Restriction map for
pFN21AE3678
(Circular) (Six-base) MAPSORT of: pFN21AE3678.seq Check: 4661 from: 1 to: 5656
pFN21AE3678 5656 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2358 2977 2358
Size: 619 5037
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2445 2592 3719 5103 5371 1632
Size: 813 147 1127 1384 268 1917
Fragments arranged by size:
1917 1384 1127 813 268 147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 2221 4487 2221
Size: 2266 3390
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 2523 1166
Size: 1357 4299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2716 3399 4971 2716
Size: 683 1572 3401
Fragments arranged by size:
3401 1572 683
ApaI G_GGCC'C
Cuts at: 2214 2214
Size: 5656
ApaLI G'TGCA_C
Cuts at: 2440 3713 4869 2440
Size: 1273 1156 3227
Fragments arranged by size:
3227 1273 1156
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2297 2482 2981 1870
Size: 73 354 185 499 4545
Fragments arranged by size:
4545 499 354 185 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 2318 2358 2806 2977
Size: 325 217 627 431 100 40 448 171
Cuts at: 2977 4432 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 448 431 325 217 171
100 40
BanII G_rGCy'C
Cuts at: 1391 2214 2489 2496 2975 1391
Size: 823 275 7 479 4072
Fragments arranged by size:
4072 823 479 275 7
BbeI G_GCGC'C
Cuts at: 2322 2322
Size: 5656
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2484 928
Size: 971 585 4100
Fragments arranged by size:
4100 971 585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 2209 4267
Size: 107 122 71 631 605 373 164 2058
Cuts at: 4267 136
Size: 1525
Fragments arranged by size:
2058 1525 631 605 373 164 122 107
71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2444 2816 2840 3717 4873 1790
Size: 424 230 372 24 877 1156 2573
Fragments arranged by size:
2573 1156 877 424 372 230 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2726 2744 4316 1239
Size: 1487 18 1572 2579
Fragments arranged by size:
2579 1572 1487 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2236 3447 493
Size: 711 1032 1211 2702
Fragments arranged by size:
2702 1211 1032 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2319 3463 3845 4480
Size: 53 83 186 936 786 1144 382 635
Cuts at: 4480 275
Size: 1451
Fragments arranged by size:
1451 1144 936 786 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2221 4089 4487 4761 4908 5319 2221
Size: 1868 398 274 147 411 2558
Fragments arranged by size:
2558 1868 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2162 2660 2741 1186
Size: 973 3 498 81 4101
Fragments arranged by size:
4101 973 498 81 3
BseYI C'CCAG_C
Cuts at: 2314 2833 2892 4859 5355 2314
Size: 519 59 1967 496 2615
Fragments arranged by size:
2615 1967 519 496 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 3106 3867 4895 1540
Size: 275 186 36 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2444 2489 2952 3717 3802 4873 2444
Size: 45 463 765 85 1071 3227
Fragments arranged by size:
3227 1071 765 463 85 45
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2444 2489 2496 2816 2840 2952
Size: 399 424 230 45 7 320 24 112
Cuts at: 2952 2975 3717 3802 4873 1391
Size: 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 320 230 112
85 45 24 23 7
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 2496 3545 5275 2496
Size: 1049 1730 2877
Fragments arranged by size:
2877 1730 1049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2254 2731 3543 1394
Size: 429 431 477 812 3507
Fragments arranged by size:
3507 812 477 431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2152 2221 2803 4300 4487 1700
Size: 110 234 108 69 582 1497 187 2869
Fragments arranged by size:
2869 1497 582 234 187 110 108 69
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 2691 2693 1925
Size: 766 2 4888
Fragments arranged by size:
4888 766 2
BssSI C'ACGA_G
Cuts at: 2141 2897 3716 4728 2141
Size: 756 819 1012 3069
Fragments arranged by size:
3069 1012 819 756
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2314 2314
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2265 2284 2451 2986 3353 3738 3755 5207
Size: 293 19 167 535 367 385 17 1452
Cuts at: 5207 5293 5305 5651 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 535 385 367 346 293 167
86 19 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2011 513
Size: 552 946 4158
Fragments arranged by size:
4158 946 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2550 487
Size: 683 56 1324 3593
Fragments arranged by size:
3593 1324 683 56
BtsI GCAGTG_nn'
Cuts at: 2798 3172 3966 3986 2798
Size: 374 794 20 4468
Fragments arranged by size:
4468 794 374 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 3103 3992
Size: 54 1425 50 275 18 204 1069 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1069 889 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 3103 1537
Size: 275 222 1069 4090
Fragments arranged by size:
4090 1069 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2568 3586 1353
Size: 1215 1018 3423
Fragments arranged by size:
3423 1215 1018
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2621 4243 4617 4763 387
Size: 1064 138 1032 1622 374 146 1280
Fragments arranged by size:
1622 1280 1064 1032 374 146 138
EcoICRI GAG'CTC
Cuts at: 2487 2487
Size: 5656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2445 2592 2726 2744 3719 4316 5103
Size: 393 813 147 134 18 975 597 787
Cuts at: 5103 5371 1239
Size: 268 1524
Fragments arranged by size:
1524 975 813 787 597 393 268 147
134 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2933 2366
Size: 567 5089
EcoO109I rG'GnC_Cy
Cuts at: 2136 2136
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 2322 2620 2723 2747 2780 4803 1678
Size: 644 298 103 24 33 2023 2531
Fragments arranged by size:
2531 2023 644 298 103 33 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2471 2503 2528
Size: 32 135 32 548 32 319 32 25
Cuts at: 2528 2560 4297 4329 4371 4403 1373
Size: 32 1737 32 42 32 2626
Fragments arranged by size:
2626 1737 548 319 135 42 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KasI G'GCGC_C
Cuts at: 2318 2318
Size: 5656
KpnI G_GTAC'C
Cuts at: 2362 2981 2362
Size: 619 5037
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 2523 3614 3973 4132 518
Size: 648 1143 214 1091 359 159 2042
Fragments arranged by size:
2042 1143 1091 648 359 214 159
MspA1I CmG'CkG
Cuts at: 1606 2356 3072 3749 4897 5142 1606
Size: 750 716 677 1148 245 2120
Fragments arranged by size:
2120 1148 750 716 677 245
NaeI GCC'GGC
Cuts at: 2046 2046
Size: 5656
NarI GG'CG_CC
Cuts at: 2319 2319
Size: 5656
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NgoMIV G'CCGG_C
Cuts at: 2044 2044
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 2034 3103 2034
Size: 1069 4587
NspI r_CATG'y
Cuts at: 1132 1229 3014 4559 1132
Size: 97 1785 1545 2229
Fragments arranged by size:
2229 1785 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PfoI T'CCnGG_A
Cuts at: 2454 2454
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2136 2136
Size: 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2210 2210
Size: 5656
PspXI vC'TCGA_Gb
Cuts at: 2482 2482
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2340 2667 2751 3008 830
Size: 1510 327 84 257 3478
Fragments arranged by size:
3478 1510 327 257 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SacI G_AGCT'C
Cuts at: 2489 2489
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SanDI GG'GwC_CC
Cuts at: 2136 2136
Size: 5656
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SexAI A'CCwGG_T
Cuts at: 2952 2952
Size: 5656
SfcI C'TryA_G
Cuts at: 826 1045 2336 2663 2747 3004 4139 4820 5011
Size: 219 1291 327 84 257 1135 681 191
Cuts at: 5011 826
Size: 1471
Fragments arranged by size:
1471 1291 1135 681 327 257 219 191
84
SfoI GGC'GCC
Cuts at: 2320 2320
Size: 5656
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 2044 1700
Size: 344 5312
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2482 3760 4661 4923 820
Size: 197 926 539 1278 901 262 1553
Fragments arranged by size:
1553 1278 926 901 539 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2175 2214 3095 513
Size: 552 1110 39 881 3074
Fragments arranged by size:
3074 1110 881 552 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 3902 5614
Size: 274 80 33 51 1443 414 1511 1712
Cuts at: 5614 96
Size: 138
Fragments arranged by size:
1712 1511 1443 414 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2950 2950
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 2482 1943
Size: 539 5117
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PsrI
PvuII RsrII SacII SapI SfiI SrfI SwaI