Restriction map for
pFN21AE3638
(Circular) (Six-base) MAPSORT of: pFN21AE3638.seq Check: 4531 from: 1 to: 5983
pFN21AE3638 5983 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2773 3176 2773
Size: 403 5580
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 2895 3304 2895
Size: 409 5574
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2493 2685 4046 5430 5698 1632
Size: 370 491 192 1361 1384 268 1917
Fragments arranged by size:
1917 1384 1361 491 370 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 3194 1166
Size: 2028 3955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 2497 2529 2138
Size: 32 327 32 5592
Fragments arranged by size:
5592 327 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3050 3241 3726 5298 3050
Size: 191 485 1572 3735
Fragments arranged by size:
3735 1572 485 191
ApaI G_GGCC'C
Cuts at: 2921 2921
Size: 5983
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 2186 2450 2609 3292 3518 3670 5779 1493
Size: 693 264 159 683 226 152 2109 1697
Fragments arranged by size:
2109 1697 693 683 264 226 159 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 1870
Size: 73 1365 4545
Fragments arranged by size:
4545 1365 73
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2647 2895 3098 3122 3304
Size: 325 217 627 860 248 203 24 182
Cuts at: 3304 4759 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 860 627 325 248 217 203
182 24
BanII G_rGCy'C
Cuts at: 1391 2012 2040 2067 2921 2946 3234 3302 1391
Size: 621 28 27 854 25 288 68 4072
Fragments arranged by size:
4072 854 621 288 68 28 27 25
BbeI G_GCGC'C
Cuts at: 3126 3126
Size: 5983
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 928
Size: 971 345 4667
Fragments arranged by size:
4667 971 345
BbvCI CC'TCA_GC
Cuts at: 3051 3051
Size: 5983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2021 4594 136
Size: 107 122 71 631 605 349 2573 1525
Fragments arranged by size:
2573 1525 631 605 349 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2678 2921 4044 5200 1790
Size: 301 587 243 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 587 301 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 2404 3714
Size: 416 53 83 399 24 774 213 1310
Cuts at: 3714 4672 442
Size: 958 1753
Fragments arranged by size:
1753 1310 958 774 416 399 213 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 3218 4643 1239
Size: 842 1137 1425 2579
Fragments arranged by size:
2579 1425 1137 842
Bpu10I CC'TnA_GC
Cuts at: 3051 3051
Size: 5983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 2912 2930 2971 4108 4973 5271 2651
Size: 261 18 41 1137 865 298 3363
Fragments arranged by size:
3363 1137 865 298 261 41 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 4646 882
Size: 617 657 2490 2219
Fragments arranged by size:
2490 2219 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 3123 3790 4172
Size: 53 83 186 936 519 1071 667 382
Cuts at: 4172 4807 275
Size: 635 1451
Fragments arranged by size:
1451 1071 936 667 635 519 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2975 3005 3053 3083 1178
Size: 30 165 30 1572 30 48 30 4078
Fragments arranged by size:
4078 1572 165 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2432 2720 3284 1186
Size: 1246 288 564 3885
Fragments arranged by size:
3885 1246 564 288
BseYI C'CCAG_C
Cuts at: 2011 2517 5186 5682 2011
Size: 506 2669 496 2312
Fragments arranged by size:
2669 2312 506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2870 1163
Size: 573 1134 4276
Fragments arranged by size:
4276 1134 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 2747 2928 2946 4044 4129 5200 2103
Size: 644 181 18 1098 85 1071 2886
Fragments arranged by size:
2886 1098 1071 644 181 85 18
BsmI GAATG_Cn'
Cuts at: 2590 3494 3587 2590
Size: 904 93 4986
Fragments arranged by size:
4986 904 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2040 2067 2091 2103 2678 2747
Size: 399 222 28 27 24 12 575 69
Cuts at: 2747 2921 2928 2946 3234 3302 4044 4129 5200
Size: 174 7 18 288 68 742 85 1071
Cuts at: 5200 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 575 399 288 222 174
85 69 68 28 27 24 18 12
7
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 2817 3872 5602 2817
Size: 1055 1730 3198
Fragments arranged by size:
3198 1730 1055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2773 2907 3176 3338 844
Size: 1929 134 269 162 3489
Fragments arranged by size:
3489 1929 269 162 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3022 3870 1394
Size: 429 1199 848 3507
Fragments arranged by size:
3507 1199 848 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 3215 2032
Size: 1183 4800
BstYI r'GATC_y
Cuts at: 1972 2724 3313 3680 4065 4082 5534 5620 5632
Size: 752 589 367 385 17 1452 86 12
Cuts at: 5632 5978 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 752 589 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2336 2388 2336
Size: 52 5931
BtgI C'CryG_G
Cuts at: 513 1065 2025 2281 2830 3192 513
Size: 552 960 256 549 362 3304
Fragments arranged by size:
3304 960 552 549 362 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 2806 2958 3499 4293 4313 2806
Size: 152 541 794 20 4476
Fragments arranged by size:
4476 794 541 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3430 4319 5948
Size: 54 1425 50 275 18 1600 889 1629
Cuts at: 5948 8
Size: 43
Fragments arranged by size:
1629 1600 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 3913 1353
Size: 1070 1490 3423
Fragments arranged by size:
3423 1490 1070
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 4944 5090 387
Size: 1064 138 2981 374 146 1280
Fragments arranged by size:
2981 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2944 2944
Size: 5983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2081 2493 2685 3218 4046 4643
Size: 393 370 79 412 192 533 828 597
Cuts at: 4643 5430 5698 1239
Size: 787 268 1524
Fragments arranged by size:
1524 828 787 597 533 412 393 370
268 192 79
EcoNI CCTnn'n_nnAGG
Cuts at: 3071 3071
Size: 5983
EcoO109I rG'GnC_Cy
Cuts at: 2918 3064 2918
Size: 146 5837
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2072 2072
Size: 5983
FspI TGC'GCA
Cuts at: 2183 3143 4489 2183
Size: 960 1346 3677
Fragments arranged by size:
3677 1346 960
FspAI rTGC'GCAy
Cuts at: 3143 3143
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 3126 5130 1678
Size: 1448 2004 2531
Fragments arranged by size:
2531 2004 1448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3053 3085 4624 4656 4698
Size: 32 135 32 1481 32 1539 32 42
Cuts at: 4698 4730 1373
Size: 32 2626
Fragments arranged by size:
2626 1539 1481 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KasI G'GCGC_C
Cuts at: 3122 3122
Size: 5983
KpnI G_GTAC'C
Cuts at: 2899 3308 2899
Size: 409 5574
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2937 5097 5281 1305
Size: 1632 2160 184 2007
Fragments arranged by size:
2160 2007 1632 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4161
Fragments arranged by size:
4161 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2691 2762 2783 2816 3194 3941
Size: 648 864 661 71 21 33 378 747
Cuts at: 3941 4300 4459 518
Size: 359 159 2042
Fragments arranged by size:
2042 864 747 661 648 378 359 159
71 33 21
MspA1I CmG'CkG
Cuts at: 1606 3083 3399 4076 5224 5469 1606
Size: 1477 316 677 1148 245 2120
Fragments arranged by size:
2120 1477 1148 677 316 245
NarI GG'CG_CC
Cuts at: 3123 3123
Size: 5983
NcoI C'CATG_G
Cuts at: 513 1065 2025 2281 2830 513
Size: 552 960 256 549 3666
Fragments arranged by size:
3666 960 552 549 256
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2782 3341 4886 1132
Size: 97 1553 559 1545 2229
Fragments arranged by size:
2229 1553 1545 559 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5983
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PfoI T'CCnGG_A
Cuts at: 3036 3036
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3064 3064
Size: 5983
PshAI GACnn'nnGTC
Cuts at: 1613 2968 1613
Size: 1355 4628
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PspOMI G'GGCC_C
Cuts at: 2917 2917
Size: 5983
PstI C_TGCA'G
Cuts at: 830 2755 3173 3335 830
Size: 1925 418 162 3478
Fragments arranged by size:
3478 1925 418 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
PvuII CAG'CTG
Cuts at: 3083 3083
Size: 5983
SacI G_AGCT'C
Cuts at: 2946 2946
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SapI GCTCTTCn'nnn_
Cuts at: 2423 2423
Size: 5983
SbfI CC_TGCA'GG
Cuts at: 3173 3335 3173
Size: 162 5821
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SexAI A'CCwGG_T
Cuts at: 2076 2076
Size: 5983
SfcI C'TryA_G
Cuts at: 826 1045 2490 2751 3132 3169 3331 4466 5147
Size: 219 1445 261 381 37 162 1135 681
Cuts at: 5147 5338 826
Size: 191 1471
Fragments arranged by size:
1471 1445 1135 681 381 261 219 191
162 37
SfoI GGC'GCC
Cuts at: 3124 3124
Size: 5983
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 2927 2945 2986 4087 4988
Size: 197 926 723 261 18 41 1101 901
Cuts at: 4988 5250 820
Size: 262 1553
Fragments arranged by size:
1553 1101 926 901 723 262 261 197
41 18
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 2782 3341 1132
Size: 1650 559 3774
Fragments arranged by size:
3774 1650 559
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 2025 2141 2281 2537 2830 3422 513
Size: 552 960 116 140 256 293 592 3074
Fragments arranged by size:
3074 960 592 552 293 256 140 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2386 4010 4027 4180 4365 4810 325
Size: 1255 806 1624 17 153 185 445 1498
Fragments arranged by size:
1624 1498 1255 806 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 2481 2604
Size: 274 80 33 51 1443 222 282 123
Cuts at: 2604 4229 5941 96
Size: 1625 1712 138
Fragments arranged by size:
1712 1625 1443 282 274 222 138 123
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5943 5975 5943
Size: 32 5951
Tth111I GACn'n_nGTC
Cuts at: 1316 3251 1316
Size: 1935 4048
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2798 2798
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 4112 4112
Size: 5983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI