Restriction map for
pFN21AE3623
(Circular) (Six-base) MAPSORT of: pFN21AE3623.seq Check: 954 from: 1 to: 5149
pFN21AE3623 5149 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2163 2959 278
Size: 53 83 186 936 627 796 2468
Fragments arranged by size:
2468 936 796 627 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2492 1937
Size: 555 4594
Acc65I G'GTAC_C
Cuts at: 2470 2470
Size: 5149
AclI AA'CG_TT
Cuts at: 1563 3276 3649 1563
Size: 1713 373 3063
Fragments arranged by size:
3063 1713 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3212 4596 4864 1632
Size: 1580 1384 268 1917
Fragments arranged by size:
1917 1580 1384 268
AfeI AGC'GCT
Cuts at: 2019 2019
Size: 5149
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4952
AflIII A'CryG_T
Cuts at: 1203 4048 5025 1203
Size: 2845 977 1327
Fragments arranged by size:
2845 1327 977
AgeI A'CCGG_T
Cuts at: 3980 3980
Size: 5149
AhdI GACnn_n'nnGTC
Cuts at: 3878 3878
Size: 5149
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2101 1184
Size: 917 4232
AlwNI CAG_nnn'CTG
Cuts at: 2892 4464 2892
Size: 1572 3577
ApaLI G'TGCA_C
Cuts at: 3206 4362 3206
Size: 1156 3993
ApoI r'AATT_y
Cuts at: 1493 2458 2684 2836 4945 1493
Size: 965 226 152 2109 1697
Fragments arranged by size:
2109 1697 965 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3703 160
Size: 872 2671 1606
Fragments arranged by size:
2671 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5149
AvaI C'yCGr_G
Cuts at: 1870 1943 2474 1870
Size: 73 531 4545
Fragments arranged by size:
4545 531 73
BamHI G'GATC_C
Cuts at: 2479 2846 2479
Size: 367 4782
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 2470 3925 618
Size: 325 217 627 238 445 1455 1842
Fragments arranged by size:
1842 1455 627 445 325 238 217
BanII G_rGCy'C
Cuts at: 1391 2468 1391
Size: 1077 4072
BbeI G_GCGC'C
Cuts at: 2029 2029
Size: 5149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3340 3374 1903
Size: 34 1403 34 3678
Fragments arranged by size:
3678 1403 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3041 4251 3041
Size: 1210 3939
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5149
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3760 136
Size: 107 122 71 631 605 2088 1525
Fragments arranged by size:
2088 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5144 5144
Size: 5149
BlpI GC'TnA_GC
Cuts at: 2566 2566
Size: 5149
Bme1580I G_kGCm'C
Cuts at: 1790 3210 4366 1790
Size: 1420 1156 2573
Fragments arranged by size:
2573 1420 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5149
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2880 3838 442
Size: 416 53 83 399 24 1463 958 1753
Fragments arranged by size:
1753 1463 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 3809 1239
Size: 795 1775 2579
Fragments arranged by size:
2579 1775 795
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3274 4139 4437 3274
Size: 865 298 3986
Fragments arranged by size:
3986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3812 882
Size: 617 2313 2219
Fragments arranged by size:
2313 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2940 493
Size: 711 1736 2702
Fragments arranged by size:
2702 1736 711
BsaBI GATnn'nnATC
Cuts at: 5143 5143
Size: 5149
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 2160 2956 3338
Size: 53 83 186 936 493 134 796 382
Cuts at: 3338 3973 275
Size: 635 1451
Fragments arranged by size:
1451 936 796 635 493 382 186 134
83 53
BsaWI w'CCGG_w
Cuts at: 3582 3980 4254 4401 4812 3582
Size: 398 274 147 411 3919
Fragments arranged by size:
3919 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4924
Fragments arranged by size:
4924 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5149
BseYI C'CCAG_C
Cuts at: 4352 4848 4352
Size: 496 4653
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2203 2299 1163
Size: 573 467 96 4013
Fragments arranged by size:
4013 573 467 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2599 3360 4388 1540
Size: 275 186 598 761 1028 2301
Fragments arranged by size:
2301 1028 761 598 275 186
BsiHKAI G_wGCw'C
Cuts at: 3210 3295 4366 3210
Size: 85 1071 3993
Fragments arranged by size:
3993 1071 85
BsmI GAATG_Cn'
Cuts at: 2660 2753 2660
Size: 93 5056
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2468 3210 3295 4366 1391
Size: 399 678 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 678 399 85
BspEI T'CCGG_A
Cuts at: 4812 4812
Size: 5149
BspHI T'CATG_A
Cuts at: 3038 4768 3038
Size: 1730 3419
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2504 844
Size: 1660 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3036 1394
Size: 429 1213 3507
Fragments arranged by size:
3507 1213 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3644 3818 65
Size: 1183 2396 174 1396
Fragments arranged by size:
2396 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2285 3793 3980 1700
Size: 110 475 1508 187 2869
Fragments arranged by size:
2869 1508 475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5149
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5149
BssSI C'ACGA_G
Cuts at: 3209 4221 3209
Size: 1012 4137
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5149
BstBI TT'CG_AA
Cuts at: 3953 3953
Size: 5149
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5149
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2266 2242
Size: 24 5125
BstYI r'GATC_y
Cuts at: 1972 2479 2846 3231 3248 4700 4786 4798 5144
Size: 507 367 385 17 1452 86 12 346
Cuts at: 5144 1972
Size: 1977
Fragments arranged by size:
1977 1452 507 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2319 2400 513
Size: 552 939 315 81 3262
Fragments arranged by size:
3262 939 552 315 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4410
Fragments arranged by size:
4410 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 3459 3479 2665
Size: 794 20 4335
Fragments arranged by size:
4335 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5114
DraI TTT'AAA
Cuts at: 2453 2453
Size: 5149
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4156 809
Size: 507 2840 1802
Fragments arranged by size:
2840 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2596 3485 5114
Size: 54 1425 50 275 18 766 889 1629
Cuts at: 5114 8
Size: 43
Fragments arranged by size:
1629 1425 889 766 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2596 1537
Size: 275 784 4090
Fragments arranged by size:
4090 784 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3079 1353
Size: 1726 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3736 4110 4256 387
Size: 1064 138 2147 374 146 1280
Fragments arranged by size:
2147 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 3212 3809 4596 4864 1239
Size: 393 402 1178 597 787 268 1524
Fragments arranged by size:
1524 1178 787 597 402 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2270 2270
Size: 5149
EcoO109I rG'GnC_Cy
Cuts at: 2341 2341
Size: 5149
EcoRI G'AATT_C
Cuts at: 2458 2836 2458
Size: 378 4771
FspI TGC'GCA
Cuts at: 3655 3655
Size: 5149
HaeII r_GCGC'y
Cuts at: 1678 2021 2029 4296 1678
Size: 343 8 2267 2531
Fragments arranged by size:
2531 2267 343 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3790 3822 3864 3896 1373
Size: 32 135 32 2218 32 42 32 2626
Fragments arranged by size:
2626 2218 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2493 2739 669
Size: 988 281 555 246 3079
Fragments arranged by size:
3079 988 555 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5149
HpaI GTT'AAC
Cuts at: 2739 2739
Size: 5149
KasI G'GCGC_C
Cuts at: 2025 2025
Size: 5149
KpnI G_GTAC'C
Cuts at: 2474 2474
Size: 5149
MfeI C'AATT_G
Cuts at: 2748 2748
Size: 5149
MluI A'CGCG_T
Cuts at: 5025 5025
Size: 5149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 2334 4263 4447 1305
Size: 780 249 1929 184 2007
Fragments arranged by size:
2007 1929 780 249 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3327
Fragments arranged by size:
3327 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 3107 3466 3625 518
Size: 648 1059 882 359 159 2042
Fragments arranged by size:
2042 1059 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2368 2565 3242 4390 4635 1606
Size: 762 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 245 197
NarI GG'CG_CC
Cuts at: 2026 2026
Size: 5149
NcoI C'CATG_G
Cuts at: 513 1065 2004 2400 513
Size: 552 939 396 3262
Fragments arranged by size:
3262 939 552 396
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5149
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5149
NotI GC'GGCC_GC
Cuts at: 2596 2596
Size: 5149
NspI r_CATG'y
Cuts at: 1132 1229 2104 2507 4052 1132
Size: 97 875 403 1545 2229
Fragments arranged by size:
2229 1545 875 403 97
PasI CC'CwG_GG
Cuts at: 1348 2272 1348
Size: 924 4225
PciI A'CATG_T
Cuts at: 4048 4048
Size: 5149
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5149
PmeI GTTT'AAAC
Cuts at: 2453 2453
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 4731 4763 3218
Size: 32 1481 32 3604
Fragments arranged by size:
3604 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 2341
Size: 5149
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5149
PsiI TTA'TAA
Cuts at: 2719 2719
Size: 5149
PstI C_TGCA'G
Cuts at: 830 2501 830
Size: 1671 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5149
SalI G'TCGA_C
Cuts at: 1936 2491 1936
Size: 555 4594
SbfI CC_TGCA'GG
Cuts at: 2501 2501
Size: 5149
ScaI AGT'ACT
Cuts at: 3397 5109 3397
Size: 1712 3437
SfcI C'TryA_G
Cuts at: 826 1045 2497 3632 4313 4504 826
Size: 219 1452 1135 681 191 1471
Fragments arranged by size:
1471 1452 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2027 2027
Size: 5149
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5149
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5149
SmaI CCC'GGG
Cuts at: 1872 2476 1872
Size: 604 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3253 4154 4416 820
Size: 197 926 1310 901 262 1553
Fragments arranged by size:
1553 1310 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2940 493
Size: 2447 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5149
SphI G_CATG'C
Cuts at: 1132 2507 1132
Size: 1375 3774
SspI AAT'ATT
Cuts at: 5 52 3073 5
Size: 47 3021 2081
Fragments arranged by size:
3021 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5149
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2588 513
Size: 552 939 396 188 3074
Fragments arranged by size:
3074 939 552 396 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2055 3176 3193 3346 3531 3976 325
Size: 1255 475 1121 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1121 475 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 3395 5107
Size: 274 80 33 51 1443 219 1199 1712
Cuts at: 5107 96
Size: 138
Fragments arranged by size:
1712 1443 1199 274 219 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5109 5141 5109
Size: 32 5117
Tth111I GACn'n_nGTC
Cuts at: 1316 2327 1316
Size: 1011 4138
XbaI T'CTAG_A
Cuts at: 2485 2485
Size: 5149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 2407 2317
Size: 90 5059
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5149
XmaI C'CCGG_G
Cuts at: 1870 2474 1870
Size: 604 4545
XmnI GAAnn'nnTTC
Cuts at: 2132 3278 2132
Size: 1146 4003
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2161 2957 276
Size: 53 83 186 936 627 796 2468
Fragments arranged by size:
2468 936 796 627 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI