Restriction map for
pFN21AE3640
(Circular) (Six-base) MAPSORT of: pFN21AE3640.seq Check: 8215 from: 1 to: 7171
pFN21AE3640 7171 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4981 278
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4514 1937
Size: 2577 4594
Acc65I G'GTAC_C
Cuts at: 4492 4492
Size: 7171
AclI AA'CG_TT
Cuts at: 1563 5298 5671 1563
Size: 3735 373 3063
Fragments arranged by size:
3735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2196 2208 3450 5234 6618 6886 1632
Size: 564 12 1242 1784 1384 268 1917
Fragments arranged by size:
1917 1784 1384 1242 564 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6974
AflIII A'CryG_T
Cuts at: 1203 2612 4399 6070 7047 1203
Size: 1409 1787 1671 977 1327
Fragments arranged by size:
1787 1671 1409 1327 977
AgeI A'CCGG_T
Cuts at: 6002 6002
Size: 7171
AhdI GACnn_n'nnGTC
Cuts at: 5900 5900
Size: 7171
AleI CACnn'nnGTG
Cuts at: 1166 4400 1166
Size: 3234 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4296 1184
Size: 3112 4059
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 2019
Size: 32 7139
AlwNI CAG_nnn'CTG
Cuts at: 2908 3845 4914 6486 2908
Size: 937 1069 1572 3593
Fragments arranged by size:
3593 1572 1069 937
ApaLI G'TGCA_C
Cuts at: 5228 6384 5228
Size: 1156 6015
ApoI r'AATT_y
Cuts at: 1493 2257 2563 3378 3667 3739 4480 4706 4858
Size: 764 306 815 289 72 741 226 152
Cuts at: 4858 6967 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 815 764 741 306 289 226
152 72
AseI AT'TA_AT
Cuts at: 160 1032 5725 160
Size: 872 4693 1606
Fragments arranged by size:
4693 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 4496 1870
Size: 73 2553 4545
Fragments arranged by size:
4545 2553 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4427 4460 4427
Size: 33 7138
BamHI G'GATC_C
Cuts at: 4501 4868 4501
Size: 367 6804
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3677 4492 5947 618
Size: 325 217 627 1890 815 1455 1842
Fragments arranged by size:
1890 1842 1455 815 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4490 1391
Size: 3099 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3373 3438 928
Size: 971 1474 65 4661
Fragments arranged by size:
4661 1474 971 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 5362 5396 1903
Size: 34 2000 34 1391 34 3678
Fragments arranged by size:
3678 2000 1391 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5063 6273 5063
Size: 1210 5961
BclI T'GATC_A
Cuts at: 1548 3099 3109 3175 1548
Size: 1551 10 66 5544
Fragments arranged by size:
5544 1551 66 10
BfrBI ATG'CAT
Cuts at: 3514 3514
Size: 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2244 5782 136
Size: 107 122 71 631 605 572 3538 1525
Fragments arranged by size:
3538 1525 631 605 572 122 107 71
BglII A'GATC_T
Cuts at: 2300 7166 2300
Size: 4866 2305
BlpI GC'TnA_GC
Cuts at: 4588 4588
Size: 7171
Bme1580I G_kGCm'C
Cuts at: 1790 5232 6388 1790
Size: 3442 1156 2573
Fragments arranged by size:
3442 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3158 4902 5860
Size: 416 53 83 399 24 1741 1744 958
Cuts at: 5860 442
Size: 1753
Fragments arranged by size:
1753 1744 1741 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2359 2745 3659 5831 1239
Size: 1120 386 914 2172 2579
Fragments arranged by size:
2579 2172 1120 914 386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 5296 6161 6459 2774
Size: 2522 865 298 3486
Fragments arranged by size:
3486 2522 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2804 5834 882
Size: 617 1305 3030 2219
Fragments arranged by size:
3030 2219 1305 617
BsaAI yAC'GTr
Cuts at: 493 1204 2356 4962 493
Size: 711 1152 2606 2702
Fragments arranged by size:
2702 2606 1152 711
BsaBI GATnn'nnATC
Cuts at: 3207 3234 3633 7165 3207
Size: 27 399 3532 3213
Fragments arranged by size:
3532 3213 399 27
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2294 4978 5360 5995
Size: 53 83 186 936 761 2684 382 635
Cuts at: 5995 275
Size: 1451
Fragments arranged by size:
2684 1451 936 761 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4150 5604 6002 6276 6423 6834 4150
Size: 1454 398 274 147 411 4487
Fragments arranged by size:
4487 1454 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3640 3670 3970 4000 1178
Size: 30 165 30 2237 30 300 30 4349
Fragments arranged by size:
4349 2237 300 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4059 1186
Size: 2873 4298
BseYI C'CCAG_C
Cuts at: 6374 6870 6374
Size: 496 6675
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2095 1163
Size: 573 359 6239
Fragments arranged by size:
6239 573 359
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4621 5382 6410 1540
Size: 275 186 2620 761 1028 2301
Fragments arranged by size:
2620 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5232 5317 6388 5232
Size: 85 1071 6015
Fragments arranged by size:
6015 1071 85
BsmI GAATG_Cn'
Cuts at: 3751 4682 4775 3751
Size: 931 93 6147
Fragments arranged by size:
6147 931 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4490 5232 5317 6388 1391
Size: 399 2700 742 85 1071 2174
Fragments arranged by size:
2700 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 4150 6834 4150
Size: 2684 4487
BspHI T'CATG_A
Cuts at: 3136 5060 6790 3136
Size: 1924 1730 3517
Fragments arranged by size:
3517 1924 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4526 844
Size: 3682 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5058 1394
Size: 429 3235 3507
Fragments arranged by size:
3507 3235 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3706 5666 5840 65
Size: 1183 2458 1960 174 1396
Fragments arranged by size:
2458 1960 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5815 6002 1700
Size: 110 4005 187 2869
Fragments arranged by size:
4005 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 5231 6243 5231
Size: 1012 6159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4411 794
Size: 3617 3554
BstBI TT'CG_AA
Cuts at: 5975 5975
Size: 7171
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7171
BstYI r'GATC_y
Cuts at: 1972 2300 3835 4501 4868 5253 5270 6722 6808
Size: 328 1535 666 367 385 17 1452 86
Cuts at: 6808 6820 7166 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1535 1452 666 385 367 346 328
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5680
Fragments arranged by size:
5680 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6432
Fragments arranged by size:
6432 683 56
BtsI GCAGTG_nn'
Cuts at: 2874 4278 4382 4687 5481 5501 2874
Size: 1404 104 305 794 20 4544
Fragments arranged by size:
4544 1404 794 305 104 20
ClaI AT'CG_AT
Cuts at: 1554 2043 1554
Size: 489 6682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2703 2738 529
Size: 35 2139 35 4962
Fragments arranged by size:
4962 2139 35 35
DraI TTT'AAA
Cuts at: 2878 3402 3672 4092 4475 2878
Size: 524 270 420 383 5574
Fragments arranged by size:
5574 524 420 383 270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6178 809
Size: 507 4862 1802
Fragments arranged by size:
4862 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 3550 4618
Size: 54 1425 50 275 18 406 1314 1068
Cuts at: 4618 5507 7136 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1314 1068 889 406 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4618 1537
Size: 275 2806 4090
Fragments arranged by size:
4090 2806 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3759 4089 5101 1353
Size: 2406 330 1012 3423
Fragments arranged by size:
3423 2406 1012 330
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5758 6132 6278 387
Size: 1064 138 4169 374 146 1280
Fragments arranged by size:
4169 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 2208 2359 2745 3450 3659 5234
Size: 393 564 12 151 386 705 209 1575
Cuts at: 5234 5831 6618 6886 1239
Size: 597 787 268 1524
Fragments arranged by size:
1575 1524 787 705 597 564 393 386
268 209 151 12
EcoO109I rG'GnC_Cy
Cuts at: 2765 2765
Size: 7171
EcoRI G'AATT_C
Cuts at: 2563 4480 4858 2563
Size: 1917 378 4876
Fragments arranged by size:
4876 1917 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2987 2987
Size: 7171
FspI TGC'GCA
Cuts at: 5677 5677
Size: 7171
HaeII r_GCGC'y
Cuts at: 1678 6318 1678
Size: 4640 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 5812 5844 5886
Size: 32 135 32 464 32 3744 32 42
Cuts at: 5886 5918 1373
Size: 32 2626
Fragments arranged by size:
3744 2626 464 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3067 3532 4132 4515 4761 669
Size: 988 281 1129 465 600 383 246 3079
Fragments arranged by size:
3079 1129 988 600 465 383 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7171
HpaI GTT'AAC
Cuts at: 4132 4761 4132
Size: 629 6542
KpnI G_GTAC'C
Cuts at: 4496 4496
Size: 7171
MfeI C'AATT_G
Cuts at: 2697 4770 2697
Size: 2073 5098
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2982 3510 3925 6285 6469 1305
Size: 1677 528 415 2360 184 2007
Fragments arranged by size:
2360 2007 1677 528 415 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2238 3552 10
Size: 54 1425 343 406 1314 3629
Fragments arranged by size:
3629 1425 1314 406 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3402 3762 4400 5129 5488 5647 518
Size: 648 2236 360 638 729 359 159 2042
Fragments arranged by size:
2236 2042 729 648 638 360 359 159
MspA1I CmG'CkG
Cuts at: 1606 4587 5264 6412 6657 1606
Size: 2981 677 1148 245 2120
Fragments arranged by size:
2981 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5680
Fragments arranged by size:
5680 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7171
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NsiI A_TGCA'T
Cuts at: 3516 3516
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 2276 2429 2616 4028 4403 4529 6074
Size: 97 1047 153 187 1412 375 126 1545
Cuts at: 6074 1132
Size: 2229
Fragments arranged by size:
2229 1545 1412 1047 375 187 153 126
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7171
PciI A'CATG_T
Cuts at: 2612 4399 6070 2612
Size: 1787 1671 3713
Fragments arranged by size:
3713 1787 1671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7171
PfoI T'CCnGG_A
Cuts at: 3802 3802
Size: 7171
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5240 5272 6753 6785 5240
Size: 32 1481 32 5626
Fragments arranged by size:
5626 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7171
PsiI TTA'TAA
Cuts at: 4741 4741
Size: 7171
PstI C_TGCA'G
Cuts at: 830 4523 830
Size: 3693 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SapI GCTCTTCn'nnn_
Cuts at: 3759 3759
Size: 7171
SbfI CC_TGCA'GG
Cuts at: 4523 4523
Size: 7171
ScaI AGT'ACT
Cuts at: 4428 5419 7131 4428
Size: 991 1712 4468
Fragments arranged by size:
4468 1712 991
SfcI C'TryA_G
Cuts at: 826 1045 4519 5654 6335 6526 826
Size: 219 3474 1135 681 191 1471
Fragments arranged by size:
3474 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 4498 1872
Size: 2626 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2753 5275 6176 6438 820
Size: 197 926 810 2522 901 262 1553
Fragments arranged by size:
2522 1553 926 901 810 262 197
SnaBI TAC'GTA
Cuts at: 493 2356 4962 493
Size: 1863 2606 2702
Fragments arranged by size:
2702 2606 1863
SpeI A'CTAG_T
Cuts at: 152 3092 152
Size: 2940 4231
SphI G_CATG'C
Cuts at: 1132 2276 4529 1132
Size: 1144 2253 3774
Fragments arranged by size:
3774 2253 1144
SspI AAT'ATT
Cuts at: 5 52 2470 3478 3524 5095 5
Size: 47 2418 1008 46 1571 2081
Fragments arranged by size:
2418 2081 1571 1008 47 46
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7171
StyI C'CwwG_G
Cuts at: 513 1065 2004 3129 4290 4610 513
Size: 552 939 1125 1161 320 3074
Fragments arranged by size:
3074 1161 1125 939 552 320
SwaI ATTT'AAAT
Cuts at: 3402 3672 3402
Size: 270 6901
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5198 5215 5368 5553 5998 325
Size: 1255 3618 17 153 185 445 1498
Fragments arranged by size:
3618 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3413 3854 4426
Size: 274 80 33 51 1443 1436 441 572
Cuts at: 4426 5417 7129 96
Size: 991 1712 138
Fragments arranged by size:
1712 1443 1436 991 572 441 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3798 3830 7131 7163 3798
Size: 32 3301 32 3806
Fragments arranged by size:
3806 3301 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7171
XbaI T'CTAG_A
Cuts at: 4507 4507
Size: 7171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2246 2246
Size: 7171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7171
XmaI C'CCGG_G
Cuts at: 1870 4496 1870
Size: 2626 4545
XmnI GAAnn'nnTTC
Cuts at: 5300 5300
Size: 7171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4979 276
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI