Restriction map for
pFN21AE3625
(Circular) (Six-base) MAPSORT of: pFN21AE3625.seq Check: 102 from: 1 to: 6067
pFN21AE3625 6067 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4130 5514 5782 1632
Size: 2498 1384 268 1917
Fragments arranged by size:
2498 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2884 820
Size: 197 1867 4003
Fragments arranged by size:
4003 1867 197
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 2568 1166
Size: 1402 4665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 3810 5382 3810
Size: 1572 4495
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 2314 3376 3602 3754 5863 1493
Size: 821 1062 226 152 2109 1697
Fragments arranged by size:
2109 1697 1062 821 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2435 4621 160
Size: 872 1403 2186 1606
Fragments arranged by size:
2186 1606 1403 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 3392 1870
Size: 73 1449 4545
Fragments arranged by size:
4545 1449 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3388 4843 618
Size: 325 217 627 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2196 3386 1391
Size: 805 1190 4072
Fragments arranged by size:
4072 1190 805
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5096
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 2679 2805 3291 4128 5284 1790
Size: 889 126 486 837 1156 2573
Fragments arranged by size:
2573 1156 889 837 486 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2423 2889 4727 1239
Size: 971 213 466 1838 2579
Fragments arranged by size:
2579 1838 971 466 213
Bpu10I CC'TnA_GC
Cuts at: 2126 2126
Size: 6067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 3226 4192 5057 5355 2144
Size: 1082 966 865 298 2856
Fragments arranged by size:
2856 1082 966 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3047 4730 882
Size: 617 1548 1683 2219
Fragments arranged by size:
2219 1683 1548 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3874 4256 4891 275
Size: 53 83 186 936 2341 382 635 1451
Fragments arranged by size:
2341 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2648 4500 4898 5172 5319 5730 2648
Size: 1852 398 274 147 411 2985
Fragments arranged by size:
2985 1852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5842
Fragments arranged by size:
5842 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 1186
Size: 961 5106
BseYI C'CCAG_C
Cuts at: 2634 2661 2774 5270 5766 2634
Size: 27 113 2496 496 2935
Fragments arranged by size:
2935 2496 496 113 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2592 2910 1163
Size: 573 856 318 4320
Fragments arranged by size:
4320 856 573 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2534 3363 4128 4213 5284 2196
Size: 338 829 765 85 1071 2979
Fragments arranged by size:
2979 1071 829 765 338 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
BsmBI CGTCTCn'nnnn_
Cuts at: 2257 2257
Size: 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2534 2679 2805 3291 3363 3386
Size: 399 406 338 145 126 486 72 23
Cuts at: 3386 4128 4213 5284 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 406 399 338 145
126 85 72 23
BspEI T'CCGG_A
Cuts at: 2648 5730 2648
Size: 3082 2985
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3422 844
Size: 2578 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2733 3954 1394
Size: 429 910 1221 3507
Fragments arranged by size:
3507 1221 910 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2285 2489 3165 96
Size: 2189 204 676 2998
Fragments arranged by size:
2998 2189 676 204
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 2815 1184
Size: 1631 4436
BstYI r'GATC_y
Cuts at: 1972 3397 3764 4149 4166 5618 5704 5716 6062
Size: 1425 367 385 17 1452 86 12 346
Cuts at: 6062 1972
Size: 1977
Fragments arranged by size:
1977 1452 1425 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4576
Fragments arranged by size:
4576 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 2546 3583 4377 4397 2546
Size: 1037 794 20 4216
Fragments arranged by size:
4216 1037 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DraIII CAC_nnn'GTG
Cuts at: 3346 3346
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2915 3514 4403
Size: 54 1425 50 275 18 1085 599 889
Cuts at: 4403 6032 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1085 889 599 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3997 1353
Size: 2644 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 2194
Size: 6067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2423 2889 4130 4727 5514 5782
Size: 393 578 213 466 1241 597 787 268
Cuts at: 5782 1239
Size: 1524
Fragments arranged by size:
1524 1241 787 597 578 466 393 268
213
EcoO109I rG'GnC_Cy
Cuts at: 2658 2658
Size: 6067
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
EcoRV GAT'ATC
Cuts at: 2722 3133 2722
Size: 411 5656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2498 2872 2185
Size: 313 374 5380
Fragments arranged by size:
5380 374 313
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 5214 1678
Size: 3536 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 2862 2894 4708
Size: 32 135 32 920 32 338 32 1814
Cuts at: 4708 4740 4782 4814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1814 920 338 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2597 3244 748
Size: 1849 647 3571
Fragments arranged by size:
3571 1849 647
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 2919 3666 2919
Size: 747 5320
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5181 5365 1305
Size: 3876 184 2007
Fragments arranged by size:
3876 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2917 10
Size: 54 1425 343 1085 3160
Fragments arranged by size:
3160 1425 1085 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2568 3006 3320 4025 4384 4543 518
Size: 648 1402 438 314 705 359 159 2042
Fragments arranged by size:
2042 1402 705 648 438 359 314 159
MspA1I CmG'CkG
Cuts at: 1606 2576 3483 4160 5308 5553 1606
Size: 970 907 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 907 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4576
Fragments arranged by size:
4576 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 2244 2310 2784 3325 3425 4970 1132
Size: 97 1015 66 474 541 100 1545 2229
Fragments arranged by size:
2229 1545 1015 541 474 100 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PfoI T'CCnGG_A
Cuts at: 2866 2866
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2658 2658
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PstI C_TGCA'G
Cuts at: 830 2743 2991 3419 830
Size: 1913 248 428 3478
Fragments arranged by size:
3478 1913 428 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
SacI G_AGCT'C
Cuts at: 2196 2196
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SexAI A'CCwGG_T
Cuts at: 3342 3342
Size: 6067
SfcI C'TryA_G
Cuts at: 826 1045 2739 2987 3025 3415 4550 5231 5422
Size: 219 1694 248 38 390 1135 681 191
Cuts at: 5422 826
Size: 1471
Fragments arranged by size:
1694 1471 1135 681 390 248 219 191
38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 2884 3241 4171 5072 5334
Size: 197 926 216 725 357 930 901 262
Cuts at: 5334 820
Size: 1553
Fragments arranged by size:
1553 930 926 901 725 357 262 216
197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3325 3425 1132
Size: 2193 100 3774
Fragments arranged by size:
3774 2193 100
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2004 2820 3506 513
Size: 552 939 816 686 3074
Fragments arranged by size:
3074 939 816 686 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4094 4111 4264 4449 4894 325
Size: 1255 2514 17 153 185 445 1498
Fragments arranged by size:
2514 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2285 2489 3165
Size: 274 80 33 51 1443 308 204 676
Cuts at: 3165 3219 4313 6025 96
Size: 54 1094 1712 138
Fragments arranged by size:
1712 1443 1094 676 308 274 204 138
80 54 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 2956 1316
Size: 1640 4427
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI