Restriction map for
pFN21AE3661
(Circular) (Six-base) MAPSORT of: pFN21AE3661.seq Check: 6353 from: 1 to: 5968
pFN21AE3661 5968 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3105 3311 1937
Size: 1168 206 4594
Fragments arranged by size:
4594 1168 206
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2311 2822 2852 3102 4031 5415 5683 1632
Size: 679 511 30 250 929 1384 268 1917
Fragments arranged by size:
1917 1384 929 679 511 268 250 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 4867 5844 1203
Size: 3664 977 1327
Fragments arranged by size:
3664 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5968
AlwNI CAG_nnn'CTG
Cuts at: 2560 3711 5283 2560
Size: 1151 1572 3245
Fragments arranged by size:
3245 1572 1151
ApaI G_GGCC'C
Cuts at: 3035 3035
Size: 5968
ApaLI G'TGCA_C
Cuts at: 2642 4025 5181 2642
Size: 1383 1156 3429
Fragments arranged by size:
3429 1383 1156
ApoI r'AATT_y
Cuts at: 1493 3277 3503 3655 5764 1493
Size: 1784 226 152 2109 1697
Fragments arranged by size:
2109 1784 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 2679 3293 1870
Size: 73 736 614 4545
Fragments arranged by size:
4545 736 614 73
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2197 2981 3289 4744 618
Size: 325 217 627 410 784 308 1455 1842
Fragments arranged by size:
1842 1455 784 627 410 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2190 2885 2975 3035 3091 3287 1391
Size: 799 695 90 60 56 196 4072
Fragments arranged by size:
4072 799 695 196 90 60 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2317 2598 2627 928
Size: 971 418 281 29 4269
Fragments arranged by size:
4269 971 418 281 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2476 3860 5070 2476
Size: 1384 1210 3374
Fragments arranged by size:
3374 1384 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BfrBI ATG'CAT
Cuts at: 2491 2491
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4579 136
Size: 107 122 71 631 605 2907 1525
Fragments arranged by size:
2907 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3201 5963 3201
Size: 2762 3206
BlpI GC'TnA_GC
Cuts at: 2189 3231 3385 2189
Size: 1042 154 4772
Fragments arranged by size:
4772 1042 154
Bme1580I G_kGCm'C
Cuts at: 1790 2646 3035 4029 5185 1790
Size: 856 389 994 1156 2573
Fragments arranged by size:
2573 1156 994 856 389
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2283 2548 3699
Size: 416 53 83 399 24 866 265 1151
Cuts at: 3699 4657 442
Size: 958 1753
Fragments arranged by size:
1753 1151 958 866 416 399 265 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2813 4628 1239
Size: 1574 1815 2579
Fragments arranged by size:
2579 1815 1574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 4093 4958 5256 2645
Size: 1448 865 298 3357
Fragments arranged by size:
3357 1448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4631 882
Size: 617 3132 2219
Fragments arranged by size:
3132 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3759 493
Size: 711 2555 2702
Fragments arranged by size:
2702 2555 711
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3775 4157 4792
Size: 53 83 186 936 476 1766 382 635
Cuts at: 4792 275
Size: 1451
Fragments arranged by size:
1766 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3072 3217 4401 4799 5073 5220 5631 3072
Size: 145 1184 398 274 147 411 3409
Fragments arranged by size:
3409 1184 411 398 274 147 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2906 2936 2941 2971 1178
Size: 30 165 30 1503 30 5 30 4175
Fragments arranged by size:
4175 1503 165 30 30 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2005 2789 2909 1186
Size: 819 784 120 4245
Fragments arranged by size:
4245 819 784 120
BseYI C'CCAG_C
Cuts at: 2396 3191 5171 5667 2396
Size: 795 1980 496 2697
Fragments arranged by size:
2697 1980 795 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 2846 1163
Size: 573 390 720 4285
Fragments arranged by size:
4285 720 573 390
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2136 2443 3418 4179 5207 1540
Size: 275 186 135 307 975 761 1028 2301
Fragments arranged by size:
2301 1028 975 761 307 275 186 135
BsiHKAI G_wGCw'C
Cuts at: 2190 2646 4029 4114 5185 2190
Size: 456 1383 85 1071 2973
Fragments arranged by size:
2973 1383 1071 456 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2646 2885 2975 3035 3091 3287
Size: 399 400 456 239 90 60 56 196
Cuts at: 3287 4029 4114 5185 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 456 400 399 239 196
90 85 60 56
BspEI T'CCGG_A
Cuts at: 3072 3217 5631 3072
Size: 145 2414 3409
Fragments arranged by size:
3409 2414 145
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3323 844
Size: 2479 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2478 4463 4637 65
Size: 1183 1230 1985 174 1396
Fragments arranged by size:
1985 1396 1230 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4612 4799 1700
Size: 110 2802 187 2869
Fragments arranged by size:
2869 2802 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 4028 5040 4028
Size: 1012 4956
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 3164 794
Size: 1310 1060 3598
Fragments arranged by size:
3598 1310 1060
BstBI TT'CG_AA
Cuts at: 4772 4772
Size: 5968
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5968
BstYI r'GATC_y
Cuts at: 1972 3201 3298 3665 4050 4067 5519 5605 5617
Size: 1229 97 367 385 17 1452 86 12
Cuts at: 5617 5963 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1229 385 367 346 97 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2723 2723
Size: 5968
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2533 513
Size: 552 939 27 502 3948
Fragments arranged by size:
3948 939 552 502 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 2419 3484 4278 4298 2419
Size: 1065 794 20 4089
Fragments arranged by size:
4089 1065 794 20
ClaI AT'CG_AT
Cuts at: 1554 2344 1554
Size: 790 5178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 3415 4304
Size: 54 1425 50 275 18 303 1282 889
Cuts at: 4304 5933 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1282 889 303 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2133 3415 1537
Size: 275 321 1282 4090
Fragments arranged by size:
4090 1282 321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2766 2798 3898 1353
Size: 1413 32 1100 3423
Fragments arranged by size:
3423 1413 1100 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4555 4929 5075 387
Size: 1064 138 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2188 2188
Size: 5968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2813 2822 2852 3102 4031 4628
Size: 393 679 502 9 30 250 929 597
Cuts at: 4628 5415 5683 1239
Size: 787 268 1524
Fragments arranged by size:
1524 929 787 679 597 502 393 268
250 30 9
EcoO109I rG'GnC_Cy
Cuts at: 2054 2054
Size: 5968
EcoRI G'AATT_C
Cuts at: 3277 3655 3277
Size: 378 5590
EcoRV GAT'ATC
Cuts at: 3127 3127
Size: 5968
FspI TGC'GCA
Cuts at: 2615 4474 2615
Size: 1859 4109
HaeII r_GCGC'y
Cuts at: 1678 2906 5115 1678
Size: 1228 2209 2531
Fragments arranged by size:
2531 2209 1228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2906 2938 2941 2973 2994
Size: 32 135 32 1334 32 3 32 21
Cuts at: 2994 3026 3032 3064 4609 4641 4683 4715 1373
Size: 32 6 32 1545 32 42 32 2626
Fragments arranged by size:
2626 1545 1334 135 42 32 32 32
32 32 32 32 32 21 6 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2257 3312 3558 669
Size: 988 281 319 1055 246 3079
Fragments arranged by size:
3079 1055 988 319 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2529 5082 5266 1305
Size: 1224 2553 184 2007
Fragments arranged by size:
2553 2007 1224 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4146
Fragments arranged by size:
4146 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2606 3926 4285 4444 518
Size: 648 1440 1320 359 159 2042
Fragments arranged by size:
2042 1440 1320 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2830 3384 4061 5209 5454 1606
Size: 537 687 554 677 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 554 537 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2533 513
Size: 552 939 529 3948
Fragments arranged by size:
3948 939 552 529
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NsiI A_TGCA'T
Cuts at: 2493 2493
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 2491 2605 3326 4871 1132
Size: 97 1262 114 721 1545 2229
Fragments arranged by size:
2229 1545 1262 721 114 97
PasI CC'CwG_GG
Cuts at: 1348 3028 1348
Size: 1680 4288
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 1108
Size: 1452 4516
PfoI T'CCnGG_A
Cuts at: 3240 3240
Size: 5968
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2450 1613
Size: 837 5131
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PspOMI G'GGCC_C
Cuts at: 3031 3031
Size: 5968
PstI C_TGCA'G
Cuts at: 830 2945 3320 830
Size: 2115 375 3478
Fragments arranged by size:
3478 2115 375
PvuI CG_AT'CG
Cuts at: 2001 2443 2001
Size: 442 5526
PvuII CAG'CTG
Cuts at: 2830 2830
Size: 5968
SacI G_AGCT'C
Cuts at: 2190 2190
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SfcI C'TryA_G
Cuts at: 826 1045 2845 2941 3316 4451 5132 5323 826
Size: 219 1800 96 375 1135 681 191 1471
Fragments arranged by size:
1800 1471 1135 681 375 219 191 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 2681 3295 1872
Size: 809 614 4545
Fragments arranged by size:
4545 809 614
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2660 4072 4973 5235 820
Size: 197 926 717 1412 901 262 1553
Fragments arranged by size:
1553 1412 926 901 717 262 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 2787 3892 5
Size: 47 2735 1105 2081
Fragments arranged by size:
2735 2081 1105 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5968
StyI C'CwwG_G
Cuts at: 513 1065 2004 2533 3407 513
Size: 552 939 529 874 3074
Fragments arranged by size:
3074 939 874 552 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 2138 3995 4012 4165 4350 4795
Size: 1255 428 130 1857 17 153 185 445
Cuts at: 4795 325
Size: 1498
Fragments arranged by size:
1857 1498 1255 445 428 185 153 130
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2517 4214 5926
Size: 274 80 33 51 1443 540 1697 1712
Cuts at: 5926 96
Size: 138
Fragments arranged by size:
1712 1697 1443 540 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2323 2355 5928 5960 2323
Size: 32 3573 32 2331
Fragments arranged by size:
3573 2331 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5968
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 2679 3293 1870
Size: 809 614 4545
Fragments arranged by size:
4545 809 614
XmnI GAAnn'nnTTC
Cuts at: 2099 2123 4097 2099
Size: 24 1974 3970
Fragments arranged by size:
3970 1974 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI