Restriction map for
pFN21AE3591
(Circular) (Six-base) MAPSORT of: pFN21AE3591.seq Check: 7984 from: 1 to: 5974
pFN21AE3591 5974 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3246 3246
Size: 5974
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4037 5421 5689 1632
Size: 2405 1384 268 1917
Fragments arranged by size:
2405 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 2366 4873 5850 1203
Size: 1163 2507 977 1327
Fragments arranged by size:
2507 1327 1163 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 3071 1166
Size: 1905 4069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2326 1184
Size: 1142 4832
AlwNI CAG_nnn'CTG
Cuts at: 2388 2700 3015 3250 3261 3717 5289 2388
Size: 312 315 235 11 456 1572 3073
Fragments arranged by size:
3073 1572 456 315 312 235 11
ApaLI G'TGCA_C
Cuts at: 2109 4031 5187 2109
Size: 1922 1156 2896
Fragments arranged by size:
2896 1922 1156
ApoI r'AATT_y
Cuts at: 1493 2793 3283 3509 3661 5770 1493
Size: 1300 490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1300 490 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3116 3299 1870
Size: 73 1173 183 4545
Fragments arranged by size:
4545 1173 183 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3029 3062 3029
Size: 33 5941
BamHI G'GATC_C
Cuts at: 2991 3304 3671 2991
Size: 313 367 5294
Fragments arranged by size:
5294 367 313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3172 3293 1391
Size: 1781 121 4072
Fragments arranged by size:
4072 1781 121
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2300 928
Size: 971 401 4602
Fragments arranged by size:
4602 971 401
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 2503 1548
Size: 955 5019
BfrBI ATG'CAT
Cuts at: 2892 2892
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2170 5969 2170
Size: 3799 2175
BlpI GC'TnA_GC
Cuts at: 2252 3171 3391 2252
Size: 919 220 4835
Fragments arranged by size:
4835 919 220
Bme1580I G_kGCm'C
Cuts at: 1790 2113 4035 5191 1790
Size: 323 1922 1156 2573
Fragments arranged by size:
2573 1922 1156 323
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 3062 3128 4634 1239
Size: 1259 564 66 1506 2579
Fragments arranged by size:
2579 1506 1259 564 66
Bpu10I CC'TnA_GC
Cuts at: 2378 2954 3002 2378
Size: 576 48 5350
Fragments arranged by size:
5350 576 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4099 4964 5262 4099
Size: 865 298 4811
Fragments arranged by size:
4811 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2367 2663 3765 493
Size: 711 1163 296 1102 2702
Fragments arranged by size:
2702 1163 1102 711 296
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3050 1186
Size: 1864 4110
BseYI C'CCAG_C
Cuts at: 5177 5673 5177
Size: 496 5478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2563 2705 2981 3168 3274 1163
Size: 573 827 142 276 187 106 3863
Fragments arranged by size:
3863 827 573 276 187 142 106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2113 4035 4120 5191 2113
Size: 1922 85 1071 2896
Fragments arranged by size:
2896 1922 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 3485 3578 2894
Size: 591 93 5290
Fragments arranged by size:
5290 591 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 3172 3293 4035 4120 5191 1391
Size: 399 323 1059 121 742 85 1071 2174
Fragments arranged by size:
2174 1071 1059 742 399 323 121 85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2822 3127 3246 3329 844
Size: 1978 305 119 83 3489
Fragments arranged by size:
3489 1978 305 119 83
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2706 4618 4805 1700
Size: 110 896 1912 187 2869
Fragments arranged by size:
2869 1912 896 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 3139 1184
Size: 1955 4019
BstYI r'GATC_y
Cuts at: 1972 2170 2410 2878 2991 3304 3671 4056 4073
Size: 198 240 468 113 313 367 385 17
Cuts at: 4073 5525 5611 5623 5969 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 468 385 367 346 313 240
198 113 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2243 2570 2243
Size: 327 5647
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2242 487
Size: 683 56 1016 4219
Fragments arranged by size:
4219 1016 683 56
BtsI GCAGTG_nn'
Cuts at: 3234 3490 4284 4304 3234
Size: 256 794 20 4904
Fragments arranged by size:
4904 794 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3019 3421 4310
Size: 54 1425 50 275 18 1189 402 889
Cuts at: 4310 5939 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1189 889 402 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3904 1353
Size: 2551 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2498 3062 3128 4037 4634 5421 5689
Size: 393 866 564 66 909 597 787 268
Cuts at: 5689 1239
Size: 1524
Fragments arranged by size:
1524 909 866 787 597 564 393 268
66
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 3115 5121 1678
Size: 1437 2006 2531
Fragments arranged by size:
2531 2006 1437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 3154 3186 4615
Size: 32 135 32 830 32 720 32 1429
Cuts at: 4615 4647 4689 4721 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1429 830 720 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3027 5088 5272 1305
Size: 1722 2061 184 2007
Fragments arranged by size:
2061 2007 1722 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3021 10
Size: 54 1425 343 1189 2963
Fragments arranged by size:
2963 1425 1189 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3071 3932 4291 4450 518
Size: 648 1905 861 359 159 2042
Fragments arranged by size:
2042 1905 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3390 4067 5215 5460 1606
Size: 1784 677 1148 245 2120
Fragments arranged by size:
2120 1784 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NsiI A_TGCA'T
Cuts at: 2894 2894
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3332 4877 1132
Size: 97 2103 1545 2229
Fragments arranged by size:
2229 2103 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2767 1348
Size: 1419 4555
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2127 2866 1108
Size: 1019 739 4216
Fragments arranged by size:
4216 1019 739
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PmlI CAC'GTG
Cuts at: 2367 2367
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspXI vC'TCGA_Gb
Cuts at: 3116 3116
Size: 5974
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2711 3322 4457 5138 5329 826
Size: 219 1666 611 1135 681 191 1471
Fragments arranged by size:
1666 1471 1135 681 611 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3116 4078 4979 5241 820
Size: 197 926 1173 962 901 262 1553
Fragments arranged by size:
1553 1173 962 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 2073 3898 5
Size: 47 2021 1825 2081
Fragments arranged by size:
2081 2021 1825 47
StuI AGG'CCT
Cuts at: 1517 2031 2553 3105 1517
Size: 514 522 552 4386
Fragments arranged by size:
4386 552 522 514
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 3413 513
Size: 552 939 117 1292 3074
Fragments arranged by size:
3074 1292 939 552 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4001 4018 4171 4356 4801 325
Size: 1255 2421 17 153 185 445 1498
Fragments arranged by size:
2421 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5934 5966 5934
Size: 32 5942
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 3116 1943
Size: 1173 4801
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 2074 4103 2074
Size: 2029 3945
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI