Restriction map for
pFN21AE3642
(Circular) (Six-base) MAPSORT of: pFN21AE3642.seq Check: 6184 from: 1 to: 5653
pFN21AE3642 5653 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2027 2547 2027
Size: 520 5133
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 2996 1937
Size: 1002 57 4594
Fragments arranged by size:
4594 1002 57
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2343 3716 5100 5368 1632
Size: 711 1373 1384 268 1917
Fragments arranged by size:
1917 1384 1373 711 268
AfeI AGC'GCT
Cuts at: 2900 2900
Size: 5653
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 2129 1166
Size: 963 4690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2207 2689 1184
Size: 1023 482 4148
Fragments arranged by size:
4148 1023 482
AlwNI CAG_nnn'CTG
Cuts at: 2350 3396 4968 2350
Size: 1046 1572 3035
Fragments arranged by size:
3035 1572 1046
ApaI G_GGCC'C
Cuts at: 2140 2140
Size: 5653
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2723 2962 3188 3340 5449 1493
Size: 1230 239 226 152 2109 1697
Fragments arranged by size:
2109 1697 1230 239 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2214 2983 3350 2214
Size: 769 367 4517
Fragments arranged by size:
4517 769 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 2974 4429 618
Size: 325 217 627 459 728 1455 1842
Fragments arranged by size:
1842 1455 728 627 459 325 217
BanII G_rGCy'C
Cuts at: 1391 2140 2972 1391
Size: 749 832 4072
Fragments arranged by size:
4072 832 749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 4264 136
Size: 107 122 71 631 605 367 2225 1525
Fragments arranged by size:
2225 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 2625 5648 2625
Size: 3023 2630
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2140 2374 3714 4870 1790
Size: 240 110 234 1340 1156 2573
Fragments arranged by size:
2573 1340 1156 240 234 110
BmgBI CAC'GTC
Cuts at: 1318 2893 1318
Size: 1575 4078
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2835 3384 4342
Size: 416 53 83 399 24 1418 549 958
Cuts at: 4342 442
Size: 1753
Fragments arranged by size:
1753 1418 958 549 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 1239
Size: 3074 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 3778 4643 4941 2147
Size: 1631 865 298 2859
Fragments arranged by size:
2859 1631 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2849 4316 882
Size: 617 1350 1467 2219
Fragments arranged by size:
2219 1467 1350 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 1178
Size: 30 165 30 1152 30 4246
Fragments arranged by size:
4246 1152 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 2453 2456 2762 2867 1186
Size: 899 368 3 306 105 3972
Fragments arranged by size:
3972 899 368 306 105 3
BseYI C'CCAG_C
Cuts at: 2029 4856 5352 2029
Size: 2827 496 2330
Fragments arranged by size:
2827 2330 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2590 3103 3864 4892 1540
Size: 275 186 589 513 761 1028 2301
Fragments arranged by size:
2301 1028 761 589 513 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 3714 3799 4870 2598
Size: 1116 85 1071 3381
Fragments arranged by size:
3381 1116 1071 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2140 2374 2598 2972 3714 3799
Size: 399 240 110 234 224 374 742 85
Cuts at: 3799 4870 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 240 234 224
110 85
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2027 2547 3008 844
Size: 1183 520 461 3489
Fragments arranged by size:
3489 1183 520 461
BsrBI CCG'CTC
Cuts at: 1394 1823 2446 3540 1394
Size: 429 623 1094 3507
Fragments arranged by size:
3507 1094 623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2295 2919 4297 4484 1700
Size: 110 485 624 1378 187 2869
Fragments arranged by size:
2869 1378 624 485 187 110
BsrGI T'GTAC_A
Cuts at: 96 2487 96
Size: 2391 3262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2473
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2214 2625 2983 3350 3735 3752 5204 5290
Size: 242 411 358 367 385 17 1452 86
Cuts at: 5290 5302 5648 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 411 385 367 358 346 242
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2035 2035
Size: 5653
BtgI C'CryG_G
Cuts at: 513 1065 2472 2697 513
Size: 552 1407 225 3469
Fragments arranged by size:
3469 1407 552 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2930 487
Size: 683 56 1704 3210
Fragments arranged by size:
3210 1704 683 56
BtsI GCAGTG_nn'
Cuts at: 3169 3963 3983 3169
Size: 794 20 4839
Fragments arranged by size:
4839 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2038 2073 529
Size: 35 1474 35 4109
Fragments arranged by size:
4109 1474 35 35
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2587 2647 2929
Size: 54 1425 50 275 18 757 60 282
Cuts at: 2929 3100 3989 5618 8
Size: 171 889 1629 43
Fragments arranged by size:
1629 1425 889 757 282 275 171 60
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2587 3100 1537
Size: 275 775 513 4090
Fragments arranged by size:
4090 775 513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 3583 1353
Size: 1250 980 3423
Fragments arranged by size:
3423 1250 980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2870 4240 4614 4760 387
Size: 1064 138 1281 1370 374 146 1280
Fragments arranged by size:
1370 1281 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 3716 4313 5100 5368 1239
Size: 393 711 1373 597 787 268 1524
Fragments arranged by size:
1524 1373 787 711 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2435 2342
Size: 93 5560
EcoO109I rG'GnC_Cy
Cuts at: 2137 2137
Size: 5653
EcoRI G'AATT_C
Cuts at: 2723 2962 3340 2723
Size: 239 378 5036
Fragments arranged by size:
5036 378 239
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 2902 4800 1678
Size: 1224 1898 2531
Fragments arranged by size:
2531 1898 1224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 4294 4326 4368
Size: 32 135 32 983 32 1707 32 42
Cuts at: 4368 4400 1373
Size: 32 2626
Fragments arranged by size:
2626 1707 983 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2784 4767 4951 1305
Size: 1479 1983 184 2007
Fragments arranged by size:
2007 1983 1479 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2649 10
Size: 54 1425 343 817 3014
Fragments arranged by size:
3014 1425 817 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2189 3611 3970 4129 518
Size: 648 963 60 1422 359 159 2042
Fragments arranged by size:
2042 1422 963 648 359 159 60
MspA1I CmG'CkG
Cuts at: 1606 3069 3746 4894 5139 1606
Size: 1463 677 1148 245 2120
Fragments arranged by size:
2120 1463 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NruI TCG'CGA
Cuts at: 2949 2949
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 1108
Size: 1242 4411
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspOMI G'GGCC_C
Cuts at: 2136 2136
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2379 3005 830
Size: 1549 626 3478
Fragments arranged by size:
3478 1549 626
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2603 2603
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 2379 3005 2379
Size: 626 5027
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2375 2902 3001 4136 4817 5008 826
Size: 219 1330 527 99 1135 681 191 1471
Fragments arranged by size:
1471 1330 1135 681 527 219 191 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 3757 4658 4920 820
Size: 197 926 219 1595 901 262 1553
Fragments arranged by size:
1595 1553 926 901 262 219 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2715 3092 513
Size: 552 1650 377 3074
Fragments arranged by size:
3074 1650 552 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 3680 3697 3850 4035 4480 325
Size: 1255 633 1467 17 153 185 445 1498
Fragments arranged by size:
1498 1467 1255 633 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2487 3899
Size: 274 80 33 51 1443 141 369 1412
Cuts at: 3899 5611 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1412 369 274 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 5613 5645 2745
Size: 32 2836 32 2753
Fragments arranged by size:
2836 2753 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2876 1316
Size: 1560 4093
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 2470
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI