723 588
Cuts at: 3857 5006 6718 96
Size: 1149 1712 138
Fragments arranged by size:
1712 1443 1149 723 588 569 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2677 2709 6720 6752 2677
Size: 32 4011 32 2685
Fragments arranged by size:
4011 2685 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6760
XbaI T'CTAG_A
Cuts at: 4096 4096
Size: 6760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2113
Size: 6760
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6760
XmaI C'CCGG_G
Cuts at: 1870 4085 1870
Size: 2215 4545
XmnI GAAnn'nnTTC
Cuts at: 4889 4889
Size: 6760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4568 276
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI