Restriction map for
pFN21AE3604
(Circular) (Six-base) MAPSORT of: pFN21AE3604.seq Check: 3320 from: 1 to: 5767
pFN21AE3604 5767 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2736 3110 1937
Size: 799 374 4594
Fragments arranged by size:
4594 799 374
Acc65I G'GTAC_C
Cuts at: 2148 3088 2148
Size: 940 4827
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2019 2028 2825 3830 5214 5482 1632
Size: 387 9 797 1005 1384 268 1917
Fragments arranged by size:
1917 1384 1005 797 387 268 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 2189 4666 5643 1203
Size: 986 2477 977 1327
Fragments arranged by size:
2477 1327 986 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 2602 1166
Size: 1436 4331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 2583 3510 5082 2583
Size: 927 1572 3268
Fragments arranged by size:
3268 1572 927
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 2023 2535 2660 3076 3302 3454 5563 1493
Size: 530 512 125 416 226 152 2109 1697
Fragments arranged by size:
2109 1697 530 512 416 226 152 125
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 1870
Size: 73 1149 4545
Fragments arranged by size:
4545 1149 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2148 3088 4543 618
Size: 325 217 627 361 940 1455 1842
Fragments arranged by size:
1842 1455 940 627 361 325 217
BanII G_rGCy'C
Cuts at: 1391 3086 1391
Size: 1695 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2754 928
Size: 971 855 3941
Fragments arranged by size:
3941 971 855
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2141 2160 4378
Size: 107 122 71 631 605 469 19 2218
Cuts at: 4378 136
Size: 1525
Fragments arranged by size:
2218 1525 631 605 469 122 107 71
19
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 2189 3008 3828 4984 1790
Size: 399 819 820 1156 2573
Fragments arranged by size:
2573 1156 820 819 399
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2089 3498 4456
Size: 416 53 83 399 24 672 1409 958
Cuts at: 4456 442
Size: 1753
Fragments arranged by size:
1753 1409 958 672 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2921 3008 3020 3084 4427 1239
Size: 1214 468 87 12 64 1343 2579
Fragments arranged by size:
2579 1343 1214 468 87 64 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2924 1186
Size: 1738 4029
BseYI C'CCAG_C
Cuts at: 2312 2904 2910 3048 4970 5466 2312
Size: 592 6 138 1922 496 2613
Fragments arranged by size:
2613 1922 592 496 138 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2569 1163
Size: 573 833 4361
Fragments arranged by size:
4361 833 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2736 3217 3978 5006 1540
Size: 275 186 735 481 761 1028 2301
Fragments arranged by size:
2301 1028 761 735 481 275 186
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
BsmBI CGTCTCn'nnnn_
Cuts at: 2889 2889
Size: 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 3008 3086 3828 3913 4984 1391
Size: 399 399 819 78 742 85 1071 2174
Fragments arranged by size:
2174 1071 819 742 399 399 85 78
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 2383 96
Size: 2287 3480
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2770 3097 3464 3849 3866 5318 5404 5416
Size: 798 327 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 798 385 367 346 327 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2225 487
Size: 683 56 999 4029
Fragments arranged by size:
4029 999 683 56
BtsI GCAGTG_nn'
Cuts at: 2597 2609 2637 3283 4077 4097 2597
Size: 12 28 646 794 20 4267
Fragments arranged by size:
4267 794 646 28 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2392 3214 4103
Size: 54 1425 50 275 18 562 822 889
Cuts at: 4103 5732 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 822 562 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2844 3697 1353
Size: 1491 853 3423
Fragments arranged by size:
3423 1491 853
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2019 2028 2453 2825 2921 3008 3020
Size: 393 387 9 425 372 96 87 12
Cuts at: 3020 3084 3830 4427 5214 5482 1239
Size: 64 746 597 787 268 1524
Fragments arranged by size:
1524 787 746 597 425 393 387 372
268 96 87 64 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2936 2936
Size: 5767
EcoO109I rG'GnC_Cy
Cuts at: 2920 2920
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
EcoRV GAT'ATC
Cuts at: 2350 2350
Size: 5767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 5767
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2166 4914 1678
Size: 488 2748 2531
Fragments arranged by size:
2748 2531 488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2788 2794 2820 4408
Size: 32 135 32 1190 26 6 26 1588
Cuts at: 4408 4440 4482 4514 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1588 1190 135 42 32 32 32
32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2737 3111 3357 669
Size: 988 281 799 374 246 3079
Fragments arranged by size:
3079 988 799 374 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 2152 3092 2152
Size: 940 4827
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2394 10
Size: 54 1425 343 562 3383
Fragments arranged by size:
3383 1425 562 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2602 3725 4084 4243 518
Size: 648 1436 1123 359 159 2042
Fragments arranged by size:
2042 1436 1123 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2821 3183 3860 5008 5253 1606
Size: 1215 362 677 1148 245 2120
Fragments arranged by size:
2120 1215 1148 677 362 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5215
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 3033 3125 4670 1132
Size: 97 1804 92 1545 2229
Fragments arranged by size:
2229 1804 1545 97 92
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2767 1108
Size: 1659 4108
PfoI T'CCnGG_A
Cuts at: 2766 2766
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2920 2920
Size: 5767
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2451 2604 3119 830
Size: 1621 153 515 3478
Fragments arranged by size:
3478 1621 515 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2821 2821
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 2735 3109 1936
Size: 799 374 4594
Fragments arranged by size:
4594 799 374
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2447 2600 3115 4250 4931 5122 826
Size: 219 1402 153 515 1135 681 191 1471
Fragments arranged by size:
1471 1402 1135 681 515 219 191 153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3033 3125 1132
Size: 1901 92 3774
Fragments arranged by size:
3774 1901 92
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 3206 513
Size: 552 2141 3074
Fragments arranged by size:
3074 2141 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3794 3811 3964 4149 4594 325
Size: 1255 2214 17 153 185 445 1498
Fragments arranged by size:
2214 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2383 2866 4013
Size: 274 80 33 51 1443 406 483 1147
Cuts at: 4013 5725 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1147 483 406 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2076 2076
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI