Restriction map for
pFN21AE3585
(Circular) (Six-base) MAPSORT of: pFN21AE3585.seq Check: 2596 from: 1 to: 6298
pFN21AE3585 6298 bp
With 182 enzymes: SgfI *
March 28, 2012 12:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4108 278
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3641 1937
Size: 1704 4594
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4361 5745 6013 1632
Size: 2729 1384 268 1917
Fragments arranged by size:
2729 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 5197 6174 1203
Size: 3994 977 1327
Fragments arranged by size:
3994 1327 977
AgeI A'CCGG_T
Cuts at: 2605 5129 2605
Size: 2524 3774
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 2606 1166
Size: 1440 4858
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6298
AlwNI CAG_nnn'CTG
Cuts at: 3566 4041 5613 3566
Size: 475 1572 4251
Fragments arranged by size:
4251 1572 475
ApaLI G'TGCA_C
Cuts at: 4355 5511 4355
Size: 1156 5142
ApoI r'AATT_y
Cuts at: 1493 3607 3833 3985 6094 1493
Size: 2114 226 152 2109 1697
Fragments arranged by size:
2114 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 2595 2880 3244 3336 3623 1870
Size: 73 652 285 364 92 287 4545
Fragments arranged by size:
4545 652 364 287 285 92 73
BamHI G'GATC_C
Cuts at: 3628 3995 3628
Size: 367 5931
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2386 3035 3619 5074
Size: 325 217 627 221 378 649 584 1455
Cuts at: 5074 618
Size: 1842
Fragments arranged by size:
1842 1455 649 627 584 378 325 221
217
BanII G_rGCy'C
Cuts at: 1391 3185 3617 1391
Size: 1794 432 4072
Fragments arranged by size:
4072 1794 432
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6298
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2552 2617 928
Size: 971 653 65 4609
Fragments arranged by size:
4609 971 653 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2368 2402 4489 4523 1903
Size: 34 431 34 2087 34 3678
Fragments arranged by size:
3678 2087 431 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4909 136
Size: 107 122 71 631 605 3237 1525
Fragments arranged by size:
3237 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3360 6293 3360
Size: 2933 3365
BlpI GC'TnA_GC
Cuts at: 3715 3715
Size: 6298
Bme1580I G_kGCm'C
Cuts at: 1790 4359 5515 1790
Size: 2569 1156 2573
Fragments arranged by size:
2573 2569 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4029 4987
Size: 416 53 83 399 24 2093 519 958
Cuts at: 4987 442
Size: 1753
Fragments arranged by size:
2093 1753 958 519 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2916 1056
Size: 1860 4438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2106 4958 1239
Size: 867 2852 2579
Fragments arranged by size:
2852 2579 867
Bpu10I CC'TnA_GC
Cuts at: 2661 3093 2661
Size: 432 5866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4423 5288 5586 4423
Size: 865 298 5135
Fragments arranged by size:
5135 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4961 882
Size: 617 3462 2219
Fragments arranged by size:
3462 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4089 493
Size: 711 2885 2702
Fragments arranged by size:
2885 2702 711
BsaBI GATnn'nnATC
Cuts at: 2645 6292 2645
Size: 3647 2651
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4105 4487 5122
Size: 53 83 186 936 476 2096 382 635
Cuts at: 5122 275
Size: 1451
Fragments arranged by size:
2096 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2064 2605 3130 4731 5129 5403 5550 5961 2064
Size: 541 525 1601 398 274 147 411 2401
Fragments arranged by size:
2401 1601 541 525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2208 2238 2711 2741 1178
Size: 30 165 30 805 30 473 30 4735
Fragments arranged by size:
4735 805 473 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2846 2849 3149 3164 3206 3371 1186
Size: 1162 498 3 300 15 42 165 4113
Fragments arranged by size:
4113 1162 498 300 165 42 15 3
BseYI C'CCAG_C
Cuts at: 2031 2367 2683 3020 3111 3176 5501 5997 2031
Size: 336 316 337 91 65 2325 496 2332
Fragments arranged by size:
2332 2325 496 337 336 316 91 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2727 1163
Size: 573 991 4734
Fragments arranged by size:
4734 991 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3748 4509 5537 1540
Size: 275 186 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2662 3243 3531 4359 4444 5515 2662
Size: 581 288 828 85 1071 3445
Fragments arranged by size:
3445 1071 828 581 288 85
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
BsmBI CGTCTCn'nnnn_
Cuts at: 2795 2795
Size: 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2662 3185 3243 3531 3617 4359 4444
Size: 399 872 523 58 288 86 742 85
Cuts at: 4444 5515 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 872 742 523 399 288 86
85 58
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 4187 5917 4187
Size: 1730 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3653 844
Size: 2809 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3469 4793 4967 65
Size: 1183 2221 1324 174 1396
Fragments arranged by size:
2221 1396 1324 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2605 4942 5129 1700
Size: 110 795 2337 187 2869
Fragments arranged by size:
2869 2337 795 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 2698 4358 5370 2698
Size: 1660 1012 3626
Fragments arranged by size:
3626 1660 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6298
BstBI TT'CG_AA
Cuts at: 5102 5102
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1184 2248 1184
Size: 1064 5234
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 3545 2200
Size: 1345 4953
BstYI r'GATC_y
Cuts at: 1972 2305 2363 3360 3628 3995 4380 4397 5849
Size: 333 58 997 268 367 385 17 1452
Cuts at: 5849 5935 5947 6293 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 997 385 367 346 333 268
86 58 17 12
Bsu36I CC'TnA_GG
Cuts at: 2457 2457
Size: 6298
BtgI C'CryG_G
Cuts at: 513 1065 2004 2390 513
Size: 552 939 386 4421
Fragments arranged by size:
4421 939 552 386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5559
Fragments arranged by size:
5559 683 56
BtsI GCAGTG_nn'
Cuts at: 3220 3235 3310 3376 3442 3556 3814 4608 4628
Size: 15 75 66 66 114 258 794 20
Cuts at: 4628 3220
Size: 4890
Fragments arranged by size:
4890 794 258 114 75 66 66 20
15
ClaI AT'CG_AT
Cuts at: 1554 2256 1554
Size: 702 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3451 3486 529
Size: 35 2887 35 3341
Fragments arranged by size:
3341 2887 35 35
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2246 5305 809
Size: 507 930 3059 1802
Fragments arranged by size:
3059 1802 930 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 3472 3745
Size: 54 1425 50 275 18 214 1428 273
Cuts at: 3745 4634 6263 8
Size: 889 1629 43
Fragments arranged by size:
1629 1428 1425 889 275 273 214 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3745 1537
Size: 275 1933 4090
Fragments arranged by size:
4090 1933 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 2793 3135 3141 3159 4228 1353
Size: 972 468 342 6 18 1069 3423
Fragments arranged by size:
3423 1069 972 468 342 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2984 4885 5259 5405 387
Size: 1064 138 1395 1901 374 146 1280
Fragments arranged by size:
1901 1395 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 4361 4958 5745 6013 1239
Size: 393 474 2255 597 787 268 1524
Fragments arranged by size:
2255 1524 787 597 474 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2344 2344
Size: 6298
EcoRI G'AATT_C
Cuts at: 3607 3985 3607
Size: 378 5920
EcoRV GAT'ATC
Cuts at: 2128 2128
Size: 6298
FspI TGC'GCA
Cuts at: 4804 4804
Size: 6298
HaeII r_GCGC'y
Cuts at: 1678 2012 3010 3504 5445 1678
Size: 334 998 494 1941 2531
Fragments arranged by size:
2531 1941 998 494 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2711 2713 2743 2745 3227
Size: 32 135 32 1139 2 30 2 482
Cuts at: 3227 3259 3383 3415 4939 4971 5013 5045 1373
Size: 32 124 32 1524 32 42 32 2626
Fragments arranged by size:
2626 1524 1139 482 135 124 42 32
32 32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3642 3888 669
Size: 988 281 1704 246 3079
Fragments arranged by size:
3079 1704 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6298
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2697 2961 5412 5596 1305
Size: 1392 264 2451 184 2007
Fragments arranged by size:
2451 2007 1392 264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2046 3474 10
Size: 54 1425 343 214 1428 2834
Fragments arranged by size:
2834 1428 1425 343 214 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 2606 4256 4615 4774 518
Size: 648 1051 389 1650 359 159 2042
Fragments arranged by size:
2042 1650 1051 648 389 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2312 2711 3456 3478 3714 4391 5539
Size: 429 277 399 745 22 236 677 1148
Cuts at: 5539 5784 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 745 677 429 399 277 245
236 22
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6298
NcoI C'CATG_G
Cuts at: 513 1065 2004 2390 513
Size: 552 939 386 4421
Fragments arranged by size:
4421 939 552 386
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NheI G'CTAG_C
Cuts at: 1052 2912 1052
Size: 1860 4438
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 3656 5201 1132
Size: 97 2427 1545 2229
Fragments arranged by size:
2427 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6298
PciI A'CATG_T
Cuts at: 5197 5197
Size: 6298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 4630
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PstI C_TGCA'G
Cuts at: 830 3562 3650 830
Size: 2732 88 3478
Fragments arranged by size:
3478 2732 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
PvuII CAG'CTG
Cuts at: 2312 2711 2312
Size: 399 5899
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 2108 4546 6258 2108
Size: 2438 1712 2148
Fragments arranged by size:
2438 2148 1712
SfcI C'TryA_G
Cuts at: 826 1045 2793 3558 3646 4781 5462 5653 826
Size: 219 1748 765 88 1135 681 191 1471
Fragments arranged by size:
1748 1471 1135 765 681 219 191 88
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6298
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 2605 1700
Size: 905 5393
SmaI CCC'GGG
Cuts at: 1872 3338 3625 1872
Size: 1466 287 4545
Fragments arranged by size:
4545 1466 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4402 5303 5565 820
Size: 197 926 2459 901 262 1553
Fragments arranged by size:
2459 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 3656 1132
Size: 2524 3774
SrfI GCCC'GGGC
Cuts at: 3338 3338
Size: 6298
SspI AAT'ATT
Cuts at: 5 52 4222 5
Size: 47 4170 2081
Fragments arranged by size:
4170 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6298
StyI C'CwwG_G
Cuts at: 513 1065 2004 2199 2390 2691 3015 3427 3737
Size: 552 939 195 191 301 324 412 310
Cuts at: 3737 513
Size: 3074
Fragments arranged by size:
3074 939 552 412 324 310 301 195
191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4325 4342 4495 4680 5125 325
Size: 1255 2745 17 153 185 445 1498
Fragments arranged by size:
2745 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2184 2409
Size: 274 80 33 51 1443 129 78 225
Cuts at: 2409 2886 4544 6256 96
Size: 477 1658 1712 138
Fragments arranged by size:
1712 1658 1443 477 274 225 138 129
80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6258 6290 6258
Size: 32 6266
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6298
XbaI T'CTAG_A
Cuts at: 2302 3634 2302
Size: 1332 4966
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2039 2039
Size: 6298
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3336 3623 1870
Size: 1466 287 4545
Fragments arranged by size:
4545 1466 287
XmnI GAAnn'nnTTC
Cuts at: 2174 4427 2174
Size: 2253 4045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4106 276
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SwaI