Restriction map for
pFN21AE3624
(Circular) (Six-base) MAPSORT of: pFN21AE3624.seq Check: 9051 from: 1 to: 6520
pFN21AE3624 6520 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2592 3863 1937
Size: 655 1271 4594
Fragments arranged by size:
4594 1271 655
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 3530 4583 5967 6235 1632
Size: 416 1482 1053 1384 268 1917
Fragments arranged by size:
1917 1482 1384 1053 416 268
AfeI AGC'GCT
Cuts at: 2033 2033
Size: 6520
AflII C'TTAA_G
Cuts at: 820 1017 3586 820
Size: 197 2569 3754
Fragments arranged by size:
3754 2569 197
AflIII A'CryG_T
Cuts at: 1203 2826 3275 5419 6396 1203
Size: 1623 449 2144 977 1327
Fragments arranged by size:
2144 1623 1327 977 449
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 5249 5249
Size: 6520
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2531 1184
Size: 1347 5173
AlwNI CAG_nnn'CTG
Cuts at: 2422 2800 2857 4263 5835 2422
Size: 378 57 1406 1572 3107
Fragments arranged by size:
3107 1572 1406 378 57
ApaI G_GGCC'C
Cuts at: 2745 2745
Size: 6520
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 2779 3593 3829 4055 4207 6316 1493
Size: 1286 814 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 1286 814 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3368 5074 160
Size: 872 2336 1706 1606
Fragments arranged by size:
2336 1706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 2445 2761 2868 3845 1870
Size: 73 349 153 316 107 977 4545
Fragments arranged by size:
4545 977 349 316 153 107 73
BamHI G'GATC_C
Cuts at: 3531 3850 4217 3531
Size: 319 367 5834
Fragments arranged by size:
5834 367 319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2635 3841 5296 618
Size: 325 217 627 300 548 1206 1455 1842
Fragments arranged by size:
1842 1455 1206 627 548 325 300 217
BanII G_rGCy'C
Cuts at: 1391 2541 2559 2745 3011 3111 3269 3839 1391
Size: 1150 18 186 266 100 158 570 4072
Fragments arranged by size:
4072 1150 570 266 186 158 100 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2801 3231 3298 928
Size: 971 902 430 67 4150
Fragments arranged by size:
4150 971 902 430 67
BbvCI CC'TCA_GC
Cuts at: 2324 2369 2324
Size: 45 6475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2120 5131 136
Size: 107 122 71 631 605 448 3011 1525
Fragments arranged by size:
3011 1525 631 605 448 122 107 71
BglII A'GATC_T
Cuts at: 3492 6515 3492
Size: 3023 3497
BlpI GC'TnA_GC
Cuts at: 3342 3937 3342
Size: 595 5925
Bme1580I G_kGCm'C
Cuts at: 1790 2640 2745 2794 4581 5737 1790
Size: 850 105 49 1787 1156 2573
Fragments arranged by size:
2573 1787 1156 850 105 49
BmgBI CAC'GTC
Cuts at: 1318 3276 1318
Size: 1958 4562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2271 4251 5209
Size: 416 53 83 399 24 854 1980 958
Cuts at: 5209 442
Size: 1753
Fragments arranged by size:
1980 1753 958 854 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2883 2883
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2440 2621 2737 2876 3715 3736 5180
Size: 917 284 181 116 139 839 21 1444
Cuts at: 5180 1239
Size: 2579
Fragments arranged by size:
2579 1444 917 839 284 181 139 116
21
Bpu10I CC'TnA_GC
Cuts at: 2324 2369 2858 3078 2324
Size: 45 489 220 5766
Fragments arranged by size:
5766 489 220 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4645 5510 5808 4645
Size: 865 298 5357
Fragments arranged by size:
5357 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2242 3133 3570 5183 882
Size: 617 743 891 437 1613 2219
Fragments arranged by size:
2219 1613 891 743 617 437
BsaAI yAC'GTr
Cuts at: 493 1204 2829 4311 493
Size: 711 1625 1482 2702
Fragments arranged by size:
2702 1625 1482 711
BsaBI GATnn'nnATC
Cuts at: 6514 6514
Size: 6520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3774 4327 4709 5344
Size: 53 83 186 936 2241 553 382 635
Cuts at: 5344 275
Size: 1451
Fragments arranged by size:
2241 1451 936 635 553 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2463 2481 3653 4953 5351 5625 5772 6183 2463
Size: 18 1172 1300 398 274 147 411 2800
Fragments arranged by size:
2800 1300 1172 411 398 274 147 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6295
Fragments arranged by size:
6295 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2008 2200 1186
Size: 822 192 5506
Fragments arranged by size:
5506 822 192
BseYI C'CCAG_C
Cuts at: 2010 2744 5723 6219 2010
Size: 734 2979 496 2311
Fragments arranged by size:
2979 2311 734 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2527 2900 1163
Size: 573 791 373 4783
Fragments arranged by size:
4783 791 573 373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3970 4731 5759 1540
Size: 275 186 1969 761 1028 2301
Fragments arranged by size:
2301 1969 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 3269 3504 4581 4666 5737 2541
Size: 728 235 1077 85 1071 3324
Fragments arranged by size:
3324 1077 1071 728 235 85
BsmI GAATG_Cn'
Cuts at: 4031 4124 4031
Size: 93 6427
BsmBI CGTCTCn'nnnn_
Cuts at: 2711 2763 2711
Size: 52 6468
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2541 2559 2640 2745 2794 3011 3111
Size: 399 751 18 81 105 49 217 100
Cuts at: 3111 3269 3504 3839 4581 4666 5737 1391
Size: 158 235 335 742 85 1071 2174
Fragments arranged by size:
2174 1071 751 742 399 335 235 217
158 105 100 85 81 49 18
BspEI T'CCGG_A
Cuts at: 2463 3653 6183 2463
Size: 1190 2530 2800
Fragments arranged by size:
2800 2530 1190
BspHI T'CATG_A
Cuts at: 2835 4409 6139 2835
Size: 1574 1730 3216
Fragments arranged by size:
3216 1730 1574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2385 2922 3789 3875 844
Size: 1541 537 867 86 3489
Fragments arranged by size:
3489 1541 867 537 86
BsrBI CCG'CTC
Cuts at: 1394 1823 2305 2751 4407 1394
Size: 429 482 446 1656 3507
Fragments arranged by size:
3507 1656 482 446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2935 3336 5015 5189 65
Size: 1183 1687 401 1679 174 1396
Fragments arranged by size:
1687 1679 1396 1183 401 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2161 5164 5351 1700
Size: 110 351 3003 187 2869
Fragments arranged by size:
3003 2869 351 187 110
BsrGI T'GTAC_A
Cuts at: 96 3278 96
Size: 3182 3338
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 2658 2945 4580 5592 2658
Size: 287 1635 1012 3586
Fragments arranged by size:
3586 1635 1012 287
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6520
BstBI TT'CG_AA
Cuts at: 2812 5324 2812
Size: 2512 4008
BstEII G'GTnAC_C
Cuts at: 1184 2404 1184
Size: 1220 5300
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 3001 2500
Size: 501 6019
BstYI r'GATC_y
Cuts at: 1972 3030 3241 3492 3531 3850 4217 4602 4619
Size: 1058 211 251 39 319 367 385 17
Cuts at: 4619 6071 6157 6169 6515 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1058 385 367 346 319 251
211 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 3357 3357
Size: 6520
BtgI C'CryG_G
Cuts at: 513 1065 2920 3663 513
Size: 552 1855 743 3370
Fragments arranged by size:
3370 1855 743 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5781
Fragments arranged by size:
5781 683 56
BtsI GCAGTG_nn'
Cuts at: 2323 2629 2858 3329 4036 4830 4850 2323
Size: 306 229 471 707 794 20 3993
Fragments arranged by size:
3993 794 707 471 306 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DraIII CAC_nnn'GTG
Cuts at: 2712 2712
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3734 5527 809
Size: 507 2418 1793 1802
Fragments arranged by size:
2418 1802 1793 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2698 3670 3798
Size: 54 1425 50 275 18 868 972 128
Cuts at: 3798 3967 4856 6485 8
Size: 169 889 1629 43
Fragments arranged by size:
1629 1425 972 889 868 275 169 128
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3967 1537
Size: 275 2155 4090
Fragments arranged by size:
4090 2155 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2761 2946 3392 3791 4450 1353
Size: 1408 185 446 399 659 3423
Fragments arranged by size:
3423 1408 659 446 399 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5107 5481 5627 387
Size: 1064 138 3518 374 146 1280
Fragments arranged by size:
3518 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 3267 2539
Size: 728 5792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2156 2440 2621 2737 2876 3530
Size: 393 416 108 284 181 116 139 654
Cuts at: 3530 3715 3736 4583 5180 5967 6235 1239
Size: 185 21 847 597 787 268 1524
Fragments arranged by size:
1524 847 787 654 597 416 393 284
268 185 181 139 116 108 21
EcoO109I rG'GnC_Cy
Cuts at: 2647 2734 2911 3322 2647
Size: 87 177 411 5845
Fragments arranged by size:
5845 411 177 87
EcoRI G'AATT_C
Cuts at: 2779 3829 4207 2779
Size: 1050 378 5092
Fragments arranged by size:
5092 1050 378
FspI TGC'GCA
Cuts at: 3764 5026 3764
Size: 1262 5258
FspAI rTGC'GCAy
Cuts at: 3764 3764
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 2035 5667 1678
Size: 357 3632 2531
Fragments arranged by size:
3632 2531 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 2417 2449 3196
Size: 32 135 32 669 32 144 32 747
Cuts at: 3196 3226 3228 3258 5161 5193 5235 5267 1373
Size: 30 2 30 1903 32 42 32 2626
Fragments arranged by size:
2626 1903 747 669 144 135 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 3864 4110 669
Size: 988 281 655 1271 246 3079
Fragments arranged by size:
3079 1271 988 655 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6520
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3174 5634 5818 1305
Size: 1869 2460 184 2007
Fragments arranged by size:
2460 2007 1869 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3672 3800 10
Size: 54 1425 343 1840 128 2730
Fragments arranged by size:
2730 1840 1425 343 128 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3743 4478 4837 4996 518
Size: 648 2577 735 359 159 2042
Fragments arranged by size:
2577 2042 735 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2328 2337 2574 3936 4613 5761 6006 1606
Size: 722 9 237 1362 677 1148 245 2120
Fragments arranged by size:
2120 1362 1148 722 677 245 237 9
NcoI C'CATG_G
Cuts at: 513 1065 3663 513
Size: 552 2598 3370
Fragments arranged by size:
3370 2598 552
NdeI CA'TA_TG
Cuts at: 387 2978 387
Size: 2591 3929
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 2908 3432 3878 5423 1132
Size: 97 1679 524 446 1545 2229
Fragments arranged by size:
2229 1679 1545 524 446 97
PasI CC'CwG_GG
Cuts at: 1348 2738 2794 1348
Size: 1390 56 5074
Fragments arranged by size:
5074 1390 56
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2422 2800 1108
Size: 1314 378 4828
Fragments arranged by size:
4828 1314 378
PfoI T'CCnGG_A
Cuts at: 2217 3527 2217
Size: 1310 5210
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PmlI CAC'GTG
Cuts at: 2829 2829
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4589 4621 6102 6134 4589
Size: 32 1481 32 4975
Fragments arranged by size:
4975 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2911 3322 2911
Size: 411 6109
PshAI GACnn'nnGTC
Cuts at: 1613 3145 1613
Size: 1532 4988
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PspOMI G'GGCC_C
Cuts at: 2741 2741
Size: 6520
PstI C_TGCA'G
Cuts at: 830 2553 2853 3872 830
Size: 1723 300 1019 3478
Fragments arranged by size:
3478 1723 1019 300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 2574 2574
Size: 6520
SacI G_AGCT'C
Cuts at: 2541 3269 2541
Size: 728 5792
SalI G'TCGA_C
Cuts at: 1936 2591 3862 1936
Size: 655 1271 4594
Fragments arranged by size:
4594 1271 655
SapI GCTCTTCn'nnn_
Cuts at: 3392 3791 3392
Size: 399 6121
SbfI CC_TGCA'GG
Cuts at: 2553 3872 2553
Size: 1319 5201
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SexAI A'CCwGG_T
Cuts at: 2586 2586
Size: 6520
SfcI C'TryA_G
Cuts at: 826 1045 2549 2849 3868 5003 5684 5875 826
Size: 219 1504 300 1019 1135 681 191 1471
Fragments arranged by size:
1504 1471 1135 1019 681 300 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 2294 2447 2763 3847 1872
Size: 422 153 316 1084 4545
Fragments arranged by size:
4545 1084 422 316 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3586 4624 5525 5787 820
Size: 197 926 1643 1038 901 262 1553
Fragments arranged by size:
1643 1553 1038 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6520
StyI C'CwwG_G
Cuts at: 513 1065 2818 3663 3959 513
Size: 552 1753 845 296 3074
Fragments arranged by size:
3074 1753 845 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3656 4547 4564 4717 4902 5347 325
Size: 1255 2076 891 17 153 185 445 1498
Fragments arranged by size:
2076 1498 1255 891 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3278 4766 6478
Size: 274 80 33 51 1443 1301 1488 1712
Cuts at: 6478 96
Size: 138
Fragments arranged by size:
1712 1488 1443 1301 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6480 6512 6480
Size: 32 6488
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6520
XbaI T'CTAG_A
Cuts at: 3214 3856 3214
Size: 642 5878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2801 2801
Size: 6520
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 2292 2445 2761 3845 1870
Size: 422 153 316 1084 4545
Fragments arranged by size:
4545 1084 422 316 153
XmnI GAAnn'nnTTC
Cuts at: 3515 4649 3515
Size: 1134 5386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoNI EcoRV FalI FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI