Restriction map for
pFN21AE3659
(Circular) (Six-base) MAPSORT of: pFN21AE3659.seq Check: 5400 from: 1 to: 5230
pFN21AE3659 5230 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3040 278
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2573 1937
Size: 636 4594
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 4677 4945 1632
Size: 1661 1384 268 1917
Fragments arranged by size:
1917 1661 1384 268
AfeI AGC'GCT
Cuts at: 2076 2076
Size: 5230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 4129 5106 1203
Size: 2926 977 1327
Fragments arranged by size:
2926 1327 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 3959 3959
Size: 5230
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5230
AlwNI CAG_nnn'CTG
Cuts at: 2973 4545 2973
Size: 1572 3658
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2539 2765 2917 5026 1493
Size: 1046 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2555 1870
Size: 73 112 500 4545
Fragments arranged by size:
4545 500 112 73
AvrII C'CTAG_G
Cuts at: 2048 2048
Size: 5230
BamHI G'GATC_C
Cuts at: 2233 2560 2927 2233
Size: 327 367 4536
Fragments arranged by size:
4536 367 327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 4006 618
Size: 325 217 627 764 1455 1842
Fragments arranged by size:
1842 1455 764 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2549 1391
Size: 1158 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3421 3455 1903
Size: 34 1484 34 3678
Fragments arranged by size:
3678 1484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4332 3122
Size: 1210 4020
BclI T'GATC_A
Cuts at: 1548 2451 1548
Size: 903 4327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3841 136
Size: 107 122 71 631 605 2169 1525
Fragments arranged by size:
2169 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5225 5225
Size: 5230
BlpI GC'TnA_GC
Cuts at: 2130 2647 2130
Size: 517 4713
Bme1580I G_kGCm'C
Cuts at: 1790 3291 4447 1790
Size: 1501 1156 2573
Fragments arranged by size:
2573 1501 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2269 2961 3919
Size: 416 53 83 399 24 852 692 958
Cuts at: 3919 442
Size: 1753
Fragments arranged by size:
1753 958 852 692 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 3890 1239
Size: 1085 1566 2579
Fragments arranged by size:
2579 1566 1085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3355 4220 4518 3355
Size: 865 298 4067
Fragments arranged by size:
4067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3893 882
Size: 617 2394 2219
Fragments arranged by size:
2394 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3037 3419 4054 275
Size: 53 83 186 936 1504 382 635 1451
Fragments arranged by size:
1504 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3663 4061 4335 4482 4893 3663
Size: 398 274 147 411 4000
Fragments arranged by size:
4000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5005
Fragments arranged by size:
5005 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2078 2518 1186
Size: 889 3 440 3898
Fragments arranged by size:
3898 889 440 3
BseYI C'CCAG_C
Cuts at: 2095 4433 4929 2095
Size: 2338 496 2396
Fragments arranged by size:
2396 2338 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 1163
Size: 573 690 3967
Fragments arranged by size:
3967 690 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 2680 3441 4469 1540
Size: 275 186 68 611 761 1028 2301
Fragments arranged by size:
2301 1028 761 611 275 186 68
BsiHKAI G_wGCw'C
Cuts at: 2310 3291 3376 4447 2310
Size: 981 85 1071 3093
Fragments arranged by size:
3093 1071 981 85
BsmI GAATG_Cn'
Cuts at: 2741 2834 2741
Size: 93 5137
BsmBI CGTCTCn'nnnn_
Cuts at: 2048 2048
Size: 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2549 3291 3376 4447 1391
Size: 399 520 239 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 239 85
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 3119 4849 3119
Size: 1730 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2585 844
Size: 1741 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3117 1394
Size: 429 1294 3507
Fragments arranged by size:
3507 1294 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3725 3899 65
Size: 1183 2477 174 1396
Fragments arranged by size:
2477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2068 3874 4061 1700
Size: 110 220 38 1806 187 2869
Fragments arranged by size:
2869 1806 220 187 110 38
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5230
BssHII G'CGCG_C
Cuts at: 1925 2026 1925
Size: 101 5129
BssSI C'ACGA_G
Cuts at: 2117 3290 4302 2117
Size: 1173 1012 3045
Fragments arranged by size:
3045 1173 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5230
BstBI TT'CG_AA
Cuts at: 4034 4034
Size: 5230
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5230
BstXI CCAn_nnnn'nTGG
Cuts at: 2347 2347
Size: 5230
BstYI r'GATC_y
Cuts at: 1972 2233 2379 2560 2927 3312 3329 4781 4867
Size: 261 146 181 367 385 17 1452 86
Cuts at: 4867 4879 5225 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 261 181 146
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4491
Fragments arranged by size:
4491 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3540 3560 2746
Size: 794 20 4416
Fragments arranged by size:
4416 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5195
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 2445 2677
Size: 54 1425 50 275 18 236 379 232
Cuts at: 2677 3566 5195 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 379 275 236 232 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2066 2677 1537
Size: 275 254 611 4090
Fragments arranged by size:
4090 611 275 254
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 3160 1353
Size: 789 1018 3423
Fragments arranged by size:
3423 1018 789
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 3817 4191 4337 387
Size: 1064 138 480 1748 374 146 1280
Fragments arranged by size:
1748 1280 1064 480 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 3293 3890 4677 4945 1239
Size: 393 692 969 597 787 268 1524
Fragments arranged by size:
1524 969 787 692 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2052 2122 2052
Size: 70 5160
EcoRI G'AATT_C
Cuts at: 2539 2917 2539
Size: 378 4852
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 2078 2103 4377 1678
Size: 400 25 2274 2531
Fragments arranged by size:
2531 2274 400 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3871 3903 3945 3977 1373
Size: 32 135 32 2299 32 42 32 2626
Fragments arranged by size:
2626 2299 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2574 2820 669
Size: 988 281 636 246 3079
Fragments arranged by size:
3079 988 636 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5230
HpaI GTT'AAC
Cuts at: 2820 2820
Size: 5230
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4344 4528 1305
Size: 3039 184 2007
Fragments arranged by size:
3039 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2447 10
Size: 54 1425 343 615 2793
Fragments arranged by size:
2793 1425 615 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 3547 3706 518
Size: 648 2022 359 159 2042
Fragments arranged by size:
2042 2022 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2195 2646 3323 4471 4716 1606
Size: 589 451 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 451 245
NaeI GCC'GGC
Cuts at: 2032 2070 2032
Size: 38 5192
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NgoMIV G'CCGG_C
Cuts at: 2030 2068 2030
Size: 38 5192
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2677 2677
Size: 5230
NspI r_CATG'y
Cuts at: 1132 1229 2588 4133 1132
Size: 97 1359 1545 2229
Fragments arranged by size:
2229 1545 1359 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5230
PciI A'CATG_T
Cuts at: 4129 4129
Size: 5230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5230
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2052 2052
Size: 5230
PshAI GACnn'nnGTC
Cuts at: 1613 2057 2195 1613
Size: 444 138 4648
Fragments arranged by size:
4648 444 138
PsiI TTA'TAA
Cuts at: 2220 2800 2220
Size: 580 4650
PstI C_TGCA'G
Cuts at: 830 2582 830
Size: 1752 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
PvuII CAG'CTG
Cuts at: 2195 2195
Size: 5230
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SbfI CC_TGCA'GG
Cuts at: 2582 2582
Size: 5230
ScaI AGT'ACT
Cuts at: 3478 5190 3478
Size: 1712 3518
SfcI C'TryA_G
Cuts at: 826 1045 2578 3713 4394 4585 826
Size: 219 1533 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 2030 1700
Size: 330 4900
SmaI CCC'GGG
Cuts at: 1872 2557 1872
Size: 685 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3334 4235 4497 820
Size: 197 926 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5230
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 3154 5
Size: 47 3102 2081
Fragments arranged by size:
3102 2081 47
StuI AGG'CCT
Cuts at: 1517 2278 1517
Size: 761 4469
StyI C'CwwG_G
Cuts at: 513 1065 2004 2048 2346 2669 513
Size: 552 939 44 298 323 3074
Fragments arranged by size:
3074 939 552 323 298 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3257 3274 3427 3612 4057 325
Size: 1255 1677 17 153 185 445 1498
Fragments arranged by size:
1677 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2437 3476 5188
Size: 274 80 33 51 1443 460 1039 1712
Cuts at: 5188 96
Size: 138
Fragments arranged by size:
1712 1443 1039 460 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5190 5222 5190
Size: 32 5198
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5230
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2398
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2555 1870
Size: 685 4545
XmnI GAAnn'nnTTC
Cuts at: 3359 3359
Size: 5230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3038 276
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI