Restriction map for
pFN21AE3648
(Circular) (Six-base) MAPSORT of: pFN21AE3648.seq Check: 7292 from: 1 to: 5407
pFN21AE3648 5407 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2437 2437
Size: 5407
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2063 3217 278
Size: 53 83 186 936 527 1154 2468
Fragments arranged by size:
2468 1154 936 527 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 3470 4854 5122 1632
Size: 521 1317 1384 268 1917
Fragments arranged by size:
1917 1384 1317 521 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 4306 5283 1203
Size: 3103 977 1327
Fragments arranged by size:
3103 1327 977
AgeI A'CCGG_T
Cuts at: 2283 4238 2283
Size: 1955 3452
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2045 1184
Size: 861 4546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 2270
Size: 32 5375
AlwNI CAG_nnn'CTG
Cuts at: 2266 3150 4722 2266
Size: 884 1572 2951
Fragments arranged by size:
2951 1572 884
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2732 1870
Size: 73 789 4545
Fragments arranged by size:
4545 789 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2437 2608 2728 4183
Size: 325 217 627 333 317 171 120 1455
Cuts at: 4183 618
Size: 1842
Fragments arranged by size:
1842 1455 627 333 325 317 217 171
120
BanII G_rGCy'C
Cuts at: 1391 2204 2726 1391
Size: 813 522 4072
Fragments arranged by size:
4072 813 522
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 5407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2063 928
Size: 971 164 4272
Fragments arranged by size:
4272 971 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 136
Size: 107 122 71 631 605 2346 1525
Fragments arranged by size:
2346 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2690 5402 2690
Size: 2712 2695
BlpI GC'TnA_GC
Cuts at: 2529 2824 2529
Size: 295 5112
Bme1580I G_kGCm'C
Cuts at: 1790 2440 3468 4624 1790
Size: 650 1028 1156 2573
Fragments arranged by size:
2573 1156 1028 650
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2477 4067 1239
Size: 1058 180 1590 2579
Fragments arranged by size:
2579 1590 1058 180
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 5407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2474 3532 4397 4695 2474
Size: 1058 865 298 3186
Fragments arranged by size:
3186 1058 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 2582 4070 882
Size: 617 1041 42 1488 2219
Fragments arranged by size:
2219 1488 1041 617 42
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 2250 5401 2250
Size: 3151 2256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 2121 3214 3596
Size: 53 83 186 936 527 61 1093 382
Cuts at: 3596 4231 275
Size: 635 1451
Fragments arranged by size:
1451 1093 936 635 527 382 186 83
61 53
BsaWI w'CCGG_w
Cuts at: 2283 3840 4238 4512 4659 5070 2283
Size: 1557 398 274 147 411 2620
Fragments arranged by size:
2620 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 2270 2300 2411
Size: 30 165 30 738 30 99 30 111
Cuts at: 2411 2441 1178
Size: 30 4144
Fragments arranged by size:
4144 738 165 111 99 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 1186
Size: 1189 4218
BseYI C'CCAG_C
Cuts at: 2414 4610 5106 2414
Size: 2196 496 2715
Fragments arranged by size:
2715 2196 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2416 2471 1163
Size: 573 680 55 4099
Fragments arranged by size:
4099 680 573 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2226 2526 2857 3618 4646 1540
Size: 275 186 225 300 331 761 1028 2301
Fragments arranged by size:
2301 1028 761 331 300 275 225 186
BsiHKAI G_wGCw'C
Cuts at: 2034 3468 3553 4624 2034
Size: 1434 85 1071 2817
Fragments arranged by size:
2817 1434 1071 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2204 2440 2726 3468 3553 4624
Size: 399 244 170 236 286 742 85 1071
Cuts at: 4624 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 286 244 236 170
85
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2437 2762 844
Size: 1593 325 3489
Fragments arranged by size:
3489 1593 325
BsrBI CCG'CTC
Cuts at: 1394 1823 2086 3294 1394
Size: 429 263 1208 3507
Fragments arranged by size:
3507 1208 429 263
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2256 2283 2521 2561 4051 4238 1700
Size: 110 446 27 238 40 1490 187 2869
Fragments arranged by size:
2869 1490 446 238 187 110 40 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2319 2518 794
Size: 1525 199 3683
Fragments arranged by size:
3683 1525 199
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2484 2690 2737 3104 3489 3506 4958 5044
Size: 512 206 47 367 385 17 1452 86
Cuts at: 5044 5056 5402 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 512 385 367 346 206 86
47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2191 513
Size: 552 1126 3729
Fragments arranged by size:
3729 1126 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2144 2923 3717 3737 2144
Size: 779 794 20 3814
Fragments arranged by size:
3814 794 779 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DraIII CAC_nnn'GTG
Cuts at: 2653 2653
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2523 2854
Size: 54 1425 50 275 18 393 300 331
Cuts at: 2854 3743 5372 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 393 331 300 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2223 2523 2854 1537
Size: 275 411 300 331 4090
Fragments arranged by size:
4090 411 331 300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 2385 3337 1353
Size: 977 55 952 3423
Fragments arranged by size:
3423 977 952 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2297 2477 3470 4067 4854 5122
Size: 393 521 144 180 993 597 787 268
Cuts at: 5122 1239
Size: 1524
Fragments arranged by size:
1524 993 787 597 521 393 268 180
144
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2357
Size: 5407
EcoO109I rG'GnC_Cy
Cuts at: 2367 2367
Size: 5407
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2092 2124 2146 2556 4554 1678
Size: 414 32 22 410 1998 2531
Fragments arranged by size:
2531 1998 414 410 32 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2411 2443 2627 2659 4048
Size: 32 135 32 839 32 184 32 1389
Cuts at: 4048 4080 4122 4154 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1389 839 184 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 4705 1305
Size: 3216 184 2007
Fragments arranged by size:
3216 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3585
Fragments arranged by size:
3585 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 3365 3724 3883 518
Size: 648 882 1317 359 159 2042
Fragments arranged by size:
2042 1317 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2455 2528 2823 3500 4648 4893 1606
Size: 468 381 73 295 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 468 381 295 245 73
NaeI GCC'GGC
Cuts at: 2523 2523
Size: 5407
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 5407
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4855
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2521 2521
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2223 2854 2223
Size: 631 4776
NspI r_CATG'y
Cuts at: 1132 1229 2765 4310 1132
Size: 97 1536 1545 2229
Fragments arranged by size:
2229 1545 1536 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5407
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 1108
Size: 1089 4318
PfoI T'CCnGG_A
Cuts at: 2094 2094
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 2302 3476 3508 4989 5021 2270
Size: 32 1174 32 1481 32 2656
Fragments arranged by size:
2656 1481 1174 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2478 2977 2478
Size: 499 4908
PstI C_TGCA'G
Cuts at: 830 2073 2759 830
Size: 1243 686 3478
Fragments arranged by size:
3478 1243 686
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
PvuII CAG'CTG
Cuts at: 2455 2455
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SexAI A'CCwGG_T
Cuts at: 2601 2601
Size: 5407
SfcI C'TryA_G
Cuts at: 826 1045 2069 2755 3890 4571 4762 826
Size: 219 1024 686 1135 681 191 1471
Fragments arranged by size:
1471 1135 1024 686 681 219 191
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 5407
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2734 1872
Size: 862 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 3511 4412 4674 820
Size: 197 926 546 1022 901 262 1553
Fragments arranged by size:
1553 1022 926 901 546 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2370 2432 2846 513
Size: 552 1305 62 414 3074
Fragments arranged by size:
3074 1305 552 414 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2685 2692 3434 3451 3604 3789 4234
Size: 1255 1105 7 742 17 153 185 445
Cuts at: 4234 325
Size: 1498
Fragments arranged by size:
1498 1255 1105 742 445 185 153 17
7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 2493 3653
Size: 274 80 33 51 1443 231 285 1160
Cuts at: 3653 5365 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1160 285 274 231 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 2102 2423 1316
Size: 786 321 4300
Fragments arranged by size:
4300 786 321
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2732 1870
Size: 862 4545
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2061 3215 276
Size: 53 83 186 936 527 1154 2468
Fragments arranged by size:
2468 1154 936 527 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI XcmI