Restriction map for
pFN21AE3606
(Circular) (Six-base) MAPSORT of: pFN21AE3606.seq Check: 2417 from: 1 to: 6142
pFN21AE3606 6142 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2083 2083
Size: 6142
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2646 3485 1937
Size: 709 839 4594
Fragments arranged by size:
4594 839 709
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3083 4205 5589 5857 1632
Size: 1451 1122 1384 268 1917
Fragments arranged by size:
1917 1451 1384 1122 268
AfeI AGC'GCT
Cuts at: 2372 2372
Size: 6142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2315
Size: 32 6110
AlwNI CAG_nnn'CTG
Cuts at: 2185 2254 2521 3885 5457 2185
Size: 69 267 1364 1572 2870
Fragments arranged by size:
2870 1572 1364 267 69
ApaI G_GGCC'C
Cuts at: 2044 2141 2200 2044
Size: 97 59 5986
Fragments arranged by size:
5986 97 59
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 3451 3677 3829 5938 1493
Size: 1958 226 152 2109 1697
Fragments arranged by size:
2109 1958 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2074 2099 2134 2199 2384 2985
Size: 73 90 41 25 35 65 185 601
Cuts at: 2985 3467 1870
Size: 482 4545
Fragments arranged by size:
4545 601 482 185 90 73 65 41
35 25
BamHI G'GATC_C
Cuts at: 2323 3472 3839 2323
Size: 1149 367 4626
Fragments arranged by size:
4626 1149 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 3463 4918 618
Size: 325 217 627 308 1368 1455 1842
Fragments arranged by size:
1842 1455 1368 627 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2021 2044 2075 2141 2156 2200 2405 2478
Size: 630 23 31 66 15 44 205 73
Cuts at: 2478 3461 1391
Size: 983 4072
Fragments arranged by size:
4072 983 630 205 73 66 44 31
23 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2446 3375 928
Size: 971 547 929 3695
Fragments arranged by size:
3695 971 929 547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2264 2273 2291
Size: 107 122 71 631 605 592 9 18
Cuts at: 2291 2360 2482 4753 136
Size: 69 122 2271 1525
Fragments arranged by size:
2271 1525 631 605 592 122 122 107
71 69 18 9
BglII A'GATC_T
Cuts at: 3148 3282 6137 3148
Size: 134 2855 3153
Fragments arranged by size:
3153 2855 134
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2100 2141 2200 4203 5359 1790
Size: 254 56 41 59 2003 1156 2573
Fragments arranged by size:
2573 2003 1156 254 59 56 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3007 3109 3873
Size: 416 53 83 399 24 1590 102 764
Cuts at: 3873 4831 442
Size: 958 1753
Fragments arranged by size:
1753 1590 958 764 416 399 102 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2389 2864 3100 4802 1239
Size: 1150 475 236 1702 2579
Fragments arranged by size:
2579 1702 1150 475 236
Bpu10I CC'TnA_GC
Cuts at: 2262 2289 2262
Size: 27 6115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4267 5132 5430 4267
Size: 865 298 4979
Fragments arranged by size:
4979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2654 4805 882
Size: 617 1155 2151 2219
Fragments arranged by size:
2219 2151 1155 617
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3949 4331 4966 275
Size: 53 83 186 936 2416 382 635 1451
Fragments arranged by size:
2416 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 2862 2892 1178
Size: 30 165 30 984 30 445 30 4428
Fragments arranged by size:
4428 984 445 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6142
BseYI C'CCAG_C
Cuts at: 5345 5841 5345
Size: 496 5646
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3311 1163
Size: 573 1575 3994
Fragments arranged by size:
3994 1575 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 2170 3592 4353 5381 1540
Size: 275 186 129 40 1422 761 1028 2301
Fragments arranged by size:
2301 1422 1028 761 275 186 129 40
BsiHKAI G_wGCw'C
Cuts at: 4203 4288 5359 4203
Size: 85 1071 4986
Fragments arranged by size:
4986 1071 85
BsmI GAATG_Cn'
Cuts at: 3653 3746 3653
Size: 93 6049
BsmBI CGTCTCn'nnnn_
Cuts at: 2006 3353 2006
Size: 1347 4795
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2044 2075 2100 2141 2156 2200
Size: 399 231 23 31 25 41 15 44
Cuts at: 2200 2405 2478 3461 4203 4288 5359 1391
Size: 205 73 983 742 85 1071 2174
Fragments arranged by size:
2174 1071 983 742 399 231 205 85
73 44 41 31 25 23 15
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 3497 844
Size: 1239 1414 3489
Fragments arranged by size:
3489 1414 1239
BsrBI CCG'CTC
Cuts at: 1394 1823 2038 2176 2261 2288 2305 2890 4029
Size: 429 215 138 85 27 17 585 1139
Cuts at: 4029 1394
Size: 3507
Fragments arranged by size:
3507 1139 585 429 215 138 85 27
17
BsrDI GCAATG_nn'
Cuts at: 65 1248 2445 2941 4637 4811 65
Size: 1183 1197 496 1696 174 1396
Fragments arranged by size:
1696 1396 1197 1183 496 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 2865 4786 4973 1700
Size: 110 319 736 1921 187 2869
Fragments arranged by size:
2869 1921 736 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2875 794
Size: 2081 4061
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2599
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2323 3148 3226 3282 3472 3839 4224 4241
Size: 351 825 78 56 190 367 385 17
Cuts at: 4241 5693 5779 5791 6137 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 825 385 367 351 346 190
86 78 56 17 12
BstZ17I GTA'TAC
Cuts at: 2647 2647
Size: 6142
Bsu36I CC'TnA_GG
Cuts at: 2433 2433
Size: 6142
BtgI C'CryG_G
Cuts at: 513 1065 2004 2232 2268 513
Size: 552 939 228 36 4387
Fragments arranged by size:
4387 939 552 228 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 3658 4452 4472 3658
Size: 794 20 5328
Fragments arranged by size:
5328 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2555 5149 809
Size: 507 1239 2594 1802
Fragments arranged by size:
2594 1802 1239 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2256 2361
Size: 54 1425 50 275 18 297 129 105
Cuts at: 2361 2542 2652 3589 4478 6107 8
Size: 181 110 937 889 1629 43
Fragments arranged by size:
1629 1425 937 889 297 275 181 129
110 105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2127 3589 1537
Size: 275 315 1462 4090
Fragments arranged by size:
4090 1462 315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 2853 3192 3221 4072 1353
Size: 1113 387 339 29 851 3423
Fragments arranged by size:
3423 1113 851 387 339 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2136 4729 5103 5249 387
Size: 1064 138 547 2593 374 146 1280
Fragments arranged by size:
2593 1280 1064 547 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2389 2864 3083 3100 4205 4802 5589
Size: 393 757 475 219 17 1105 597 787
Cuts at: 5589 5857 1239
Size: 268 1524
Fragments arranged by size:
1524 1105 787 757 597 475 393 268
219 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2564 2564
Size: 6142
EcoO109I rG'GnC_Cy
Cuts at: 2138 2428 3202 2138
Size: 290 774 5078
Fragments arranged by size:
5078 774 290
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
EcoRV GAT'ATC
Cuts at: 3168 3168
Size: 6142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3445 3445
Size: 6142
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 2082 2374 5289 1678
Size: 404 292 2915 2531
Fragments arranged by size:
2915 2531 404 292
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3073 3105 3133 3165 3274
Size: 32 135 32 1501 32 28 32 109
Cuts at: 3274 3306 4783 4815 4857 4889 1373
Size: 32 1477 32 42 32 2626
Fragments arranged by size:
2626 1501 1477 135 109 42 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 2716 5256 5440 1305
Size: 864 547 2540 184 2007
Fragments arranged by size:
2540 2007 864 547 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 2654 10
Size: 54 1425 343 531 291 3498
Fragments arranged by size:
3498 1425 531 343 291 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4100 4459 4618 518
Size: 648 2934 359 159 2042
Fragments arranged by size:
2934 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2270 2409 2743 3379 3558 4235 5383 5628
Size: 664 139 334 636 179 677 1148 245
Cuts at: 5628 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 664 636 334 245 179
139
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 6142
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4651
Fragments arranged by size:
4651 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3500 5045 1132
Size: 97 2271 1545 2229
Fragments arranged by size:
2271 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2215 1348
Size: 867 5275
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2233 2254 2611 2735 2774 1108
Size: 1125 21 357 124 39 4476
Fragments arranged by size:
4476 1125 357 124 39 21
PfoI T'CCnGG_A
Cuts at: 2316 3077 2316
Size: 761 5381
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2428 3202 2428
Size: 774 5368
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PspOMI G'GGCC_C
Cuts at: 2040 2137 2196 2040
Size: 97 59 5986
Fragments arranged by size:
5986 97 59
PstI C_TGCA'G
Cuts at: 830 2517 3494 830
Size: 1687 977 3478
Fragments arranged by size:
3478 1687 977
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
RsrII CG'GwC_CG
Cuts at: 2110 2110
Size: 6142
SacII CC_GC'GG
Cuts at: 2271 2271
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SapI GCTCTTCn'nnn_
Cuts at: 2466 2466
Size: 6142
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2513 2671 3490 4625 5306 5497 826
Size: 219 1468 158 819 1135 681 191 1471
Fragments arranged by size:
1471 1468 1135 819 681 219 191 158
SfiI GGCCn_nnn'nGGCC
Cuts at: 2264 2360 2264
Size: 96 6046
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 2101 2201 3469 1872
Size: 229 100 1268 4545
Fragments arranged by size:
4545 1268 229 100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4246 5147 5409 820
Size: 197 926 2303 901 262 1553
Fragments arranged by size:
2303 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 2023 152
Size: 1871 4271
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 3302 1517
Size: 1785 4357
StyI C'CwwG_G
Cuts at: 513 1065 2004 3581 513
Size: 552 939 1577 3074
Fragments arranged by size:
3074 1577 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2700 2989 4169 4186 4339 4524 4969
Size: 1255 1120 289 1180 17 153 185 445
Cuts at: 4969 325
Size: 1498
Fragments arranged by size:
1498 1255 1180 1120 445 289 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4388 6100 96
Size: 274 80 33 51 1443 2411 1712 138
Fragments arranged by size:
2411 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6102 6134 6102
Size: 32 6110
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 2099 2199 3467 1870
Size: 229 100 1268 4545
Fragments arranged by size:
4545 1268 229 100
XmnI GAAnn'nnTTC
Cuts at: 2312 2847 3425 4271 2312
Size: 535 578 846 4183
Fragments arranged by size:
4183 846 578 535
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI
DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI PvuII SacI SanDI SexAI
SfoI SrfI SwaI XcmI