Restriction map for
pFN21AE3603
(Circular) (Six-base) MAPSORT of: pFN21AE3603.seq Check: 7855 from: 1 to: 5338
pFN21AE3603 5338 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2580 3148 278
Size: 53 83 186 936 1044 568 2468
Fragments arranged by size:
2468 1044 936 568 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AfeI AGC'GCT
Cuts at: 2087 2087
Size: 5338
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 2359 4169 2359
Size: 1810 3528
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 3081 4653 3081
Size: 1572 3766
ApaLI G'TGCA_C
Cuts at: 2093 3395 4551 2093
Size: 1302 1156 2880
Fragments arranged by size:
2880 1302 1156
ApoI r'AATT_y
Cuts at: 1493 2647 2873 3025 5134 1493
Size: 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 1870
Size: 73 720 4545
Fragments arranged by size:
4545 720 73
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2659 4114 618
Size: 325 217 627 872 1455 1842
Fragments arranged by size:
1842 1455 872 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2657 1391
Size: 1266 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2075 2263 928
Size: 971 176 188 4003
Fragments arranged by size:
4003 971 188 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3529 3563 1903
Size: 34 50 34 1508 34 3678
Fragments arranged by size:
3678 1508 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3949 136
Size: 107 122 71 631 605 337 1940 1525
Fragments arranged by size:
1940 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2097 3399 4555 1790
Size: 307 1302 1156 2573
Fragments arranged by size:
2573 1302 1156 307
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2170 3069 4027
Size: 416 53 83 399 24 753 899 958
Cuts at: 4027 442
Size: 1753
Fragments arranged by size:
1753 958 899 753 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2497 3998 1239
Size: 1258 1501 2579
Fragments arranged by size:
2579 1501 1258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 2411 3463 4328 4626 2132
Size: 279 1052 865 298 2844
Fragments arranged by size:
2844 1052 865 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2092 3129 493
Size: 711 888 1037 2702
Fragments arranged by size:
2702 1037 888 711
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2577 3145 3527 4162
Size: 53 83 186 936 1044 568 382 635
Cuts at: 4162 275
Size: 1451
Fragments arranged by size:
1451 1044 936 635 568 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2359 3771 4169 4443 4590 5001 2359
Size: 1412 398 274 147 411 2696
Fragments arranged by size:
2696 1412 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 1178
Size: 30 165 30 612 30 4471
Fragments arranged by size:
4471 612 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 1186
Size: 912 4426
BseYI C'CCAG_C
Cuts at: 4541 5037 4541
Size: 496 4842
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4765
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2788 3549 4577 1540
Size: 275 186 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2097 2157 2412 2454 3399 3484 4555 2028
Size: 69 60 255 42 945 85 1071 2811
Fragments arranged by size:
2811 1071 945 255 85 69 60 42
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2097 2157 2412 2454 2657 3399
Size: 399 238 69 60 255 42 203 742
Cuts at: 3399 3484 4555 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 255 238 203 85
69 60 42
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 2412 3227 4957 2412
Size: 815 1730 2793
Fragments arranged by size:
2793 1730 815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2195 2693 844
Size: 1351 498 3489
Fragments arranged by size:
3489 1351 498
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 1394
Size: 429 1402 3507
Fragments arranged by size:
3507 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2359 3982 4169 1700
Size: 110 549 1623 187 2869
Fragments arranged by size:
2869 1623 549 187 110
BsrGI T'GTAC_A
Cuts at: 96 2031 96
Size: 1935 3403
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstXI CCAn_nnnn'nTGG
Cuts at: 2521 2521
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333
Size: 696 367 385 17 1452 86 12 346
Cuts at: 5333 1972
Size: 1977
Fragments arranged by size:
1977 1452 696 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4786
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2310 2854 3648 3668 2310
Size: 544 794 20 3980
Fragments arranged by size:
3980 794 544 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2115 2150 529
Size: 35 1551 35 3717
Fragments arranged by size:
3717 1551 35 35
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 2785 3674
Size: 54 1425 50 275 18 549 406 889
Cuts at: 3674 5303 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 549 406 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2785 1537
Size: 275 973 4090
Fragments arranged by size:
4090 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3268 1353
Size: 1915 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3925 4299 4445 387
Size: 1064 138 2336 374 146 1280
Fragments arranged by size:
2336 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2497 3401 3998 4785 5053 1239
Size: 393 865 904 597 787 268 1524
Fragments arranged by size:
1524 904 865 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2011 2164 2011
Size: 153 5185
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
EcoRV GAT'ATC
Cuts at: 2200 2200
Size: 5338
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2089 4485 1678
Size: 411 2396 2531
Fragments arranged by size:
2531 2396 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2495 2527 3979
Size: 32 135 32 743 32 148 32 1452
Cuts at: 3979 4011 4053 4085 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1452 743 148 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4452 4636 1305
Size: 3147 184 2007
Fragments arranged by size:
3147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 10
Size: 54 1425 343 549 2967
Fragments arranged by size:
2967 1425 549 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3296 3655 3814 518
Size: 648 2130 359 159 2042
Fragments arranged by size:
2130 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2616 2754 3431 4579 4824 1606
Size: 1010 138 677 1148 245 2120
Fragments arranged by size:
2120 1148 1010 677 245 138
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4786
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2125 3407 3439 4920 4952 2093
Size: 32 1282 32 1481 32 2479
Fragments arranged by size:
2479 1481 1282 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2164
Size: 5338
PshAI GACnn'nnGTC
Cuts at: 1613 2234 1613
Size: 621 4717
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2190 2690 830
Size: 1360 500 3478
Fragments arranged by size:
3478 1360 500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2616 2616
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SanDI GG'GwC_CC
Cuts at: 2164 2164
Size: 5338
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2186 2686 3821 4502 4693 826
Size: 219 1141 500 1135 681 191 1471
Fragments arranged by size:
1471 1141 1135 681 500 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 2426 3442 4343 4605 820
Size: 197 926 204 279 1016 901 262 1553
Fragments arranged by size:
1553 1016 926 901 279 262 204 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2604 2777 513
Size: 552 1539 173 3074
Fragments arranged by size:
3074 1539 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 3365 3382 3535 3720 4165 325
Size: 1255 666 1119 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1119 666 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 3584 5296
Size: 274 80 33 51 1443 54 1553 1712
Cuts at: 5296 96
Size: 138
Fragments arranged by size:
1712 1553 1443 274 138 80 54 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2131
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2578 3146 276
Size: 53 83 186 936 1044 568 2468
Fragments arranged by size:
2468 1044 936 568 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI