Restriction map for
pFN21AE3641
(Circular) (Six-base) MAPSORT of: pFN21AE3641.seq Check: 1616 from: 1 to: 6214
pFN21AE3641 6214 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2536 2536
Size: 6214
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 4277 5661 5929 1632
Size: 401 2244 1384 268 1917
Fragments arranged by size:
2244 1917 1384 401 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 5113 6090 1203
Size: 3910 977 1327
Fragments arranged by size:
3910 1327 977
AgeI A'CCGG_T
Cuts at: 2039 5045 2039
Size: 3006 3208
AhdI GACnn_n'nnGTC
Cuts at: 2940 3253 4943 2940
Size: 313 1690 4211
Fragments arranged by size:
4211 1690 313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 2455 2487 2560 2641 2701 2722 2782 2905 2932
Size: 32 73 81 60 21 60 123 27
Cuts at: 2932 3123 3467 3957 5529 2455
Size: 191 344 490 1572 3140
Fragments arranged by size:
3140 1572 490 344 191 123 81 73
60 60 32 27 21
ApaI G_GGCC'C
Cuts at: 2083 2097 2131 3242 2083
Size: 14 34 1111 5055
Fragments arranged by size:
5055 1111 34 14
ApaLI G'TGCA_C
Cuts at: 2549 2830 4271 5427 2549
Size: 281 1441 1156 3336
Fragments arranged by size:
3336 1441 1156 281
ApoI r'AATT_y
Cuts at: 1493 2767 3152 3523 3749 3901 6010 1493
Size: 1274 385 371 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 385 371 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 3326 3539 1870
Size: 73 298 1085 213 4545
Fragments arranged by size:
4545 1085 298 213 73
BamHI G'GATC_C
Cuts at: 3505 3544 3911 3505
Size: 39 367 5808
Fragments arranged by size:
5808 367 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2254 2518 3306 3535 4990
Size: 325 217 627 467 264 788 229 1455
Cuts at: 4990 618
Size: 1842
Fragments arranged by size:
1842 1455 788 627 467 325 264 229
217
BanII G_rGCy'C
Cuts at: 1391 2083 2097 2131 2478 3242 3394 3533 1391
Size: 692 14 34 347 764 152 139 4072
Fragments arranged by size:
4072 764 692 347 152 139 34 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 928
Size: 971 317 4926
Fragments arranged by size:
4926 971 317
BbvCI CC'TCA_GC
Cuts at: 2391 2564 2391
Size: 173 6041
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2824 2858 4405 4439 1903
Size: 34 887 34 1547 34 3678
Fragments arranged by size:
3678 1547 887 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2333 2446 2501
Size: 107 122 71 631 605 661 113 55
Cuts at: 2501 4825 136
Size: 2324 1525
Fragments arranged by size:
2324 1525 661 631 605 122 113 107
71 55
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2097 2131 2191 2523 2553 2764 2834
Size: 293 14 34 60 332 30 211 70
Cuts at: 2834 3242 3382 4275 5431 1790
Size: 408 140 893 1156 2573
Fragments arranged by size:
2573 1156 893 408 332 293 211 140
70 60 34 30 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2998 3448 3945
Size: 416 53 83 399 24 1581 450 497
Cuts at: 3945 4903 442
Size: 958 1753
Fragments arranged by size:
1753 1581 958 497 450 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2883 2998 4874 1239
Size: 1644 115 1876 2579
Fragments arranged by size:
2579 1876 1644 115
Bpu10I CC'TnA_GC
Cuts at: 2391 2564 2894 2391
Size: 173 330 5711
Fragments arranged by size:
5711 330 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 4339 5204 5502 2639
Size: 1700 865 298 3351
Fragments arranged by size:
3351 1700 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3326 4877 882
Size: 617 1827 1551 2219
Fragments arranged by size:
2219 1827 1551 617
BsaAI yAC'GTr
Cuts at: 493 1204 3196 4005 493
Size: 711 1992 809 2702
Fragments arranged by size:
2702 1992 809 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3189 4021 4403 5038
Size: 53 83 186 936 1656 832 382 635
Cuts at: 5038 275
Size: 1451
Fragments arranged by size:
1656 1451 936 832 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2039 4647 5045 5319 5466 5877 2039
Size: 2608 398 274 147 411 2376
Fragments arranged by size:
2608 2376 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5989
Fragments arranged by size:
5989 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2486 2528 3344 1186
Size: 1000 300 42 816 4056
Fragments arranged by size:
4056 1000 816 300 42
BseYI C'CCAG_C
Cuts at: 2050 2103 2223 2809 2845 5417 5913 2050
Size: 53 120 586 36 2572 496 2351
Fragments arranged by size:
2572 2351 586 496 120 53 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3329 1163
Size: 573 1593 4048
Fragments arranged by size:
4048 1593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 2553 2834 4275 4360 5431 2478
Size: 75 281 1441 85 1071 3261
Fragments arranged by size:
3261 1441 1071 281 85 75
BsmI GAATG_Cn'
Cuts at: 2619 3179 3725 3818 2619
Size: 560 546 93 5015
Fragments arranged by size:
5015 560 546 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2097 2131 2191 2478 2523 2553
Size: 399 293 14 34 60 287 45 30
Cuts at: 2553 2764 2834 3242 3382 3394 3533 4275 4360
Size: 211 70 408 140 12 139 742 85
Cuts at: 4360 5431 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 293 287 211
140 139 85 70 60 45 34 30
14 12
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2536 2649 2913 3569 844
Size: 1692 113 264 656 3489
Fragments arranged by size:
3489 1692 656 264 113
BsrBI CCG'CTC
Cuts at: 1394 1823 2238 2413 4101 1394
Size: 429 415 175 1688 3507
Fragments arranged by size:
3507 1688 429 415 175
BsrDI GCAATG_nn'
Cuts at: 65 1248 4709 4883 65
Size: 1183 3461 174 1396
Fragments arranged by size:
3461 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2039 3309 3470 4858 5045 1700
Size: 110 229 1270 161 1388 187 2869
Fragments arranged by size:
2869 1388 1270 229 187 161 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 4274 5286 4274
Size: 1012 5202
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2701 2710 3467 794
Size: 1907 9 757 3541
Fragments arranged by size:
3541 1907 757 9
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 3025 1184
Size: 1841 4373
BstXI CCAn_nnnn'nTGG
Cuts at: 2063 2063
Size: 6214
BstYI r'GATC_y
Cuts at: 1972 2592 3505 3544 3911 4296 4313 5765 5851
Size: 620 913 39 367 385 17 1452 86
Cuts at: 5851 5863 6209 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 913 620 385 367 346 86
39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2676 2814 513
Size: 552 1611 138 3913
Fragments arranged by size:
3913 1611 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2643 3382 487
Size: 683 56 1417 739 3319
Fragments arranged by size:
3319 1417 739 683 56
BtsI GCAGTG_nn'
Cuts at: 3210 3465 3730 4524 4544 3210
Size: 255 265 794 20 4880
Fragments arranged by size:
4880 794 265 255 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 3025 3025
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2325 2583
Size: 54 1425 50 275 18 450 45 258
Cuts at: 2583 3661 4550 6179 8
Size: 1078 889 1629 43
Fragments arranged by size:
1629 1425 1078 889 450 275 258 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2463 3419 4144 1353
Size: 1110 956 725 3423
Fragments arranged by size:
3423 1110 956 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2408 4801 5175 5321 387
Size: 1064 138 819 2393 374 146 1280
Fragments arranged by size:
2393 1280 1064 819 374 146 138
EcoICRI GAG'CTC
Cuts at: 2476 2476
Size: 6214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2883 2998 4277 4874 5661 5929
Size: 393 401 850 115 1279 597 787 268
Cuts at: 5929 1239
Size: 1524
Fragments arranged by size:
1524 1279 850 787 597 401 393 268
115
EcoNI CCTnn'n_nnAGG
Cuts at: 2157 2168 2897 3416 2157
Size: 11 729 519 4955
Fragments arranged by size:
4955 729 519 11
EcoO109I rG'GnC_Cy
Cuts at: 2023 2079 2080 2087 2094 2557 2806 2869 2890
Size: 56 1 7 7 463 249 63 21
Cuts at: 2890 2902 3145 3238 3239 3283 3409 2023
Size: 12 243 93 1 44 126 4828
Fragments arranged by size:
4828 463 249 243 126 93 63 56
44 21 12 7 7 1 1
EcoRI G'AATT_C
Cuts at: 3523 3901 3523
Size: 378 5836
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 2430 5361 1678
Size: 752 2931 2531
Fragments arranged by size:
2931 2531 752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2926 2958 3111 3143 3239
Size: 32 135 32 1354 32 153 32 96
Cuts at: 3239 3271 3381 3413 4855 4887 4929 4961 1373
Size: 32 110 32 1442 32 42 32 2626
Fragments arranged by size:
2626 1442 1354 153 135 110 96 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3558 3804 669
Size: 988 281 1620 246 3079
Fragments arranged by size:
3079 1620 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 2538 3813 2538
Size: 1275 4939
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3210 5328 5512 1305
Size: 1905 2118 184 2007
Fragments arranged by size:
2118 2007 1905 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 10
Size: 54 1425 343 450 3942
Fragments arranged by size:
3942 1425 450 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 3293 4172 4531 4690 518
Size: 648 1923 204 879 359 159 2042
Fragments arranged by size:
2042 1923 879 648 359 204 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2103 2227 2395 2445 2531 2678 2779
Size: 444 53 124 168 50 86 147 101
Cuts at: 2779 3107 3630 4307 5455 5700 1606
Size: 328 523 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 523 444 328 245 168
147 124 101 86 53 50
NaeI GCC'GGC
Cuts at: 3472 3472
Size: 6214
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5662
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NgoMIV G'CCGG_C
Cuts at: 3470 3470
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 3572 5117 1132
Size: 97 2343 1545 2229
Fragments arranged by size:
2343 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2083 3235 1348
Size: 735 1152 4327
Fragments arranged by size:
4327 1152 735
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6214
PfoI T'CCnGG_A
Cuts at: 3417 3417
Size: 6214
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PmlI CAC'GTG
Cuts at: 3196 3196
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2806 2869 2902 3145 3283 3409 2806
Size: 63 33 243 138 126 5611
Fragments arranged by size:
5611 243 138 126 63 33
PshAI GACnn'nnGTC
Cuts at: 1613 2742 1613
Size: 1129 5085
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PspOMI G'GGCC_C
Cuts at: 2079 2093 2127 3238 2079
Size: 14 34 1111 5055
Fragments arranged by size:
5055 1111 34 14
PstI C_TGCA'G
Cuts at: 830 2367 2400 2706 3106 3135 3407 3566 830
Size: 1537 33 306 400 29 272 159 3478
Fragments arranged by size:
3478 1537 400 306 272 159 33 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2050 2227 2395 2779 3107 2050
Size: 177 168 384 328 5157
Fragments arranged by size:
5157 384 328 177 168
SacI G_AGCT'C
Cuts at: 2478 2478
Size: 6214
SacII CC_GC'GG
Cuts at: 2679 2679
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SanDI GG'GwC_CC
Cuts at: 2806 2869 2806
Size: 63 6151
SbfI CC_TGCA'GG
Cuts at: 2706 3407 3566 2706
Size: 701 159 5354
Fragments arranged by size:
5354 701 159
ScaI AGT'ACT
Cuts at: 2322 4462 6174 2322
Size: 2140 1712 2362
Fragments arranged by size:
2362 2140 1712
SexAI A'CCwGG_T
Cuts at: 3021 3021
Size: 6214
SfcI C'TryA_G
Cuts at: 826 1045 2363 2396 2702 3102 3131 3403 3562
Size: 219 1318 33 306 400 29 272 159
Cuts at: 3562 4697 5378 5569 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1318 1135 681 400 306 272 219
191 159 33 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 2501 2501
Size: 6214
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2241 2654 4318 5219 5481 820
Size: 197 926 298 413 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 413 298 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 2163 2708 1517
Size: 646 545 5023
Fragments arranged by size:
5023 646 545
StyI C'CwwG_G
Cuts at: 513 1065 2496 2508 3174 3653 513
Size: 552 1431 12 666 479 3074
Fragments arranged by size:
3074 1431 666 552 479 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2156 3248 3490 4241 4258 4411 4596
Size: 1255 576 1092 242 751 17 153 185
Cuts at: 4596 5041 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1092 751 576 445 242 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2320 4460 6172
Size: 274 80 33 51 1443 343 2140 1712
Cuts at: 6172 96
Size: 138
Fragments arranged by size:
2140 1712 1443 343 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2286 2318 6174 6206 2286
Size: 32 3856 32 2294
Fragments arranged by size:
3856 2294 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3125 1316
Size: 1809 4405
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2150 2150
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 2241 1943
Size: 298 5916
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 4343 4343
Size: 6214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SapI SfoI SrfI SwaI