Restriction map for
pFN21AE3649
(Circular) (Six-base) MAPSORT of: pFN21AE3649.seq Check: 6825 from: 1 to: 6016
pFN21AE3649 6016 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3101 3101
Size: 6016
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 1937
Size: 1422 4594
Acc65I G'GTAC_C
Cuts at: 2728 3337 2728
Size: 609 5407
AclI AA'CG_TT
Cuts at: 1563 2800 4143 4516 1563
Size: 1237 1343 373 3063
Fragments arranged by size:
3063 1343 1237 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2164 2522 2576 4079 5463 5731 1632
Size: 532 358 54 1503 1384 268 1917
Fragments arranged by size:
1917 1503 1384 532 358 268 54
AflII C'TTAA_G
Cuts at: 820 1017 3281 820
Size: 197 2264 3555
Fragments arranged by size:
3555 2264 197
AflIII A'CryG_T
Cuts at: 1203 4915 5892 1203
Size: 3712 977 1327
Fragments arranged by size:
3712 1327 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 2392 3007 3305 3759 5331 2392
Size: 615 298 454 1572 3077
Fragments arranged by size:
3077 1572 615 454 298
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 3325 3551 3703 5812 1493
Size: 1832 226 152 2109 1697
Fragments arranged by size:
2109 1832 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 3341 1870
Size: 73 144 1254 4545
Fragments arranged by size:
4545 1254 144 73
BamHI G'GATC_C
Cuts at: 2322 3346 3713 2322
Size: 1024 367 4625
Fragments arranged by size:
4625 1024 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 2728 3337 4792 618
Size: 325 217 627 708 233 609 1455 1842
Fragments arranged by size:
1842 1455 708 627 609 325 233 217
BanII G_rGCy'C
Cuts at: 1391 2411 2925 3335 1391
Size: 1020 514 410 4072
Fragments arranged by size:
4072 1020 514 410
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2170 928
Size: 971 271 4774
Fragments arranged by size:
4774 971 271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2454 3908 5118 2454
Size: 1454 1210 3352
Fragments arranged by size:
3352 1454 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BfrBI ATG'CAT
Cuts at: 2480 2584 2480
Size: 104 5912
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3108 4627 136
Size: 107 122 71 631 605 1436 1519 1525
Fragments arranged by size:
1525 1519 1436 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6011 6011
Size: 6016
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 2678 3041 3174 4077 5233 1790
Size: 888 363 133 903 1156 2573
Fragments arranged by size:
2573 1156 903 888 363 133
BmgBI CAC'GTC
Cuts at: 1318 2136 1318
Size: 818 5198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2716 3194 3747
Size: 416 53 83 399 24 1299 478 553
Cuts at: 3747 4705 442
Size: 958 1753
Fragments arranged by size:
1753 1299 958 553 478 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2414 2643 4676 1239
Size: 1175 229 2033 2579
Fragments arranged by size:
2579 2033 1175 229
Bpu10I CC'TnA_GC
Cuts at: 2972 3104 2972
Size: 132 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 4141 5006 5304 2774
Size: 1367 865 298 3486
Fragments arranged by size:
3486 1367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4679 882
Size: 617 3180 2219
Fragments arranged by size:
3180 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2583 2613 1178
Size: 30 165 30 1180 30 4581
Fragments arranged by size:
4581 1180 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2442 2516 3290 1186
Size: 1256 74 774 3912
Fragments arranged by size:
3912 1256 774 74
BseYI C'CCAG_C
Cuts at: 3123 5219 5715 3123
Size: 2096 496 3424
Fragments arranged by size:
3424 2096 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 2933 1163
Size: 573 576 621 4246
Fragments arranged by size:
4246 621 576 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 4077 4162 5233 2094
Size: 1983 85 1071 2877
Fragments arranged by size:
2877 1983 1071 85
BsmI GAATG_Cn'
Cuts at: 3527 3620 3527
Size: 93 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2411 2678 2925 3041 3174 3335
Size: 399 304 317 267 247 116 133 161
Cuts at: 3335 4077 4162 5233 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 317 304 267 247
161 133 116 85
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 3905 5635 3905
Size: 1730 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 3371 844
Size: 2257 270 3489
Fragments arranged by size:
3489 2257 270
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 3108 3903 1394
Size: 429 281 1004 795 3507
Fragments arranged by size:
3507 1004 795 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 4511 4685 65
Size: 1183 3263 174 1396
Fragments arranged by size:
3263 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2841 4660 4847 1700
Size: 110 1031 1819 187 2869
Fragments arranged by size:
2869 1819 1031 187 110
BsrGI T'GTAC_A
Cuts at: 96 2073 3204 96
Size: 1977 1131 2908
Fragments arranged by size:
2908 1977 1131
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2477 2621 3007 3305 794
Size: 1683 144 386 298 3505
Fragments arranged by size:
3505 1683 386 298 144
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 2960 1184
Size: 1776 4240
BstYI r'GATC_y
Cuts at: 1972 2166 2322 3346 3713 4098 4115 5567 5653
Size: 194 156 1024 367 385 17 1452 86
Cuts at: 5653 5665 6011 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1024 385 367 346 194 156
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2306 2746 513
Size: 552 939 302 440 3783
Fragments arranged by size:
3783 939 552 440 302
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 2676 2945 3532 4326 4346 2676
Size: 269 587 794 20 4346
Fragments arranged by size:
4346 794 587 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DraIII CAC_nnn'GTG
Cuts at: 3202 3202
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2772 3463 4352
Size: 54 1425 50 275 18 942 691 889
Cuts at: 4352 5981 8
Size: 1629 43
Fragments arranged by size:
1629 1425 942 889 691 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2810 3946 1353
Size: 1457 1136 3423
Fragments arranged by size:
3423 1457 1136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2164 2414 2522 2576 2643 4079 4676
Size: 393 532 250 108 54 67 1436 597
Cuts at: 4676 5463 5731 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1436 787 597 532 393 268 250
108 67 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2669 2669
Size: 6016
EcoO109I rG'GnC_Cy
Cuts at: 2432 3004 2432
Size: 572 5444
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 5163 1678
Size: 3485 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2980 3012 3278 3310 4657
Size: 32 135 32 1408 32 266 32 1347
Cuts at: 4657 4689 4731 4763 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1408 1347 266 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3360 3606 669
Size: 988 281 1422 246 3079
Fragments arranged by size:
3079 1422 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2531 748
Size: 1783 4233
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KpnI G_GTAC'C
Cuts at: 2732 3341 2732
Size: 609 5407
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2569 5130 5314 1305
Size: 1264 2561 184 2007
Fragments arranged by size:
2561 2007 1264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4194
Fragments arranged by size:
4194 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3023 3974 4333 4492 518
Size: 648 1857 951 359 159 2042
Fragments arranged by size:
2042 1857 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2877 3138 3432 4109 5257 5502 1606
Size: 465 806 261 294 677 1148 245 2120
Fragments arranged by size:
2120 1148 806 677 465 294 261 245
NaeI GCC'GGC
Cuts at: 2843 2843
Size: 6016
NcoI C'CATG_G
Cuts at: 513 1065 2004 2306 2746 513
Size: 552 939 302 440 3783
Fragments arranged by size:
3783 939 552 440 302
NdeI CA'TA_TG
Cuts at: 387 2464 387
Size: 2077 3939
NgoMIV G'CCGG_C
Cuts at: 2841 2841
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NsiI A_TGCA'T
Cuts at: 2482 2586 2482
Size: 104 5912
NspI r_CATG'y
Cuts at: 1132 1229 2588 3374 4919 1132
Size: 97 1359 786 1545 2229
Fragments arranged by size:
2229 1545 1359 786 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2368 2417 1108
Size: 1260 49 4707
Fragments arranged by size:
4707 1260 49
PfoI T'CCnGG_A
Cuts at: 2391 2391
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2817 4085 4117 5598 5630 2785
Size: 32 1268 32 1481 32 3171
Fragments arranged by size:
3171 1481 1268 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2432 2432
Size: 6016
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PstI C_TGCA'G
Cuts at: 830 3012 3189 3368 830
Size: 2182 177 179 3478
Fragments arranged by size:
3478 2182 179 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SapI GCTCTTCn'nnn_
Cuts at: 2810 2810
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3012 3368 3012
Size: 356 5660
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2373 3008 3185 3251 3364 4499 5180
Size: 219 1328 635 177 66 113 1135 681
Cuts at: 5180 5371 826
Size: 191 1471
Fragments arranged by size:
1471 1328 1135 681 635 219 191 177
113 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2789 3281 4120 5021 5283 820
Size: 197 926 846 492 839 901 262 1553
Fragments arranged by size:
1553 926 901 846 839 492 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 2259 2507 1517
Size: 742 248 5026
Fragments arranged by size:
5026 742 248
StyI C'CwwG_G
Cuts at: 513 1065 2004 2210 2306 2746 2956 3455 513
Size: 552 939 206 96 440 210 499 3074
Fragments arranged by size:
3074 939 552 499 440 210 206 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2473 2821 3155 3213 4043 4060 4213
Size: 1255 893 348 334 58 830 17 153
Cuts at: 4213 4398 4843 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 893 830 445 348 334 185
153 58 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 2547 3015
Size: 274 80 33 51 1443 96 474 468
Cuts at: 3015 3204 4262 5974 96
Size: 189 1058 1712 138
Fragments arranged by size:
1712 1443 1058 474 468 274 189 138
96 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 5976 6008 2583
Size: 32 3361 32 2591
Fragments arranged by size:
3361 2591 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2436 1316
Size: 1120 4896
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 2237 4145 2237
Size: 1908 4108
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI