Restriction map for
pFN21AE3620
(Circular) (Six-base) MAPSORT of: pFN21AE3620.seq Check: 2268 from: 1 to: 5374
pFN21AE3620 5374 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2457 3184 278
Size: 53 83 186 936 921 727 2468
Fragments arranged by size:
2468 936 921 727 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 2457
Size: 32 5342
AlwNI CAG_nnn'CTG
Cuts at: 2507 3117 4689 2507
Size: 610 1572 3192
Fragments arranged by size:
3192 1572 610
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2050 2699 1870
Size: 73 93 14 649 4545
Fragments arranged by size:
4545 649 93 73 14
AvrII C'CTAG_G
Cuts at: 2276 2276
Size: 5374
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2695 4150 618
Size: 325 217 627 273 635 1455 1842
Fragments arranged by size:
1842 1455 635 627 325 273 217
BanII G_rGCy'C
Cuts at: 1391 2693 1391
Size: 1302 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2486 2520 3565 3599 1903
Size: 34 549 34 1045 34 3678
Fragments arranged by size:
3678 1045 549 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 2208 2331 1548
Size: 660 123 4591
Fragments arranged by size:
4591 660 123
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3985 136
Size: 107 122 71 631 605 2313 1525
Fragments arranged by size:
2313 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2063 3435 4591 1790
Size: 273 1372 1156 2573
Fragments arranged by size:
2573 1372 1156 273
BmgBI CAC'GTC
Cuts at: 1318 2533 1318
Size: 1215 4159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 4063 442
Size: 416 53 83 399 24 1688 958 1753
Fragments arranged by size:
1753 1688 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2591 2591
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2092 4034 1239
Size: 842 11 1942 2579
Fragments arranged by size:
2579 1942 842 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 4037 882
Size: 617 600 1938 2219
Fragments arranged by size:
2219 1938 617 600
BsaAI yAC'GTr
Cuts at: 493 1204 3165 493
Size: 711 1961 2702
Fragments arranged by size:
2702 1961 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2454 3181 3563 4198
Size: 53 83 186 936 921 727 382 635
Cuts at: 4198 275
Size: 1451
Fragments arranged by size:
1451 936 921 727 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2588 3807 4205 4479 4626 5037 2588
Size: 1219 398 274 147 411 2925
Fragments arranged by size:
2925 1219 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5149
Fragments arranged by size:
5149 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2583 1186
Size: 1397 3977
BseYI C'CCAG_C
Cuts at: 4577 5073 4577
Size: 496 4878
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4801
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2577 3435 3520 4591 2577
Size: 858 85 1071 3360
Fragments arranged by size:
3360 1071 858 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
BsmBI CGTCTCn'nnnn_
Cuts at: 2375 2591 2375
Size: 216 5158
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2577 2693 3435 3520 4591 1391
Size: 399 273 514 116 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 399 273 116 85
BspEI T'CCGG_A
Cuts at: 2588 5037 2588
Size: 2449 2925
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 844
Size: 1885 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4018 4205 1700
Size: 110 2208 187 2869
Fragments arranged by size:
2869 2208 187 110
BsrGI T'GTAC_A
Cuts at: 96 2437 96
Size: 2341 3033
BssHII G'CGCG_C
Cuts at: 1925 2023 1925
Size: 98 5276
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2067 2507 794
Size: 1273 440 3661
Fragments arranged by size:
3661 1273 440
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstXI CCAn_nnnn'nTGG
Cuts at: 2090 2090
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2323 2704 3071 3456 3473 4925 5011 5023
Size: 351 381 367 385 17 1452 86 12
Cuts at: 5023 5369 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 381 367 351 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3883
Fragments arranged by size:
3883 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3684 3704 2890
Size: 794 20 4560
Fragments arranged by size:
4560 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DraIII CAC_nnn'GTG
Cuts at: 2067 2067
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2248 2821
Size: 54 1425 50 275 18 283 135 573
Cuts at: 2821 3710 5339 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 573 283 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3304 1353
Size: 1951 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2092 3437 4034 4821 5089 1239
Size: 393 449 11 1345 597 787 268 1524
Fragments arranged by size:
1524 1345 787 597 449 393 268 11
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 4521 1678
Size: 2843 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2377 2409 4015 4047 4089
Size: 32 135 32 805 32 1606 32 42
Cuts at: 4089 4121 1373
Size: 32 2626
Fragments arranged by size:
2626 1606 805 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 2363 4488 4672 1305
Size: 909 149 2125 184 2007
Fragments arranged by size:
2125 2007 909 184 149
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2250 10
Size: 54 1425 343 418 3134
Fragments arranged by size:
3134 1425 418 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2439 3332 3691 3850 518
Size: 648 1273 893 359 159 2042
Fragments arranged by size:
2042 1273 893 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 2790 3467 4615 4860 1606
Size: 506 678 677 1148 245 2120
Fragments arranged by size:
2120 1148 678 677 506 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3883
Fragments arranged by size:
3883 939 552
NdeI CA'TA_TG
Cuts at: 387 2671 387
Size: 2284 3090
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5374
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2726 830
Size: 1896 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2722 3857 4538 4729 826
Size: 219 1677 1135 681 191 1471
Fragments arranged by size:
1677 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2701 1872
Size: 829 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4379 4641 820
Size: 197 926 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 2344 3298 5
Size: 47 2292 954 2081
Fragments arranged by size:
2292 2081 954 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2004 2276 2813 513
Size: 552 939 272 537 3074
Fragments arranged by size:
3074 939 552 537 272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 3418 3571 3756 4201 325
Size: 1255 1821 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2437 2523 3620
Size: 274 80 33 51 1443 460 86 1097
Cuts at: 3620 5332 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1097 460 274 138 86 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2570 2602 5334 5366 2570
Size: 32 2732 32 2578
Fragments arranged by size:
2732 2578 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2699 1870
Size: 829 4545
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2455 3182 276
Size: 53 83 186 936 921 727 2468
Fragments arranged by size:
2468 936 921 727 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI