Restriction map for
pFN21AE3666
(Circular) (Six-base) MAPSORT of: pFN21AE3666.seq Check: 9230 from: 1 to: 5770
pFN21AE3666 5770 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2365 2365
Size: 5770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2942 3833 5217 5485 1632
Size: 1310 891 1384 268 1917
Fragments arranged by size:
1917 1384 1310 891 268
AflII C'TTAA_G
Cuts at: 820 1017 2111 820
Size: 197 1094 4479
Fragments arranged by size:
4479 1094 197
AflIII A'CryG_T
Cuts at: 1203 2978 4669 5646 1203
Size: 1775 1691 977 1327
Fragments arranged by size:
1775 1691 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 2707 3513 5085 2707
Size: 806 1572 3392
Fragments arranged by size:
3392 1572 806
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 2788 3056 3079 3305 3457 5566 1493
Size: 1295 268 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 268 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 3095 1870
Size: 73 1152 4545
Fragments arranged by size:
4545 1152 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3091 4546 618
Size: 325 217 627 1304 1455 1842
Fragments arranged by size:
1842 1455 1304 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3089 1391
Size: 1698 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2208 928
Size: 971 309 4490
Fragments arranged by size:
4490 971 309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2280 2577 3662 4872 2280
Size: 297 1085 1210 3178
Fragments arranged by size:
3178 1210 1085 297
BclI T'GATC_A
Cuts at: 1548 2262 1548
Size: 714 5056
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2547 3831 4987 1790
Size: 757 1284 1156 2573
Fragments arranged by size:
2573 1284 1156 757
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2350 4430 1239
Size: 1111 2080 2579
Fragments arranged by size:
2579 2080 1111
Bpu10I CC'TnA_GC
Cuts at: 2282 2282
Size: 5770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3895 4760 5058 3895
Size: 865 298 4607
Fragments arranged by size:
4607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2359 4433 882
Size: 617 860 2074 2219
Fragments arranged by size:
2219 2074 860 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 2940 5764 2940
Size: 2824 2946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2339 2369 1178
Size: 30 165 30 624 30 282 30 4579
Fragments arranged by size:
4579 624 282 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2449 1186
Size: 862 401 4507
Fragments arranged by size:
4507 862 401
BseYI C'CCAG_C
Cuts at: 2125 4973 5469 2125
Size: 2848 496 2426
Fragments arranged by size:
2848 2426 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2624 3220 3981 5009 1540
Size: 275 186 623 596 761 1028 2301
Fragments arranged by size:
2301 1028 761 623 596 275 186
BsiHKAI G_wGCw'C
Cuts at: 3831 3916 4987 3831
Size: 85 1071 4614
Fragments arranged by size:
4614 1071 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2547 3089 3831 3916 4987 1391
Size: 399 757 542 742 85 1071 2174
Fragments arranged by size:
2174 1071 757 742 542 399 85
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2365 3125 844
Size: 1521 760 3489
Fragments arranged by size:
3489 1521 760
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2552 4414 4601 1700
Size: 110 199 543 1862 187 2869
Fragments arranged by size:
2869 1862 543 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 2099 1925
Size: 174 5596
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstXI CCAn_nnnn'nTGG
Cuts at: 2186 2186
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2404 2821 3100 3467 3852 3869 5321 5407
Size: 432 417 279 367 385 17 1452 86
Cuts at: 5407 5419 5765 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 432 417 385 367 346 279
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3050 3050
Size: 5770
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2179 513
Size: 552 939 24 151 4104
Fragments arranged by size:
4104 939 552 151 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2285 2476 487
Size: 683 56 1059 191 3781
Fragments arranged by size:
3781 1059 683 191 56
BtsI GCAGTG_nn'
Cuts at: 2644 3286 4080 4100 2644
Size: 642 794 20 4314
Fragments arranged by size:
4314 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 2758 3074 2758
Size: 316 5454
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2079 4777 809
Size: 507 763 2698 1802
Fragments arranged by size:
2698 1802 763 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2614 3217
Size: 54 1425 50 275 18 177 607 603
Cuts at: 3217 4106 5735 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 607 603 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2733 2826 3700 1353
Size: 1380 93 874 3423
Fragments arranged by size:
3423 1380 874 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2350 2942 3833 4430 5217 5485 1239
Size: 393 718 592 891 597 787 268 1524
Fragments arranged by size:
1524 891 787 718 597 592 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2704 2704
Size: 5770
EcoRI G'AATT_C
Cuts at: 2788 3056 3079 3457 2788
Size: 268 23 378 5101
Fragments arranged by size:
5101 378 268 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2546 2546
Size: 5770
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2339 2371 2901
Size: 32 135 32 455 32 280 32 530
Cuts at: 2901 2933 4411 4443 4485 4517 1373
Size: 32 1478 32 42 32 2626
Fragments arranged by size:
2626 1478 530 455 280 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2924 748
Size: 2176 3594
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 4884 5068 1305
Size: 1128 2451 184 2007
Fragments arranged by size:
2451 2007 1128 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2616 10
Size: 54 1425 343 784 3164
Fragments arranged by size:
3164 1425 784 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 3728 4087 4246 518
Size: 648 1083 1479 359 159 2042
Fragments arranged by size:
2042 1479 1083 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2449 3186 3863 5011 5256 1606
Size: 424 419 737 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 424 419 245
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 5770
NcoI C'CATG_G
Cuts at: 513 1065 2004 2179 513
Size: 552 939 175 4104
Fragments arranged by size:
4104 939 552 175
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2982 3128 4673 1132
Size: 97 1753 146 1545 2229
Fragments arranged by size:
2229 1753 1545 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 2978 4669 2978
Size: 1691 4079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2707 1108
Size: 1599 4171
PfoI T'CCnGG_A
Cuts at: 2415 2415
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2704 2704
Size: 5770
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 3122 830
Size: 2292 3478
PvuI CG_AT'CG
Cuts at: 2001 2624 2001
Size: 623 5147
PvuII CAG'CTG
Cuts at: 2449 2449
Size: 5770
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SexAI A'CCwGG_T
Cuts at: 2700 2700
Size: 5770
SfcI C'TryA_G
Cuts at: 826 1045 2985 3038 3118 4253 4934 5125 826
Size: 219 1940 53 80 1135 681 191 1471
Fragments arranged by size:
1940 1471 1135 681 219 191 80 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 3874 4775 5037 820
Size: 197 926 168 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197 168
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 2468 3001 3694 5
Size: 47 2416 533 693 2081
Fragments arranged by size:
2416 2081 693 533 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 2179 3209 513
Size: 552 939 175 1030 3074
Fragments arranged by size:
3074 1030 939 552 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2899 3797 3814 3967 4152 4597 325
Size: 1255 1319 898 17 153 185 445 1498
Fragments arranged by size:
1498 1319 1255 898 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2844 4016 5728
Size: 274 80 33 51 1443 867 1172 1712
Cuts at: 5728 96
Size: 138
Fragments arranged by size:
1712 1443 1172 867 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 2351 2702 1316
Size: 1035 351 4384
Fragments arranged by size:
4384 1035 351
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2374 2674 2374
Size: 300 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SfiI SfoI SrfI SwaI