Restriction map for
pFN21AE3660
(Circular) (Six-base) MAPSORT of: pFN21AE3660.seq Check: 4478 from: 1 to: 6040
pFN21AE3660 6040 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2496 2643 3077 3167 4103 5487 5755 1632
Size: 864 147 434 90 936 1384 268 1917
Fragments arranged by size:
1917 1384 936 864 434 268 147 90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 4939 5916 1203
Size: 3736 977 1327
Fragments arranged by size:
3736 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 2602 3090 3783 5355 2602
Size: 488 693 1572 3287
Fragments arranged by size:
3287 1572 693 488
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2661 4594 160
Size: 872 1629 1933 1606
Fragments arranged by size:
1933 1629 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 3117 3365 1870
Size: 73 1174 248 4545
Fragments arranged by size:
4545 1174 248 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3361 4816 618
Size: 325 217 627 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2433 2448 2796 2992 3041 3359 1391
Size: 1042 15 348 196 49 318 4072
Fragments arranged by size:
4072 1042 348 318 196 49 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3311 928
Size: 971 1412 3657
Fragments arranged by size:
3657 1412 971
BbvCI CC'TCA_GC
Cuts at: 3101 3101
Size: 6040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4231 4265 1903
Size: 34 50 34 2210 34 3678
Fragments arranged by size:
3678 2210 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 2769 1548
Size: 1221 4819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 2372 2987
Size: 107 122 71 631 605 646 54 615
Cuts at: 2987 4651 136
Size: 1664 1525
Fragments arranged by size:
1664 1525 646 631 615 605 122 107
71 54
BglII A'GATC_T
Cuts at: 6035 6035
Size: 6040
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2368 4101 5257 1790
Size: 578 1733 1156 2573
Fragments arranged by size:
2573 1733 1156 578
BmgBI CAC'GTC
Cuts at: 1318 3295 1318
Size: 1977 4063
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 2692 3771
Size: 416 53 83 399 24 697 578 1079
Cuts at: 3771 4729 442
Size: 958 1753
Fragments arranged by size:
1753 1079 958 697 578 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2213 1056
Size: 1157 4883
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4700 1239
Size: 3461 2579
Bpu10I CC'TnA_GC
Cuts at: 3101 3101
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4165 5030 5328 4165
Size: 865 298 4877
Fragments arranged by size:
4877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2184 4473 4871 5145 5292 5703 2184
Size: 2289 398 274 147 411 2521
Fragments arranged by size:
2521 2289 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 1178
Size: 30 165 30 771 30 5014
Fragments arranged by size:
5014 771 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2440 2441 3194 1186
Size: 1254 1 753 4032
Fragments arranged by size:
4032 1254 753 1
BseYI C'CCAG_C
Cuts at: 2193 3243 5243 5739 2193
Size: 1050 2000 496 2494
Fragments arranged by size:
2494 2000 1050 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2116 2705 1163
Size: 573 380 589 4498
Fragments arranged by size:
4498 589 573 380
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2433 2448 2757 4101 4186 5257 2433
Size: 15 309 1344 85 1071 3216
Fragments arranged by size:
3216 1344 1071 309 85 15
BsmI GAATG_Cn'
Cuts at: 2572 2822 3551 3644 2572
Size: 250 729 93 4968
Fragments arranged by size:
4968 729 250 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2807 2807
Size: 6040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2368 2433 2448 2757 2796 2992 3041
Size: 399 578 65 15 309 39 196 49
Cuts at: 3041 3359 4101 4186 5257 1391
Size: 318 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 399 318 309 196
85 65 49 39 15
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3258 3395 844
Size: 2414 137 3489
Fragments arranged by size:
3489 2414 137
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2553 4535 4709 65
Size: 1183 1305 1982 174 1396
Fragments arranged by size:
1982 1396 1305 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2994 96
Size: 2898 3142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2578 2602 3081 3090 3171 794
Size: 1784 24 479 9 81 3663
Fragments arranged by size:
3663 1784 479 81 24 9
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 2725 1184
Size: 1541 4499
BstYI r'GATC_y
Cuts at: 1972 2187 3370 3737 4122 4139 5591 5677 5689
Size: 215 1183 367 385 17 1452 86 12
Cuts at: 5689 6035 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1183 385 367 346 215 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 2406 3556 4350 4370 2358
Size: 48 1150 794 20 4028
Fragments arranged by size:
4028 1150 794 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 2764 3344 2764
Size: 580 5460
DraIII CAC_nnn'GTG
Cuts at: 3220 3222 3220
Size: 2 6038
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2691 2784 3211
Size: 54 1425 50 275 18 861 93 427
Cuts at: 3211 3487 4376 6005 8
Size: 276 889 1629 43
Fragments arranged by size:
1629 1425 889 861 427 276 275 93
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3970 1353
Size: 2617 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2431 2446 2431
Size: 15 6025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2496 2643 3077 3167 4103 4700 5487
Size: 393 864 147 434 90 936 597 787
Cuts at: 5487 5755 1239
Size: 268 1524
Fragments arranged by size:
1524 936 864 787 597 434 393 268
147 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 6040
EcoO109I rG'GnC_Cy
Cuts at: 2311 3097 2311
Size: 786 5254
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 6040
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 2853 5187 1678
Size: 1175 2334 2531
Fragments arranged by size:
2531 2334 1175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2094 2126 2659 2691 4681
Size: 32 135 32 522 32 533 32 1990
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1990 533 522 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 3384 3630 669
Size: 988 281 184 1262 246 3079
Fragments arranged by size:
3079 1262 988 281 246 184
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 2596 5154 5338 1305
Size: 1167 124 2558 184 2007
Fragments arranged by size:
2558 2007 1167 184 124
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2693 3213 10
Size: 54 1425 343 861 520 2837
Fragments arranged by size:
2837 1425 861 520 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3998 4357 4516 518
Size: 648 2832 359 159 2042
Fragments arranged by size:
2832 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2516 2790 3090 3105 3456 4133 5281 5526
Size: 910 274 300 15 351 677 1148 245
Cuts at: 5526 1606
Size: 2120
Fragments arranged by size:
2120 1148 910 677 351 300 274 245
15
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5488
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 2209 1052
Size: 1157 4883
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3287 3398 4943 1132
Size: 97 2058 111 1545 2229
Fragments arranged by size:
2229 2058 1545 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2200 1108
Size: 1092 4948
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 2139 3086 3129 3269 3392 830
Size: 1309 947 43 140 123 3478
Fragments arranged by size:
3478 1309 947 140 123 43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 2516 3090 3105 2516
Size: 574 15 5451
Fragments arranged by size:
5451 574 15
SacI G_AGCT'C
Cuts at: 2433 2448 2433
Size: 15 6025
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SexAI A'CCwGG_T
Cuts at: 2088 2088
Size: 6040
SfcI C'TryA_G
Cuts at: 826 1045 2135 2827 3082 3125 3248 3265 3388
Size: 219 1090 692 255 43 123 17 123
Cuts at: 3388 4523 5204 5395 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 692 681 255 219 191
123 123 43 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3119 3367 1872
Size: 1247 248 4545
Fragments arranged by size:
4545 1247 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4144 5045 5307 820
Size: 197 926 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2439 2730 2923 3479 513
Size: 552 1374 291 193 556 3074
Fragments arranged by size:
3074 1374 556 552 291 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2711 4067 4084 4237 4422 4867 325
Size: 1255 1131 1356 17 153 185 445 1498
Fragments arranged by size:
1498 1356 1255 1131 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2994 4286 5998
Size: 274 80 33 51 1443 1017 1292 1712
Cuts at: 5998 96
Size: 138
Fragments arranged by size:
1712 1443 1292 1017 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3187 3219 6000 6032 3187
Size: 32 2781 32 3195
Fragments arranged by size:
3195 2781 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2201 2384 3163 2201
Size: 183 779 5078
Fragments arranged by size:
5078 779 183
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3117 3365 1870
Size: 1247 248 4545
Fragments arranged by size:
4545 1247 248
XmnI GAAnn'nnTTC
Cuts at: 4169 4169
Size: 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI