(Circular) (Six-base) MAPSORT of: pFN21AE3605.seq Check: 2429 from: 1 to: 6079 pFN21AE3605 6079 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2898 2898 Size: 6079 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3238 3400 3238 Size: 162 5917 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2260 2577 4142 5526 5794 1632 Size: 452 176 317 1565 1384 268 1917 Fragments arranged by size: 1917 1565 1384 452 317 268 176 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 2027 3123 4978 5955 1203 Size: 824 1096 1855 977 1327 Fragments arranged by size: 1855 1327 1096 977 824 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2160 2192 2160 Size: 32 6047 AlwNI CAG_nnn'CTG Cuts at: 3082 3822 5394 3082 Size: 740 1572 3767 Fragments arranged by size: 3767 1572 740 ApaLI G'TGCA_C Cuts at: 3275 4136 5292 3275 Size: 861 1156 4062 Fragments arranged by size: 4062 1156 861 ApoI r'AATT_y Cuts at: 1493 2355 2621 3388 3614 3766 5875 1493 Size: 862 266 767 226 152 2109 1697 Fragments arranged by size: 2109 1697 862 767 266 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3143 3404 1870 Size: 73 1200 261 4545 Fragments arranged by size: 4545 1200 261 73 AvrII C'CTAG_G Cuts at: 2054 2054 Size: 6079 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3238 3400 4855 618 Size: 325 217 627 1451 162 1455 1842 Fragments arranged by size: 1842 1455 1451 627 325 217 162 BanII G_rGCy'C Cuts at: 1391 2021 3187 3398 1391 Size: 630 1166 211 4072 Fragments arranged by size: 4072 1166 630 211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4270 4304 1903 Size: 34 2333 34 3678 Fragments arranged by size: 3678 2333 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2277 3971 5181 2277 Size: 1694 1210 3175 Fragments arranged by size: 3175 1694 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2323 4690 136 Size: 107 122 71 631 605 651 2367 1525 Fragments arranged by size: 2367 1525 651 631 605 122 107 71 BglII A'GATC_T Cuts at: 2901 6074 2901 Size: 3173 2906 BlpI GC'TnA_GC Cuts at: 3310 3496 3310 Size: 186 5893 Bme1580I G_kGCm'C Cuts at: 1790 3279 4140 5296 1790 Size: 1489 861 1156 2573 Fragments arranged by size: 2573 1489 1156 861 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3810 4768 442 Size: 416 53 83 399 24 2393 958 1753 Fragments arranged by size: 2393 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2422 2428 2828 2948 3017 3225 3369 4739 Size: 1183 6 400 120 69 208 144 1370 Cuts at: 4739 1239 Size: 2579 Fragments arranged by size: 2579 1370 1183 400 208 144 120 69 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2949 3171 3392 4204 5069 5367 2949 Size: 222 221 812 865 298 3661 Fragments arranged by size: 3661 865 812 298 222 221 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4742 882 Size: 617 3243 2219 Fragments arranged by size: 3243 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3870 493 Size: 711 2666 2702 Fragments arranged by size: 2702 2666 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3886 4268 4903 275 Size: 53 83 186 936 2353 382 635 1451 Fragments arranged by size: 2353 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2269 3316 4512 4910 5184 5331 5742 2269 Size: 1047 1196 398 274 147 411 2606 Fragments arranged by size: 2606 1196 1047 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5854 Fragments arranged by size: 5854 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2577 2825 2912 3310 1186 Size: 1391 248 87 398 3955 Fragments arranged by size: 3955 1391 398 248 87 BseYI C'CCAG_C Cuts at: 2488 3082 3257 5282 5778 2488 Size: 594 175 2025 496 2789 Fragments arranged by size: 2789 2025 594 496 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2837 3154 1163 Size: 573 1101 317 4088 Fragments arranged by size: 4088 1101 573 317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3529 4290 5318 1540 Size: 275 186 1528 761 1028 2301 Fragments arranged by size: 2301 1528 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2125 2976 3033 3279 4140 4225 5296 2125 Size: 851 57 246 861 85 1071 2908 Fragments arranged by size: 2908 1071 861 851 246 85 57 BsiWI C'GTAC_G Cuts at: 2232 2232 Size: 6079 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2125 2976 3033 3187 3279 3398 Size: 399 231 104 851 57 154 92 119 Cuts at: 3398 4140 4225 5296 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 851 742 399 231 154 119 104 92 85 57 BspEI T'CCGG_A Cuts at: 2269 5742 2269 Size: 3473 2606 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2898 3250 3434 844 Size: 2054 352 184 3489 Fragments arranged by size: 3489 2054 352 184 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2792 4574 4748 65 Size: 1183 1544 1782 174 1396 Fragments arranged by size: 1782 1544 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4723 4910 1700 Size: 110 2913 187 2869 Fragments arranged by size: 2913 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3009 3224 96 Size: 2913 215 2951 Fragments arranged by size: 2951 2913 215 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstXI CCAn_nnnn'nTGG Cuts at: 2452 2452 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2096 2901 3409 3776 4161 4178 5630 5716 Size: 124 805 508 367 385 17 1452 86 Cuts at: 5716 5728 6074 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 805 508 385 367 346 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2895 2895 Size: 6079 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 2673 3595 4389 4409 2673 Size: 922 794 20 4343 Fragments arranged by size: 4343 922 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 3526 4415 Size: 54 1425 50 275 18 417 1279 889 Cuts at: 4415 6044 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1279 889 417 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3526 1537 Size: 275 1714 4090 Fragments arranged by size: 4090 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2125 4009 1353 Size: 772 1884 3423 Fragments arranged by size: 3423 1884 772 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2260 2422 2428 2577 2828 2948 Size: 393 452 176 162 6 149 251 120 Cuts at: 2948 3017 3225 3369 4142 4739 5526 5794 1239 Size: 69 208 144 773 597 787 268 1524 Fragments arranged by size: 1524 787 773 597 452 393 268 251 208 176 162 149 144 120 69 6 EcoRI G'AATT_C Cuts at: 2355 3388 3766 2355 Size: 1033 378 4668 Fragments arranged by size: 4668 1033 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2888 2888 Size: 6079 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 5226 1678 Size: 3548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2728 2760 4720 4752 4794 Size: 32 135 32 1156 32 1960 32 42 Cuts at: 4794 4826 1373 Size: 32 2626 Fragments arranged by size: 2626 1960 1156 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2137 3423 3669 669 Size: 988 281 199 1286 246 3079 Fragments arranged by size: 3079 1286 988 281 246 199 HindIII A'AGCT_T Cuts at: 748 2779 3282 3307 748 Size: 2031 503 25 3520 Fragments arranged by size: 3520 2031 503 25 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3242 3404 3242 Size: 162 5917 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 2027 5955 2027 Size: 3928 2151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2266 3152 5193 5377 1305 Size: 961 886 2041 184 2007 Fragments arranged by size: 2041 2007 961 886 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 10 Size: 54 1425 343 417 3840 Fragments arranged by size: 3840 1425 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4037 4396 4555 518 Size: 648 2871 359 159 2042 Fragments arranged by size: 2871 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3079 3138 3157 3495 4172 5320 5565 1606 Size: 1473 59 19 338 677 1148 245 2120 Fragments arranged by size: 2120 1473 1148 677 338 245 59 19 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 3008 3127 3437 4982 1132 Size: 97 1779 119 310 1545 2229 Fragments arranged by size: 2229 1779 1545 310 119 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 3123 4978 3123 Size: 1855 4224 PflMI CCAn_nnn'nTGG Cuts at: 1108 2429 3082 1108 Size: 1321 653 4105 Fragments arranged by size: 4105 1321 653 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3168 1613 Size: 1555 4524 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PstI C_TGCA'G Cuts at: 830 2909 3431 830 Size: 2079 522 3478 Fragments arranged by size: 3478 2079 522 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 3079 3138 3157 3079 Size: 59 19 6001 Fragments arranged by size: 6001 59 19 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 2281 2799 4327 6039 2281 Size: 518 1528 1712 2321 Fragments arranged by size: 2321 1712 1528 518 SfcI C'TryA_G Cuts at: 826 1045 2010 2190 2214 2905 3427 4562 5243 Size: 219 965 180 24 691 522 1135 681 Cuts at: 5243 5434 826 Size: 191 1471 Fragments arranged by size: 1471 1135 965 691 681 522 219 191 180 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2964 3186 3371 4183 5084 5346 Size: 197 926 1021 222 185 812 901 262 Cuts at: 5346 820 Size: 1553 Fragments arranged by size: 1553 1021 926 901 812 262 222 197 185 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 2282 152 Size: 2130 3949 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2004 2054 2505 3518 513 Size: 552 939 50 451 1013 3074 Fragments arranged by size: 3074 1013 939 552 451 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2279 2797 3009 Size: 274 80 33 51 1443 302 518 212 Cuts at: 3009 3224 4325 6037 96 Size: 215 1101 1712 138 Fragments arranged by size: 1712 1443 1101 518 302 274 215 212 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6039 6071 6039 Size: 32 6047 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 2839 3415 2839 Size: 576 5503 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2689 3299 2689 Size: 610 5469 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI