Restriction map for
pFN21AE3639
(Circular) (Six-base) MAPSORT of: pFN21AE3639.seq Check: 7788 from: 1 to: 5842
pFN21AE3639 5842 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2759 2759
Size: 5842
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 3163 3163
Size: 5842
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 3905 5289 5557 1632
Size: 870 1403 1384 268 1917
Fragments arranged by size:
1917 1403 1384 870 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 2531 4741 5718 1203
Size: 1328 2210 977 1327
Fragments arranged by size:
2210 1328 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5842
AlwNI CAG_nnn'CTG
Cuts at: 3585 5157 3585
Size: 1572 4270
ApaLI G'TGCA_C
Cuts at: 2949 3899 5055 2949
Size: 950 1156 3736
Fragments arranged by size:
3736 1156 950
ApoI r'AATT_y
Cuts at: 1493 2266 2309 2476 3151 3377 3529 5638 1493
Size: 773 43 167 675 226 152 2109 1697
Fragments arranged by size:
2109 1697 773 675 226 167 152 43
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 3167 1870
Size: 73 1224 4545
Fragments arranged by size:
4545 1224 73
BamHI G'GATC_C
Cuts at: 3115 3172 3539 3115
Size: 57 367 5418
Fragments arranged by size:
5418 367 57
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2194 2559 3062 3163 4618
Size: 325 217 627 407 365 503 101 1455
Cuts at: 4618 618
Size: 1842
Fragments arranged by size:
1842 1455 627 503 407 365 325 217
101
BanII G_rGCy'C
Cuts at: 1391 3161 1391
Size: 1770 4072
BbeI G_GCGC'C
Cuts at: 3066 3066
Size: 5842
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3013 928
Size: 971 1114 3757
Fragments arranged by size:
3757 1114 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BfrBI ATG'CAT
Cuts at: 2868 2868
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4453 136
Size: 107 122 71 631 605 2781 1525
Fragments arranged by size:
2781 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5837 5837
Size: 5842
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 2552 2953 3903 5059 1790
Size: 762 401 950 1156 2573
Fragments arranged by size:
2573 1156 950 762 401
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3573 4531 442
Size: 416 53 83 399 24 2156 958 1753
Fragments arranged by size:
2156 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2784 4502 1239
Size: 829 716 1718 2579
Fragments arranged by size:
2579 1718 829 716
Bpu10I CC'TnA_GC
Cuts at: 3071 3071
Size: 5842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3967 4832 5130 3967
Size: 865 298 4679
Fragments arranged by size:
4679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2904 4505 882
Size: 617 1405 1601 2219
Fragments arranged by size:
2219 1601 1405 617
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3063 3649 4031 4666
Size: 53 83 186 936 1530 586 382 635
Cuts at: 4666 275
Size: 1451
Fragments arranged by size:
1530 1451 936 635 586 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4275 4673 4947 5094 5505 4275
Size: 398 274 147 411 4612
Fragments arranged by size:
4612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 4894
Fragments arranged by size:
4894 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 2117 3119 1186
Size: 843 88 1002 3909
Fragments arranged by size:
3909 1002 843 88
BseYI C'CCAG_C
Cuts at: 2374 3066 5045 5541 2374
Size: 692 1979 496 2675
Fragments arranged by size:
2675 1979 692 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 4053 5081 1540
Size: 275 186 1291 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2953 3903 3988 5059 2953
Size: 950 85 1071 3736
Fragments arranged by size:
3736 1071 950 85
BsmI GAATG_Cn'
Cuts at: 2238 3353 3446 2238
Size: 1115 93 4634
Fragments arranged by size:
4634 1115 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2552 2953 3161 3903 3988 5059 1391
Size: 399 762 401 208 742 85 1071 2174
Fragments arranged by size:
2174 1071 762 742 401 399 208 85
BspEI T'CCGG_A
Cuts at: 5505 5505
Size: 5842
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2759 2969 3197 844
Size: 1915 210 228 3489
Fragments arranged by size:
3489 1915 228 210
BsrBI CCG'CTC
Cuts at: 1394 1823 3109 3729 1394
Size: 429 1286 620 3507
Fragments arranged by size:
3507 1286 620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4337 4511 65
Size: 1183 3089 174 1396
Fragments arranged by size:
3089 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4486 4673 1700
Size: 110 2676 187 2869
Fragments arranged by size:
2869 2676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 2900 1184
Size: 1716 4126
BstYI r'GATC_y
Cuts at: 1972 2350 3115 3172 3539 3924 3941 5393 5479
Size: 378 765 57 367 385 17 1452 86
Cuts at: 5479 5491 5837 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 765 385 378 367 346 86
57 17 12
Bsu36I CC'TnA_GG
Cuts at: 3050 3050
Size: 5842
BtgI C'CryG_G
Cuts at: 513 1065 2004 2617 513
Size: 552 939 613 3738
Fragments arranged by size:
3738 939 613 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5103
Fragments arranged by size:
5103 683 56
BtsI GCAGTG_nn'
Cuts at: 2072 2825 3010 3358 4152 4172 2072
Size: 753 185 348 794 20 3742
Fragments arranged by size:
3742 794 753 348 185 20
ClaI AT'CG_AT
Cuts at: 1554 2124 1554
Size: 570 5272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 2527 3146 2527
Size: 619 5223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3289 4178 5807
Size: 54 1425 50 275 18 1459 889 1629
Cuts at: 5807 8
Size: 43
Fragments arranged by size:
1629 1459 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3772 1353
Size: 2419 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 4429 4803 4949 387
Size: 1064 138 507 2333 374 146 1280
Fragments arranged by size:
2333 1280 1064 507 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2502 2784 3905 4502 5289 5557
Size: 393 436 434 282 1121 597 787 268
Cuts at: 5557 1239
Size: 1524
Fragments arranged by size:
1524 1121 787 597 436 434 393 282
268
EcoO109I rG'GnC_Cy
Cuts at: 2766 2766
Size: 5842
EcoRI G'AATT_C
Cuts at: 2309 2476 3151 3529 2309
Size: 167 675 378 4622
Fragments arranged by size:
4622 675 378 167
EcoRV GAT'ATC
Cuts at: 2167 2167
Size: 5842
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 2022 3066 4989 1678
Size: 344 1044 1923 2531
Fragments arranged by size:
2531 1923 1044 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 4483 4515 4557
Size: 32 135 32 541 32 2338 32 42
Cuts at: 4557 4589 1373
Size: 32 2626
Fragments arranged by size:
2626 2338 541 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2537 3186 3432 669
Size: 988 281 599 649 246 3079
Fragments arranged by size:
3079 988 649 599 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5842
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KasI G'GCGC_C
Cuts at: 3062 3062
Size: 5842
KpnI G_GTAC'C
Cuts at: 3167 3167
Size: 5842
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2378 4956 5140 1305
Size: 1073 2578 184 2007
Fragments arranged by size:
2578 2007 1073 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4020
Fragments arranged by size:
4020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4159 4318 518
Size: 648 2634 359 159 2042
Fragments arranged by size:
2634 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3258 3935 5083 5328 1606
Size: 1652 677 1148 245 2120
Fragments arranged by size:
2120 1652 1148 677 245
NarI GG'CG_CC
Cuts at: 3063 3063
Size: 5842
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4351
Fragments arranged by size:
4351 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NsiI A_TGCA'T
Cuts at: 2870 2870
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 2535 3047 3200 4745 1132
Size: 97 1306 512 153 1545 2229
Fragments arranged by size:
2229 1545 1306 512 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 2531 4741 2531
Size: 2210 3632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2426 1108
Size: 1318 4524
PfoI T'CCnGG_A
Cuts at: 2800 2800
Size: 5842
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2403 3911 3943 5424 5456 2371
Size: 32 1508 32 1481 32 2757
Fragments arranged by size:
2757 1508 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2766 2766
Size: 5842
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PstI C_TGCA'G
Cuts at: 830 2287 3194 830
Size: 1457 907 3478
Fragments arranged by size:
3478 1457 907
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 2145 4090 5802 2145
Size: 1945 1712 2185
Fragments arranged by size:
2185 1945 1712
SfcI C'TryA_G
Cuts at: 826 1045 2283 3190 4325 5006 5197 826
Size: 219 1238 907 1135 681 191 1471
Fragments arranged by size:
1471 1238 1135 907 681 219 191
SfoI GGC'GCC
Cuts at: 3064 3064
Size: 5842
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 3169 1872
Size: 1297 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3946 4847 5109 820
Size: 197 926 2003 901 262 1553
Fragments arranged by size:
2003 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2004 3281 513
Size: 552 939 1277 3074
Fragments arranged by size:
3074 1277 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3869 3886 4039 4224 4669 325
Size: 1255 2289 17 153 185 445 1498
Fragments arranged by size:
2289 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2143 4088 5800
Size: 274 80 33 51 1443 166 1945 1712
Cuts at: 5800 96
Size: 138
Fragments arranged by size:
1945 1712 1443 274 166 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2661 2693 5802 5834 2661
Size: 32 3109 32 2669
Fragments arranged by size:
3109 2669 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 3167 1870
Size: 1297 4545
XmnI GAAnn'nnTTC
Cuts at: 3971 3971
Size: 5842
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI
FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI