Restriction map for
pFN21AE3682
(Circular) (Six-base) MAPSORT of: pFN21AE3682.seq Check: 4166 from: 1 to: 5956
pFN21AE3682 5956 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2173 2655 3074 2173
Size: 482 419 5055
Fragments arranged by size:
5055 482 419
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2325 4019 5403 5671 1632
Size: 693 1694 1384 268 1917
Fragments arranged by size:
1917 1694 1384 693 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 2574 4855 5832 1203
Size: 1371 2281 977 1327
Fragments arranged by size:
2281 1371 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 3025 3699 5271 3025
Size: 674 1572 3710
Fragments arranged by size:
3710 1572 674
ApaI G_GGCC'C
Cuts at: 2722 2722
Size: 5956
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2242 2635 2743 2768 3281 1870
Size: 73 271 28 393 108 25 513 4545
Fragments arranged by size:
4545 513 393 271 108 73 28 25
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2264 2278 2845 2884 3230
Size: 325 217 627 477 14 567 39 346
Cuts at: 3230 3277 4732 618
Size: 47 1455 1842
Fragments arranged by size:
1842 1455 627 567 477 346 325 217
47 39 14
BanII G_rGCy'C
Cuts at: 1391 2644 2722 3275 1391
Size: 1253 78 553 4072
Fragments arranged by size:
4072 1253 553 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 2118 2130 928
Size: 971 180 39 12 4754
Fragments arranged by size:
4754 971 180 39 12
BbvCI CC'TCA_GC
Cuts at: 2918 2918
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2789 2823 4147 4181 1903
Size: 34 852 34 1324 34 3678
Fragments arranged by size:
3678 1324 852 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BfrBI ATG'CAT
Cuts at: 2969 2969
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2532 3247 4567
Size: 107 122 71 631 605 860 715 1320
Cuts at: 4567 136
Size: 1525
Fragments arranged by size:
1525 1320 860 715 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 2022 2396 3373 2022
Size: 374 977 4605
Fragments arranged by size:
4605 977 374
Bme1580I G_kGCm'C
Cuts at: 1790 2281 2722 2848 3233 4017 5173 1790
Size: 491 441 126 385 784 1156 2573
Fragments arranged by size:
2573 1156 784 491 441 385 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3687 4645 442
Size: 416 53 83 399 24 2270 958 1753
Fragments arranged by size:
2270 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2569 3055 4616 1239
Size: 914 416 486 1561 2579
Fragments arranged by size:
2579 1561 914 486 416
Bpu10I CC'TnA_GC
Cuts at: 2918 2918
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2776 2947 3763 4145
Size: 53 83 186 936 1243 171 816 382
Cuts at: 4145 4780 275
Size: 635 1451
Fragments arranged by size:
1451 1243 936 816 635 382 186 171
83 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 1178
Size: 30 165 30 1146 30 4555
Fragments arranged by size:
4555 1146 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2144 2147 2944 3193 1186
Size: 928 30 3 797 249 3949
Fragments arranged by size:
3949 928 797 249 30 3
BseYI C'CCAG_C
Cuts at: 3222 5159 5655 3222
Size: 1937 496 3523
Fragments arranged by size:
3523 1937 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2658 2672 2777 1163
Size: 573 922 14 105 4342
Fragments arranged by size:
4342 922 573 105 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3047 3406 4167 5195 1540
Size: 275 186 1046 359 761 1028 2301
Fragments arranged by size:
2301 1046 1028 761 359 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 4017 4102 5173 2223
Size: 1794 85 1071 3006
Fragments arranged by size:
3006 1794 1071 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
BsmBI CGTCTCn'nnnn_
Cuts at: 2767 2767
Size: 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2281 2644 2722 2848 3233 3275
Size: 399 433 58 363 78 126 385 42
Cuts at: 3275 4017 4102 5173 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 385 363 126
85 78 58 42
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2019 2173 2655 2677 3074 3311 844
Size: 1175 154 482 22 397 237 3489
Fragments arranged by size:
3489 1175 482 397 237 154 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3013 3843 1394
Size: 429 1190 830 3507
Fragments arranged by size:
3507 1190 830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2320 4600 4787 1700
Size: 110 510 2280 187 2869
Fragments arranged by size:
2869 2280 510 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2508 3286 3653 4038 4055 5507 5593 5605
Size: 536 778 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 778 536 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2358 513
Size: 552 1293 4111
Fragments arranged by size:
4111 1293 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2329 487
Size: 683 56 1103 4114
Fragments arranged by size:
4114 1103 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 2085 1554
Size: 531 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2422 3403
Size: 54 1425 50 275 18 531 61 981
Cuts at: 3403 4292 5921 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 981 889 531 275 61 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2811 3886 1353
Size: 1458 1075 3423
Fragments arranged by size:
3423 1458 1075
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2462 2978 3117 4543 4917 5063
Size: 1064 138 873 516 139 1426 374 146
Cuts at: 5063 387
Size: 1280
Fragments arranged by size:
1426 1280 1064 873 516 374 146 139
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2325 2569 3055 4019 4616 5403
Size: 393 521 172 244 486 964 597 787
Cuts at: 5403 5671 1239
Size: 268 1524
Fragments arranged by size:
1524 964 787 597 521 486 393 268
244 172
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2096
Size: 5956
EcoO109I rG'GnC_Cy
Cuts at: 2705 2718 2719 2748 2705
Size: 13 1 29 5913
Fragments arranged by size:
5913 29 13 1
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 3029 4462 3029
Size: 1433 4523
HaeII r_GCGC'y
Cuts at: 1678 2029 2389 2623 3253 5103 1678
Size: 351 360 234 630 1850 2531
Fragments arranged by size:
2531 1850 630 360 351 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2208 2240 2549 2581 4597
Size: 32 135 32 636 32 309 32 2016
Cuts at: 4597 4629 4671 4703 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2016 636 309 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3603
Fragments arranged by size:
3603 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 3914 4273 4432 518
Size: 648 1191 1557 359 159 2042
Fragments arranged by size:
2042 1557 1191 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2400 3106 3139 3372 4049 5197 5442 1606
Size: 794 706 33 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 706 677 245 233 33
NaeI GCC'GGC
Cuts at: 2322 2322
Size: 5956
NcoI C'CATG_G
Cuts at: 513 1065 2358 513
Size: 552 1293 4111
Fragments arranged by size:
4111 1293 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2320 2320
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NsiI A_TGCA'T
Cuts at: 2971 2971
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 2578 3314 4859 1132
Size: 97 1349 736 1545 2229
Fragments arranged by size:
2229 1545 1349 736 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 2574 4855 2574
Size: 2281 3675
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PfoI T'CCnGG_A
Cuts at: 2055 2055
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2718 2718
Size: 5956
PstI C_TGCA'G
Cuts at: 830 2184 2903 3308 830
Size: 1354 719 405 3478
Fragments arranged by size:
3478 1354 719 405
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 2180 2899 3304 4439 5120 5311 826
Size: 219 1135 719 405 1135 681 191 1471
Fragments arranged by size:
1471 1135 1135 719 681 405 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 2637 2745 2770 3283 1872
Size: 765 108 25 513 4545
Fragments arranged by size:
4545 765 513 108 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2285 2358 2923 3209 3395 513
Size: 552 1220 73 565 286 186 3074
Fragments arranged by size:
3074 1220 565 552 286 186 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2544 2715 3983 4000 4153 4338 4783
Size: 1255 964 171 1268 17 153 185 445
Cuts at: 4783 325
Size: 1498
Fragments arranged by size:
1498 1268 1255 964 445 185 171 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 2635 2743 2768 3281 1870
Size: 765 108 25 513 4545
Fragments arranged by size:
4545 765 513 108 25
XmnI GAAnn'nnTTC
Cuts at: 2612 4085 2612
Size: 1473 4483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI