Restriction map for
pFN21AE3630
(Circular) (Six-base) MAPSORT of: pFN21AE3630.seq Check: 7238 from: 1 to: 6328
pFN21AE3630 6328 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2689 2689
Size: 6328
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2498 3671 1937
Size: 561 1173 4594
Fragments arranged by size:
4594 1173 561
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 4455 4828 1563
Size: 2892 373 3063
Fragments arranged by size:
3063 2892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 2880 3426 4391 5775 6043 1632
Size: 893 355 546 965 1384 268 1917
Fragments arranged by size:
1917 1384 965 893 546 355 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6131
AflIII A'CryG_T
Cuts at: 1203 5227 6204 1203
Size: 4024 977 1327
Fragments arranged by size:
4024 1327 977
AgeI A'CCGG_T
Cuts at: 5159 5159
Size: 6328
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6328
AlwNI CAG_nnn'CTG
Cuts at: 2663 2890 3223 3542 4071 5643 2663
Size: 227 333 319 529 1572 3348
Fragments arranged by size:
3348 1572 529 333 319 227
ApaI G_GGCC'C
Cuts at: 2690 2826 2690
Size: 136 6192
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 3011 3227 3637 3863 4015 6124 1493
Size: 1518 216 410 226 152 2109 1697
Fragments arranged by size:
2109 1697 1518 410 226 216 152
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 3071 3296 3653 1870
Size: 73 700 428 225 357 4545
Fragments arranged by size:
4545 700 428 357 225 73
BamHI G'GATC_C
Cuts at: 3658 4025 3658
Size: 367 5961
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2648 3067 3313 3587 3649
Size: 325 217 627 861 419 246 274 62
Cuts at: 3649 5104 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 861 627 419 325 274 246
217 62
BanII G_rGCy'C
Cuts at: 1391 2528 2690 2826 3647 1391
Size: 1137 162 136 821 4072
Fragments arranged by size:
4072 1137 821 162 136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2868 3473 3579 928
Size: 971 969 605 106 3677
Fragments arranged by size:
3677 971 969 605 106
BbvCI CC'TCA_GC
Cuts at: 3252 3252
Size: 6328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3057 3091 3376 3410 4519 4553 1903
Size: 34 1120 34 285 34 1109 34 3678
Fragments arranged by size:
3678 1120 1109 285 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 2892 3270 1548
Size: 1344 378 4606
Fragments arranged by size:
4606 1344 378
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2431 3065 4939
Size: 107 122 71 631 605 759 634 1874
Cuts at: 4939 136
Size: 1525
Fragments arranged by size:
1874 1525 759 634 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3096 6323 3096
Size: 3227 3101
BlpI GC'TnA_GC
Cuts at: 3745 3745
Size: 6328
Bme1580I G_kGCm'C
Cuts at: 1790 2690 2826 2841 2979 3053 3170 3590 4389
Size: 900 136 15 138 74 117 420 799
Cuts at: 4389 5545 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 900 799 420 138 136 117
74 15
BmgBI CAC'GTC
Cuts at: 1318 2455 2630 1318
Size: 1137 175 5016
Fragments arranged by size:
5016 1137 175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4059 5017 442
Size: 416 53 83 399 24 2642 958 1753
Fragments arranged by size:
2642 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3097 3544 3097
Size: 447 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2685 3566 4988 1239
Size: 1007 439 881 1422 2579
Fragments arranged by size:
2579 1422 1007 881 439
Bpu10I CC'TnA_GC
Cuts at: 3252 3252
Size: 6328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2152 2708 4453 5318 5616 2152
Size: 556 1745 865 298 2864
Fragments arranged by size:
2864 1745 865 556 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3080 4991 882
Size: 617 1581 1911 2219
Fragments arranged by size:
2219 1911 1581 617
BsaAI yAC'GTr
Cuts at: 493 1204 3479 4119 493
Size: 711 2275 640 2702
Fragments arranged by size:
2702 2275 711 640
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4135 4517 5152 275
Size: 53 83 186 936 2602 382 635 1451
Fragments arranged by size:
2602 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2288 4761 5159 5433 5580 5991 2288
Size: 2473 398 274 147 411 2625
Fragments arranged by size:
2625 2473 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 1178
Size: 30 165 30 1038 30 5035
Fragments arranged by size:
5035 1038 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2248 3023 3425 3626 1186
Size: 1062 775 402 201 3888
Fragments arranged by size:
3888 1062 775 402 201
BseYI C'CCAG_C
Cuts at: 2030 2062 3440 5531 6027 2030
Size: 32 1378 2091 496 2331
Fragments arranged by size:
2331 2091 1378 496 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 3314 3370 1163
Size: 573 768 810 56 4121
Fragments arranged by size:
4121 810 768 573 56
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2293 2435 3778 4539 5567 1540
Size: 275 186 292 142 1343 761 1028 2301
Fragments arranged by size:
2301 1343 1028 761 292 275 186 142
BsiHKAI G_wGCw'C
Cuts at: 3624 4389 4474 5545 3624
Size: 765 85 1071 4407
Fragments arranged by size:
4407 1071 765 85
BsmI GAATG_Cn'
Cuts at: 3839 3932 3839
Size: 93 6235
BsmBI CGTCTCn'nnnn_
Cuts at: 2461 2461
Size: 6328
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2528 2690 2826 2841 2979 3053 3170
Size: 399 738 162 136 15 138 74 117
Cuts at: 3170 3590 3624 3647 4389 4474 5545 1391
Size: 420 34 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 738 420 399 162 138
136 117 85 74 34 23 15
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 4217 5947 4217
Size: 1730 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2689 3683 844
Size: 1845 994 3489
Fragments arranged by size:
3489 1845 994
BsrBI CCG'CTC
Cuts at: 1394 1823 2314 3184 4215 1394
Size: 429 491 870 1031 3507
Fragments arranged by size:
3507 1031 870 491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2586 3013 4823 4997 65
Size: 1183 1338 427 1810 174 1396
Fragments arranged by size:
1810 1396 1338 1183 427 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 2336 2618 3513 4972 5159 1700
Size: 110 407 119 282 895 1459 187 2869
Fragments arranged by size:
2869 1459 895 407 282 187 119 110
BsrGI T'GTAC_A
Cuts at: 96 2793 96
Size: 2697 3631
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 3343 4388 5400 3343
Size: 1045 1012 4271
Fragments arranged by size:
4271 1045 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6328
BstBI TT'CG_AA
Cuts at: 2805 5132 2805
Size: 2327 4001
BstEII G'GTnAC_C
Cuts at: 1184 2167 1184
Size: 983 5345
BstXI CCAn_nnnn'nTGG
Cuts at: 2959 3046 2959
Size: 87 6241
BstYI r'GATC_y
Cuts at: 1972 2887 3096 3658 4025 4410 4427 5879 5965
Size: 915 209 562 367 385 17 1452 86
Cuts at: 5965 5977 6323 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 915 562 385 367 346 209
86 17 12
BstZ17I GTA'TAC
Cuts at: 2499 2499
Size: 6328
BtgI C'CryG_G
Cuts at: 513 1065 3045 3129 3603 513
Size: 552 1980 84 474 3238
Fragments arranged by size:
3238 1980 552 474 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 3028 3844 4638 4658 3028
Size: 816 794 20 4698
Fragments arranged by size:
4698 816 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6293
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DraIII CAC_nnn'GTG
Cuts at: 2175 2175
Size: 6328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2443 5335 809
Size: 507 1127 2892 1802
Fragments arranged by size:
2892 1802 1127 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 2432 2654
Size: 54 1425 50 275 18 406 196 222
Cuts at: 2654 3160 3775 4664 6293 8
Size: 506 615 889 1629 43
Fragments arranged by size:
1629 1425 889 615 506 406 275 222
196 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2432 3775 1537
Size: 275 620 1343 4090
Fragments arranged by size:
4090 1343 620 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3402 4258 1353
Size: 2049 856 3423
Fragments arranged by size:
3423 2049 856
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3371 4915 5289 5435 387
Size: 1064 138 1782 1544 374 146 1280
Fragments arranged by size:
1782 1544 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2525 2685 2880 3426 3566 4391
Size: 393 614 279 160 195 546 140 825
Cuts at: 4391 4988 5775 6043 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 825 787 614 597 546 393 279
268 195 160 140
EcoO109I rG'GnC_Cy
Cuts at: 2110 2119 2245 2265 2686 2687 2764 2822 3220
Size: 9 126 20 421 1 77 58 398
Cuts at: 3220 3265 2110
Size: 45 5173
Fragments arranged by size:
5173 421 398 126 77 58 45 20
9 1
EcoRI G'AATT_C
Cuts at: 3011 3637 4015 3011
Size: 626 378 5324
Fragments arranged by size:
5324 626 378
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 3542 5475 1678
Size: 1864 1933 2531
Fragments arranged by size:
2531 1933 1864
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2352 2384 2658 2690 3199
Size: 32 135 32 780 32 274 32 509
Cuts at: 3199 3231 4969 5001 5043 5075 1373
Size: 32 1738 32 42 32 2626
Fragments arranged by size:
2626 1738 780 509 274 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2815 3672 3918 669
Size: 988 281 877 857 246 3079
Fragments arranged by size:
3079 988 877 857 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6328
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2944 2976 3063 3351 5442 5626 1305
Size: 1639 32 87 288 2091 184 2007
Fragments arranged by size:
2091 2007 1639 288 184 87 32
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2238 3162 10
Size: 54 1425 343 406 924 3176
Fragments arranged by size:
3176 1425 924 406 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 2742 3134 3278 4286 4645 4804
Size: 648 1302 274 392 144 1008 359 159
Cuts at: 4804 518
Size: 2042
Fragments arranged by size:
2042 1302 1008 648 392 359 274 159
144
MspA1I CmG'CkG
Cuts at: 1606 2488 3744 4421 5569 5814 1606
Size: 882 1256 677 1148 245 2120
Fragments arranged by size:
2120 1256 1148 882 677 245
NaeI GCC'GGC
Cuts at: 2620 3515 2620
Size: 895 5433
NcoI C'CATG_G
Cuts at: 513 1065 3129 513
Size: 552 2064 3712
Fragments arranged by size:
3712 2064 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NgoMIV G'CCGG_C
Cuts at: 2618 3513 2618
Size: 895 5433
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 3686 5231 1132
Size: 97 2457 1545 2229
Fragments arranged by size:
2457 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2320 2682 3336 1348
Size: 972 362 654 4340
Fragments arranged by size:
4340 972 654 362
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2663 1108
Size: 1555 4773
PfoI T'CCnGG_A
Cuts at: 2662 2766 2883 2662
Size: 104 117 6107
Fragments arranged by size:
6107 117 104
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 5910 5942 4397
Size: 32 1481 32 4783
Fragments arranged by size:
4783 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2265 2764 3265 2245
Size: 20 499 501 5308
Fragments arranged by size:
5308 501 499 20
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PspOMI G'GGCC_C
Cuts at: 2686 2822 2686
Size: 136 6192
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 6296
PstI C_TGCA'G
Cuts at: 830 3680 830
Size: 2850 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
PvuII CAG'CTG
Cuts at: 2488 2488
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SanDI GG'GwC_CC
Cuts at: 2265 2265
Size: 6328
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 2909 4576 6288 2909
Size: 1667 1712 2949
Fragments arranged by size:
2949 1712 1667
SfcI C'TryA_G
Cuts at: 826 1045 3676 4811 5492 5683 826
Size: 219 2631 1135 681 191 1471
Fragments arranged by size:
2631 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 3655 1872
Size: 1783 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2131 2723 4432 5333 5595 820
Size: 197 926 188 592 1709 901 262 1553
Fragments arranged by size:
1709 1553 926 901 592 262 197 188
SnaBI TAC'GTA
Cuts at: 493 3479 4119 493
Size: 2986 640 2702
Fragments arranged by size:
2986 2702 640
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 3686 1132
Size: 2554 3774
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 3113 1517
Size: 1596 4732
StyI C'CwwG_G
Cuts at: 513 1065 2818 3114 3129 3767 513
Size: 552 1753 296 15 638 3074
Fragments arranged by size:
3074 1753 638 552 296 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3122 4355 4372 4525 4710 5155 325
Size: 1255 1542 1233 17 153 185 445 1498
Fragments arranged by size:
1542 1498 1255 1233 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2793 2907 4574
Size: 274 80 33 51 1443 816 114 1667
Cuts at: 4574 6286 96
Size: 1712 138
Fragments arranged by size:
1712 1667 1443 816 274 138 114 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2441 2473 6288 6320 2441
Size: 32 3815 32 2449
Fragments arranged by size:
3815 2449 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6328
XmaI C'CCGG_G
Cuts at: 1870 3653 1870
Size: 1783 4545
XmnI GAAnn'nnTTC
Cuts at: 3500 4457 3500
Size: 957 5371
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI