Restriction map for
pFN21AE3588
(Circular) (Six-base) MAPSORT of: pFN21AE3588.seq Check: 6200 from: 1 to: 7063
pFN21AE3588 7063 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4095 4873 278
Size: 53 83 186 936 2559 778 2468
Fragments arranged by size:
2559 2468 936 778 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3927 4406 1937
Size: 1990 479 4594
Fragments arranged by size:
4594 1990 479
Acc65I G'GTAC_C
Cuts at: 3024 4384 3024
Size: 1360 5703
AclI AA'CG_TT
Cuts at: 1563 5190 5563 1563
Size: 3627 373 3063
Fragments arranged by size:
3627 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2288 2392 2411 5126 6510 6778 1632
Size: 578 78 104 19 2715 1384 268 1917
Fragments arranged by size:
2715 1917 1384 578 268 104 78 19
AfeI AGC'GCT
Cuts at: 3566 3566
Size: 7063
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6866
AflIII A'CryG_T
Cuts at: 1203 5962 6939 1203
Size: 4759 977 1327
Fragments arranged by size:
4759 1327 977
AgeI A'CCGG_T
Cuts at: 3021 5894 3021
Size: 2873 4190
AhdI GACnn_n'nnGTC
Cuts at: 5792 5792
Size: 7063
AleI CACnn'nnGTG
Cuts at: 1166 2214 3233 1166
Size: 1048 1019 4996
Fragments arranged by size:
4996 1048 1019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7063
AlwNI CAG_nnn'CTG
Cuts at: 2245 2714 3067 3292 4015 4806 6378 2245
Size: 469 353 225 723 791 1572 2930
Fragments arranged by size:
2930 1572 791 723 469 353 225
ApaI G_GGCC'C
Cuts at: 4165 4165
Size: 7063
ApaLI G'TGCA_C
Cuts at: 5120 6276 5120
Size: 1156 5907
ApoI r'AATT_y
Cuts at: 1493 2299 4372 4598 4750 6859 1493
Size: 806 2073 226 152 2109 1697
Fragments arranged by size:
2109 2073 1697 806 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5617 160
Size: 872 4585 1606
Fragments arranged by size:
4585 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7063
AvaI C'yCGr_G
Cuts at: 1870 1943 3700 4388 1870
Size: 73 1757 688 4545
Fragments arranged by size:
4545 1757 688 73
BamHI G'GATC_C
Cuts at: 4393 4760 4393
Size: 367 6696
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2435 3024 3043 4384 5839
Size: 325 217 627 648 589 19 1341 1455
Cuts at: 5839 618
Size: 1842
Fragments arranged by size:
1842 1455 1341 648 627 589 325 217
19
BanII G_rGCy'C
Cuts at: 1391 3315 4165 4382 1391
Size: 1924 850 217 4072
Fragments arranged by size:
4072 1924 850 217
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2890 3459 3750 928
Size: 971 991 569 291 4241
Fragments arranged by size:
4241 991 971 569 291
BbvCI CC'TCA_GC
Cuts at: 3662 3689 3662
Size: 27 7036
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 5254 5288 1903
Size: 34 1862 34 1421 34 3678
Fragments arranged by size:
3678 1862 1421 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4955 6165 4955
Size: 1210 5853
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7063
BfrBI ATG'CAT
Cuts at: 4317 4317
Size: 7063
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3359 5674 136
Size: 107 122 71 631 605 1687 2315 1525
Fragments arranged by size:
2315 1687 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7058 7058
Size: 7063
BlpI GC'TnA_GC
Cuts at: 2418 3596 4480 2418
Size: 1178 884 5001
Fragments arranged by size:
5001 1178 884
Bme1580I G_kGCm'C
Cuts at: 1790 4165 5124 6280 1790
Size: 2375 959 1156 2573
Fragments arranged by size:
2573 2375 1156 959
BmgBI CAC'GTC
Cuts at: 1318 3889 1318
Size: 2571 4492
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4794 5752 442
Size: 416 53 83 399 24 3377 958 1753
Fragments arranged by size:
3377 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7063
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3214 3214
Size: 7063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2241 2846 3044 3722 3844 5723 1239
Size: 1002 605 198 678 122 1879 2579
Fragments arranged by size:
2579 1879 1002 678 605 198 122
Bpu10I CC'TnA_GC
Cuts at: 3662 3689 3662
Size: 27 7036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 3493 5188 6053 6351 2122
Size: 1371 1695 865 298 2834
Fragments arranged by size:
2834 1695 1371 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5726 882
Size: 617 4227 2219
Fragments arranged by size:
4227 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4854 493
Size: 711 3650 2702
Fragments arranged by size:
3650 2702 711
BsaBI GATnn'nnATC
Cuts at: 7057 7057
Size: 7063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3504 4092 4870 5252
Size: 53 83 186 936 1971 588 778 382
Cuts at: 5252 5887 275
Size: 635 1451
Fragments arranged by size:
1971 1451 936 778 635 588 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3021 3437 5496 5894 6168 6315 6726 3021
Size: 416 2059 398 274 147 411 3358
Fragments arranged by size:
3358 2059 416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6838
Fragments arranged by size:
6838 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 2618 2651 2666 3049 3809 3986 4102
Size: 906 526 33 15 383 760 177 116
Cuts at: 4102 4343 4346 1186
Size: 241 3 3903
Fragments arranged by size:
3903 906 760 526 383 241 177 116
33 15 3
BseYI C'CCAG_C
Cuts at: 2184 2320 2769 3067 3285 3349 3805 3899 4287
Size: 136 449 298 218 64 456 94 388
Cuts at: 4287 6266 6762 2184
Size: 1979 496 2485
Fragments arranged by size:
2485 1979 496 456 449 388 298 218
136 94 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2927 2981 3076 3308 3316 1163
Size: 573 1191 54 95 232 8 4910
Fragments arranged by size:
4910 1191 573 232 95 54 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4513 5274 6302 1540
Size: 275 186 2512 761 1028 2301
Fragments arranged by size:
2512 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 3315 5124 5209 6280 2124
Size: 1191 1809 85 1071 2907
Fragments arranged by size:
2907 1809 1191 1071 85
BsmI GAATG_Cn'
Cuts at: 4574 4667 4574
Size: 93 6970
BsmBI CGTCTCn'nnnn_
Cuts at: 2259 3202 3791 2259
Size: 943 589 5531
Fragments arranged by size:
5531 943 589
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 3315 4165 4382 5124 5209 6280
Size: 399 334 1191 850 217 742 85 1071
Cuts at: 6280 1391
Size: 2174
Fragments arranged by size:
2174 1191 1071 850 742 399 334 217
85
BspEI T'CCGG_A
Cuts at: 6726 6726
Size: 7063
BspHI T'CATG_A
Cuts at: 4952 6682 4952
Size: 1730 5333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3036 4418 844
Size: 2192 1382 3489
Fragments arranged by size:
3489 2192 1382
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 2422 2484 4950 1394
Size: 429 190 409 62 2466 3507
Fragments arranged by size:
3507 2466 429 409 190 62
BsrDI GCAATG_nn'
Cuts at: 65 1248 3946 5558 5732 65
Size: 1183 2698 1612 174 1396
Fragments arranged by size:
2698 1612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3021 5707 5894 1700
Size: 110 1211 2686 187 2869
Fragments arranged by size:
2869 2686 1211 187 110
BsrGI T'GTAC_A
Cuts at: 96 2176 96
Size: 2080 4983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7063
BssSI C'ACGA_G
Cuts at: 2510 5123 6135 2510
Size: 2613 1012 3438
Fragments arranged by size:
3438 2613 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3078 794
Size: 2284 4779
BstBI TT'CG_AA
Cuts at: 2304 5867 2304
Size: 3563 3500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7063
BstXI CCAn_nnnn'nTGG
Cuts at: 3235 4060 3235
Size: 825 6238
BstYI r'GATC_y
Cuts at: 1972 2710 3114 3675 4032 4393 4760 5145 5162
Size: 738 404 561 357 361 367 385 17
Cuts at: 5162 6614 6700 6712 7058 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 738 561 404 385 367 361
357 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2519 4102 2519
Size: 1583 5480
BtgI C'CryG_G
Cuts at: 513 1065 2004 2471 2502 3006 3047 3921 4143
Size: 552 939 467 31 504 41 874 222
Cuts at: 4143 513
Size: 3433
Fragments arranged by size:
3433 939 874 552 504 467 222 41
31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2734 3058 487
Size: 683 56 1508 324 4492
Fragments arranged by size:
4492 1508 683 324 56
BtsI GCAGTG_nn'
Cuts at: 2566 3771 4285 4579 5373 5393 2566
Size: 1205 514 294 794 20 4236
Fragments arranged by size:
4236 1205 794 514 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7063
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4125 4160 529
Size: 35 3561 35 3432
Fragments arranged by size:
3561 3432 35 35
DraI TTT'AAA
Cuts at: 4367 4367
Size: 7063
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2704 6070 809
Size: 507 1388 3366 1802
Fragments arranged by size:
3366 1802 1388 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2249 3381
Size: 54 1425 50 275 18 333 86 1132
Cuts at: 3381 4510 5399 7028 8
Size: 1129 889 1629 43
Fragments arranged by size:
1629 1425 1132 1129 889 333 275 86
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4510 1537
Size: 275 2698 4090
Fragments arranged by size:
4090 2698 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2462 3582 4993 1353
Size: 1109 1120 1411 3423
Fragments arranged by size:
3423 1411 1120 1109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2453 3190 3328 3721 5650 6024
Size: 1064 138 864 737 138 393 1929 374
Cuts at: 6024 6170 387
Size: 146 1280
Fragments arranged by size:
1929 1280 1064 864 737 393 374 146
138 138
EcoICRI GAG'CTC
Cuts at: 3313 3313
Size: 7063
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2241 2288 2392 2411 2846 3044
Size: 393 578 31 47 104 19 435 198
Cuts at: 3044 3722 3844 5126 5723 6510 6778 1239
Size: 678 122 1282 597 787 268 1524
Fragments arranged by size:
1524 1282 787 678 597 578 435 393
268 198 122 104 47 31 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2633 2633
Size: 7063
EcoO109I rG'GnC_Cy
Cuts at: 2082 2194 3169 3250 3430 3995 4003 4161 4162
Size: 112 975 81 180 565 8 158 1
Cuts at: 4162 2082
Size: 4983
Fragments arranged by size:
4983 975 565 180 158 112 81 8
1
EcoRI G'AATT_C
Cuts at: 4372 4750 4372
Size: 378 6685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3508 3508
Size: 7063
FspI TGC'GCA
Cuts at: 5569 5569
Size: 7063
HaeII r_GCGC'y
Cuts at: 1678 3568 4022 6210 1678
Size: 1890 454 2188 2531
Fragments arranged by size:
2531 2188 1890 454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3824 3856 4187 4219 5704
Size: 32 135 32 2252 32 331 32 1485
Cuts at: 5704 5736 5778 5810 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2252 1485 331 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4407 4653 669
Size: 988 281 2469 246 3079
Fragments arranged by size:
3079 2469 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7063
HpaI GTT'AAC
Cuts at: 4653 4653
Size: 7063
KpnI G_GTAC'C
Cuts at: 3028 4388 3028
Size: 1360 5703
MfeI C'AATT_G
Cuts at: 4662 4662
Size: 7063
MluI A'CGCG_T
Cuts at: 6939 6939
Size: 7063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6177 6361 1305
Size: 4872 184 2007
Fragments arranged by size:
4872 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3383 10
Size: 54 1425 343 1551 3690
Fragments arranged by size:
3690 1551 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2214 3233 5021 5380 5539 518
Size: 648 1048 1019 1788 359 159 2042
Fragments arranged by size:
2042 1788 1048 1019 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2188 2536 2782 3049 3064 3424 3550 3903
Size: 582 348 246 267 15 360 126 353
Cuts at: 3903 4479 5156 6304 6549 1606
Size: 576 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 582 576 360 353 348
267 246 245 126 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5572
Fragments arranged by size:
5572 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7063
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7063
NotI GC'GGCC_GC
Cuts at: 4510 4510
Size: 7063
NsiI A_TGCA'T
Cuts at: 4319 4319
Size: 7063
NspI r_CATG'y
Cuts at: 1132 1229 2229 4421 5966 1132
Size: 97 1000 2192 1545 2229
Fragments arranged by size:
2229 2192 1545 1000 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7063
PciI A'CATG_T
Cuts at: 5962 5962
Size: 7063
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2220 3067 1108
Size: 1112 847 5104
Fragments arranged by size:
5104 1112 847
PfoI T'CCnGG_A
Cuts at: 2238 3126 3738 2238
Size: 888 612 5563
Fragments arranged by size:
5563 888 612
PmeI GTTT'AAAC
Cuts at: 4367 4367
Size: 7063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5132 5164 6645 6677 5132
Size: 32 1481 32 5518
Fragments arranged by size:
5518 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 3169 3250 3430 3995 4003 2194
Size: 975 81 180 565 8 5254
Fragments arranged by size:
5254 975 565 180 81 8
PshAI GACnn'nnGTC
Cuts at: 1613 3263 1613
Size: 1650 5413
PsiI TTA'TAA
Cuts at: 4633 4633
Size: 7063
PspOMI G'GGCC_C
Cuts at: 4161 4161
Size: 7063
PstI C_TGCA'G
Cuts at: 830 2541 4415 830
Size: 1711 1874 3478
Fragments arranged by size:
3478 1874 1711
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7063
PvuII CAG'CTG
Cuts at: 2188 2536 2782 3064 3424 2188
Size: 348 246 282 360 5827
Fragments arranged by size:
5827 360 348 282 246
SacI G_AGCT'C
Cuts at: 3315 3315
Size: 7063
SacII CC_GC'GG
Cuts at: 3050 3050
Size: 7063
SalI G'TCGA_C
Cuts at: 1936 4405 1936
Size: 2469 4594
SanDI GG'GwC_CC
Cuts at: 3995 3995
Size: 7063
SapI GCTCTTCn'nnn_
Cuts at: 2462 2462
Size: 7063
SbfI CC_TGCA'GG
Cuts at: 4415 4415
Size: 7063
ScaI AGT'ACT
Cuts at: 5311 7023 5311
Size: 1712 5351
SexAI A'CCwGG_T
Cuts at: 2196 3171 2196
Size: 975 6088
SfcI C'TryA_G
Cuts at: 826 1045 2029 2148 2537 3517 3601 4411 5546
Size: 219 984 119 389 980 84 810 1135
Cuts at: 5546 6227 6418 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 984 980 810 681 389 219
191 119 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7063
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7063
SmaI CCC'GGG
Cuts at: 1872 4390 1872
Size: 2518 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2101 3472 5167 6068 6330 820
Size: 197 926 158 1371 1695 901 262 1553
Fragments arranged by size:
1695 1553 1371 926 901 262 197 158
SnaBI TAC'GTA
Cuts at: 493 4854 493
Size: 4361 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7063
SphI G_CATG'C
Cuts at: 1132 4421 1132
Size: 3289 3774
SspI AAT'ATT
Cuts at: 5 52 4987 5
Size: 47 4935 2081
Fragments arranged by size:
4935 2081 47
StuI AGG'CCT
Cuts at: 1517 2349 2396 1517
Size: 832 47 6184
Fragments arranged by size:
6184 832 47
StyI C'CwwG_G
Cuts at: 513 1065 2004 3204 3510 4502 513
Size: 552 939 1200 306 992 3074
Fragments arranged by size:
3074 1200 992 939 552 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2423 4043 5090 5107 5260 5445 5890
Size: 1255 843 1620 1047 17 153 185 445
Cuts at: 5890 325
Size: 1498
Fragments arranged by size:
1620 1498 1255 1047 843 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2176 5309 7021
Size: 274 80 33 51 1443 199 3133 1712
Cuts at: 7021 96
Size: 138
Fragments arranged by size:
3133 1712 1443 274 199 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2540 2572 2814 2846 4114 4146 7023 7055 2540
Size: 32 242 32 1268 32 2877 32 2548
Fragments arranged by size:
2877 2548 1268 242 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7063
XbaI T'CTAG_A
Cuts at: 4399 4399
Size: 7063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4141 4141
Size: 7063
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7063
XmaI C'CCGG_G
Cuts at: 1870 4388 1870
Size: 2518 4545
XmnI GAAnn'nnTTC
Cuts at: 2978 3491 5192 2978
Size: 513 1701 4849
Fragments arranged by size:
4849 1701 513
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4093 4871 276
Size: 53 83 186 936 2559 778 2468
Fragments arranged by size:
2559 2468 936 778 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SfiI SfoI
SrfI SwaI