Restriction map for
pFN21AE3605
(Circular) (Six-base) MAPSORT of: pFN21AE3605.seq Check: 2429 from: 1 to: 6079
pFN21AE3605 6079 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2898 2898
Size: 6079
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3238 3400 3238
Size: 162 5917
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2260 2577 4142 5526 5794 1632
Size: 452 176 317 1565 1384 268 1917
Fragments arranged by size:
1917 1565 1384 452 317 268 176
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 2027 3123 4978 5955 1203
Size: 824 1096 1855 977 1327
Fragments arranged by size:
1855 1327 1096 977 824
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2160 2192 2160
Size: 32 6047
AlwNI CAG_nnn'CTG
Cuts at: 3082 3822 5394 3082
Size: 740 1572 3767
Fragments arranged by size:
3767 1572 740
ApaLI G'TGCA_C
Cuts at: 3275 4136 5292 3275
Size: 861 1156 4062
Fragments arranged by size:
4062 1156 861
ApoI r'AATT_y
Cuts at: 1493 2355 2621 3388 3614 3766 5875 1493
Size: 862 266 767 226 152 2109 1697
Fragments arranged by size:
2109 1697 862 767 266 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3143 3404 1870
Size: 73 1200 261 4545
Fragments arranged by size:
4545 1200 261 73
AvrII C'CTAG_G
Cuts at: 2054 2054
Size: 6079
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3238 3400 4855 618
Size: 325 217 627 1451 162 1455 1842
Fragments arranged by size:
1842 1455 1451 627 325 217 162
BanII G_rGCy'C
Cuts at: 1391 2021 3187 3398 1391
Size: 630 1166 211 4072
Fragments arranged by size:
4072 1166 630 211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 1903
Size: 34 2333 34 3678
Fragments arranged by size:
3678 2333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3971 5181 2277
Size: 1694 1210 3175
Fragments arranged by size:
3175 1694 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2323 4690 136
Size: 107 122 71 631 605 651 2367 1525
Fragments arranged by size:
2367 1525 651 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2901 6074 2901
Size: 3173 2906
BlpI GC'TnA_GC
Cuts at: 3310 3496 3310
Size: 186 5893
Bme1580I G_kGCm'C
Cuts at: 1790 3279 4140 5296 1790
Size: 1489 861 1156 2573
Fragments arranged by size:
2573 1489 1156 861
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3810 4768 442
Size: 416 53 83 399 24 2393 958 1753
Fragments arranged by size:
2393 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2422 2428 2828 2948 3017 3225 3369 4739
Size: 1183 6 400 120 69 208 144 1370
Cuts at: 4739 1239
Size: 2579
Fragments arranged by size:
2579 1370 1183 400 208 144 120 69
6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2949 3171 3392 4204 5069 5367 2949
Size: 222 221 812 865 298 3661
Fragments arranged by size:
3661 865 812 298 222 221
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4742 882
Size: 617 3243 2219
Fragments arranged by size:
3243 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3870 493
Size: 711 2666 2702
Fragments arranged by size:
2702 2666 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3886 4268 4903 275
Size: 53 83 186 936 2353 382 635 1451
Fragments arranged by size:
2353 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2269 3316 4512 4910 5184 5331 5742 2269
Size: 1047 1196 398 274 147 411 2606
Fragments arranged by size:
2606 1196 1047 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5854
Fragments arranged by size:
5854 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2577 2825 2912 3310 1186
Size: 1391 248 87 398 3955
Fragments arranged by size:
3955 1391 398 248 87
BseYI C'CCAG_C
Cuts at: 2488 3082 3257 5282 5778 2488
Size: 594 175 2025 496 2789
Fragments arranged by size:
2789 2025 594 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2837 3154 1163
Size: 573 1101 317 4088
Fragments arranged by size:
4088 1101 573 317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3529 4290 5318 1540
Size: 275 186 1528 761 1028 2301
Fragments arranged by size:
2301 1528 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2125 2976 3033 3279 4140 4225 5296 2125
Size: 851 57 246 861 85 1071 2908
Fragments arranged by size:
2908 1071 861 851 246 85 57
BsiWI C'GTAC_G
Cuts at: 2232 2232
Size: 6079
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2125 2976 3033 3187 3279 3398
Size: 399 231 104 851 57 154 92 119
Cuts at: 3398 4140 4225 5296 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 851 742 399 231 154 119
104 92 85 57
BspEI T'CCGG_A
Cuts at: 2269 5742 2269
Size: 3473 2606
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2898 3250 3434 844
Size: 2054 352 184 3489
Fragments arranged by size:
3489 2054 352 184
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2792 4574 4748 65
Size: 1183 1544 1782 174 1396
Fragments arranged by size:
1782 1544 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4723 4910 1700
Size: 110 2913 187 2869
Fragments arranged by size:
2913 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3009 3224 96
Size: 2913 215 2951
Fragments arranged by size:
2951 2913 215
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstXI CCAn_nnnn'nTGG
Cuts at: 2452 2452
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2096 2901 3409 3776 4161 4178 5630 5716
Size: 124 805 508 367 385 17 1452 86
Cuts at: 5716 5728 6074 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 805 508 385 367 346 124
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2895 2895
Size: 6079
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 2673 3595 4389 4409 2673
Size: 922 794 20 4343
Fragments arranged by size:
4343 922 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2247 3526 4415
Size: 54 1425 50 275 18 417 1279 889
Cuts at: 4415 6044 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1279 889 417 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3526 1537
Size: 275 1714 4090
Fragments arranged by size:
4090 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2125 4009 1353
Size: 772 1884 3423
Fragments arranged by size:
3423 1884 772
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2260 2422 2428 2577 2828 2948
Size: 393 452 176 162 6 149 251 120
Cuts at: 2948 3017 3225 3369 4142 4739 5526 5794 1239
Size: 69 208 144 773 597 787 268 1524
Fragments arranged by size:
1524 787 773 597 452 393 268 251
208 176 162 149 144 120 69 6
EcoRI G'AATT_C
Cuts at: 2355 3388 3766 2355
Size: 1033 378 4668
Fragments arranged by size:
4668 1033 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2888 2888
Size: 6079
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 5226 1678
Size: 3548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2728 2760 4720 4752 4794
Size: 32 135 32 1156 32 1960 32 42
Cuts at: 4794 4826 1373
Size: 32 2626
Fragments arranged by size:
2626 1960 1156 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 3423 3669 669
Size: 988 281 199 1286 246 3079
Fragments arranged by size:
3079 1286 988 281 246 199
HindIII A'AGCT_T
Cuts at: 748 2779 3282 3307 748
Size: 2031 503 25 3520
Fragments arranged by size:
3520 2031 503 25
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3242 3404 3242
Size: 162 5917
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 2027 5955 2027
Size: 3928 2151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2266 3152 5193 5377 1305
Size: 961 886 2041 184 2007
Fragments arranged by size:
2041 2007 961 886 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 10
Size: 54 1425 343 417 3840
Fragments arranged by size:
3840 1425 417 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4037 4396 4555 518
Size: 648 2871 359 159 2042
Fragments arranged by size:
2871 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3079 3138 3157 3495 4172 5320 5565 1606
Size: 1473 59 19 338 677 1148 245 2120
Fragments arranged by size:
2120 1473 1148 677 338 245 59 19
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 3008 3127 3437 4982 1132
Size: 97 1779 119 310 1545 2229
Fragments arranged by size:
2229 1779 1545 310 119 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 3123 4978 3123
Size: 1855 4224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2429 3082 1108
Size: 1321 653 4105
Fragments arranged by size:
4105 1321 653
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3168 1613
Size: 1555 4524
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PstI C_TGCA'G
Cuts at: 830 2909 3431 830
Size: 2079 522 3478
Fragments arranged by size:
3478 2079 522
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 3079 3138 3157 3079
Size: 59 19 6001
Fragments arranged by size:
6001 59 19
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 2281 2799 4327 6039 2281
Size: 518 1528 1712 2321
Fragments arranged by size:
2321 1712 1528 518
SfcI C'TryA_G
Cuts at: 826 1045 2010 2190 2214 2905 3427 4562 5243
Size: 219 965 180 24 691 522 1135 681
Cuts at: 5243 5434 826
Size: 191 1471
Fragments arranged by size:
1471 1135 965 691 681 522 219 191
180 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2964 3186 3371 4183 5084 5346
Size: 197 926 1021 222 185 812 901 262
Cuts at: 5346 820
Size: 1553
Fragments arranged by size:
1553 1021 926 901 812 262 222 197
185
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 2282 152
Size: 2130 3949
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2004 2054 2505 3518 513
Size: 552 939 50 451 1013 3074
Fragments arranged by size:
3074 1013 939 552 451 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2279 2797 3009
Size: 274 80 33 51 1443 302 518 212
Cuts at: 3009 3224 4325 6037 96
Size: 215 1101 1712 138
Fragments arranged by size:
1712 1443 1101 518 302 274 215 212
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6039 6071 6039
Size: 32 6047
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 2839 3415 2839
Size: 576 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2689 3299 2689
Size: 610 5469
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI