Restriction map for
pFN21AE3622
(Circular) (Six-base) MAPSORT of: pFN21AE3622.seq Check: 7275 from: 1 to: 5830
pFN21AE3622 5830 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 3893 5277 5545 1632
Size: 593 1668 1384 268 1917
Fragments arranged by size:
1917 1668 1384 593 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 5798
AlwNI CAG_nnn'CTG
Cuts at: 3573 5145 3573
Size: 1572 4258
ApaI G_GGCC'C
Cuts at: 3104 3104
Size: 5830
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2923 3155 1870
Size: 73 142 838 232 4545
Fragments arranged by size:
4545 838 232 142 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 2262 2491 2909 2965
Size: 325 217 627 325 150 229 418 56
Cuts at: 2965 3151 4606 618
Size: 186 1455 1842
Fragments arranged by size:
1842 1455 627 418 325 325 229 217
186 150 56
BanII G_rGCy'C
Cuts at: 1391 3104 3149 1391
Size: 1713 45 4072
Fragments arranged by size:
4072 1713 45
BbeI G_GCGC'C
Cuts at: 2116 2969 2116
Size: 853 4977
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3109 3143 4021 4055 1903
Size: 34 1172 34 878 34 3678
Fragments arranged by size:
3678 1172 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2414 4441 136
Size: 107 122 71 631 605 742 2027 1525
Fragments arranged by size:
2027 1525 742 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2256 5825 2256
Size: 3569 2261
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2496 3104 3891 5047 1790
Size: 706 608 787 1156 2573
Fragments arranged by size:
2573 1156 787 706 608
BmgBI CAC'GTC
Cuts at: 1318 2863 1318
Size: 1545 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2248 2303 3561
Size: 416 53 83 399 24 831 55 1258
Cuts at: 3561 4519 442
Size: 958 1753
Fragments arranged by size:
1753 1258 958 831 416 399 83 55
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2490 2732 4490 1239
Size: 1251 242 1758 2579
Fragments arranged by size:
2579 1758 1251 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 2825 4493 882
Size: 617 873 453 1668 2219
Fragments arranged by size:
2219 1668 873 617 453
BsaAI yAC'GTr
Cuts at: 493 1204 2164 2404 3621 493
Size: 711 960 240 1217 2702
Fragments arranged by size:
2702 1217 960 711 240
BsaBI GATnn'nnATC
Cuts at: 2987 5824 2987
Size: 2837 2993
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2113 2130 2966 3637
Size: 53 83 186 936 580 17 836 671
Cuts at: 3637 4019 4654 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 836 671 635 580 382 186
83 53 17
BsaWI w'CCGG_w
Cuts at: 2769 2776 4263 4661 4935 5082 5493 2769
Size: 7 1487 398 274 147 411 3106
Fragments arranged by size:
3106 1487 411 398 274 147 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2463 2493 2636
Size: 30 165 30 624 30 406 30 143
Cuts at: 2636 2666 2771 2801 1178
Size: 30 105 30 4207
Fragments arranged by size:
4207 624 406 165 143 105 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 2692 1186
Size: 1129 377 4324
Fragments arranged by size:
4324 1129 377
BseYI C'CCAG_C
Cuts at: 2069 2969 5033 5529 2069
Size: 900 2064 496 2370
Fragments arranged by size:
2370 2064 900 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2616 3280 4041 5069 1540
Size: 275 186 615 664 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 615 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 3891 3976 5047 2307
Size: 1584 85 1071 3090
Fragments arranged by size:
3090 1584 1071 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
BsmBI CGTCTCn'nnnn_
Cuts at: 2466 2466
Size: 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2496 3104 3149 3891 3976 5047
Size: 399 517 189 608 45 742 85 1071
Cuts at: 5047 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 608 517 399 189 85
45
BspEI T'CCGG_A
Cuts at: 2776 5493 2776
Size: 2717 3113
BspHI T'CATG_A
Cuts at: 2571 2850 3719 5449 2571
Size: 279 869 1730 2952
Fragments arranged by size:
2952 1730 869 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2620 2764 3717 1394
Size: 429 797 144 953 3507
Fragments arranged by size:
3507 953 797 429 144
BsrDI GCAATG_nn'
Cuts at: 65 1248 2764 2928 4325 4499 65
Size: 1183 1516 164 1397 174 1396
Fragments arranged by size:
1516 1397 1396 1183 174 164
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 2828 3890 4902 2828
Size: 1062 1012 3756
Fragments arranged by size:
3756 1062 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2311 794
Size: 1517 4313
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 4535
BstXI CCAn_nnnn'nTGG
Cuts at: 2404 2404
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2256 2577 3160 3527 3912 3929 5381 5467
Size: 284 321 583 367 385 17 1452 86
Cuts at: 5467 5479 5825 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 583 385 367 346 321 284
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2610 513
Size: 552 939 606 3733
Fragments arranged by size:
3733 939 606 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2977 487
Size: 683 56 1751 3340
Fragments arranged by size:
3340 1751 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4140 4160 3346
Size: 794 20 5016
Fragments arranged by size:
5016 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2437 2472 529
Size: 35 1873 35 3887
Fragments arranged by size:
3887 1873 35 35
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DraIII CAC_nnn'GTG
Cuts at: 2311 2491 2311
Size: 180 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2394 2406 2548
Size: 54 1425 50 275 18 564 12 142
Cuts at: 2548 2613 2685 2737 3277 4166 5795 8
Size: 65 72 52 540 889 1629 43
Fragments arranged by size:
1629 1425 889 564 540 275 142 72
65 54 52 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2613 3277 1537
Size: 275 801 664 4090
Fragments arranged by size:
4090 801 664 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 2710 2711 3760 1353
Size: 654 703 1 1049 3423
Fragments arranged by size:
3423 1049 703 654 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2490 2732 3893 4490 5277 5545
Size: 393 593 265 242 1161 597 787 268
Cuts at: 5545 1239
Size: 1524
Fragments arranged by size:
1524 1161 787 597 593 393 268 265
242
EcoO109I rG'GnC_Cy
Cuts at: 2326 2554 3101 2326
Size: 228 547 5055
Fragments arranged by size:
5055 547 228
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2116 2786 2969 4977 1678
Size: 438 670 183 2008 2531
Fragments arranged by size:
2531 2008 670 438 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2463 2495 4471
Size: 32 135 32 455 32 404 32 1976
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1976 455 404 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KasI G'GCGC_C
Cuts at: 2112 2965 2112
Size: 853 4977
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 2687 2739 10
Size: 54 1425 343 576 279 52 3101
Fragments arranged by size:
3101 1425 576 343 279 54 52
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2459 2915 3788 4147 4306 518
Size: 648 1236 57 456 873 359 159 2042
Fragments arranged by size:
2042 1236 873 648 456 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 3246 3923 5071 5316 1606
Size: 1640 677 1148 245 2120
Fragments arranged by size:
2120 1640 1148 677 245
NarI GG'CG_CC
Cuts at: 2113 2966 2113
Size: 853 4977
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4339
Fragments arranged by size:
4339 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2053 3188 4733 1132
Size: 97 824 1135 1545 2229
Fragments arranged by size:
2229 1545 1135 824 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 3899 3931 5412 5444 2771
Size: 32 1096 32 1481 32 3157
Fragments arranged by size:
3157 1481 1096 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2554 2554
Size: 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspOMI G'GGCC_C
Cuts at: 3100 3100
Size: 5830
PstI C_TGCA'G
Cuts at: 830 3182 830
Size: 2352 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2711 2711
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 3014 4078 5790 3014
Size: 1064 1712 3054
Fragments arranged by size:
3054 1712 1064
SfcI C'TryA_G
Cuts at: 826 1045 2596 3178 4313 4994 5185 826
Size: 219 1551 582 1135 681 191 1471
Fragments arranged by size:
1551 1471 1135 681 582 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2414 2414
Size: 5830
SfoI GGC'GCC
Cuts at: 2114 2967 2114
Size: 853 4977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 2925 3157 1872
Size: 1053 232 4545
Fragments arranged by size:
4545 1053 232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3934 4835 5097 820
Size: 197 926 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 3269 513
Size: 552 939 336 929 3074
Fragments arranged by size:
3074 939 929 552 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2322 2336 2498 2576 3857 3874 4027
Size: 1255 742 14 162 78 1281 17 153
Cuts at: 4027 4212 4657 325
Size: 185 445 1498
Fragments arranged by size:
1498 1281 1255 742 445 185 162 153
78 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3012 4076 5788
Size: 274 80 33 51 1443 1035 1064 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1712 1443 1064 1035 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2636 2668 5790 5822 2636
Size: 32 3122 32 2644
Fragments arranged by size:
3122 2644 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2477 1316
Size: 1161 4669
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2186 2533 2186
Size: 347 5483
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 2923 3155 1870
Size: 1053 232 4545
Fragments arranged by size:
4545 1053 232
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SrfI SwaI