Restriction map for
pFN21AE3670
(Circular) (Six-base) MAPSORT of: pFN21AE3670.seq Check: 9501 from: 1 to: 6184
pFN21AE3670 6184 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 1937
Size: 1590 4594
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2632 4247 5631 5899 1632
Size: 1000 1615 1384 268 1917
Fragments arranged by size:
1917 1615 1384 1000 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 2389 5083 6060 1203
Size: 1186 2694 977 1327
Fragments arranged by size:
2694 1327 1186 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 2418 1166
Size: 1252 4932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2957 1184
Size: 1773 4411
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 2168
Size: 32 6152
AlwNI CAG_nnn'CTG
Cuts at: 2496 3927 5499 2496
Size: 1431 1572 3181
Fragments arranged by size:
3181 1572 1431
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 3493 3719 3871 5980 1493
Size: 2000 226 152 2109 1697
Fragments arranged by size:
2109 2000 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 2364 3509 1870
Size: 73 228 193 1145 4545
Fragments arranged by size:
4545 1145 228 193 73
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 3505 4960 618
Size: 325 217 627 429 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 429 325 217
BanII G_rGCy'C
Cuts at: 1391 2737 3503 1391
Size: 1346 766 4072
Fragments arranged by size:
4072 1346 766
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 2761 3002 928
Size: 971 198 664 241 4110
Fragments arranged by size:
4110 971 664 241 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2730 2764 4375 4409 1903
Size: 34 793 34 1611 34 3678
Fragments arranged by size:
3678 1611 793 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BfrBI ATG'CAT
Cuts at: 3296 3433 3296
Size: 137 6047
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4795 136
Size: 107 122 71 631 605 3123 1525
Fragments arranged by size:
3123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 2242 3348 3601 2242
Size: 1106 253 4825
Fragments arranged by size:
4825 1106 253
Bme1580I G_kGCm'C
Cuts at: 1790 2221 2690 4245 5401 1790
Size: 431 469 1555 1156 2573
Fragments arranged by size:
2573 1555 1156 469 431
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3915 4873 442
Size: 416 53 83 399 24 2498 958 1753
Fragments arranged by size:
2498 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 3374 4844 1239
Size: 1424 711 1470 2579
Fragments arranged by size:
2579 1470 1424 711
Bpu10I CC'TnA_GC
Cuts at: 2559 2559
Size: 6184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3074 4309 5174 5472 3074
Size: 1235 865 298 3786
Fragments arranged by size:
3786 1235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4847 882
Size: 617 3348 2219
Fragments arranged by size:
3348 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 3975 493
Size: 711 918 1853 2702
Fragments arranged by size:
2702 1853 918 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2823 3311 4617 5015 5289 5436 5847 2823
Size: 488 1306 398 274 147 411 3160
Fragments arranged by size:
3160 1306 488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 1178
Size: 30 165 30 765 30 5164
Fragments arranged by size:
5164 765 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 3466 1186
Size: 1003 1277 3904
Fragments arranged by size:
3904 1277 1003
BseYI C'CCAG_C
Cuts at: 2496 5387 5883 2496
Size: 2891 496 2797
Fragments arranged by size:
2891 2797 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 2715 2865 3043 3414 1163
Size: 573 706 273 150 178 371 3933
Fragments arranged by size:
3933 706 573 371 273 178 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 2737 2881 4245 4330 5401 2232
Size: 505 144 1364 85 1071 3015
Fragments arranged by size:
3015 1364 1071 505 144 85
BsmI GAATG_Cn'
Cuts at: 3244 3695 3788 3244
Size: 451 93 5640
Fragments arranged by size:
5640 451 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2232 2690 2737 2881 3503 4245
Size: 399 431 11 458 47 144 622 742
Cuts at: 4245 4330 5401 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 622 458 431 399 144
85 47 11
BspEI T'CCGG_A
Cuts at: 2823 3311 5847 2823
Size: 488 2536 3160
Fragments arranged by size:
3160 2536 488
BspHI T'CATG_A
Cuts at: 3195 4073 5803 3195
Size: 878 1730 3576
Fragments arranged by size:
3576 1730 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3539 844
Size: 2695 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4071 1394
Size: 429 2248 3507
Fragments arranged by size:
3507 2248 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2047 2600 2951 4679 4853 65
Size: 1183 799 553 351 1728 174 1396
Fragments arranged by size:
1728 1396 1183 799 553 351 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4828 5015 1700
Size: 110 3018 187 2869
Fragments arranged by size:
3018 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 3173 4244 5256 3173
Size: 1071 1012 4101
Fragments arranged by size:
4101 1071 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2440 2757 2910 794
Size: 1646 317 153 4068
Fragments arranged by size:
4068 1646 317 153
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2140 3253 2011
Size: 129 1113 4942
Fragments arranged by size:
4942 1113 129
BstYI r'GATC_y
Cuts at: 1972 3514 3881 4266 4283 5735 5821 5833 6179
Size: 1542 367 385 17 1452 86 12 346
Cuts at: 6179 1972
Size: 1977
Fragments arranged by size:
1977 1542 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2706 513
Size: 552 1641 3991
Fragments arranged by size:
3991 1641 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2151 487
Size: 683 56 925 4520
Fragments arranged by size:
4520 925 683 56
BtsI GCAGTG_nn'
Cuts at: 2430 2506 3013 3700 4494 4514 2430
Size: 76 507 687 794 20 4100
Fragments arranged by size:
4100 794 687 507 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DraIII CAC_nnn'GTG
Cuts at: 2841 2841
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3255 3631 4520
Size: 54 1425 50 275 18 1425 376 889
Cuts at: 4520 6149 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1425 889 376 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 2067 2651 4114 1353
Size: 687 27 584 1463 3423
Fragments arranged by size:
3423 1463 687 584 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4771 5145 5291 387
Size: 1064 138 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2735 2735
Size: 6184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2632 2663 3374 4247 4844 5631 5899
Size: 393 1000 31 711 873 597 787 268
Cuts at: 5899 1239
Size: 1524
Fragments arranged by size:
1524 1000 873 787 711 597 393 268
31
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 6184
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 2641 5331 1678
Size: 963 2690 2531
Fragments arranged by size:
2690 2531 963
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4825 4857 4899 4931 1373
Size: 32 135 32 3253 32 42 32 2626
Fragments arranged by size:
3253 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3528 3774 669
Size: 988 281 1590 246 3079
Fragments arranged by size:
3079 1590 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3136 748
Size: 2388 3796
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5298 5482 1305
Size: 3993 184 2007
Fragments arranged by size:
3993 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3257 10
Size: 54 1425 343 1425 2937
Fragments arranged by size:
2937 1425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2418 3006 4142 4501 4660 518
Size: 648 843 409 588 1136 359 159 2042
Fragments arranged by size:
2042 1136 843 648 588 409 359 159
MspA1I CmG'CkG
Cuts at: 1606 2493 2683 3166 3352 3448 3600 4277 5425
Size: 887 190 483 186 96 152 677 1148
Cuts at: 5425 5670 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 887 677 483 245 190 186
152 96
NcoI C'CATG_G
Cuts at: 513 1065 2706 513
Size: 552 1641 3991
Fragments arranged by size:
3991 1641 552
NdeI CA'TA_TG
Cuts at: 387 3292 387
Size: 2905 3279
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NsiI A_TGCA'T
Cuts at: 3298 3435 3298
Size: 137 6047
NspI r_CATG'y
Cuts at: 1132 1229 2393 2944 3542 5087 1132
Size: 97 1164 551 598 1545 2229
Fragments arranged by size:
2229 1545 1164 598 551 97
PasI CC'CwG_GG
Cuts at: 1348 2099 1348
Size: 751 5433
PciI A'CATG_T
Cuts at: 2389 5083 2389
Size: 2694 3490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2168 2200 4253 4285 5766 5798 2168
Size: 32 2053 32 1481 32 2554
Fragments arranged by size:
2554 2053 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PspXI vC'TCGA_Gb
Cuts at: 2364 2364
Size: 6184
PstI C_TGCA'G
Cuts at: 830 3536 830
Size: 2706 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2493 2683 3352 2493
Size: 190 669 5325
Fragments arranged by size:
5325 669 190
SacI G_AGCT'C
Cuts at: 2737 2737
Size: 6184
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 3098 4432 6144 3098
Size: 1334 1712 3138
Fragments arranged by size:
3138 1712 1334
SfcI C'TryA_G
Cuts at: 826 1045 3233 3532 4667 5348 5539 826
Size: 219 2188 299 1135 681 191 1471
Fragments arranged by size:
2188 1471 1135 681 299 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 3511 1872
Size: 1639 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2364 3089 4288 5189 5451 820
Size: 197 926 421 725 1199 901 262 1553
Fragments arranged by size:
1553 1199 926 901 725 421 262 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6184
StyI C'CwwG_G
Cuts at: 513 1065 2706 3623 513
Size: 552 1641 917 3074
Fragments arranged by size:
3074 1641 917 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 4211 4228 4381 4566 5011 325
Size: 1255 1238 1393 17 153 185 445 1498
Fragments arranged by size:
1498 1393 1255 1238 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2743 3096 4430
Size: 274 80 33 51 1443 766 353 1334
Cuts at: 4430 6142 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1334 766 353 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2389 2421 6144 6176 2389
Size: 32 3723 32 2397
Fragments arranged by size:
3723 2397 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2687 2687
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 2364 1943
Size: 421 5763
XmaI C'CCGG_G
Cuts at: 1870 3509 1870
Size: 1639 4545
XmnI GAAnn'nnTTC
Cuts at: 3470 4313 3470
Size: 843 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI