Restriction map for
pFN21AE3587
(Circular) (Six-base) MAPSORT of: pFN21AE3587.seq Check: 4816 from: 1 to: 5419
pFN21AE3587 5419 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2142 2149 2306 2676 2142
Size: 7 157 370 4885
Fragments arranged by size:
4885 370 157 7
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3229 278
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2762 1937
Size: 825 4594
Acc65I G'GTAC_C
Cuts at: 2740 2740
Size: 5419
AclI AA'CG_TT
Cuts at: 1563 3546 3919 1563
Size: 1983 373 3063
Fragments arranged by size:
3063 1983 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3482 4866 5134 1632
Size: 1850 1384 268 1917
Fragments arranged by size:
1917 1850 1384 268
AfeI AGC'GCT
Cuts at: 2324 2387 2324
Size: 63 5356
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5222
AflIII A'CryG_T
Cuts at: 1203 4318 5295 1203
Size: 3115 977 1327
Fragments arranged by size:
3115 1327 977
AgeI A'CCGG_T
Cuts at: 4250 4250
Size: 5419
AhdI GACnn_n'nnGTC
Cuts at: 4148 4148
Size: 5419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5419
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5419
AlwNI CAG_nnn'CTG
Cuts at: 2134 3162 4734 2134
Size: 1028 1572 2819
Fragments arranged by size:
2819 1572 1028
ApaI G_GGCC'C
Cuts at: 2079 2222 2241 2567 2079
Size: 143 19 326 4931
Fragments arranged by size:
4931 326 143 19
ApaLI G'TGCA_C
Cuts at: 3476 4632 3476
Size: 1156 4263
ApoI r'AATT_y
Cuts at: 1493 2728 2954 3106 5215 1493
Size: 1235 226 152 2109 1697
Fragments arranged by size:
2109 1697 1235 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3973 160
Size: 872 2941 1606
Fragments arranged by size:
2941 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
AvaI C'yCGr_G
Cuts at: 1870 1943 2209 2222 2744 1870
Size: 73 266 13 522 4545
Fragments arranged by size:
4545 522 266 73 13
BamHI G'GATC_C
Cuts at: 2749 3116 2749
Size: 367 5052
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2617 2740 4195 618
Size: 325 217 627 830 123 1455 1842
Fragments arranged by size:
1842 1455 830 627 325 217 123
BanII G_rGCy'C
Cuts at: 1391 2079 2222 2241 2567 2738 1391
Size: 688 143 19 326 171 4072
Fragments arranged by size:
4072 688 326 171 143 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2598 928
Size: 971 699 3749
Fragments arranged by size:
3749 971 699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3610 3644 1903
Size: 34 1673 34 3678
Fragments arranged by size:
3678 1673 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3311 4521 3311
Size: 1210 4209
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5419
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2286 4030
Size: 107 122 71 631 605 337 277 1744
Cuts at: 4030 136
Size: 1525
Fragments arranged by size:
1744 1525 631 605 337 277 122 107
71
BglII A'GATC_T
Cuts at: 5414 5414
Size: 5419
BlpI GC'TnA_GC
Cuts at: 2836 2836
Size: 5419
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2079 2222 2241 2567 2622 3480 4636
Size: 256 33 143 19 326 55 858 1156
Cuts at: 4636 1790
Size: 2573
Fragments arranged by size:
2573 1156 858 326 256 143 55 33
19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2545 3150 4108
Size: 416 53 83 399 24 1128 605 958
Cuts at: 4108 442
Size: 1753
Fragments arranged by size:
1753 1128 958 605 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4079 1239
Size: 2840 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2648 3544 4409 4707 2648
Size: 896 865 298 3360
Fragments arranged by size:
3360 896 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4082 882
Size: 617 2583 2219
Fragments arranged by size:
2583 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2263 3210 493
Size: 711 1059 947 2702
Fragments arranged by size:
2702 1059 947 711
BsaBI GATnn'nnATC
Cuts at: 5413 5413
Size: 5419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3226 3608 4243 275
Size: 53 83 186 936 1693 382 635 1451
Fragments arranged by size:
1693 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3852 4250 4524 4671 5082 3852
Size: 398 274 147 411 4189
Fragments arranged by size:
4189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 1178
Size: 30 165 30 1026 30 4138
Fragments arranged by size:
4138 1026 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5419
BseYI C'CCAG_C
Cuts at: 2089 2325 4622 5118 2089
Size: 236 2297 496 2390
Fragments arranged by size:
2390 2297 496 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 2450 1163
Size: 573 441 273 4132
Fragments arranged by size:
4132 573 441 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2342 2869 3630 4658 1540
Size: 275 186 341 527 761 1028 2301
Fragments arranged by size:
2301 1028 761 527 341 275 186
BsiHKAI G_wGCw'C
Cuts at: 3480 3565 4636 3480
Size: 85 1071 4263
Fragments arranged by size:
4263 1071 85
BsmI GAATG_Cn'
Cuts at: 2930 3023 2930
Size: 93 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2079 2222 2241 2567 2622 2738
Size: 399 256 33 143 19 326 55 116
Cuts at: 2738 3480 3565 4636 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 326 256 143 116
85 55 33 19
BspEI T'CCGG_A
Cuts at: 5082 5082
Size: 5419
BspHI T'CATG_A
Cuts at: 2700 3308 5038 2700
Size: 608 1730 3081
Fragments arranged by size:
3081 1730 608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 2149 2306 2472 2676 2774 844
Size: 1298 7 157 166 204 98 3489
Fragments arranged by size:
3489 1298 204 166 157 98 7
BsrBI CCG'CTC
Cuts at: 1394 1823 3306 1394
Size: 429 1483 3507
Fragments arranged by size:
3507 1483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3914 4088 65
Size: 1183 2666 174 1396
Fragments arranged by size:
2666 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 2337 2423 2533 4063 4250 1700
Size: 110 287 240 86 110 1530 187 2869
Fragments arranged by size:
2869 1530 287 240 187 110 110 86
BsrGI T'GTAC_A
Cuts at: 96 2268 96
Size: 2172 3247
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5419
BssSI C'ACGA_G
Cuts at: 2039 2429 3479 4491 2039
Size: 390 1050 1012 2967
Fragments arranged by size:
2967 1050 1012 390
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2263 794
Size: 1469 3950
BstBI TT'CG_AA
Cuts at: 4223 4223
Size: 5419
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5419
BstYI r'GATC_y
Cuts at: 1972 2749 3116 3501 3518 4970 5056 5068 5414
Size: 777 367 385 17 1452 86 12 346
Cuts at: 5414 1972
Size: 1977
Fragments arranged by size:
1977 1452 777 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2613 2613
Size: 5419
BtgI C'CryG_G
Cuts at: 513 1065 2004 2559 2679 513
Size: 552 939 555 120 3253
Fragments arranged by size:
3253 939 555 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2499 2671 487
Size: 683 56 1273 172 3235
Fragments arranged by size:
3235 1273 683 172 56
BtsI GCAGTG_nn'
Cuts at: 2935 3729 3749 2935
Size: 794 20 4605
Fragments arranged by size:
4605 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5384
DraI TTT'AAA
Cuts at: 2723 2723
Size: 5419
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4426 809
Size: 507 3110 1802
Fragments arranged by size:
3110 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2339 2425 2676
Size: 54 1425 50 275 18 509 86 251
Cuts at: 2676 2866 3755 5384 8
Size: 190 889 1629 43
Fragments arranged by size:
1629 1425 889 509 275 251 190 86
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2339 2866 1537
Size: 275 527 527 4090
Fragments arranged by size:
4090 527 527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2627 3349 1353
Size: 1274 722 3423
Fragments arranged by size:
3423 1274 722
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4006 4380 4526 387
Size: 1064 138 2417 374 146 1280
Fragments arranged by size:
2417 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3482 4079 4866 5134 1239
Size: 393 1850 597 787 268 1524
Fragments arranged by size:
1850 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2075 2219 2237 2564 2075
Size: 144 18 327 4930
Fragments arranged by size:
4930 327 144 18
EcoRI G'AATT_C
Cuts at: 2728 3106 2728
Size: 378 5041
FspI TGC'GCA
Cuts at: 3925 3925
Size: 5419
HaeII r_GCGC'y
Cuts at: 1678 2090 2326 2389 2397 4566 1678
Size: 412 236 63 8 2169 2531
Fragments arranged by size:
2531 2169 412 236 63 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4060 4092 4134 4166 1373
Size: 32 135 32 2488 32 42 32 2626
Fragments arranged by size:
2626 2488 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2763 3009 669
Size: 988 281 825 246 3079
Fragments arranged by size:
3079 988 825 281 246
HindIII A'AGCT_T
Cuts at: 748 2624 748
Size: 1876 3543
HpaI GTT'AAC
Cuts at: 3009 3009
Size: 5419
KpnI G_GTAC'C
Cuts at: 2744 2744
Size: 5419
MfeI C'AATT_G
Cuts at: 3018 3018
Size: 5419
MluI A'CGCG_T
Cuts at: 5295 5295
Size: 5419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2148 4533 4717 1305
Size: 843 2385 184 2007
Fragments arranged by size:
2385 2007 843 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3597
Fragments arranged by size:
3597 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3377 3736 3895 518
Size: 648 2211 359 159 2042
Fragments arranged by size:
2211 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2053 2062 2643 2835 3512 4660 4905
Size: 414 33 9 581 192 677 1148 245
Cuts at: 4905 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 581 414 245 192 33
9
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 5419
NcoI C'CATG_G
Cuts at: 513 1065 2004 2559 513
Size: 552 939 555 3373
Fragments arranged by size:
3373 939 555 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5419
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 5419
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5419
NotI GC'GGCC_GC
Cuts at: 2866 2866
Size: 5419
NspI r_CATG'y
Cuts at: 1132 1229 2105 2777 4322 1132
Size: 97 876 672 1545 2229
Fragments arranged by size:
2229 1545 876 672 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5419
PciI A'CATG_T
Cuts at: 4318 4318
Size: 5419
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5419
PmeI GTTT'AAAC
Cuts at: 2723 2723
Size: 5419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 5001 5033 3488
Size: 32 1481 32 3874
Fragments arranged by size:
3874 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5419
PsiI TTA'TAA
Cuts at: 2989 2989
Size: 5419
PspOMI G'GGCC_C
Cuts at: 2075 2218 2237 2563 2075
Size: 143 19 326 4931
Fragments arranged by size:
4931 326 143 19
PstI C_TGCA'G
Cuts at: 830 2771 830
Size: 1941 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5419
SalI G'TCGA_C
Cuts at: 1936 2761 1936
Size: 825 4594
SapI GCTCTTCn'nnn_
Cuts at: 2627 2627
Size: 5419
SbfI CC_TGCA'GG
Cuts at: 2771 2771
Size: 5419
ScaI AGT'ACT
Cuts at: 2435 3667 5379 2435
Size: 1232 1712 2475
Fragments arranged by size:
2475 1712 1232
SfcI C'TryA_G
Cuts at: 826 1045 2389 2767 3902 4583 4774 826
Size: 219 1344 378 1135 681 191 1471
Fragments arranged by size:
1471 1344 1135 681 378 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5419
SmaI CCC'GGG
Cuts at: 1872 2224 2746 1872
Size: 352 522 4545
Fragments arranged by size:
4545 522 352
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2627 3523 4424 4686 820
Size: 197 926 684 896 901 262 1553
Fragments arranged by size:
1553 926 901 896 684 262 197
SnaBI TAC'GTA
Cuts at: 493 3210 493
Size: 2717 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5419
SphI G_CATG'C
Cuts at: 1132 2105 2777 1132
Size: 973 672 3774
Fragments arranged by size:
3774 973 672
SspI AAT'ATT
Cuts at: 5 52 3343 5
Size: 47 3291 2081
Fragments arranged by size:
3291 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5419
StyI C'CwwG_G
Cuts at: 513 1065 2004 2071 2559 2858 513
Size: 552 939 67 488 299 3074
Fragments arranged by size:
3074 939 552 488 299 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3446 3463 3616 3801 4246 325
Size: 1255 1866 17 153 185 445 1498
Fragments arranged by size:
1866 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 2268 2433
Size: 274 80 33 51 1443 132 159 165
Cuts at: 2433 3665 5377 96
Size: 1232 1712 138
Fragments arranged by size:
1712 1443 1232 274 165 159 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5379 5411 5379
Size: 32 5387
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5419
XbaI T'CTAG_A
Cuts at: 2755 2755
Size: 5419
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5419
XmaI C'CCGG_G
Cuts at: 1870 2222 2744 1870
Size: 352 522 4545
Fragments arranged by size:
4545 522 352
XmnI GAAnn'nnTTC
Cuts at: 2492 3548 2492
Size: 1056 4363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3227 276
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI