(Circular) (Six-base) MAPSORT of: pFN21AE3591.seq Check: 7984 from: 1 to: 5974 pFN21AE3591 5974 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3246 3246 Size: 5974 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4037 5421 5689 1632 Size: 2405 1384 268 1917 Fragments arranged by size: 2405 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 2366 4873 5850 1203 Size: 1163 2507 977 1327 Fragments arranged by size: 2507 1327 1163 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 3071 1166 Size: 1905 4069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2326 1184 Size: 1142 4832 AlwNI CAG_nnn'CTG Cuts at: 2388 2700 3015 3250 3261 3717 5289 2388 Size: 312 315 235 11 456 1572 3073 Fragments arranged by size: 3073 1572 456 315 312 235 11 ApaLI G'TGCA_C Cuts at: 2109 4031 5187 2109 Size: 1922 1156 2896 Fragments arranged by size: 2896 1922 1156 ApoI r'AATT_y Cuts at: 1493 2793 3283 3509 3661 5770 1493 Size: 1300 490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1300 490 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3116 3299 1870 Size: 73 1173 183 4545 Fragments arranged by size: 4545 1173 183 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3029 3062 3029 Size: 33 5941 BamHI G'GATC_C Cuts at: 2991 3304 3671 2991 Size: 313 367 5294 Fragments arranged by size: 5294 367 313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3172 3293 1391 Size: 1781 121 4072 Fragments arranged by size: 4072 1781 121 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2300 928 Size: 971 401 4602 Fragments arranged by size: 4602 971 401 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 2503 1548 Size: 955 5019 BfrBI ATG'CAT Cuts at: 2892 2892 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2170 5969 2170 Size: 3799 2175 BlpI GC'TnA_GC Cuts at: 2252 3171 3391 2252 Size: 919 220 4835 Fragments arranged by size: 4835 919 220 Bme1580I G_kGCm'C Cuts at: 1790 2113 4035 5191 1790 Size: 323 1922 1156 2573 Fragments arranged by size: 2573 1922 1156 323 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3705 4663 442 Size: 416 53 83 399 24 2288 958 1753 Fragments arranged by size: 2288 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2498 3062 3128 4634 1239 Size: 1259 564 66 1506 2579 Fragments arranged by size: 2579 1506 1259 564 66 Bpu10I CC'TnA_GC Cuts at: 2378 2954 3002 2378 Size: 576 48 5350 Fragments arranged by size: 5350 576 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4099 4964 5262 4099 Size: 865 298 4811 Fragments arranged by size: 4811 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2367 2663 3765 493 Size: 711 1163 296 1102 2702 Fragments arranged by size: 2702 1163 1102 711 296 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3050 1186 Size: 1864 4110 BseYI C'CCAG_C Cuts at: 5177 5673 5177 Size: 496 5478 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2563 2705 2981 3168 3274 1163 Size: 573 827 142 276 187 106 3863 Fragments arranged by size: 3863 827 573 276 187 142 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2113 4035 4120 5191 2113 Size: 1922 85 1071 2896 Fragments arranged by size: 2896 1922 1071 85 BsmI GAATG_Cn' Cuts at: 2894 3485 3578 2894 Size: 591 93 5290 Fragments arranged by size: 5290 591 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 3172 3293 4035 4120 5191 1391 Size: 399 323 1059 121 742 85 1071 2174 Fragments arranged by size: 2174 1071 1059 742 399 323 121 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2822 3127 3246 3329 844 Size: 1978 305 119 83 3489 Fragments arranged by size: 3489 1978 305 119 83 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2706 4618 4805 1700 Size: 110 896 1912 187 2869 Fragments arranged by size: 2869 1912 896 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 3139 1184 Size: 1955 4019 BstYI r'GATC_y Cuts at: 1972 2170 2410 2878 2991 3304 3671 4056 4073 Size: 198 240 468 113 313 367 385 17 Cuts at: 4073 5525 5611 5623 5969 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 468 385 367 346 313 240 198 113 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2243 2570 2243 Size: 327 5647 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2242 487 Size: 683 56 1016 4219 Fragments arranged by size: 4219 1016 683 56 BtsI GCAGTG_nn' Cuts at: 3234 3490 4284 4304 3234 Size: 256 794 20 4904 Fragments arranged by size: 4904 794 256 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3019 3421 4310 Size: 54 1425 50 275 18 1189 402 889 Cuts at: 4310 5939 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1189 889 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3904 1353 Size: 2551 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2498 3062 3128 4037 4634 5421 5689 Size: 393 866 564 66 909 597 787 268 Cuts at: 5689 1239 Size: 1524 Fragments arranged by size: 1524 909 866 787 597 564 393 268 66 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2272 2272 Size: 5974 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 3115 5121 1678 Size: 1437 2006 2531 Fragments arranged by size: 2531 2006 1437 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 3154 3186 4615 Size: 32 135 32 830 32 720 32 1429 Cuts at: 4615 4647 4689 4721 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1429 830 720 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3027 5088 5272 1305 Size: 1722 2061 184 2007 Fragments arranged by size: 2061 2007 1722 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3021 10 Size: 54 1425 343 1189 2963 Fragments arranged by size: 2963 1425 1189 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3071 3932 4291 4450 518 Size: 648 1905 861 359 159 2042 Fragments arranged by size: 2042 1905 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3390 4067 5215 5460 1606 Size: 1784 677 1148 245 2120 Fragments arranged by size: 2120 1784 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NsiI A_TGCA'T Cuts at: 2894 2894 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3332 4877 1132 Size: 97 2103 1545 2229 Fragments arranged by size: 2229 2103 1545 97 PasI CC'CwG_GG Cuts at: 1348 2767 1348 Size: 1419 4555 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2127 2866 1108 Size: 1019 739 4216 Fragments arranged by size: 4216 1019 739 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PmlI CAC'GTG Cuts at: 2367 2367 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspXI vC'TCGA_Gb Cuts at: 3116 3116 Size: 5974 PstI C_TGCA'G Cuts at: 830 3326 830 Size: 2496 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2711 3322 4457 5138 5329 826 Size: 219 1666 611 1135 681 191 1471 Fragments arranged by size: 1666 1471 1135 681 611 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3116 4078 4979 5241 820 Size: 197 926 1173 962 901 262 1553 Fragments arranged by size: 1553 1173 962 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 2073 3898 5 Size: 47 2021 1825 2081 Fragments arranged by size: 2081 2021 1825 47 StuI AGG'CCT Cuts at: 1517 2031 2553 3105 1517 Size: 514 522 552 4386 Fragments arranged by size: 4386 552 522 514 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 3413 513 Size: 552 939 117 1292 3074 Fragments arranged by size: 3074 1292 939 552 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4001 4018 4171 4356 4801 325 Size: 1255 2421 17 153 185 445 1498 Fragments arranged by size: 2421 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4220 5932 96 Size: 274 80 33 51 1443 2243 1712 138 Fragments arranged by size: 2243 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5934 5966 5934 Size: 32 5942 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 3116 1943 Size: 1173 4801 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 2074 4103 2074 Size: 2029 3945 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI