Restriction map for
pFN21AE3650
(Circular) (Six-base) MAPSORT of: pFN21AE3650.seq Check: 4895 from: 1 to: 6715
pFN21AE3650 6715 bp
With 182 enzymes: SgfI *
March 28, 2012 12:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3514 3514
Size: 6715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4525 278
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3468 4058 1937
Size: 1531 590 4594
Fragments arranged by size:
4594 1531 590
Acc65I G'GTAC_C
Cuts at: 4036 4036
Size: 6715
AclI AA'CG_TT
Cuts at: 1563 4842 5215 1563
Size: 3279 373 3063
Fragments arranged by size:
3279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2489 3927 4778 6162 6430 1632
Size: 857 1438 851 1384 268 1917
Fragments arranged by size:
1917 1438 1384 857 851 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6518
AflIII A'CryG_T
Cuts at: 1203 5614 6591 1203
Size: 4411 977 1327
Fragments arranged by size:
4411 1327 977
AgeI A'CCGG_T
Cuts at: 5546 5546
Size: 6715
AhdI GACnn_n'nnGTC
Cuts at: 5444 5444
Size: 6715
AleI CACnn'nnGTG
Cuts at: 1166 3557 1166
Size: 2391 4324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6715
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2021 2053 2021
Size: 32 6683
AlwNI CAG_nnn'CTG
Cuts at: 3436 4458 6030 3436
Size: 1022 1572 4121
Fragments arranged by size:
4121 1572 1022
ApaI G_GGCC'C
Cuts at: 2660 2660
Size: 6715
ApaLI G'TGCA_C
Cuts at: 4772 5928 4772
Size: 1156 5559
ApoI r'AATT_y
Cuts at: 1493 4024 4250 4402 6511 1493
Size: 2531 226 152 2109 1697
Fragments arranged by size:
2531 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5269 160
Size: 872 4237 1606
Fragments arranged by size:
4237 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2321 3206 3311 4040 1870
Size: 73 81 297 885 105 729 4545
Fragments arranged by size:
4545 885 729 297 105 81 73
BamHI G'GATC_C
Cuts at: 4045 4412 4045
Size: 367 6348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2644 2686 3024 3489
Size: 325 217 627 309 548 42 338 465
Cuts at: 3489 4036 5491 618
Size: 547 1455 1842
Fragments arranged by size:
1842 1455 627 548 547 465 338 325
309 217 42
BanII G_rGCy'C
Cuts at: 1391 2303 2515 2660 3126 3144 3207 3273 3411
Size: 912 212 145 466 18 63 66 138
Cuts at: 3411 4034 1391
Size: 623 4072
Fragments arranged by size:
4072 912 623 466 212 145 138 66
63 18
BbeI G_GCGC'C
Cuts at: 2100 2648 2690 3028 2100
Size: 548 42 338 5787
Fragments arranged by size:
5787 548 338 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2483 2603 3915 928
Size: 971 584 120 1312 3728
Fragments arranged by size:
3728 1312 971 584 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4906 4940 1903
Size: 34 50 34 2885 34 3678
Fragments arranged by size:
3678 2885 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4607 5817 4607
Size: 1210 5505
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6715
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2324 2519 2869
Size: 107 122 71 631 605 652 195 350
Cuts at: 2869 2890 5326 136
Size: 21 2436 1525
Fragments arranged by size:
2436 1525 652 631 605 350 195 122
107 71 21
BglII A'GATC_T
Cuts at: 6710 6710
Size: 6715
BlpI GC'TnA_GC
Cuts at: 3090 4132 3090
Size: 1042 5673
Bme1580I G_kGCm'C
Cuts at: 1790 2660 3982 4776 5932 1790
Size: 870 1322 794 1156 2573
Fragments arranged by size:
2573 1322 1156 870 794
BmgBI CAC'GTC
Cuts at: 1318 3481 3559 1318
Size: 2163 78 4474
Fragments arranged by size:
4474 2163 78
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3800 4446 5404
Size: 416 53 83 399 24 2383 646 958
Cuts at: 5404 442
Size: 1753
Fragments arranged by size:
2383 1753 958 646 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6715
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2587 2676 3391 2449
Size: 138 89 715 5773
Fragments arranged by size:
5773 715 138 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2509 2890 3005 3239 3527 5375 1239
Size: 1270 381 115 234 288 1848 2579
Fragments arranged by size:
2579 1848 1270 381 288 234 115
Bpu10I CC'TnA_GC
Cuts at: 2739 3125 2739
Size: 386 6329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3244 3626 4840 5705 6003 3244
Size: 382 1214 865 298 3956
Fragments arranged by size:
3956 1214 865 382 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2506 5378 882
Size: 617 1007 2872 2219
Fragments arranged by size:
2872 2219 1007 617
BsaAI yAC'GTr
Cuts at: 493 1204 4506 493
Size: 711 3302 2702
Fragments arranged by size:
3302 2702 711
BsaBI GATnn'nnATC
Cuts at: 6709 6709
Size: 6715
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2480 2645 2687
Size: 53 83 186 936 564 383 165 42
Cuts at: 2687 2765 3025 4522 4904 5539 275
Size: 78 260 1497 382 635 1451
Fragments arranged by size:
1497 1451 936 635 564 383 382 260
186 165 83 78 53 42
BsaWI w'CCGG_w
Cuts at: 5148 5546 5820 5967 6378 5148
Size: 398 274 147 411 5485
Fragments arranged by size:
5485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2693 2723 1178
Size: 30 165 30 1290 30 5170
Fragments arranged by size:
5170 1290 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2293 2579 2761 2764 2767 2776 2779 2782
Size: 1107 286 182 3 3 9 3 3
Cuts at: 2782 3026 3029 3062 3181 3329 3434 3521 3734
Size: 244 3 33 119 148 105 87 213
Cuts at: 3734 3752 3767 3878 3892 3896 4022 1186
Size: 18 15 111 14 4 126 3879
Fragments arranged by size:
3879 1107 286 244 213 182 148 126
119 111 105 87 33 18 15 14
9 4 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3511 3695 3785 5918 6414 3511
Size: 184 90 2133 496 3812
Fragments arranged by size:
3812 2133 496 184 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3422 3698 3899 1163
Size: 573 1686 276 201 3979
Fragments arranged by size:
3979 1686 573 276 201
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2762 3079 3961 4165 4926 5954
Size: 275 186 761 317 882 204 761 1028
Cuts at: 5954 1540
Size: 2301
Fragments arranged by size:
2301 1028 882 761 761 317 275 204
186
BsiHKAI G_wGCw'C
Cuts at: 2303 3126 3861 4776 4861 5932 2303
Size: 823 735 915 85 1071 3086
Fragments arranged by size:
3086 1071 915 823 735 85
BsiWI C'GTAC_G
Cuts at: 3922 3922
Size: 6715
BsmI GAATG_Cn'
Cuts at: 3502 4226 4319 3502
Size: 724 93 5898
Fragments arranged by size:
5898 724 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2303 2515 2660 3126 3144 3207 3273
Size: 399 513 212 145 466 18 63 66
Cuts at: 3273 3411 3861 3982 4034 4776 4861 5932 1391
Size: 138 450 121 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 513 466 450 399 212
145 138 121 85 66 63 52 18
BspEI T'CCGG_A
Cuts at: 6378 6378
Size: 6715
BspHI T'CATG_A
Cuts at: 4604 6334 4604
Size: 1730 4985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3514 3921 4070 844
Size: 2670 407 149 3489
Fragments arranged by size:
3489 2670 407 149
BsrBI CCG'CTC
Cuts at: 1394 1823 3058 3070 3094 3349 4602 1394
Size: 429 1235 12 24 255 1253 3507
Fragments arranged by size:
3507 1253 1235 429 255 24 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 5210 5384 65
Size: 1183 3962 174 1396
Fragments arranged by size:
3962 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2743 3960 5359 5546 1700
Size: 110 933 1217 1399 187 2869
Fragments arranged by size:
2869 1399 1217 933 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6715
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6715
BssSI C'ACGA_G
Cuts at: 3860 4775 5787 3860
Size: 915 1012 4788
Fragments arranged by size:
4788 1012 915
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3436 794
Size: 2642 4073
BstBI TT'CG_AA
Cuts at: 5519 5519
Size: 6715
BstEII G'GTnAC_C
Cuts at: 1184 2395 3526 1184
Size: 1211 1131 4373
Fragments arranged by size:
4373 1211 1131
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 6715
BstYI r'GATC_y
Cuts at: 1972 2253 3537 4045 4412 4797 4814 6266 6352
Size: 281 1284 508 367 385 17 1452 86
Cuts at: 6352 6364 6710 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1284 508 385 367 346 281
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2648 2751 3079 3414 513
Size: 552 1583 103 328 335 3814
Fragments arranged by size:
3814 1583 552 335 328 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2071 487
Size: 683 56 845 5131
Fragments arranged by size:
5131 845 683 56
BtsI GCAGTG_nn'
Cuts at: 2350 2917 4231 5025 5045 2350
Size: 567 1314 794 20 4020
Fragments arranged by size:
4020 1314 794 567 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6680
DraI TTT'AAA
Cuts at: 4019 4019
Size: 6715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2375 5722 809
Size: 507 1059 3347 1802
Fragments arranged by size:
3347 1802 1059 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2748 3286
Size: 54 1425 50 275 18 552 366 538
Cuts at: 3286 3564 3729 3744 4162 5051 6680 8
Size: 278 165 15 418 889 1629 43
Fragments arranged by size:
1629 1425 889 552 538 418 366 278
275 165 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4162 1537
Size: 275 2350 4090
Fragments arranged by size:
4090 2350 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2465 2556 2785 3293 3479 4645 1353
Size: 1112 91 229 508 186 1166 3423
Fragments arranged by size:
3423 1166 1112 508 229 186 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2280 2887 3788 5302 5676 5822
Size: 1064 138 691 607 901 1514 374 146
Cuts at: 5822 387
Size: 1280
Fragments arranged by size:
1514 1280 1064 901 691 607 374 146
138
EcoICRI GAG'CTC
Cuts at: 2301 3124 2301
Size: 823 5892
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 2509 2890 3005 3239 3527 3927
Size: 393 857 20 381 115 234 288 400
Cuts at: 3927 4778 5375 6162 6430 1239
Size: 851 597 787 268 1524
Fragments arranged by size:
1524 857 851 787 597 400 393 381
288 268 234 115 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2537 4004 2537
Size: 1467 5248
EcoO109I rG'GnC_Cy
Cuts at: 2165 2173 2317 2656 2657 3179 3652 3757 3932
Size: 8 144 339 1 522 473 105 175
Cuts at: 3932 3997 2165
Size: 65 4883
Fragments arranged by size:
4883 522 473 339 175 144 105 65
8 1
EcoRI G'AATT_C
Cuts at: 4024 4402 4024
Size: 378 6337
FspI TGC'GCA
Cuts at: 5221 5221
Size: 6715
HaeII r_GCGC'y
Cuts at: 1678 2100 2286 2648 2690 3028 5862 1678
Size: 422 186 362 42 338 2834 2531
Fragments arranged by size:
2834 2531 422 362 338 186 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 2693 2725 3809
Size: 32 135 32 851 32 238 32 1084
Cuts at: 3809 3841 5356 5388 5430 5462 1373
Size: 32 1515 32 42 32 2626
Fragments arranged by size:
2626 1515 1084 851 238 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3469 4059 4305 669
Size: 988 281 1531 590 246 3079
Fragments arranged by size:
3079 1531 988 590 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6715
HpaI GTT'AAC
Cuts at: 4305 4305
Size: 6715
KasI G'GCGC_C
Cuts at: 2096 2644 2686 3024 2096
Size: 548 42 338 5787
Fragments arranged by size:
5787 548 338 42
KpnI G_GTAC'C
Cuts at: 4040 4040
Size: 6715
MfeI C'AATT_G
Cuts at: 4314 4314
Size: 6715
MluI A'CGCG_T
Cuts at: 6591 6591
Size: 6715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 2571 2724 2730 2820 5829 6013 1305
Size: 1026 240 153 6 90 3009 184 2007
Fragments arranged by size:
3009 2007 1026 240 184 153 90 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2750 3288 3566 10
Size: 54 1425 343 918 538 278 3159
Fragments arranged by size:
3159 1425 918 538 343 278 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3557 4673 5032 5191 518
Size: 648 2391 1116 359 159 2042
Fragments arranged by size:
2391 2042 1116 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2393 2408 2636 2843 3081 3102 3114 4131
Size: 787 15 228 207 238 21 12 1017
Cuts at: 4131 4808 5956 6201 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1017 787 677 245 238 228
207 21 15 12
NarI GG'CG_CC
Cuts at: 2097 2645 2687 3025 2097
Size: 548 42 338 5787
Fragments arranged by size:
5787 548 338 42
NcoI C'CATG_G
Cuts at: 513 1065 2751 513
Size: 552 1686 4477
Fragments arranged by size:
4477 1686 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6715
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6715
NotI GC'GGCC_GC
Cuts at: 4162 4162
Size: 6715
NspI r_CATG'y
Cuts at: 1132 1229 4073 5618 1132
Size: 97 2844 1545 2229
Fragments arranged by size:
2844 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6715
PciI A'CATG_T
Cuts at: 5614 5614
Size: 6715
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3812 3896 1108
Size: 2704 84 3927
Fragments arranged by size:
3927 2704 84
PmeI GTTT'AAAC
Cuts at: 4019 4019
Size: 6715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4784 4816 6297 6329 4784
Size: 32 1481 32 5170
Fragments arranged by size:
5170 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2165 2173 3179 3652 3932 2165
Size: 8 1006 473 280 4948
Fragments arranged by size:
4948 1006 473 280 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6715
PsiI TTA'TAA
Cuts at: 4285 4285
Size: 6715
PspOMI G'GGCC_C
Cuts at: 2656 2656
Size: 6715
PstI C_TGCA'G
Cuts at: 830 3432 3525 4067 830
Size: 2602 93 542 3478
Fragments arranged by size:
3478 2602 542 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6715
PvuII CAG'CTG
Cuts at: 2408 2408
Size: 6715
RsrII CG'GwC_CG
Cuts at: 2638 3363 2638
Size: 725 5990
SacI G_AGCT'C
Cuts at: 2303 3126 2303
Size: 823 5892
SacII CC_GC'GG
Cuts at: 3082 3082
Size: 6715
SalI G'TCGA_C
Cuts at: 1936 3467 4057 1936
Size: 1531 590 4594
Fragments arranged by size:
4594 1531 590
SanDI GG'GwC_CC
Cuts at: 2165 3179 3932 2165
Size: 1014 753 4948
Fragments arranged by size:
4948 1014 753
SbfI CC_TGCA'GG
Cuts at: 3525 4067 3525
Size: 542 6173
ScaI AGT'ACT
Cuts at: 4963 6675 4963
Size: 1712 5003
SfcI C'TryA_G
Cuts at: 826 1045 2278 3428 3521 4063 5198 5879 6070
Size: 219 1233 1150 93 542 1135 681 191
Cuts at: 6070 826
Size: 1471
Fragments arranged by size:
1471 1233 1150 1135 681 542 219 191
93
SfiI GGCCn_nnn'nGGCC
Cuts at: 2324 2324
Size: 6715
SfoI GGC'GCC
Cuts at: 2098 2646 2688 3026 2098
Size: 548 42 338 5787
Fragments arranged by size:
5787 548 338 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6715
SmaI CCC'GGG
Cuts at: 1872 2026 4042 1872
Size: 154 2016 4545
Fragments arranged by size:
4545 2016 154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3259 3641 4819 5720 5982 820
Size: 197 926 1316 382 1178 901 262 1553
Fragments arranged by size:
1553 1316 1178 926 901 382 262 197
SnaBI TAC'GTA
Cuts at: 493 4506 493
Size: 4013 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6715
SphI G_CATG'C
Cuts at: 1132 4073 1132
Size: 2941 3774
SspI AAT'ATT
Cuts at: 5 52 4639 5
Size: 47 4587 2081
Fragments arranged by size:
4587 2081 47
StuI AGG'CCT
Cuts at: 1517 2522 3949 1517
Size: 1005 1427 4283
Fragments arranged by size:
4283 1427 1005
StyI C'CwwG_G
Cuts at: 513 1065 2169 2712 2751 3890 4154 513
Size: 552 1104 543 39 1139 264 3074
Fragments arranged by size:
3074 1139 1104 552 543 264 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2128 2488 4742 4759 4912 5097 5542
Size: 1255 548 360 2254 17 153 185 445
Cuts at: 5542 325
Size: 1498
Fragments arranged by size:
2254 1498 1255 548 445 360 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4961 6673 96
Size: 274 80 33 51 1443 2984 1712 138
Fragments arranged by size:
2984 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6675 6707 6675
Size: 32 6683
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6715
XbaI T'CTAG_A
Cuts at: 4051 4051
Size: 6715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2419 2419
Size: 6715
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6715
XmaI C'CCGG_G
Cuts at: 1870 2024 4040 1870
Size: 154 2016 4545
Fragments arranged by size:
4545 2016 154
XmnI GAAnn'nnTTC
Cuts at: 2231 4844 2231
Size: 2613 4102
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4523 276
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsmBI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI SapI
SexAI SrfI SwaI