Restriction map for
pFN21AE3635
(Circular) (Six-base) MAPSORT of: pFN21AE3635.seq Check: 4146 from: 1 to: 6091
pFN21AE3635 6091 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3028 3028
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3357 3901 278
Size: 53 83 186 936 1821 544 2468
Fragments arranged by size:
2468 1821 936 544 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2122 4154 5538 5806 1632
Size: 490 2032 1384 268 1917
Fragments arranged by size:
2032 1917 1384 490 268
AfeI AGC'GCT
Cuts at: 3362 3362
Size: 6091
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2123 4990 5967 1203
Size: 920 2867 977 1327
Fragments arranged by size:
2867 1327 977 920
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2075 1184
Size: 891 5200
AlwNI CAG_nnn'CTG
Cuts at: 2614 3241 3834 5406 2614
Size: 627 593 1572 3299
Fragments arranged by size:
3299 1572 627 593
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2066 2237 3400 3626 3778 5887 1493
Size: 573 171 1163 226 152 2109 1697
Fragments arranged by size:
2109 1697 1163 573 226 171 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2430 3045 3416 1870
Size: 73 487 615 371 4545
Fragments arranged by size:
4545 615 487 371 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 3412 4867 618
Size: 325 217 627 325 1300 1455 1842
Fragments arranged by size:
1842 1455 1300 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2148 2319 2501 2556 3121 3410 1391
Size: 696 61 171 182 55 565 289 4072
Fragments arranged by size:
4072 696 565 289 182 171 61 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2128 928
Size: 971 229 4891
Fragments arranged by size:
4891 971 229
BbvCI CC'TCA_GC
Cuts at: 2540 2540
Size: 6091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2273 2532 2634 2723 3508 2273
Size: 259 102 89 785 4856
Fragments arranged by size:
4856 785 259 102 89
Bme1580I G_kGCm'C
Cuts at: 1790 2115 2903 3348 4152 5308 1790
Size: 325 788 445 804 1156 2573
Fragments arranged by size:
2573 1156 804 788 445 325
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 2499 3119
Size: 416 53 83 399 24 759 323 620
Cuts at: 3119 3822 4780 442
Size: 703 958 1753
Fragments arranged by size:
1753 958 759 703 620 416 399 323
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2790 2853 2722
Size: 68 63 5960
Fragments arranged by size:
5960 68 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2303 2595 2910 4751 1239
Size: 971 93 292 315 1841 2579
Fragments arranged by size:
2579 1841 971 315 292 93
Bpu10I CC'TnA_GC
Cuts at: 2081 2250 2540 2081
Size: 169 290 5632
Fragments arranged by size:
5632 290 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5081 5379 4216
Size: 865 298 4928
Fragments arranged by size:
4928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3354 3898 4280 4915
Size: 53 83 186 936 1821 544 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1821 1451 936 635 544 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2283 3207 4524 4922 5196 5343 5754 2283
Size: 924 1317 398 274 147 411 2620
Fragments arranged by size:
2620 1317 924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2289 2319 2426 2456 1178
Size: 30 165 30 886 30 107 30 4813
Fragments arranged by size:
4813 886 165 107 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 2528 1186
Size: 1291 51 4749
Fragments arranged by size:
4749 1291 51
BseYI C'CCAG_C
Cuts at: 2410 2549 2894 3087 3286 5294 5790 2410
Size: 139 345 193 199 2008 496 2711
Fragments arranged by size:
2711 2008 496 345 199 193 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2529 3077 1163
Size: 573 793 548 4177
Fragments arranged by size:
4177 793 573 548
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3342 3541 4302 5330 1540
Size: 275 186 1341 199 761 1028 2301
Fragments arranged by size:
2301 1341 1028 761 275 199 186
BsiHKAI G_wGCw'C
Cuts at: 2015 2148 3297 4152 4237 5308 2015
Size: 133 1149 855 85 1071 2798
Fragments arranged by size:
2798 1149 1071 855 133 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2087 2115 2148 2319 2501 2556
Size: 399 225 72 28 33 171 182 55
Cuts at: 2556 2903 3121 3297 3348 3410 4152 4237 5308
Size: 347 218 176 51 62 742 85 1071
Cuts at: 5308 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 347 225 218 182
176 171 85 72 62 55 51 33
28
BspEI T'CCGG_A
Cuts at: 2283 5754 2283
Size: 3471 2620
BspHI T'CATG_A
Cuts at: 2556 3980 5710 2556
Size: 1424 1730 2937
Fragments arranged by size:
2937 1730 1424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3028 3446 844
Size: 2184 418 3489
Fragments arranged by size:
3489 2184 418
BsrBI CCG'CTC
Cuts at: 1394 1823 2277 3978 1394
Size: 429 454 1701 3507
Fragments arranged by size:
3507 1701 454 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2361 2985 4586 4760 65
Size: 1183 1113 624 1601 174 1396
Fragments arranged by size:
1601 1396 1183 1113 624 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 3296 4151 5163 3296
Size: 855 1012 4224
Fragments arranged by size:
4224 1012 855
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2200 2662 2797 794
Size: 1406 462 135 4088
Fragments arranged by size:
4088 1406 462 135
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 3040 3331 1184
Size: 1856 291 3944
Fragments arranged by size:
3944 1856 291
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2860 3028 2128
Size: 732 168 5191
Fragments arranged by size:
5191 732 168
BstYI r'GATC_y
Cuts at: 1972 2286 3174 3421 3788 4173 4190 5642 5728
Size: 314 888 247 367 385 17 1452 86
Cuts at: 5728 5740 6086 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 888 385 367 346 314 247
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3107 3107
Size: 6091
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2482 487
Size: 683 56 1256 4096
Fragments arranged by size:
4096 1256 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DraIII CAC_nnn'GTG
Cuts at: 3127 3127
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2862 3312 3339
Size: 54 1425 50 275 18 1032 450 27
Cuts at: 3339 3538 4427 6056 8
Size: 199 889 1629 43
Fragments arranged by size:
1629 1425 1032 889 450 275 199 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3339 3538 1537
Size: 275 1527 199 4090
Fragments arranged by size:
4090 1527 275 199
EarI CTCTTCn'nnn_
Cuts at: 1353 2555 2720 2822 2999 4021 1353
Size: 1202 165 102 177 1022 3423
Fragments arranged by size:
3423 1202 1022 177 165 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2336 4678 5052 5198 387
Size: 1064 138 747 2342 374 146 1280
Fragments arranged by size:
2342 1280 1064 747 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2146
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2210 2303 2595 2910 4154 4751
Size: 393 490 88 93 292 315 1244 597
Cuts at: 4751 5538 5806 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1244 787 597 490 393 315 292
268 93 88
EcoNI CCTnn'n_nnAGG
Cuts at: 2543 2543
Size: 6091
EcoO109I rG'GnC_Cy
Cuts at: 2077 2323 2077
Size: 246 5845
EcoRI G'AATT_C
Cuts at: 2066 2237 3400 3778 2066
Size: 171 1163 378 4379
Fragments arranged by size:
4379 1163 378 171
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 3364 5238 1678
Size: 1686 1874 2531
Fragments arranged by size:
2531 1874 1686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2426 2458 2995
Size: 32 135 32 563 32 259 32 537
Cuts at: 2995 3027 4732 4764 4806 4838 1373
Size: 32 1705 32 42 32 2626
Fragments arranged by size:
2626 1705 563 537 259 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2946 5205 5389 1305
Size: 1641 2259 184 2007
Fragments arranged by size:
2259 2007 1641 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2864 10
Size: 54 1425 343 1032 3237
Fragments arranged by size:
3237 1425 1032 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2799 2927 3029 3329 4049 4408 4567
Size: 648 1633 128 102 300 720 359 159
Cuts at: 4567 518
Size: 2042
Fragments arranged by size:
2042 1633 720 648 359 300 159 128
102
MspA1I CmG'CkG
Cuts at: 1606 2302 2410 2513 2600 3507 4184 5332 5577
Size: 696 108 103 87 907 677 1148 245
Cuts at: 5577 1606
Size: 2120
Fragments arranged by size:
2120 1148 907 696 677 245 108 103
87
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2127 2429 3449 4994 1132
Size: 97 898 302 1020 1545 2229
Fragments arranged by size:
2229 1545 1020 898 302 97
PasI CC'CwG_GG
Cuts at: 1348 2087 1348
Size: 739 5352
PciI A'CATG_T
Cuts at: 2123 4990 2123
Size: 2867 3224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2527 1108
Size: 1419 4672
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2403 1613
Size: 790 5301
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 6059
PstI C_TGCA'G
Cuts at: 830 2064 2976 3443 830
Size: 1234 912 467 3478
Fragments arranged by size:
3478 1234 912 467
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2302 2410 2513 2302
Size: 108 103 5880
Fragments arranged by size:
5880 108 103
SacI G_AGCT'C
Cuts at: 2148 2148
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 2566 4339 6051 2566
Size: 1773 1712 2606
Fragments arranged by size:
2606 1773 1712
SexAI A'CCwGG_T
Cuts at: 2691 2691
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 2060 2214 2972 3439 4574 5255 5446
Size: 219 1015 154 758 467 1135 681 191
Cuts at: 5446 826
Size: 1471
Fragments arranged by size:
1471 1135 1015 758 681 467 219 191
154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 2432 3047 3418 1872
Size: 560 615 371 4545
Fragments arranged by size:
4545 615 560 371
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4195 5096 5358 820
Size: 197 926 2252 901 262 1553
Fragments arranged by size:
2252 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 2429 3449 1132
Size: 1297 1020 3774
Fragments arranged by size:
3774 1297 1020
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 1517
Size: 870 5221
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 3530 513
Size: 552 939 150 1376 3074
Fragments arranged by size:
3074 1376 939 552 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 2158 2363 3138 4118 4135 4288
Size: 1255 535 43 205 775 980 17 153
Cuts at: 4288 4473 4918 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 980 775 535 445 205 185
153 43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2038 2261 2564
Size: 274 80 33 51 1443 61 223 303
Cuts at: 2564 4337 6049 96
Size: 1773 1712 138
Fragments arranged by size:
1773 1712 1443 303 274 223 138 80
61 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2289 2321 6051 6083 2289
Size: 32 3730 32 2297
Fragments arranged by size:
3730 2297 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3028 3322 3028
Size: 294 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 2430 3045 3416 1870
Size: 560 615 371 4545
Fragments arranged by size:
4545 615 560 371
XmnI GAAnn'nnTTC
Cuts at: 2237 4220 2237
Size: 1983 4108
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3355 3899 276
Size: 53 83 186 936 1821 544 2468
Fragments arranged by size:
2468 1821 936 544 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI