Restriction map for
pFN21AE3596
(Circular) (Six-base) MAPSORT of: pFN21AE3596.seq Check: 46 from: 1 to: 7054
pFN21AE3596 7054 bp
With 182 enzymes: SgfI *
March 28, 2012 12:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4206 4206
Size: 7054
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4864 278
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4397 1937
Size: 2460 4594
Acc65I G'GTAC_C
Cuts at: 4375 4375
Size: 7054
AclI AA'CG_TT
Cuts at: 1563 5181 5554 1563
Size: 3618 373 3063
Fragments arranged by size:
3618 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3773 5117 6501 6769 1632
Size: 2141 1344 1384 268 1917
Fragments arranged by size:
2141 1917 1384 1344 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6857
AflIII A'CryG_T
Cuts at: 1203 5953 6930 1203
Size: 4750 977 1327
Fragments arranged by size:
4750 1327 977
AgeI A'CCGG_T
Cuts at: 2829 5885 2829
Size: 3056 3998
AhdI GACnn_n'nnGTC
Cuts at: 5783 5783
Size: 7054
AleI CACnn'nnGTG
Cuts at: 1166 2367 3653 1166
Size: 1201 1286 4567
Fragments arranged by size:
4567 1286 1201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4036 1184
Size: 2852 4202
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3672 3704 3672
Size: 32 7022
AlwNI CAG_nnn'CTG
Cuts at: 2929 2947 3589 3772 3982 4186 4797 6369 2929
Size: 18 642 183 210 204 611 1572 3614
Fragments arranged by size:
3614 1572 642 611 210 204 183 18
ApaLI G'TGCA_C
Cuts at: 5111 6267 5111
Size: 1156 5898
ApoI r'AATT_y
Cuts at: 1493 2315 2518 4363 4589 4741 6850 1493
Size: 822 203 1845 226 152 2109 1697
Fragments arranged by size:
2109 1845 1697 822 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 5608 160
Size: 872 4576 1606
Fragments arranged by size:
4576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
AvaI C'yCGr_G
Cuts at: 1870 1943 3071 4379 1870
Size: 73 1128 1308 4545
Fragments arranged by size:
4545 1308 1128 73
AvrII C'CTAG_G
Cuts at: 4330 4330
Size: 7054
BamHI G'GATC_C
Cuts at: 4384 4751 4384
Size: 367 6687
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2361 3124 3569 3596 4208
Size: 325 217 627 574 763 445 27 612
Cuts at: 4208 4375 5830 618
Size: 167 1455 1842
Fragments arranged by size:
1842 1455 763 627 612 574 445 325
217 167 27
BanII G_rGCy'C
Cuts at: 1391 2564 2850 3104 3231 3239 3564 3884 3908
Size: 1173 286 254 127 8 325 320 24
Cuts at: 3908 4122 4278 4373 1391
Size: 214 156 95 4072
Fragments arranged by size:
4072 1173 325 320 286 254 214 156
127 95 24 8
BbeI G_GCGC'C
Cuts at: 3600 3600
Size: 7054
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3785 928
Size: 971 1886 4197
Fragments arranged by size:
4197 1886 971
BbvCI CC'TCA_GC
Cuts at: 3773 4171 3773
Size: 398 6656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2791 2825 5245 5279 1903
Size: 34 854 34 2420 34 3678
Fragments arranged by size:
3678 2420 854 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4946 6156 4946
Size: 1210 5844
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7054
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3082 3860 3918
Size: 107 122 71 631 605 1410 778 58
Cuts at: 3918 3942 4175 4259 4282 5665 136
Size: 24 233 84 23 1383 1525
Fragments arranged by size:
1525 1410 1383 778 631 605 233 122
107 84 71 58 24 23
BglII A'GATC_T
Cuts at: 7049 7049
Size: 7054
BlpI GC'TnA_GC
Cuts at: 2844 4471 2844
Size: 1627 5427
Bme1580I G_kGCm'C
Cuts at: 1790 2364 3176 4239 5115 6271 1790
Size: 574 812 1063 876 1156 2573
Fragments arranged by size:
2573 1156 1063 876 812 574
BmgBI CAC'GTC
Cuts at: 1318 4113 1318
Size: 2795 4259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3052 3647 4785
Size: 416 53 83 399 24 1635 595 1138
Cuts at: 4785 5743 442
Size: 958 1753
Fragments arranged by size:
1753 1635 1138 958 595 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2647 2735 3191 3215 3967 3971 3977 5714
Size: 1408 88 456 24 752 4 6 1737
Cuts at: 5714 1239
Size: 2579
Fragments arranged by size:
2579 1737 1408 752 456 88 24 6
4
Bpu10I CC'TnA_GC
Cuts at: 3666 3773 3878 4171 3666
Size: 107 105 293 6549
Fragments arranged by size:
6549 293 107 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2733 3023 3050 3729 5179 6044 6342 2438
Size: 295 290 27 679 1450 865 298 3150
Fragments arranged by size:
3150 1450 865 679 298 295 290 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3439 5717 882
Size: 617 1940 2278 2219
Fragments arranged by size:
2278 2219 1940 617
BsaAI yAC'GTr
Cuts at: 493 1204 2550 4845 493
Size: 711 1346 2295 2702
Fragments arranged by size:
2702 2295 1346 711
BsaBI GATnn'nnATC
Cuts at: 7048 7048
Size: 7054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3597 3725 4861 5243
Size: 53 83 186 936 2064 128 1136 382
Cuts at: 5243 5878 275
Size: 635 1451
Fragments arranged by size:
2064 1451 1136 936 635 382 186 128
83 53
BsaWI w'CCGG_w
Cuts at: 2829 5487 5885 6159 6306 6717 2829
Size: 2658 398 274 147 411 3166
Fragments arranged by size:
3166 2658 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3672 3702 1178
Size: 30 165 30 2269 30 4530
Fragments arranged by size:
4530 2269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3014 3356 3383 3386 3696 3974 4097 1186
Size: 1828 342 27 3 310 278 123 4143
Fragments arranged by size:
4143 1828 342 310 278 123 27 3
BseYI C'CCAG_C
Cuts at: 2613 2929 3398 3592 4035 4097 6257 6753 2613
Size: 316 469 194 443 62 2160 496 2914
Fragments arranged by size:
2914 2160 496 469 443 316 194 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3215 3882 3899 3996 4184 4370 1163
Size: 573 1479 667 17 97 188 186 3847
Fragments arranged by size:
3847 1479 667 573 188 186 97 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2412 4008 4504 5265 6293 1540
Size: 275 186 411 1596 496 761 1028 2301
Fragments arranged by size:
2301 1596 1028 761 496 411 275 186
BsiHKAI G_wGCw'C
Cuts at: 3104 3231 3239 5115 5200 6271 3104
Size: 127 8 1876 85 1071 3887
Fragments arranged by size:
3887 1876 1071 127 85 8
BsmI GAATG_Cn'
Cuts at: 3058 4565 4658 3058
Size: 1507 93 5454
Fragments arranged by size:
5454 1507 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3481 3481
Size: 7054
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2564 2850 3104 3176 3231 3239
Size: 399 574 200 286 254 72 55 8
Cuts at: 3239 3564 3884 3908 4122 4239 4278 4373 5115
Size: 325 320 24 214 117 39 95 742
Cuts at: 5115 5200 6271 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 574 399 325 320 286
254 214 200 117 95 85 72 55
39 24 8
BspEI T'CCGG_A
Cuts at: 6717 6717
Size: 7054
BspHI T'CATG_A
Cuts at: 4943 6673 4943
Size: 1730 5324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2149 2697 3169 4206 4342 4409 844
Size: 1305 548 472 1037 136 67 3489
Fragments arranged by size:
3489 1305 1037 548 472 136 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3034 4093 4941 1394
Size: 429 272 939 1059 848 3507
Fragments arranged by size:
3507 1059 939 848 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 5549 5723 65
Size: 1183 4301 174 1396
Fragments arranged by size:
4301 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2829 2958 3929 4039 4151 4211 5698
Size: 110 1019 129 971 110 112 60 1487
Cuts at: 5698 5885 1700
Size: 187 2869
Fragments arranged by size:
2869 1487 1019 971 187 129 112 110
110 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7054
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7054
BssSI C'ACGA_G
Cuts at: 5114 6126 5114
Size: 1012 6042
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3500 3982 794
Size: 2706 482 3866
Fragments arranged by size:
3866 2706 482
BstBI TT'CG_AA
Cuts at: 5858 5858
Size: 7054
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7054
BstYI r'GATC_y
Cuts at: 1972 2817 4384 4751 5136 5153 6605 6691 6703
Size: 845 1567 367 385 17 1452 86 12
Cuts at: 6703 7049 1972
Size: 346 1977
Fragments arranged by size:
1977 1567 1452 845 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3129 3129
Size: 7054
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2412 3089 3489 3555 513
Size: 552 939 33 375 677 400 66 4012
Fragments arranged by size:
4012 939 677 552 400 375 66 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2794 487
Size: 683 56 1568 4747
Fragments arranged by size:
4747 1568 683 56
BtsI GCAGTG_nn'
Cuts at: 2978 3251 4570 5364 5384 2978
Size: 273 1319 794 20 4648
Fragments arranged by size:
4648 1319 794 273 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7019
DraI TTT'AAA
Cuts at: 4358 4358
Size: 7054
DraIII CAC_nnn'GTG
Cuts at: 2553 2553
Size: 7054
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2974 4156 6061 809
Size: 507 1658 1182 1905 1802
Fragments arranged by size:
1905 1802 1658 1182 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2409 3426 3576
Size: 54 1425 50 275 18 579 1017 150
Cuts at: 3576 4005 4251 4501 5390 7019 8
Size: 429 246 250 889 1629 43
Fragments arranged by size:
1629 1425 1017 889 579 429 275 250
246 150 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2409 4005 4501 1537
Size: 275 597 1596 496 4090
Fragments arranged by size:
4090 1596 597 496 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4984 1353
Size: 3631 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2759 3634 5641 6015 6161 387
Size: 1064 138 1170 875 2007 374 146 1280
Fragments arranged by size:
2007 1280 1170 1064 875 374 146 138
EcoICRI GAG'CTC
Cuts at: 3102 3229 3237 3102
Size: 127 8 6919
Fragments arranged by size:
6919 127 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2647 2735 3191 3215 3773 3967 3971
Size: 393 1015 88 456 24 558 194 4
Cuts at: 3971 3977 5117 5714 6501 6769 1239
Size: 6 1140 597 787 268 1524
Fragments arranged by size:
1524 1140 1015 787 597 558 456 393
268 194 88 24 6 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3132 3368 3428 3956 4349 3132
Size: 236 60 528 393 5837
Fragments arranged by size:
5837 528 393 236 60
EcoO109I rG'GnC_Cy
Cuts at: 2449 2609 2861 3847 4030 4312 2449
Size: 160 252 986 183 282 5191
Fragments arranged by size:
5191 986 282 252 183 160
EcoRI G'AATT_C
Cuts at: 4363 4741 4363
Size: 378 6676
FspI TGC'GCA
Cuts at: 5560 5560
Size: 7054
HaeII r_GCGC'y
Cuts at: 1678 2930 3600 6201 1678
Size: 1252 670 2601 2531
Fragments arranged by size:
2601 2531 1252 670
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2528 2560 5695 5727 5769
Size: 32 135 32 956 32 3135 32 42
Cuts at: 5769 5801 1373
Size: 32 2626
Fragments arranged by size:
3135 2626 956 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4398 4644 669
Size: 988 281 2460 246 3079
Fragments arranged by size:
3079 2460 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2240 2267 748
Size: 1492 27 5535
Fragments arranged by size:
5535 1492 27
HpaI GTT'AAC
Cuts at: 4644 4644
Size: 7054
KasI G'GCGC_C
Cuts at: 3596 3596
Size: 7054
KpnI G_GTAC'C
Cuts at: 4379 4379
Size: 7054
MfeI C'AATT_G
Cuts at: 4653 4653
Size: 7054
MluI A'CGCG_T
Cuts at: 6930 6930
Size: 7054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1998 2379 2925 3181 3708 6168 6352 1305
Size: 693 381 546 256 527 2460 184 2007
Fragments arranged by size:
2460 2007 693 546 527 381 256 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4253 10
Size: 54 1425 343 2421 2811
Fragments arranged by size:
2811 2421 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 2780 3653 5012 5371 5530 518
Size: 648 1201 413 873 1359 359 159 2042
Fragments arranged by size:
2042 1359 1201 873 648 413 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2233 2617 2707 3091 3276 3282 3557
Size: 433 194 384 90 384 185 6 275
Cuts at: 3557 3772 4010 4126 4175 4232 4470 5147 6295
Size: 215 238 116 49 57 238 677 1148
Cuts at: 6295 6540 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 433 384 384 275 245
238 238 215 194 185 116 90 57
49 6
NaeI GCC'GGC
Cuts at: 3931 4041 3931
Size: 110 6944
NarI GG'CG_CC
Cuts at: 3597 3597
Size: 7054
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5563
Fragments arranged by size:
5563 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7054
NgoMIV G'CCGG_C
Cuts at: 3929 4039 3929
Size: 110 6944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7054
NotI GC'GGCC_GC
Cuts at: 4501 4501
Size: 7054
NspI r_CATG'y
Cuts at: 1132 1229 2299 2779 4412 5957 1132
Size: 97 1070 480 1633 1545 2229
Fragments arranged by size:
2229 1633 1545 1070 480 97
PasI CC'CwG_GG
Cuts at: 1348 2101 2947 4079 4279 1348
Size: 753 846 1132 200 4123
Fragments arranged by size:
4123 1132 846 753 200
PciI A'CATG_T
Cuts at: 5953 5953
Size: 7054
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 1108
Size: 1173 5881
PfoI T'CCnGG_A
Cuts at: 3708 4185 3708
Size: 477 6577
PmeI GTTT'AAAC
Cuts at: 4358 4358
Size: 7054
PmlI CAC'GTG
Cuts at: 2550 2550
Size: 7054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3672 3704 5123 5155 6636 6668 3672
Size: 32 1419 32 1481 32 4058
Fragments arranged by size:
4058 1481 1419 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 2861 3847 4312 2449
Size: 412 986 465 5191
Fragments arranged by size:
5191 986 465 412
PshAI GACnn'nnGTC
Cuts at: 1613 3525 1613
Size: 1912 5142
PsiI TTA'TAA
Cuts at: 4624 4624
Size: 7054
PstI C_TGCA'G
Cuts at: 830 3246 3281 3505 3663 4149 4293 4406 830
Size: 2416 35 224 158 486 144 113 3478
Fragments arranged by size:
3478 2416 486 224 158 144 113 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7054
PvuII CAG'CTG
Cuts at: 2233 2617 3276 3772 2233
Size: 384 659 496 5515
Fragments arranged by size:
5515 659 496 384
SacI G_AGCT'C
Cuts at: 3104 3231 3239 3104
Size: 127 8 6919
Fragments arranged by size:
6919 127 8
SacII CC_GC'GG
Cuts at: 2040 3092 3558 2040
Size: 1052 466 5536
Fragments arranged by size:
5536 1052 466
SalI G'TCGA_C
Cuts at: 1936 4396 1936
Size: 2460 4594
SanDI GG'GwC_CC
Cuts at: 2861 2861
Size: 7054
SbfI CC_TGCA'GG
Cuts at: 4406 4406
Size: 7054
ScaI AGT'ACT
Cuts at: 2420 5302 7014 2420
Size: 2882 1712 2460
Fragments arranged by size:
2882 2460 1712
SexAI A'CCwGG_T
Cuts at: 2274 2274
Size: 7054
SfcI C'TryA_G
Cuts at: 826 1045 3242 3277 3501 3659 4145 4289 4402
Size: 219 2197 35 224 158 486 144 113
Cuts at: 4402 5537 6218 6409 826
Size: 1135 681 191 1471
Fragments arranged by size:
2197 1471 1135 681 486 224 219 191
158 144 113 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 4259 4259
Size: 7054
SfoI GGC'GCC
Cuts at: 3598 3598
Size: 7054
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
SgrAI Cr'CCGG_yG
Cuts at: 1700 2829 1700
Size: 1129 5925
SmaI CCC'GGG
Cuts at: 1872 3073 4381 1872
Size: 1201 1308 4545
Fragments arranged by size:
4545 1308 1201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 2748 3029 3038 3744 5158
Size: 197 926 510 295 281 9 706 1414
Cuts at: 5158 6059 6321 820
Size: 901 262 1553
Fragments arranged by size:
1553 1414 926 901 706 510 295 281
262 197 9
SnaBI TAC'GTA
Cuts at: 493 4845 493
Size: 4352 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7054
SphI G_CATG'C
Cuts at: 1132 2779 4412 1132
Size: 1647 1633 3774
Fragments arranged by size:
3774 1647 1633
SspI AAT'ATT
Cuts at: 5 52 4978 5
Size: 47 4926 2081
Fragments arranged by size:
4926 2081 47
StuI AGG'CCT
Cuts at: 1517 3758 3912 1517
Size: 2241 154 4659
Fragments arranged by size:
4659 2241 154
StyI C'CwwG_G
Cuts at: 513 1065 2004 3189 4330 4493 513
Size: 552 939 1185 1141 163 3074
Fragments arranged by size:
3074 1185 1141 939 552 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2474 5081 5098 5251 5436 5881 325
Size: 1255 894 2607 17 153 185 445 1498
Fragments arranged by size:
2607 1498 1255 894 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 5300 7012
Size: 274 80 33 51 1443 441 2882 1712
Cuts at: 7012 96
Size: 138
Fragments arranged by size:
2882 1712 1443 441 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3481 3513 7014 7046 3481
Size: 32 3501 32 3489
Fragments arranged by size:
3501 3489 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7054
XbaI T'CTAG_A
Cuts at: 4390 4390
Size: 7054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3799 3799
Size: 7054
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7054
XmaI C'CCGG_G
Cuts at: 1870 3071 4379 1870
Size: 1201 1308 4545
Fragments arranged by size:
4545 1308 1201
XmnI GAAnn'nnTTC
Cuts at: 2390 5183 2390
Size: 2793 4261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4862 276
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BfrBI BplI BsiWI BstXI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SapI SrfI SwaI