Restriction map for
pFN21AE3602
(Circular) (Six-base) MAPSORT of: pFN21AE3602.seq Check: 4938 from: 1 to: 6190
pFN21AE3602 6190 bp
With 182 enzymes: SgfI *
March 28, 2012 14:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2100 4000 278
Size: 53 83 186 936 564 1900 2468
Fragments arranged by size:
2468 1900 936 564 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2709 3533 1937
Size: 772 824 4594
Fragments arranged by size:
4594 824 772
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 2941 3370 4253 5637 5905 1632
Size: 668 641 429 883 1384 268 1917
Fragments arranged by size:
1917 1384 883 668 641 429 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 2154 5089 6066 1203
Size: 951 2935 977 1327
Fragments arranged by size:
2935 1327 977 951
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 2646 4919 2646
Size: 2273 3917
AleI CACnn'nnGTG
Cuts at: 1166 2822 3302 1166
Size: 1656 480 4054
Fragments arranged by size:
4054 1656 480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AlwNI CAG_nnn'CTG
Cuts at: 2226 2758 3020 3109 3226 3229 3393 3933 5505
Size: 532 262 89 117 3 164 540 1572
Cuts at: 5505 2226
Size: 2911
Fragments arranged by size:
2911 1572 540 532 262 164 117 89
3
ApaI G_GGCC'C
Cuts at: 3184 3184
Size: 6190
ApaLI G'TGCA_C
Cuts at: 2472 3461 4247 5403 2472
Size: 989 786 1156 3259
Fragments arranged by size:
3259 1156 989 786
ApoI r'AATT_y
Cuts at: 1493 3499 3725 3877 5986 1493
Size: 2006 226 152 2109 1697
Fragments arranged by size:
2109 2006 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 2485 2831 3162 3515 1870
Size: 73 542 346 331 353 4545
Fragments arranged by size:
4545 542 353 346 331 73
BamHI G'GATC_C
Cuts at: 3520 3887 3520
Size: 367 5823
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2615 3166 3511 4966 618
Size: 325 217 627 828 551 345 1455 1842
Fragments arranged by size:
1842 1455 828 627 551 345 325 217
BanII G_rGCy'C
Cuts at: 1391 2955 3151 3184 3509 1391
Size: 1564 196 33 325 4072
Fragments arranged by size:
4072 1564 325 196 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2995 928
Size: 971 1096 4123
Fragments arranged by size:
4123 1096 971
BbvCI CC'TCA_GC
Cuts at: 2655 2655
Size: 6190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2401 2435 3067 3101 4381 4415 1903
Size: 34 464 34 632 34 1280 34 3678
Fragments arranged by size:
3678 1280 632 464 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4082 5292 4082
Size: 1210 4980
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2064 3188 4801
Size: 107 122 71 631 605 392 1124 1613
Cuts at: 4801 136
Size: 1525
Fragments arranged by size:
1613 1525 1124 631 605 392 122 107
71
BglII A'GATC_T
Cuts at: 2938 6185 2938
Size: 3247 2943
BlpI GC'TnA_GC
Cuts at: 3150 3607 3150
Size: 457 5733
Bme1580I G_kGCm'C
Cuts at: 1790 2328 2348 2476 2773 3184 3380 3465 4251
Size: 538 20 128 297 411 196 85 786
Cuts at: 4251 5407 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 786 538 411 297 196 128
85 20
BmgBI CAC'GTC
Cuts at: 1318 2157 1318
Size: 839 5351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2443 2603 3008
Size: 416 53 83 399 24 1026 160 405
Cuts at: 3008 3921 4879 442
Size: 913 958 1753
Fragments arranged by size:
1753 1026 958 913 416 405 399 160
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2555 2735 2759 4850 1239
Size: 997 319 180 24 2091 2579
Fragments arranged by size:
2579 2091 997 319 180 24
Bpu10I CC'TnA_GC
Cuts at: 2655 2655
Size: 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 4315 5180 5478 2102
Size: 2213 865 298 2814
Fragments arranged by size:
2814 2213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4853 882
Size: 617 3354 2219
Fragments arranged by size:
3354 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 6184 6184
Size: 6190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 3997 4379 5014
Size: 53 83 186 936 564 1900 382 635
Cuts at: 5014 275
Size: 1451
Fragments arranged by size:
1900 1451 936 635 564 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4623 5021 5295 5442 5853 4623
Size: 398 274 147 411 4960
Fragments arranged by size:
4960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3374 3404 1178
Size: 30 165 30 1971 30 3964
Fragments arranged by size:
3964 1971 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2956 1186
Size: 1770 4420
BseYI C'CCAG_C
Cuts at: 2511 2556 2865 3020 3309 5393 5889 2511
Size: 45 309 155 289 2084 496 2812
Fragments arranged by size:
2812 2084 496 309 289 155 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3239 1163
Size: 573 1503 4114
Fragments arranged by size:
4114 1503 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2424 2476 3465 4251 4336 5407 2424
Size: 52 989 786 85 1071 3207
Fragments arranged by size:
3207 1071 989 786 85 52
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2348 2424 2476 2773 2955 3151
Size: 399 538 20 76 52 297 182 196
Cuts at: 3151 3184 3380 3465 3509 4251 4336 5407 1391
Size: 33 196 85 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 297 196 196
182 85 85 76 52 44 33 20
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 4079 5809 4079
Size: 1730 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2538 3545 844
Size: 1694 1007 3489
Fragments arranged by size:
3489 1694 1007
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4685 4859 65
Size: 1183 3437 174 1396
Fragments arranged by size:
3437 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2063 2179 2565 4834 5021 1700
Size: 110 253 116 386 2269 187 2869
Fragments arranged by size:
2869 2269 386 253 187 116 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 3256 1925
Size: 1331 4859
BssSI C'ACGA_G
Cuts at: 4250 5262 4250
Size: 1012 5178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3226 794
Size: 2432 3758
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstXI CCAn_nnnn'nTGG
Cuts at: 2608 2608
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2938 3520 3887 4272 4289 5741 5827 5839
Size: 966 582 367 385 17 1452 86 12
Cuts at: 5839 6185 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 966 582 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2579 2601 2782 2859 3090
Size: 552 939 48 527 22 181 77 231
Cuts at: 3090 3291 513
Size: 201 3412
Fragments arranged by size:
3412 939 552 527 231 201 181 77
48 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 2024 2436 2923 3129 3297 3309 3706 4500 4520
Size: 412 487 206 168 12 397 794 20
Cuts at: 4520 2024
Size: 3694
Fragments arranged by size:
3694 794 487 412 397 206 168 20
12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 3494 3494
Size: 6190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2046 2177
Size: 54 1425 50 275 18 177 39 131
Cuts at: 2177 2576 2692 2734 2763 2801 3093 3189 3637
Size: 399 116 42 29 38 292 96 448
Cuts at: 3637 4526 6155 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 448 399 292 275 177
131 116 96 54 50 43 42 39
38 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2490 2960 3419 4120 1353
Size: 1137 470 459 701 3423
Fragments arranged by size:
3423 1137 701 470 459
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3457 4777 5151 5297 387
Size: 1064 138 1868 1320 374 146 1280
Fragments arranged by size:
1868 1320 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2300 2555 2735 2759 2941 3370
Size: 393 604 64 255 180 24 182 429
Cuts at: 3370 4253 4850 5637 5905 1239
Size: 883 597 787 268 1524
Fragments arranged by size:
1524 883 787 604 597 429 393 268
255 182 180 64 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2306
Size: 6190
EcoO109I rG'GnC_Cy
Cuts at: 2746 2746
Size: 6190
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FspI TGC'GCA
Cuts at: 3371 4696 3371
Size: 1325 4865
HaeII r_GCGC'y
Cuts at: 1678 2315 3317 5337 1678
Size: 637 1002 2020 2531
Fragments arranged by size:
2531 2020 1002 637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2632 2664 3133 3165 3374
Size: 32 135 32 1060 32 469 32 209
Cuts at: 3374 3406 4831 4863 4905 4937 1373
Size: 32 1425 32 42 32 2626
Fragments arranged by size:
2626 1425 1060 469 209 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3780 669
Size: 988 281 1596 246 3079
Fragments arranged by size:
3079 1596 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2275 5304 5488 1305
Size: 970 3029 184 2007
Fragments arranged by size:
3029 2007 970 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2765 3095 10
Size: 54 1425 343 933 330 3105
Fragments arranged by size:
3105 1425 933 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2429 2822 3215 3302 4148 4507
Size: 648 993 270 393 393 87 846 359
Cuts at: 4507 4666 518
Size: 159 2042
Fragments arranged by size:
2042 993 846 648 393 393 359 270
159 87
MspA1I CmG'CkG
Cuts at: 1606 2038 2054 2372 2654 2680 2869 3109 3154
Size: 432 16 318 282 26 189 240 45
Cuts at: 3154 3226 3293 3606 4283 5431 5676 1606
Size: 72 67 313 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 432 318 313 282 245
240 189 72 67 45 26 16
NaeI GCC'GGC
Cuts at: 2065 2181 2065
Size: 116 6074
NcoI C'CATG_G
Cuts at: 513 1065 2004 2601 2859 513
Size: 552 939 597 258 3844
Fragments arranged by size:
3844 939 597 552 258
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NgoMIV G'CCGG_C
Cuts at: 2063 2179 2063
Size: 116 6074
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 3328 3548 5093 1132
Size: 97 2099 220 1545 2229
Fragments arranged by size:
2229 2099 1545 220 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 5089 5089
Size: 6190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3020 1108
Size: 1912 4278
PfoI T'CCnGG_A
Cuts at: 2100 2133 2100
Size: 33 6157
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2746 2746
Size: 6190
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PspOMI G'GGCC_C
Cuts at: 3180 3180
Size: 6190
PstI C_TGCA'G
Cuts at: 830 3105 3304 3542 830
Size: 2275 199 238 3478
Fragments arranged by size:
3478 2275 238 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 2654 2680 3109 3154 3226 2654
Size: 26 429 45 72 5618
Fragments arranged by size:
5618 429 72 45 26
SacII CC_GC'GG
Cuts at: 2055 3294 2055
Size: 1239 4951
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 2960 3419 2960
Size: 459 5731
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SexAI A'CCwGG_T
Cuts at: 2742 2742
Size: 6190
SfcI C'TryA_G
Cuts at: 826 1045 3101 3300 3538 4673 5354 5545 826
Size: 219 2056 199 238 1135 681 191 1471
Fragments arranged by size:
2056 1471 1135 681 238 219 199 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2064 3188 2064
Size: 1124 5066
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3517 1872
Size: 1645 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 4294 5195 5457 820
Size: 197 926 174 2177 901 262 1553
Fragments arranged by size:
2177 1553 926 901 262 197 174
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3548 1132
Size: 2416 3774
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 2408 1517
Size: 891 5299
StyI C'CwwG_G
Cuts at: 513 1065 2004 2601 2859 3629 513
Size: 552 939 597 258 770 3074
Fragments arranged by size:
3074 939 770 597 552 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2503 2548 2729 2834 4217 4234 4387
Size: 1255 923 45 181 105 1383 17 153
Cuts at: 4387 4572 5017 325
Size: 185 445 1498
Fragments arranged by size:
1498 1383 1255 923 445 185 181 153
105 45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4436 6148 96
Size: 274 80 33 51 1443 2459 1712 138
Fragments arranged by size:
2459 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6150 6182 6150
Size: 32 6158
Tth111I GACn'n_nGTC
Cuts at: 1316 2744 1316
Size: 1428 4762
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2422 2530 2291
Size: 131 108 5951
Fragments arranged by size:
5951 131 108
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 3515 1870
Size: 1645 4545
XmnI GAAnn'nnTTC
Cuts at: 4319 4319
Size: 6190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2098 3998 276
Size: 53 83 186 936 564 1900 2468
Fragments arranged by size:
2468 1900 936 564 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SfoI SrfI
SwaI