Restriction map for
pFN21AE3645
(Circular) (Six-base) MAPSORT of: pFN21AE3645.seq Check: 2003 from: 1 to: 4927
pFN21AE3645 4927 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2737 278
Size: 53 83 186 936 1201 2468
Fragments arranged by size:
2468 1201 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2270 1937
Size: 333 4594
Acc65I G'GTAC_C
Cuts at: 2248 2248
Size: 4927
AclI AA'CG_TT
Cuts at: 1563 3054 3427 1563
Size: 1491 373 3063
Fragments arranged by size:
3063 1491 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2990 4374 4642 1632
Size: 1358 1384 268 1917
Fragments arranged by size:
1917 1384 1358 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4730
AflIII A'CryG_T
Cuts at: 1203 3826 4803 1203
Size: 2623 977 1327
Fragments arranged by size:
2623 1327 977
AgeI A'CCGG_T
Cuts at: 3758 3758
Size: 4927
AhdI GACnn_n'nnGTC
Cuts at: 3656 3656
Size: 4927
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4927
AlwNI CAG_nnn'CTG
Cuts at: 2670 4242 2670
Size: 1572 3355
ApaLI G'TGCA_C
Cuts at: 2984 4140 2984
Size: 1156 3771
ApoI r'AATT_y
Cuts at: 1493 2236 2462 2614 4723 1493
Size: 743 226 152 2109 1697
Fragments arranged by size:
2109 1697 743 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3481 160
Size: 872 2449 1606
Fragments arranged by size:
2449 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4927
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 1870
Size: 73 309 4545
Fragments arranged by size:
4545 309 73
BamHI G'GATC_C
Cuts at: 2155 2257 2624 2155
Size: 102 367 4458
Fragments arranged by size:
4458 367 102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2248 3703 618
Size: 325 217 627 461 1455 1842
Fragments arranged by size:
1842 1455 627 461 325 217
BanII G_rGCy'C
Cuts at: 1391 2205 2246 1391
Size: 814 41 4072
Fragments arranged by size:
4072 814 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3118 3152 1903
Size: 34 50 34 1097 34 3678
Fragments arranged by size:
3678 1097 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2819 4029 2819
Size: 1210 3717
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4927
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3538 136
Size: 107 122 71 631 605 1866 1525
Fragments arranged by size:
1866 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4922 4922
Size: 4927
BlpI GC'TnA_GC
Cuts at: 2344 2344
Size: 4927
Bme1580I G_kGCm'C
Cuts at: 1790 2988 4144 1790
Size: 1198 1156 2573
Fragments arranged by size:
2573 1198 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2658 3616 442
Size: 416 53 83 399 24 1241 958 1753
Fragments arranged by size:
1753 1241 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3587 1239
Size: 2348 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 3052 3917 4215 2054
Size: 998 865 298 2766
Fragments arranged by size:
2766 998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3590 882
Size: 617 2091 2219
Fragments arranged by size:
2219 2091 617
BsaAI yAC'GTr
Cuts at: 493 1204 2718 493
Size: 711 1514 2702
Fragments arranged by size:
2702 1514 711
BsaBI GATnn'nnATC
Cuts at: 4921 4921
Size: 4927
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2734 3116 3751 275
Size: 53 83 186 936 1201 382 635 1451
Fragments arranged by size:
1451 1201 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3360 3758 4032 4179 4590 3360
Size: 398 274 147 411 3697
Fragments arranged by size:
3697 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2202 2232 1178
Size: 30 165 30 799 30 3873
Fragments arranged by size:
3873 799 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4927
BseYI C'CCAG_C
Cuts at: 4130 4626 4130
Size: 496 4431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4354
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2377 3138 4166 1540
Size: 275 186 376 761 1028 2301
Fragments arranged by size:
2301 1028 761 376 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 2988 3073 4144 2205
Size: 783 85 1071 2988
Fragments arranged by size:
2988 1071 783 85
BsmI GAATG_Cn'
Cuts at: 2100 2438 2531 2100
Size: 338 93 4496
Fragments arranged by size:
4496 338 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2246 2988 3073 4144 1391
Size: 399 415 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 85 41
BspEI T'CCGG_A
Cuts at: 4590 4590
Size: 4927
BspHI T'CATG_A
Cuts at: 2816 4546 2816
Size: 1730 3197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2282 844
Size: 1438 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2814 1394
Size: 429 991 3507
Fragments arranged by size:
3507 991 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3422 3596 65
Size: 1183 2174 174 1396
Fragments arranged by size:
2174 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3571 3758 1700
Size: 110 1761 187 2869
Fragments arranged by size:
2869 1761 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4927
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4927
BssSI C'ACGA_G
Cuts at: 2987 3999 2987
Size: 1012 3915
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4927
BstBI TT'CG_AA
Cuts at: 3731 3731
Size: 4927
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4927
BstYI r'GATC_y
Cuts at: 1972 2155 2257 2624 3009 3026 4478 4564 4576
Size: 183 102 367 385 17 1452 86 12
Cuts at: 4576 4922 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 183 102 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4375
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4188
Fragments arranged by size:
4188 683 56
BtsI GCAGTG_nn'
Cuts at: 2443 3237 3257 2443
Size: 794 20 4113
Fragments arranged by size:
4113 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4927
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4892
DraI TTT'AAA
Cuts at: 2231 2231
Size: 4927
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3934 809
Size: 507 2618 1802
Fragments arranged by size:
2618 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2374 3263 4892
Size: 54 1425 50 275 18 544 889 1629
Cuts at: 4892 8
Size: 43
Fragments arranged by size:
1629 1425 889 544 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2374 1537
Size: 275 562 4090
Fragments arranged by size:
4090 562 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2857 1353
Size: 1504 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3514 3888 4034 387
Size: 1064 138 1925 374 146 1280
Fragments arranged by size:
1925 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2203 2203
Size: 4927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2990 3587 4374 4642 1239
Size: 393 1358 597 787 268 1524
Fragments arranged by size:
1524 1358 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2026 2026
Size: 4927
EcoRI G'AATT_C
Cuts at: 2236 2614 2236
Size: 378 4549
FspI TGC'GCA
Cuts at: 3433 3433
Size: 4927
HaeII r_GCGC'y
Cuts at: 1678 4074 1678
Size: 2396 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3568 3600 3642 3674 1373
Size: 32 135 32 1996 32 42 32 2626
Fragments arranged by size:
2626 1996 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2062 2271 2517 669
Size: 988 281 124 209 246 3079
Fragments arranged by size:
3079 988 281 246 209 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4927
HpaI GTT'AAC
Cuts at: 2517 2517
Size: 4927
KpnI G_GTAC'C
Cuts at: 2252 2252
Size: 4927
MfeI C'AATT_G
Cuts at: 2526 2526
Size: 4927
MluI A'CGCG_T
Cuts at: 4803 4803
Size: 4927
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4041 4225 1305
Size: 2736 184 2007
Fragments arranged by size:
2736 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3105
Fragments arranged by size:
3105 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2885 3244 3403 518
Size: 648 1719 359 159 2042
Fragments arranged by size:
2042 1719 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2343 3020 4168 4413 1606
Size: 737 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4375
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4927
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4927
NotI GC'GGCC_GC
Cuts at: 2374 2374
Size: 4927
NspI r_CATG'y
Cuts at: 1132 1229 2285 3830 1132
Size: 97 1056 1545 2229
Fragments arranged by size:
2229 1545 1056 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4927
PciI A'CATG_T
Cuts at: 3826 3826
Size: 4927
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4927
PmeI GTTT'AAAC
Cuts at: 2231 2231
Size: 4927
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 4509 4541 2996
Size: 32 1481 32 3382
Fragments arranged by size:
3382 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 2026
Size: 4927
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4927
PsiI TTA'TAA
Cuts at: 2497 2497
Size: 4927
PstI C_TGCA'G
Cuts at: 830 2279 830
Size: 1449 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4927
SacI G_AGCT'C
Cuts at: 2205 2205
Size: 4927
SalI G'TCGA_C
Cuts at: 1936 2269 1936
Size: 333 4594
SbfI CC_TGCA'GG
Cuts at: 2279 2279
Size: 4927
ScaI AGT'ACT
Cuts at: 3175 4887 3175
Size: 1712 3215
SfcI C'TryA_G
Cuts at: 826 1045 2275 3410 4091 4282 826
Size: 219 1230 1135 681 191 1471
Fragments arranged by size:
1471 1230 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4927
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4927
SmaI CCC'GGG
Cuts at: 1872 2254 1872
Size: 382 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 3031 3932 4194 820
Size: 197 926 126 962 901 262 1553
Fragments arranged by size:
1553 962 926 901 262 197 126
SnaBI TAC'GTA
Cuts at: 493 2718 493
Size: 2225 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4927
SphI G_CATG'C
Cuts at: 1132 2285 1132
Size: 1153 3774
SspI AAT'ATT
Cuts at: 5 52 2020 2851 5
Size: 47 1968 831 2081
Fragments arranged by size:
2081 1968 831 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4927
StyI C'CwwG_G
Cuts at: 513 1065 2366 513
Size: 552 1301 3074
Fragments arranged by size:
3074 1301 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2954 2971 3124 3309 3754 325
Size: 1255 1374 17 153 185 445 1498
Fragments arranged by size:
1498 1374 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3173 4885 96
Size: 274 80 33 51 1443 1196 1712 138
Fragments arranged by size:
1712 1443 1196 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 4887 4919 2202
Size: 32 2653 32 2210
Fragments arranged by size:
2653 2210 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4927
XbaI T'CTAG_A
Cuts at: 2263 2263
Size: 4927
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4927
XmaI C'CCGG_G
Cuts at: 1870 2252 1870
Size: 382 4545
XmnI GAAnn'nnTTC
Cuts at: 3056 3056
Size: 4927
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2735 276
Size: 53 83 186 936 1201 2468
Fragments arranged by size:
2468 1201 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI