Restriction map for
pFN21AE3610
(Circular) (Six-base) MAPSORT of: pFN21AE3610.seq Check: 8808 from: 1 to: 5797
pFN21AE3610 5797 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2679 3860 5244 5512 1632
Size: 1047 1181 1384 268 1917
Fragments arranged by size:
1917 1384 1181 1047 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2896 1184
Size: 1712 4085
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaI G_GGCC'C
Cuts at: 2403 2403
Size: 5797
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 2163 2295 2593 2647 3106 3332 3484 5593
Size: 670 132 298 54 459 226 152 2109
Cuts at: 5593 1493
Size: 1697
Fragments arranged by size:
2109 1697 670 459 298 226 152 132
54
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 3122 1870
Size: 73 262 917 4545
Fragments arranged by size:
4545 917 262 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 3118 4573 618
Size: 325 217 627 329 1002 1455 1842
Fragments arranged by size:
1842 1455 1002 627 329 325 217
BanII G_rGCy'C
Cuts at: 1391 2403 3116 1391
Size: 1012 713 4072
Fragments arranged by size:
4072 1012 713
BbeI G_GCGC'C
Cuts at: 2120 2120
Size: 5797
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2667 3072 928
Size: 971 768 405 3653
Fragments arranged by size:
3653 971 768 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2196 2230 3988 4022 1903
Size: 34 50 34 175 34 1758 34 3678
Fragments arranged by size:
3678 1758 175 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3002 3689 4899 3002
Size: 687 1210 3900
Fragments arranged by size:
3900 1210 687
BclI T'GATC_A
Cuts at: 1548 2725 1548
Size: 1177 4620
BfrBI ATG'CAT
Cuts at: 2956 2956
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2403 3858 5014 1790
Size: 613 1455 1156 2573
Fragments arranged by size:
2573 1455 1156 613
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2205 3528 4486
Size: 416 53 83 399 24 788 1323 958
Cuts at: 4486 442
Size: 1753
Fragments arranged by size:
1753 1323 958 788 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2630 4457 1239
Size: 1391 1827 2579
Fragments arranged by size:
2579 1827 1391
Bpu10I CC'TnA_GC
Cuts at: 2487 2565 2487
Size: 78 5719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2623 3922 4787 5085 2623
Size: 1299 865 298 3335
Fragments arranged by size:
3335 1299 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2206 4460 882
Size: 617 707 2254 2219
Fragments arranged by size:
2254 2219 707 617
BsaAI yAC'GTr
Cuts at: 493 1204 2221 3588 493
Size: 711 1017 1367 2702
Fragments arranged by size:
2702 1367 1017 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2117 2235 3604 3986
Size: 53 83 186 936 584 118 1369 382
Cuts at: 3986 4621 275
Size: 635 1451
Fragments arranged by size:
1451 1369 936 635 584 382 186 118
83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5572
Fragments arranged by size:
5572 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 1186
Size: 1132 4665
BseYI C'CCAG_C
Cuts at: 2230 2379 2383 5000 5496 2230
Size: 149 4 2617 496 2531
Fragments arranged by size:
2617 2531 496 149 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3858 3943 5014 3858
Size: 85 1071 4641
Fragments arranged by size:
4641 1071 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 3116 3858 3943 5014 1391
Size: 399 613 713 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 713 613 399 85
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2559 3152 844
Size: 1715 593 3489
Fragments arranged by size:
3489 1715 593
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2850 4292 4466 65
Size: 1183 1602 1442 174 1396
Fragments arranged by size:
1602 1442 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4441 4628 1700
Size: 110 2631 187 2869
Fragments arranged by size:
2869 2631 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 2973 3127 3494 3879 3896 5348 5434 5446
Size: 1001 154 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1001 385 367 346 154 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2239 2522 2884 513
Size: 552 1174 283 362 3426
Fragments arranged by size:
3426 1174 552 362 283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 3313 4107 4127 3313
Size: 794 20 4983
Fragments arranged by size:
4983 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DraIII CAC_nnn'GTG
Cuts at: 2534 2534
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3074 4804 809
Size: 507 1758 1730 1802
Fragments arranged by size:
1802 1758 1730 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2525 3244 4133
Size: 54 1425 50 275 18 695 719 889
Cuts at: 4133 5762 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 719 695 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3727 1353
Size: 2374 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2630 2679 3860 4457 5244 5512 1239
Size: 393 998 49 1181 597 787 268 1524
Fragments arranged by size:
1524 1181 998 787 597 393 268 49
EcoNI CCTnn'n_nnAGG
Cuts at: 2453 2490 2453
Size: 37 5760
EcoO109I rG'GnC_Cy
Cuts at: 2399 2827 2399
Size: 428 5369
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2120 4944 1678
Size: 442 2824 2531
Fragments arranged by size:
2824 2531 442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 2745 2777 2899
Size: 32 135 32 1049 32 92 32 122
Cuts at: 2899 2931 4438 4470 4512 4544 1373
Size: 32 1507 32 42 32 2626
Fragments arranged by size:
2626 1507 1049 135 122 92 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2370 2965 3141 3387 669
Size: 988 281 432 595 176 246 3079
Fragments arranged by size:
3079 988 595 432 281 246 176
HindIII A'AGCT_T
Cuts at: 748 2304 2774 748
Size: 1556 470 3771
Fragments arranged by size:
3771 1556 470
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KasI G'GCGC_C
Cuts at: 2116 2116
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2161 4911 5095 1305
Size: 856 2750 184 2007
Fragments arranged by size:
2750 2007 856 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2527 10
Size: 54 1425 343 695 3280
Fragments arranged by size:
3280 1425 695 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 3755 4114 4273 518
Size: 648 1851 738 359 159 2042
Fragments arranged by size:
2042 1851 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2241 2383 3213 3890 5038 5283 1606
Size: 635 142 830 677 1148 245 2120
Fragments arranged by size:
2120 1148 830 677 635 245 142
NarI GG'CG_CC
Cuts at: 2117 2117
Size: 5797
NcoI C'CATG_G
Cuts at: 513 1065 2522 2884 513
Size: 552 1457 362 3426
Fragments arranged by size:
3426 1457 552 362
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NruI TCG'CGA
Cuts at: 2178 2178
Size: 5797
NsiI A_TGCA'T
Cuts at: 2958 2958
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2960 3155 4700 1132
Size: 97 1731 195 1545 2229
Fragments arranged by size:
2229 1731 1545 195 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2101 1108
Size: 993 4804
PfoI T'CCnGG_A
Cuts at: 2169 2607 2823 2169
Size: 438 216 5143
Fragments arranged by size:
5143 438 216
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2827 2827
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2399 2399
Size: 5797
PstI C_TGCA'G
Cuts at: 830 2322 3149 830
Size: 1492 827 3478
Fragments arranged by size:
3478 1492 827
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2383 2383
Size: 5797
SacII CC_GC'GG
Cuts at: 2242 2242
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2318 3145 4280 4961 5152 826
Size: 219 1273 827 1135 681 191 1471
Fragments arranged by size:
1471 1273 1135 827 681 219 191
SfoI GGC'GCC
Cuts at: 2118 2118
Size: 5797
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2638 3901 4802 5064 820
Size: 197 926 695 1263 901 262 1553
Fragments arranged by size:
1553 1263 926 901 695 262 197
SnaBI TAC'GTA
Cuts at: 493 2221 3588 493
Size: 1728 1367 2702
Fragments arranged by size:
2702 1728 1367
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 2960 3155 1132
Size: 1828 195 3774
Fragments arranged by size:
3774 1828 195
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2269 2522 2859 2884 3236 513
Size: 552 1204 253 337 25 352 3074
Fragments arranged by size:
3074 1204 552 352 337 253 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3078 3824 3841 3994 4179 4624 325
Size: 1255 1498 746 17 153 185 445 1498
Fragments arranged by size:
1498 1498 1255 746 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2248 4043 5755
Size: 274 80 33 51 1443 271 1795 1712
Cuts at: 5755 96
Size: 138
Fragments arranged by size:
1795 1712 1443 274 271 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 3044 1316
Size: 1728 4069
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 2866
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 2165 2594 3926 2165
Size: 429 1332 4036
Fragments arranged by size:
4036 1332 429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV PacI PmlI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI