Restriction map for
pFN21AE3687
(Circular) (Six-base) MAPSORT of: pFN21AE3687.seq Check: 3038 from: 1 to: 6160
pFN21AE3687 6160 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2198 3970 278
Size: 53 83 186 936 662 1772 2468
Fragments arranged by size:
2468 1772 936 662 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3154 3503 1937
Size: 1217 349 4594
Fragments arranged by size:
4594 1217 349
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2430 2699 3306 4223 5607 5875 1632
Size: 798 269 607 917 1384 268 1917
Fragments arranged by size:
1917 1384 917 798 607 269 268
AflII C'TTAA_G
Cuts at: 820 1017 3088 820
Size: 197 2071 3892
Fragments arranged by size:
3892 2071 197
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 2940 1166
Size: 1774 4386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AlwNI CAG_nnn'CTG
Cuts at: 2130 3903 5475 2130
Size: 1773 1572 2815
Fragments arranged by size:
2815 1773 1572
ApaLI G'TGCA_C
Cuts at: 4217 5373 4217
Size: 1156 5004
ApoI r'AATT_y
Cuts at: 1493 2103 2213 2280 2535 3201 3271 3345 3469
Size: 610 110 67 255 666 70 74 124
Cuts at: 3469 3695 3847 5956 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 666 610 255 226 152 124
110 74 70 67
AseI AT'TA_AT
Cuts at: 160 1032 3035 3428 4714 160
Size: 872 2003 393 1286 1606
Fragments arranged by size:
2003 1606 1286 872 393
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 3485 1870
Size: 73 1542 4545
Fragments arranged by size:
4545 1542 73
BamHI G'GATC_C
Cuts at: 2776 3490 3857 2776
Size: 714 367 5079
Fragments arranged by size:
5079 714 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3481 4936 618
Size: 325 217 627 1694 1455 1842
Fragments arranged by size:
1842 1694 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3479 1391
Size: 2088 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 3000 928
Size: 971 143 958 4088
Fragments arranged by size:
4088 971 958 143
BbvCI CC'TCA_GC
Cuts at: 2828 2828
Size: 6160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4351 4385 1903
Size: 34 2414 34 3678
Fragments arranged by size:
3678 2414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2025 4052 5262 2025
Size: 2027 1210 2923
Fragments arranged by size:
2923 2027 1210
BclI T'GATC_A
Cuts at: 1548 2113 2860 1548
Size: 565 747 4848
Fragments arranged by size:
4848 747 565
BfrBI ATG'CAT
Cuts at: 3026 3026
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4771 136
Size: 107 122 71 631 605 3099 1525
Fragments arranged by size:
3099 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3337 6155 3337
Size: 2818 3342
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 4221 5377 1790
Size: 2431 1156 2573
Fragments arranged by size:
2573 2431 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2259 3891 4849
Size: 416 53 83 399 24 842 1632 958
Cuts at: 4849 442
Size: 1753
Fragments arranged by size:
1753 1632 958 842 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4820 1239
Size: 3581 2579
Bpu10I CC'TnA_GC
Cuts at: 2828 2828
Size: 6160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2887 4285 5150 5448 2887
Size: 1398 865 298 3599
Fragments arranged by size:
3599 1398 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4823 882
Size: 617 3324 2219
Fragments arranged by size:
3324 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2195 3967 4349 4984
Size: 53 83 186 936 662 1772 382 635
Cuts at: 4984 275
Size: 1451
Fragments arranged by size:
1772 1451 936 662 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5935
Fragments arranged by size:
5935 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 2890 1186
Size: 1210 494 4456
Fragments arranged by size:
4456 1210 494
BseYI C'CCAG_C
Cuts at: 2571 5363 5859 2571
Size: 2792 496 2872
Fragments arranged by size:
2872 2792 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5587
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3610 4371 5399 1540
Size: 275 186 1609 761 1028 2301
Fragments arranged by size:
2301 1609 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2937 4221 4306 5377 2937
Size: 1284 85 1071 3720
Fragments arranged by size:
3720 1284 1071 85
BsmI GAATG_Cn'
Cuts at: 3364 3671 3764 3364
Size: 307 93 5760
Fragments arranged by size:
5760 307 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2186 2186
Size: 6160
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2937 3479 4221 4306 5377 1391
Size: 399 1147 542 742 85 1071 2174
Fragments arranged by size:
2174 1147 1071 742 542 399 85
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3515 844
Size: 2671 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2838 4655 4829 65
Size: 1183 1590 1817 174 1396
Fragments arranged by size:
1817 1590 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4804 4991 1700
Size: 110 2994 187 2869
Fragments arranged by size:
2994 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2890 96
Size: 2794 3366
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6160
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 2776 3337 3490 3857 4242 4259 5711 5797
Size: 804 561 153 367 385 17 1452 86
Cuts at: 5797 5809 6155 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 804 561 385 367 346 153
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 2403 2611 2914 3676 4470 4490 2292
Size: 111 208 303 762 794 20 3962
Fragments arranged by size:
3962 794 762 303 208 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 2154 3270 3464 2154
Size: 1116 194 4850
Fragments arranged by size:
4850 1116 194
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3607 4496 6125
Size: 54 1425 50 275 18 1777 889 1629
Cuts at: 6125 8
Size: 43
Fragments arranged by size:
1777 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 2766 4090 1353
Size: 981 432 1324 3423
Fragments arranged by size:
3423 1324 981 432
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4747 5121 5267 387
Size: 1064 138 3158 374 146 1280
Fragments arranged by size:
3158 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2430 2699 3306 4223 4820 5607 5875
Size: 393 798 269 607 917 597 787 268
Cuts at: 5875 1239
Size: 1524
Fragments arranged by size:
1524 917 798 787 607 597 393 269
268
EcoRI G'AATT_C
Cuts at: 2280 3469 3847 2280
Size: 1189 378 4593
Fragments arranged by size:
4593 1189 378
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 5307 1678
Size: 3629 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4801 4833 4875 4907 1373
Size: 32 135 32 3229 32 42 32 2626
Fragments arranged by size:
3229 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2365 3172 3504 3750 669
Size: 988 281 427 807 332 246 3079
Fragments arranged by size:
3079 988 807 427 332 281 246
HindIII A'AGCT_T
Cuts at: 748 2553 748
Size: 1805 4355
HpaI GTT'AAC
Cuts at: 3172 3750 3172
Size: 578 5582
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 2421 3759 2421
Size: 1338 4822
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5274 5458 1305
Size: 3969 184 2007
Fragments arranged by size:
3969 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4338
Fragments arranged by size:
4338 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2856 2940 3006 3165 3216 4118 4477
Size: 648 1690 84 66 159 51 902 359
Cuts at: 4477 4636 518
Size: 159 2042
Fragments arranged by size:
2042 1690 902 648 359 159 159 84
66 51
MspA1I CmG'CkG
Cuts at: 1606 2940 3371 3576 4253 5401 5646 1606
Size: 1334 431 205 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 677 431 245 205
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5608
NdeI CA'TA_TG
Cuts at: 387 2670 387
Size: 2283 3877
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NsiI A_TGCA'T
Cuts at: 3028 3028
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2441 2759 3026 3030 3182 3518 5063
Size: 97 1212 318 267 4 152 336 1545
Cuts at: 5063 1132
Size: 2229
Fragments arranged by size:
2229 1545 1212 336 318 267 152 97
4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6160
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PfoI T'CCnGG_A
Cuts at: 2928 2928
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 2805 3730 2805
Size: 925 5235
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2904 2936 2904
Size: 32 6128
PstI C_TGCA'G
Cuts at: 830 3512 830
Size: 2682 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 2940 3371 2940
Size: 431 5729
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 2954 4408 6120 2954
Size: 1454 1712 2994
Fragments arranged by size:
2994 1712 1454
SexAI A'CCwGG_T
Cuts at: 2256 2256
Size: 6160
SfcI C'TryA_G
Cuts at: 826 1045 2304 2391 2427 3508 4643 5324 5515
Size: 219 1259 87 36 1081 1135 681 191
Cuts at: 5515 826
Size: 1471
Fragments arranged by size:
1471 1259 1135 1081 681 219 191 87
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 3487 1872
Size: 1615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2902 3088 4264 5165 5427 820
Size: 197 926 959 186 1176 901 262 1553
Fragments arranged by size:
1553 1176 959 926 901 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 2441 3026 3518 1132
Size: 1309 585 492 3774
Fragments arranged by size:
3774 1309 585 492
SspI AAT'ATT
Cuts at: 5 52 2159 2648 3257 3308 3317 4084 5
Size: 47 2107 489 609 51 9 767 2081
Fragments arranged by size:
2107 2081 767 609 489 51 47 9
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 3599 513
Size: 552 2534 3074
Fragments arranged by size:
3074 2534 552
SwaI ATTT'AAAT
Cuts at: 3270 3270
Size: 6160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2864 4187 4204 4357 4542 4987 325
Size: 1255 1284 1323 17 153 185 445 1498
Fragments arranged by size:
1498 1323 1284 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2272 2890 2952
Size: 274 80 33 51 1443 295 618 62
Cuts at: 2952 4406 6118 96
Size: 1454 1712 138
Fragments arranged by size:
1712 1454 1443 618 295 274 138 80
62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6120 6152 6120
Size: 32 6128
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 2284 3352 3496 2284
Size: 1068 144 4948
Fragments arranged by size:
4948 1068 144
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 3485 1870
Size: 1615 4545
XmnI GAAnn'nnTTC
Cuts at: 2415 4289 2415
Size: 1874 4286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2196 3968 276
Size: 53 83 186 936 662 1772 2468
Fragments arranged by size:
2468 1772 936 662 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI XcmI